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1.
薄荷叶片愈伤组织的诱导与增殖 总被引：1，自引：0，他引：1
以薄荷(Mentha haplocalyx Briq)幼嫩叶片为外植体,采用正交设计L9(33)考察6-BA,NAA,2,4-D3种植物生长调节物质对叶片愈伤组织诱导的影响,实验结果表明薄荷叶片愈伤组织诱导最佳培养基为MS 6-BAl.0mg/L NAAl.0mg/L 2,4-D0.5mg/L 蔗糖30g/L.在此培养基上研究不同培养条件对愈伤组织增殖效应的影响,结果表明薄荷愈伤组织继代增殖适合且经济的培养条件为蔗糖20g/L,液体浅层静置培养. 相似文献
2.
珙桐愈伤组织诱导和继代培养中的褐化研究 总被引：3，自引：0，他引：3
以珙桐的叶片作外植体,对影响诱导愈伤组织的条件和基本培养基进行筛选,并对愈伤组织继代培养中的褐变问题进行研究。结果表明：WPM是珙桐叶片初代培养的最佳基本培养基;2,4-D与KT组合比NAA与6-BA组合对珙桐叶片愈伤组织诱导有更显著的影响,最佳诱导培养基组合为WPM＋2,4-D1．0mg／L＋KT2．0mg／L,继代培养中添加1．0—1．5mg／L抗坏血酸（VC）能有效抑制愈伤组织褐化。 相似文献
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红掌（Anthurium andreanum Dakota）诱导愈伤组织研究幼叶 总被引：1，自引：0，他引：1
采用红掌（Anthurium andreanum Dakota）幼叶叶片中部（带主叶脉叶片）,叶片基部（叶柄与叶片连接处）,叶尖部位作为外植体,接种在1／4MS附加不同浓度的6-BA＋2,4-D＋KT的培养基上．结果表明,红掌（Anthurium andreanum）愈伤组织的诱导率不仅与叶片不同部位有关,而且与添加外源激素的浓度及配比有关．经过60d愈伤组织诱导培养,叶片基部作为外植体诱导愈伤组织效果最好,愈伤组织诱导率最高的培养基为1／4 MS＋6-BA 0．9mg／L＋2,4-D 0．9mg／L＋KT 0．5mg／L． 相似文献
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不同植物生长调节剂对益母草愈伤组织诱导的影响 总被引：3，自引：0，他引：3
以益母草茎段、叶片两种外植体诱导产生愈伤组织．在MS培养基上,试验不同种类、不同浓度的植物生长调节剂及其组合对益母草茎段和叶片愈伤组织诱导的影响．结果表明：茎段愈伤组织诱导的较适培养基是MS附加6-BA3mg／L＋NAA1mg／L,诱导率为65％;叶片愈伤组织诱导的较适培养基是MS附加6-BA2mg／L＋NAA1mg／L,诱导率为80％．对益母草茎段和叶片外植体愈伤组织进行诱导,可为进一步研究益母草的组织培养和植株再生提供实验依据． 相似文献
6.
芦荟叶片愈伤组织诱导的研究 总被引：1，自引：0，他引：1
以中华芦荟、库拉索芦荟和木立芦荟的叶片为外植体进行组织培养。结果表明：三种芦荟均可以在特定的培养基中形成愈伤组织。中华芦荟愈伤组织的诱导最佳培养基为：MS 6-BA0.2mg／L 2，4-D0．5mg／L；库拉索芦荟愈伤组织的诱导最佳培养基为：MS 6-BA1.0mg／L 2，4-D1．0mg／L；木立芦荟愈伤组织的诱导最佳培养基为：MS 6-BA2．0mg／L 2，4-D1．5mg／L。 相似文献
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目的:建立球兰无菌苗的离体再生体系.方法:以球兰的种子为材料繁育无菌系,探讨了无菌苗叶片愈伤组织诱导情况、叶片愈伤增殖培养的最佳激素配比,比较不同培养基对丛芽诱导及对球兰生根的影响.结果:无菌苗叶片愈伤最佳激素配比为(0.5 mg/L 6-BA)+(1.0 mg/L NAA);愈伤组织的增殖最佳激素配比为(1.0 mg/L 6-BA)+(0.2 mg/L IAA);丛芽诱导最佳培养基为MS+(1.5 mg/L 6-BA)+(1.0mg/LNAA)+(6.0g/L琼脂)+(30g/L蔗糖).无菌苗生根最佳培养基为MS+(0.1mg/L NAA)+(6.0 g/L琼脂)+(30 g/L蔗糖).结论:通过筛选愈伤组织增殖、分化和丛芽诱导及促进生根的最佳培养基配方,建立了球兰的离体再生体系. 相似文献
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王廷晋 《思茅师范高等专科学校学报》2008,24(6)
以滇润楠（Machilus yunnanensis）的侧芽、顶芽为材料。在MS培养基附加细胞分裂素BA、KT和生长素NAA、2,4-D中进行培养,诱导愈伤组织以及侧芽、顶芽,结果表明,MS＋2,4-D0．5mg／l＋BA2mg／1＋AC0．3％诱导滇润楠根部萌蘖条愈伤组织的产生效果较好,MS＋2,4-D0．1mg／I＋KT2mg／1＋AC0．3％诱导滇润楠的侧芽萌动效果较好,MS＋NAA1mg／l＋BA1．5mg／l＋AC0．3％诱导滇润楠的硕芽萌动效果较好。 相似文献
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以无患子叶片为外植体,通过正交试验,研究无患子叶片愈伤组织诱导、愈伤组织诱导丛生芽、芽苗生根以及植株再生的最佳培养基组成。并对试验数据进行极差分析和方差分析。结果表明：诱导出叶片愈伤组织的最佳培养基是MS＋0．5mg·L^-1 TDZ＋0．4mg·L^-12,4-D＋6．0mg·L～AgN03,诱导率为86．4％。诱导丛生芽的最佳培养基是MS＋0．5mg·L^-1NAB．＋1．0mg·L^-1TDZ,分化率为42．5％。诱导生根的最佳培养基是1／3MS＋0．5mg·L^-16-BA＋0．5mg·L^-12,4-D,生根率为45％。 相似文献
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以丽格海棠巴科斯品系叶片为材料,进行外植体的诱导培养、不定芽继代增殖培养、无根苗生根培养等组织培养技术研究,探索其适宜的培养基和培养条件．结果表明：在MS＋BA1．0mg／L＋NAA0．2mg／L培养基上丽格海棠叶片诱导不定芽效果最好;在MS＋BA0．1mg／L＋NAA0．0lmg／L培养基上继代增殖效果较好;在1／2MS＋NAA0．2mg／L培养基生根诱导培养效果较好．苗高6cm可出瓶过渡移栽,并保持90％以上的空气湿度,温度为（25±3）℃,成活率最高． 相似文献
11.
罗艳萍 《读与写:教育教学刊》2008,5(6)
The communication of people partially is the communication of cultures. Culture has a direct effect on international commercial activities in all aspects. Different conceptions about time, space, equality, law and the like, lead people to deal with things in different ways. So to know cultures of the counterpart is to facil-itate our enterprises so as to have a smooth and successful communication in commercial activity. 相似文献
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邱崇丙 《唐山师范学院学报》2014,(1):21-23
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。 相似文献
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Richard Denner 《海外英语》2007,(4):14-15
Rosco and I wait for the fishermen to return.I sit at a wooden bench near the store at Mt.Baker Resort and watch the clouds change shape. Rosco has my belt around his neck and an eight foot tow chain hooked to a tree. Dogs must be on a leash. Ducks and rabbits are loose. 相似文献
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Given a graph G,a subgraph C is called a clique of G if C is a complete subgraph of G maximal under inclusion and |C|≥2. A clique-transversal set S of G is a set of vertices of G such that S meets all cliques of G. The clique-transversal number, denoted as TC (G), is the minimum cardinality of a clique-transversal set in G. The clique-graph of G, denoted as K (G), is the graph obtained by taking the cliques of G as vertices, and two vertices are adjacent if and only if the corresponding cliques in G have nonempty intersection. Let F be a class of graphs G such that F={G|K(G) is a tree}. In this paper the graphs in F having independent clique-transversal sets are shown and thus TC (G)/|G|≤1/2 for all G ∈ F. 相似文献
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《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help? 相似文献
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陈美曦 《山东教育学院学报》2008,23(2):103-107
Different nations have different social cultures, and the social culture of every nation is developing with the development of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in conceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language. 相似文献
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Rachel Zupek 《海外英语》2007,(11):40-41
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了 相似文献
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《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love. 相似文献