By reviewing the theory and current situation of popular science industrial cluster, this paper sorts out the function and value of science museum resource elements, and puts forward the cluster operation mode of multiple popular science organizations based on common popular science goal. Combining with the practical case of Yangtze Delta Alliance for Popular Science Museums, this paper discusses how the main resource elements promote the formation of science museum cluster. On the basis of analyzing the problems, the development strategy of regional cluster of science museums in the future is proposed.