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烤烟种子成熟过程中三种酶的动态及其作用
引用本文:刘敬业,卢江平,等.烤烟种子成熟过程中三种酶的动态及其作用[J].昆明师范高等专科学校学报,1999,21(4):48-51.
作者姓名:刘敬业  卢江平
作者单位:[1]昆明师专生物系,昆明650031 [2]云南省烟草研究所,玉溪653100
摘    要:研究了烤烟种子在成熟过程中硝酸还原酶(NR)、多酚氧化酶(PPO)、过氧化物酶(POD)同工酶活性的变化。结果表明:NR、PPO、POD参与了烤烟种子的成熟过程,并调控有关代谢与生化反应。不同品种与成熟时期,各种酶活性变化有差异,但总的变化趋势则基本一致。NR活性以红花大金元最高,K326次之,G-28最低。PPO活性褐熟期前K326高,G-28次之,以红花大金元活性最低。POD同工酶谱带以红花大金元消减速率最快,但各品种都种子成熟而谱带减少,最后趋于消失。

关 键 词:烤烟  种子  成熟期  酶活性  繁育技术  过氧化物酶

Three Enzymes Dynamics and Functions During the Seed Maturing of Flue-Sured Tobacco
Liu Jingye, Wang Dingkang,Lu Jianping ,Yan Pinggeng,Yu Guiqing,Qiu Ling.Three Enzymes Dynamics and Functions During the Seed Maturing of Flue-Sured Tobacco[J].Journal of Kunming Teachers College,1999,21(4):48-51.
Authors:Liu Jingye  Wang Dingkang  Lu Jianping  Yan Pinggeng  Yu Guiqing  Qiu Ling
Abstract:This article is about a study on the activity changes of NR(nitrate redutase)PPO (poyphenoe ox-idase)and POD(peroxidase) imenzyme during the seed maturing of flue-cured tobacco. The results showed that NR, PPO, POD, engaged met in the seed rippening of flue-cured tobacco and they regutated the reated metablsism and biochemical reactions. klthough there were different maturing stages, the genereal changing tendencies were basically in consistent with the highest NR activity beloged to Gold Mammotyh, next K326 the lowest went to G - 28; before brown maturing stage K326 had the highest PPO activity. Gold Mammoth had the fast PPO activity. Gold Mammoth had the fastest reduction rate of POD iaxnzymeband, but as the seed getting mature, the band getting less and nearly disapeard at the end in all three vari-eties.
Keywords:seed of flue-cured tobacoo  ripening period  enzyme activity
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