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1.
4-氯-2,6-双吡啶双氧钒化合物降低1型糖尿病大鼠血糖的生物学作用 总被引:1,自引:0,他引:1
通过1型和2型糖尿病动物模型的研究发现过渡金属元素钒(V)具有降糖作用。有机钒化合物与无机钒相比,其副作用低、体内吸收率和生物利用度高。本文主要探讨了四价有机钒化合物4-氯-2,6-双吡啶双氧钒化合物(4-chloro-2,6-dipicolinatodioxovanadium(Ⅳ),V4dipic-Cl)对链脲佐菌素(STZ,55 mg/kg)诱导的1型糖尿病大鼠的生物学作用。大鼠通过自由饮水的方式口服V4dipic-Cl(0.5 mg/ml)8天。结果表明,V4dipic-Cl组的大鼠血糖和血清碱性磷酸酶(ALP)活性显著低于糖尿病对照组,并且其葡萄糖耐量水平得到明显改善。但有机配体H2dipic-Cl对糖尿病大鼠的血糖和血清ALP活性的影响不显著。结果提示V4dipic-Cl具有降低糖尿病大鼠的血糖,提高葡萄糖耐量和改善肝功能的作用。 相似文献
2.
Alireza Nakhaee Mohammad Bokaeian Mohsen Saravani Ali Farhangi Azim Akbarzadeh 《Indian journal of clinical biochemistry : IJCB》2009,24(4):419-425
In traditional medicine, Eucalyptus globulus (eucalyptus) was used for the treatment of diabetes mellitus. Hyperglycemia in
diabetes has been associated with increased formation of reactive oxygen species (ROS) and oxidative damage to tissue compounds.
The aim of this study was to evaluate the effects of eucalyptus in the diet (20 g/Kg) and drinking water (2.5 g/L) on lipid
peroxidation, protein oxidation and antioxidant power in plasma and liver homogenate, as well as glycated-Hb (HbA1C) of blood in streptozotocin-induced diabetic rats for a period of 4 weeks. Diabetes induced in rats by a single intraperitoneal
injection of streptozotocin (STZ, 65 mg/Kg). At the end of the treatment period, the level of plasma glucose, plasma and liver
malondialdehyde (MDA, the main product of lipid peroxidation), protein carbonyl (PC, one of the protein oxidation products)
and HbA1C increased and ferric reducing antioxidant power (FRAP) decreased in diabetic rats compared to normal rats. Eucalyptus administration
for 4 weeks caused a significant decrease in the plasma glucose levels, plasma and liver MDA, PC and HbA1C, also a concomitant increase in the levels of FRAP in diabetic treated rats. In conclusion, the present study showed that
eucalyptus posses antioxidant activities. Eucalyptus probably restores antioxidant power, due to the improved hyperglycemia
in streptozotocin-induced diabetic rats. 相似文献
3.
Abolfazl Nasiri Nasrin Ziamajidi Roghayeh Abbasalipourkabir Mohammad Taghi Goodarzi Massoud Saidijam Hamid Behrouj Sara Solemani Asl 《Indian journal of clinical biochemistry : IJCB》2017,32(3):329-336
One of the most important complications of diabetes is nephropathy. This study investigates the effects of aqueous garlic extract on inflammation and oxidative stress status in the kidneys of diabetic rats. Male rats were divided into four groups- control rats, diabetic rats, garlic extract-treated diabetic rats, garlic extract-treated normal rats. The glucose, urea, uric acid, and creatinine levels were measured in sera using colorimetric methods. To determine the oxidative stress condition in the kidney tissues, total antioxidant capacity (TAC), malondialdehyde (MDA), and total oxidant status (TOS) were measured using colorimetric methods. Inflammation status was evaluated by the determination of tumor necrosis factor-alpha (TNF-α) gene and protein expression using qRT-PCR and ELISA respectively, while nitric oxide (NO) level in these tissues was measured using the Griess method. Histological examination of Kidneys was carried out by H&E staining. The levels of glucose, urea, and uric acid were found to increase in the serum of diabetic rats and decrease in that of diabetic rats after treatment with garlic. Measurement of MDA, TOS, and TAC revealed oxidative stress in diabetic rats, which improved after receiving the extract. The NO and TNF-α protein levels in diabetic rats were higher than those in control rats. After treatment with garlic, the levels of TNF-α protein and NO became close to the normal levels. Histological results confirmed certain other data as well. Garlic has antioxidant properties; therefore, it can reduce oxidative stress, which plays an important role in the development of diabetic nephropathy. Reduction in oxidative stress has beneficial effects on inflammation because it leads to a decrease in the level of TNF-α. 相似文献
4.
Santosh Kumar Singh Prashant Kumar Rai Shikha Mehta Rakesh Kumar Singh Geeta Watal 《Indian journal of clinical biochemistry : IJCB》2009,24(4):410-413
The aim of the study was to ascertain the role of ethanolic extract of Cynodon dactylon against hepatic complications in streptozotocin
(STZ) induced type 2 diabetic models. Effect of the pre identified most effective dose of 500 mg/kg body weight was studied
on hepatic injury caused by chemically induced diabetes by 55 mg/kg body weight i.p. injection of STZ in male Wistar rats.
The dose of 500mg/kg body weight given once daily for 14 days reduced the levels of serum glutamate oxaloacetate transaminase,
serum glutamate pyruvate transaminase, alkaline phosphatase, creatinine and urine sugar significantly (P<0.05) with increase
in total protein, haemoglobin and body weight was increased. High LD50 validates its high margin of safety. 相似文献
5.
Mohamed Ahmed Abdelmoaty M. A. Ibrahim N. S. Ahmed M. A. Abdelaziz 《Indian journal of clinical biochemistry : IJCB》2010,25(2):188-192
Quercetin (QE), one of natural flavanoid group, was widely distributed as a secondary metabolite in plant kingdom. It has
been believed that oxidative stress plays a role in the pathogenesis of diabetes mellitus (DM). The aim of the present study
was the evaluation of possible effects of QE on blood glucose and antioxidant enzymes in experimental streptozotocin (STZ)-induced
diabetes in rats. STZ was injected intraperitoneally with single dose of 50 mg/kg for diabetes induction. QE (15 mg/kg bw
day, intraperitoneal (i.p.) injection) was injected for 3 days prior to STZ administration; these injections were continued
to the end of the study (for 25 days). Glucose tolerance test and random plasma glucose were done for all animals. Cellular
antioxidant enzymes such as glutathione peroxidase (GSHPx), superoxide dismutase (SOD) and catalase (CAT) activities were
measured in pancreatic homogenates. Quercetin had no effect on plasma glucose level of normal animals but its pre- treatment
was able to prevent diabetes induced by single intraperitoneal injection of streptozocintreated rats. Antioxidant enzyme activity
significantly decreased in STZ induced diabetic group. QE treatment significantly increased the antioxidant enzyme activities.
It could be concluded that quercetin, a flavonoid with antioxidant properties, exerting its beneficial antidiabetic effects. 相似文献
6.
D. Gomathi M. Kalaiselvi G. Ravikumar K. Devaki C. Uma 《Indian journal of clinical biochemistry : IJCB》2014,29(2):221-226
Diabetes mellitus is one of the most common endocrine metabolic disorders. Dual endocrine deficits of impaired insulin action (insulin resistance) and inadequate insulin secretion create an environment of chronic hyperglycemia and general metabolic disarray. Oxidative stress plays an important role in diabetic pathogenesis. Oxidative stress induced by streptozotocin (STZ) has been shown to damage pancreatic beta cell and produce hyperglycemia in rats. The present study was made to evaluate the antioxidant activity of ethanolic extract of the Evolvulus alsinoides in STZ induced rats. The antioxidant activities were done by using standard protocols. For histopathological analysis, the pancreatic tissues of all experimental groups were fixed with 10 % formalin for 24 h then the samples were stained with hematoxylin–eosin for the microscopic observation. Our results showed the significant decrease in lipid peroxidation and increases in the antioxidant (both enzymatic and nonenzymatic) levels after treatment with standard as well as the E. alsinoides. There is no significant difference between control and plant alone group rats. The histopathology reports also revealed non-toxic effect and protective effect of E. alsinoides in the kidney of STZ induced diabetic rats. Our result indicated that the E. alsinoides extract effectively increased the antioxidant level thereby it prevents oxidative stress during diabetes mellitus and also it showed the protective effect on kidney of STZ induced rats. Hence it can be used to maintain the antioxidant level during diabetes mellitus. 相似文献
7.
M. Halim Eshrat 《Indian journal of clinical biochemistry : IJCB》2003,18(2):54-63
InAyurvedic system of medicine in India, not only extracts of one plant or the other but also a combination of plant extracts are used
for the treatment of diabetes mellitus. The present paper reports the combined effect ofAbroma augusta andCoccinia indica known to be useful for the treatment of diabetes in Ayurveda on the fasting blood sugar, glucose tolerance and lipid profile
of Streptozotocin (STZ) induced albino rats. 300mg of water extract of the mixture of dried powdered roots ofA. augusta and leaves ofC. indica in equal proportions was given once daily for 8 weeks. After 8 weeks of treatment of Streptozotocin (STZ) diabetic rats,
the fasting blood sugar came down to almost normal value and improvement in glucose tolerance and serum lipid profile were
also observed. 相似文献
8.
STZ诱导的糖尿病对骨密度和血清睾酮的影响(英文) 总被引:2,自引:0,他引:2
目的:探讨链脲霉素(STZ)诱导的糖尿病对大鼠骨代谢的影响及机制。材料与方法:雄性Wistar大鼠随机分成3组: 正常组(n = 6),糖尿病组(n = 5)和胰岛素治疗糖尿病组 (n = 5)。大鼠尾静脉一次性注射STZ(50 mg/kg 体重),选择空腹12 h血糖大于12 mmol/L的大鼠为本实验所需的糖尿病模型。治疗组大鼠每天在同一时间给予1.8-2.2U的胰岛素。实验周期为持续32天。采用双能X线骨吸收法(DEXA)测定股骨密度,ELISA法测定血清雄性激素睾酮水平,生化分析仪测定血清碱性磷酸酶、钙、磷浓度。结果:糖尿病大鼠的股骨密度和血清睾酮均显著低于正常组(P < 0.01),血清总碱性磷酸酶明显高于正常组和胰岛素治疗组(P < 0.01)。三组之间血钙和血磷的水平无明显差异。胰岛素治疗后糖尿病大鼠的骨密度、血清睾酮与总碱性磷酸酶得到明显的改善。结论:糖尿病严重影响骨密度,胰岛素缺乏及雄性激素降低是导致糖尿病性骨质疏松重要原因。胰岛素治疗能预防骨流失和提高血清睾酮浓度。 相似文献
9.
Mahalingam Gayathri Krishnan Kannabiran 《Indian journal of clinical biochemistry : IJCB》2008,23(4):394-400
The aim of the present study was to evaluate the antidiabetic and ameliorative potential of aqueous extract of Ficus bengalensis
bark in streptozotocin induced diabetic rats. The effect of oral administration of aqueous extract of F. bengalensis bark
on blood glucose, serum electrolytes, serum glycolytic enzymes, liver microsomal protein, hepatic cytochrome P-450 dependent
monooxygenase enzymes and lipid peroxidation in liver and kidney of streptozotocin -induced diabetic rats was studied. Oral
administration of Ficus bengalensis to fed, fasted and glucose loaded diabetic rats significantly [F > 0.05 (ANOVA) and P<
0.05 (DMRT)] decreased the blood glucose level at 5 hrs and restored the levels of serum electrolytes, glycolytic enzymes
and hepatic cytochrome P-450 dependent enzyme systems and decreased the formation of liver and kidney lipid peroxides at the
end of 12 weeks. Further, the aqueous extract of Ficus bengalensis at a dose of 500mg/kg/day exhibits significant antidiabetic
and ameliorative activity as evidenced by histological studies in normal and Ficus bengalensis treated streptozotocin induced
diabetic rats. On the basis of our findings, it could be used as an antidiabetic and ameliorative agent for better management
of diabetes mellitus. 相似文献
10.
Fouzia Rashid M. Kaleem Sheema B. Bano 《Indian journal of clinical biochemistry : IJCB》2005,20(1):109-114
The present study is related to the comparative effects of fish oil and olive oil supplementation on gentamicin induced nephrotoxicity
in rats. Three treatment groups (Pretrement, Co-treatment and post treatment) were chosen for the study. Nephrotoxicity in
rats was induced by intraperitonial administration of gentamicin (80 mg/kg/d) for 3,5,7,10,& 12 consecutive days. The animals
were sacrificed 12 hrs after last treatment in each group. The maximum nephrotoxicity was developed on 10 days treatment of
gentamicin. For each group a control group was taken without any oil or gentamicin treatment. Beneficial effects of oils were
evidenced by reduced serum urea and creatinine concentrations in the group receiving oils compared to the non oil treatment
animals receiving gentamicin only. Further, the changed values of alkaline phosphatase and acid phosphatase activity retumed
to normal in kidney and liver tissue homogenates after fish and olive oil treatment. In this study, it was found that co-treatment
of fish and olive oil is more effective antagonist of gentamicin induced nephrotoxicity. However fish oil was found to be
more effective. Hypercholesteromia associated with gentamicin induced nephrotoxicity is also lowered by oil supplementations.
The beneficial effects of these oils are due to counteracting effect of the biochemical alterations induced by the drug. 相似文献
11.
Merin Iype Subramanya Upadhya Sharmila Upadhya Gopalakrishna Bhat 《Indian journal of clinical biochemistry : IJCB》2008,23(4):401-403
Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various
studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model
was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used
a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia
B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical
procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were
measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant
elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared
to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained
for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating
to renal failure in the field of research. 相似文献
12.
Mst. Marium Begum Zakia Sultana Md. Ershad Ali Md. Safkath Ibne Jami Proma Khondkar Md. Masuduzzaman Khan Md. Mominul Haque 《Indian journal of clinical biochemistry : IJCB》2014,29(4):452-461
High blood glucose level, elevated level of liver enzyme, necrosis and shrinkage of islets of Langerhans has been implicated in the pathogenesis of type 2 diabetes. High blood glucose cause oxidative stress, production of free radical as well as elevated SGPT and SGOT level. Both glibenclamide and simvastatin in fixed dose used as antihyperglycemic antidyslipidemic and antioxidative agents for type 2 diabetes treatment. This study therefore aimed to evaluate the antihyperglycemic, antidyslipidemic and antioxidative effect of fixed dose combination of glibenclamide (0.6 mg/70 kg body weight) and simvastatin (5 mg/70 kg body weight) on long term alloxan induced diabetic rats with cardiovascular disease using various diagnostic kits as a parameter of phamacotherapeutic and pharmacological effect. The study was carried out using 96 Swiss Albino male rats weighing about 200–220 g. Combination therapy induced a significant decrease in blood glucose level in alloxan induced diabetic rats, from 33.75 ± 1.65 to 5.80 ± 0.07 mmol/l 2 h after last dose administration, after 4 weeks treatment. In case of dyslipidemic effect, combination therapy reduced total cholesterol (45 %), triglyceride (36 %) and low density lipoprotein-cholesterol (32 %) levels significantly and increased high density lipoprotein-cholesterol level (57 %) in comparison with their respective diabetic control groups. Results of this study showed that combination therapy effectively decreased SGPT (ALAT) (55 %) and SGOT (ASAT) (51 %) in comparison with diabetic control group. It was also observed that catalase and superoxide dismutase enzyme activity was increased by 58 and 91 % respectively in comparison with diabetic control group after 4 weeks treatment with combination of both drugs. In conclusion, these findings of combination therapy (glibenclamide and simvastatin) on alloxan induced diabetes in rats are significantly better than monotherapy using single drug. The results of the present study suggest that, combination of the fixed dose of glibenclamide and simvastatin might be efficacious in patients with diabetic dyslipidemia and increased oxidative stress. Furthermore, this combination therapy offer dosage convenience to the patients and by virtue of its dual mode of action might be a useful addition to the therapeutic armamentarium for patients with diabetic dyslipidemia and oxidative stress. 相似文献
13.
Vivek Ambade Parduman Sing B L Somani Dashrath Basanna 《Indian journal of clinical biochemistry : IJCB》2006,21(2):142-148
Albumin and enzymes-N-acetyl-beta-glucosaminidase (NAG) and gamma glutamyl transferase (GGT) were estimated in the morning
random urine samples of 196 albustix negative diabetic patients to evaluate the clinical utility of these urinary enzymes
as early markers of diabetic nephropathy. Albumin was estimated by immunoturbidimetric method and enzymes by linetic essay
within six hours of voiding of urine. The urinary albumin and urinary enzyme concentration was calculated in terms of ratio
with respect to urinary creatinine. Correlation coefficient (r) bewween urinary albumin and urinary enzymes in normoalbuminuric,
microalbuminuric and overall diabetic cases was 0.23, 0.32 and 0.40 respectively for NAG, and 0.08, 0.06 and 0.18 respectively
for GGT. NAG excretion was found increased in 34%, 63.7% and 49.5% of normoalbuminuric, microalbuminuric and overall diabetic
cases respectively while GGT in 6.4%, 24.5% and 15.8%. The correlation coefficient between urinary albumin and NAG in normoalbuminuric,
microalbuminuric, and overall diabetic patients with increased NAG excretion was found only 0.31, 0.27 and 0.35 respectively.
No correlation was found between duration of diabetes and enzyme excretion. The study suggests that urinary NAG or GGT or
both together do not have any clinical significance as an early marker of diabetic nephropathy. 相似文献
14.
15.
Valentina Vidranski Renata Laskaj Dubravka Sikiric Visnja Skerk 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2015,25(2):285-294
Background
Platelet satellitism is a phenomenon of unknown etiology of aggregating platelets around polymorphonuclear neutrophils and other blood cells which causes pseudothrombocytopenia, visible by microscopic examination of blood smears. It has been observed so far in about a hundred cases in the world.Case subject and methods
Our case involves a 73-year-old female patient with a urinary infection. Biochemical serum analysis (CRP, glucose, AST, ALT, ALP, GGT, bilirubin, sodium, potassium, chloride, urea, creatinine) and blood cell count were performed with standard methods on autoanalyzers. Serum protein fractions were examined by electrophoresis and urinalysis with standard methods on autoanalyzer together with microscopic examination of urine sediment. Erythrocyte sedimentation rate, blood culture and urine culture tests were performed with standard methods.Results
Due to typical pathological values for bacterial urinary infection, the patient was admitted to the hospital. Blood smear examination revealed phenomenon, which has persisted for three weeks after the disease has been cured. Blood smears with EDTA as an anticoagulant had platelet satellitism whereas the phenomenon was not observed in tubes with different anticoagulants (Na, Li-heparin) and capillary blood.Discussion
We hypothesize that satellitism was induced by some immunological mechanism through formation of antibodies which have mediated platelets binding to neutrophil membranes and vice versa. Unfortunately we were unable to determine the putative trigger for this phenomenon. To our knowledge this is the second case of platelet satellitism ever described in Croatia.Key words: blood platelets, thrombocytopenia, EDTA, urinary infection 相似文献16.
Indira Agarwal Chellam Kirubakaran Markandeyulu Selvakumar 《Indian journal of clinical biochemistry : IJCB》2004,19(2):45-47
Few studies have shown that calculation of protein/creatinine ratio in a spot urine sample correlates well with the 24-hour
urine collection. A study was conducted to compare the accuracy of a spot urinary protein/creatinine ratio (P/C ratio) and
urinary dipstick (albustix) with the 24-hour urine protein (24-HUP). Fifty samples from 26 patients were collected. This included
a 24-hour urine sample followed by the next voided spot sample. The protein/creatinine ratio was calculated and dipstick (albustix)
was performed on the spot sample. This was compared with the 24-hour urine protein excretion. The correlation between the
three samples was statistically highly significant (p=<0.001) for all levels of proteinuria. The normal value of protein/creatinine
ratio in Indian children was also estimated on 100 normal children attending the OPD and was calculated to be 0.053 (S.E of
mean±0.003). 相似文献
17.
Vijay Navghare Shashikant Dhawale 《Indian journal of clinical biochemistry : IJCB》2016,31(4):380-389
Musa cavendish, peels has local and traditional use to promote wound healing, hyperglycemia, ulceration etc. The present work investigated the lipid lowering; nephroprotective and glucose lowering properties of ethanolic extract of peels of Musa cavendish (EMC) in alloxan-induced diabetic rats. The EMC 250, 500 and 1000 mg/kg/day and the vehicle were administered orally to alloxan-induced diabetic rats (n = 6) for 3 weeks. Changes in plasma glucose, lipid profile along with kidney function before and after treatment with EMC were recorded. The ethanolic extract of peels of Musa cavendish reduced blood glucose, serum triglyceride, cholesterol, LDL cholesterol and creatinine levels and improvement in body weight, liver glycogen, serum HDL cholesterol, serum albumin and total protein level when compared with untreated rats. Musa cavendish has lipid lowering, nephroprotective and antidiabetic property by regulating glucose uptake in the liver and muscles by restoring the intracellular energy balance. 相似文献
18.
Halim Eshrat M. Ali Hussain 《Indian journal of clinical biochemistry : IJCB》2002,17(2):115-123
This study was carried out to investigate the effect of oral feeding of water extract of fresh leaves ofAzadirachta indica (Fam:Meliaceae) in streptozotocin induced diabetes and its associated retinopathy in rats. Treatment of the diabetic rats with aqueous extract of leaves ofA. indica at a dose of 250 mg/kg body weight for 16 weeks resulted in gradual but significant fall in blood glucose and improvement in serum total, LDL and HDL cholesterol and triacylglycerol which increased in diabetic rats. It also showed improvement in body weight and reversed retinopathy. 相似文献
19.
Prashant Kumar Rai Dolly Jaiswal Devendra K. Rai Bechan Sharma Geeta Watal 《Indian journal of clinical biochemistry : IJCB》2008,23(4):387-390
In rats with streptozotocin induced severe diabetes mellitus, aqueous extract of Trichosanthes dioica fruits at a dose of
1000mg/kg body weight daily once for 28 days reduced the levels of fasting blood glucose, postprandial glucose, asparate amino
transferase, alanine amino transferase, alkaline phosphatase, creatinine, urine sugar and urine protein where as total protein
and body weight was increased. No toxic effect was observed during LD50. Our study suggests that further detailed toxicity
studies and mechanism of action of T. dioica would be useful for undertaking human trials. 相似文献
20.
G. Sulochana S. Sethupathy L. Padmanabhan 《Indian journal of clinical biochemistry : IJCB》1989,4(1):36-40
Platelet factor 3 availability time (PF3 AT), Prothrombin time (PT), Plasma phenol, phenolic acids, blood urea and serum creatinine
were estimated in 31 uremic patients. Significant increase (P<0.01) in PF3 AT and plasma phenolic acid was seen in 100 per
cent of cases, while the increase in plasma phenol was seen in 90.3% of cases. The increase in PF3 AT was not uniformly proportional
to the increase in plasma phenol or phenolic acids in all cases. Increase in PF3 AT was significant in cases of uremia with
bleeding diathesis compared to the cases without bleeding diathesis. Increase in PF3 AT after addition of phenol and urea
together to normal platelet rich plasma (PRP) in vitro was greater than the increase in PF3 AT after the addition of phenol
or urea alone. Significant decrease (P<0.01) in PF3 AT, plasma phenol, plasma phenolic acids, blood urea and serum creatinine
was seen in uremic patients after haemodialysis indicating that the retained toxic metabolites which increase PF3 AT are dializable
substances. 相似文献