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1.
谈玲芳 《孝感职业技术学院学报》2003,6(2):77-79
目前已发现数个Bcl-2基因家族成员。主要有Bcl-2、Bax等，它们调节子宫内膜细胞的凋亡，与子宫内膜增生、癌变有密切联系。 相似文献
2.
Li Chao Brant Eleanor Budak Hikmet Zhang Baohong 《Journal of Zhejiang University. Science. B》2021,22(4):253-284
Since it was first recognized in bacteria and archaea as a mechanism for innate viral immunity in the early 2010 s,clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein(Cas) has rapidly been developed into a robust, multifunctional genome editing tool with many uses. Following the discovery of the initial CRISPR/Cas-based system, the technology has been advanced to facilitate a multitude of different functions. These include development as a base editor, prime editor, epigenetic editor, and CRISPR interference(CRISPRi) and CRISPR activator(CRISPRa) gene regulators. It can also be used for chromatin and RNA targeting and imaging. Its applications have proved revolutionary across numerous biological fields, especially in biomedical and agricultural improvement. As a diagnostic tool, CRISPR has been developed to aid the detection and screening of both human and plant diseases, and has even been applied during the current coronavirus disease 2019(COVID-19) pandemic. CRISPR/Cas is also being trialed as a new form of gene therapy for treating various human diseases, including cancers, and has aided drug development. In terms of agricultural breeding, precise targeting of biological pathways via CRISPR/Cas has been key to regulating molecular biosynthesis and allowing modification of proteins, starch, oil, and other functional components for crop improvement. Adding to this, CRISPR/Cas has been shown capable of significantly enhancing both plant tolerance to environmental stresses and overall crop yield via the targeting of various agronomically important gene regulators. Looking to the future, increasing the efficiency and precision of CRISPR/Cas delivery systems and limiting off-target activity are two major challenges for wider application of the technology. This review provides an in-depth overview of current CRISPR development, including the advantages and disadvantages of the technology,recent applications, and future considerations. 相似文献
3.
《Scientific Studies of Reading》2013,17(3):261-283
Recently a gene, termed EKN1, has been identified because of a chromosomal breakpoint that occurred in this gene. This chromosomal breakpoint was found in 4 family members that had specific reading disabilities (RDs), indicating that disruption of this gene may be contributing to the risk of developing RDs. This gene was further supported as contributing to RD by association studies. Because of the evidence from twin studies for shared genetic factors contributing to RD and attention deficit hyperactivity disorder (ADHD), particularly inattention symptoms, we investigated the relationship of DNA markers in this gene to ADHD and ADHD symptoms in a sample of 186 nuclear families (probands, their parents, and affected siblings) collected through a proband with ADHD. We used 6 polymorphic DNA markers located across the gene, including the 2 markers previously reported to be associated with RD in a Finnish sample and a marker associated with RD in a sample of families collected in Toronto. We found a trend for association for several markers to the ADHD phenotype analyzed as a categorical trait using the transmission disequilibrium test and significant evidence for biased transmission of the haplotypes containing these markers, χ2(3) = 9.312, p =. 025. Using quantitative analysis, we observed evidence for association of one of the haplotypes to the inattention and hyperactive/impulsive symptom dimensions as reported by parents and to the inattention symptoms as reported by teachers, as well as a trend for association with the reading phenotypes of word identification and decoding. The results provide preliminary support for the role of the EKN1 chromosomal region in ADHD, suggesting that this region may contribute to ADHD symptoms in addition to RD. 相似文献
4.
基于PCR技术的RGAP分子标记,由于其本身所具有的特点和优越性爱到大家的广泛关注,为近年来分子生物学领域的研究开辟了一个新途径.目前主要应用在克隆和定位抗病基因、分子标记辅助育种、系统植物学研究等方面.本文就RGAP法的基本原理、优越性、目前研究的现状、进展以及研究中还存在的问题分别作以阐述,以期使人们对此标记有个系统的认识. 相似文献
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Learning Environments Research - The learning environment has been found to be related to mathematics anxiety at a variety of educational levels, including higher education, but to date has not... 相似文献
7.
TIAN Yong LU Li-zhi FU Yan TAO Zheng-rong SHEN Jun-da WANG De-qian YUAN Ai-ping YIN Zhao-zheng 《Journal of Zhejiang University. Science. B》2007,8(5)
The protein encoded by CC chemokine receptor 7 (CCR7) is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6 000 rads chicken-hamster radiation hybrid panel (ChickRH6) was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 (6 cR away) with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH (radiation hydrid) map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene. 相似文献
8.
Scott J. Mertes 《Community College Journal of Research & Practice》2013,37(12):1170-1181
While Tinto’s (1975, 1987, 1993) Interactionalist Model has reached near paradigmatic status, and introduced the construct of social integration, the research has been inconsistent when applied to community college student retention. This has led Maxwell (2000) and Deil-Amen (2011) to suggest that a different construct of social integration exists in community colleges. This construct focuses less on purely social activities and more on both peer groups centered on academically related activities, along with interactions with faculty and students inside the classroom. While previous research found that these two constructs of social integration were highly related (Mertes, 2015), differences were found when investigating gender. But the previous research did not consider the impact of program of study and potential interactions with other variables including gender, race, and age. The current study sought to investigate the impact of program of study (i.e., occupational vs. transfer) on both constructs, as well as the impact of the interaction of program of study and additional variables including gender, race, and age. After conducting the analysis, it was found that while overall program of study did not significantly impact either construct of social integration, a significant gender difference was found on the alternative construct of social integration as suggested by Maxwell and Deil-Amen. Future research strategies including utilizing campus ecology theory are then discussed. 相似文献
9.
Scott J. Mertes 《Community College Journal of Research & Practice》2013,37(11):1052-1064
Among current retention models, Tinto’s Interactionalist Model has reached near paradigmatic status. When his model has been applied to two-year college settings, the social integration results have been inconsistent. This has led Maxwell (2000) and Deil-Amen (2011) to suggest that a different construct of social integration exists in community colleges; one that focuses less on purely social activities and more on peer groups centered on academically-related activities, and interactions with faculty and students inside the classroom. But are these two constructs really different? The current study sought to ascertain whether this alternative construct of social integration is, in fact, related to Tinto’s construct. It also was designed to investigate the impact of additional variables including gender, race, age, and program of study (i.e., occupational vs. transfer) have on both constructs. After conducting the analysis, it was found that the two social integration constructs were highly related. Additionally, it was found that social integration, using Tinto’s construct, significantly varied by gender. Future research strategies including longitudinal analysis, regression analysis, and qualitative techniques are all recommended. 相似文献
10.
采用花粉管通道法介导外源耐盐基因SeNHX1导入4个小麦品种（品系）BN-64、YM-34、BM-1和AK-58中,并对转基因D0代的主要农艺性状进行了统计分析.结果表明,与对照相比,转基因D0代植株的种子（D1代）千粒重、株高、穗长、旗叶面积均有所提高;D0代植株与对照组相比,株高差异均达到显著水平,甚至极显著水平;穗长除BM-1差异不显著外,其余受体均达显著水平.叶面积差异均达到显著水平;叶长除YM-34外,其余受体差异均达到极显著水平.叶宽与受体相比均无明显差异. 相似文献
11.
通过问卷调查、访谈、观察等方法,对甘肃某工科高校多媒体教学情况进行了实地调查。结果表明,多媒体技术的应用,为教师课堂教学提供了一个良好的教学平台,在一定程度上促进了课堂教学方式的改变与教学效率的提升,但在实际应用中也存在不少问题。为此,要树立多媒体技术教学平台的观念,树立研究性教学的观念,树立不断创新教学方法的观念;采取相应的措施加大硬件建设力度,加强对教师现代教育技术的培训,恰当地利用多媒体技术进行教学,使多媒体教学平台所拥有的传统教学方法无可替代的优势和强大的生命力得到充分的发挥。 相似文献
12.
根据已发表的序列，通过PCR技术克隆了一系列构建烟草叶绿体多顺反子表达载体所需的元件：质体核搪体（16S）RNA操纵元启动子（Prrn）、质体面A基因3’端（psbA3’）、山菠菜甜菜碱醛脱氢酶基因（BADH）、烟草叶绿体高频同源重组片段（psaA／psbC，大小3463bp）、甘露聚糖酶基因（man）、绿荧光蛋白基因（gfp）。构建了烟草质体多顺反子定点整合表达载体pLM7（-psaA-Prrn-SD-man-SD-gfp-SD-BADH-PSBA3’-PSBC-）。并在大肠杆菌中通过平板定性分析等方法对所构建载体上的表达盒进行了功能鉴定。 相似文献
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David Resnick 《Religious education (Chicago, Ill.)》2013,108(2):108-123
Rosenak has shown that contemporary Jewish education must negotiate the tension between relevance and authenticity. For those who embrace authenticity as a goal, education is often mediated through heroes--who are ideal cultural types. Such education is hampered by the diminution of heroes in contemporary culture: The hero has been replaced by the role model, and the aspiration to be like the hero has been replaced by individualism. This article explores the idea of heroes in education both in the philosophic literature (Taylor, McIntyre, Rosenak) and in various aspects of Jewish education in the United States, including a look at wider American Jewish culture in this regard. A brief comparison to Israeli Jewish education is also included, where similar tensions and dilemmas are found. 相似文献
15.
In this paper, we present a simple and fast spectra inversion method to reconstruct the temperature distribution along single
fiber Bragg grating (FBG) temperature sensor. This is a fully distributed sensing method based on the simulated annealing
evolutionary (SAE) algorithm. Several modifications are made to improve the algorithm efficiency, including choosing the most
superior chromosome, setting up the boundary of every gene according to the density of resonance peaks of the reflection spectrum,
and dynamically modifying the boundary with the algorithm running. Numerical simulation results show that both the convergence
rate and the fluctuation are significantly improved. A high spatial temperature resolution of 0.25 mm has been achieved at
the time cost of 86 s. 相似文献
16.
RNAi的研究及应用 总被引：1，自引：0，他引：1
RNA干扰（RNA interference,RNAi）是由双链RNA（double-stranded RNA,dsRNA）引发的转录后基因沉默机制．RNAi可以调节和关闭基因的表达,进而调控细胞的各种高级生命活动,是真核生物中普遍存在的抵抗病毒入侵、抑制转座子活动、调控基因表达的监控机制．目前RNAi的研究取得了很大进展,有可能为肿瘤基因治疗提供新策略． 相似文献
17.
Juan Acosta-Ballesteros Olga María Rodríguez-Rodríguez 《Journal of Education & Work》2018,31(1):28-46
The objective of this paper is to identify the effect of education on young workers’ time-related underemployment as well as analysing whether education has been a defence mechanism against the recent crisis. Especially relevant is the analysis regarding field of study, which has not been addressed in the underemployment literature. Using data from the Spanish Labour Force Survey 2006–2014, bivariate probit selection models are estimated for three different periods. This methodology enables to analyse the evolution of underemployment among young people, simultaneously considering their probability of employment. The results indicate that underemployment is negatively related to education level for each period and tertiary education has smoothed the negative effect of the recession. Moreover, those specialisations that provide students with specific and work-oriented skills (sciences, technology and health) help workers avoid underemployment and enable them to handle the recession better. Conversely, more generally oriented fields (education, and arts and humanities) are associated with a higher probability of underemployment. Similar patterns have been found regarding unemployment, indicating education plays a similar role in both working time shortages. 相似文献
18.
Assignment of <Emphasis Type="Italic">CCR7</Emphasis> gene to chicken chromosome 27 by radiation hybrid panel mapping 总被引：1，自引：0，他引：1
Tian Y Lu LZ Fu Y Tao ZR Shen JD Wang DQ Yuan AP Yin ZZ 《Journal of Zhejiang University. Science. B》2007,8(5):314-317
The protein encoded by CC chemokine receptor 7 （CCR7） is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus （EBV）, and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel （ChickRH6） was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 （6 cR away） with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH （radiation hydrid） map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene. 相似文献
19.
Xiao-Hua Jin 《Assessment & Evaluation in Higher Education》2012,37(5):577-589
In recent years, various forms of group work have been introduced in university courses across various subject domains, including construction management courses. Although the use of group work in higher education has sound pedagogical reasons and advantages, group work has its own drawbacks. Therefore, the acceptance by students and the success of group work critically depend on a fair and credible assessment of the group process. In this paper, the implementation of different approaches to peer assessment (PA) of individuals’ contributions to group projects in two core units in an undergraduate construction management course in an Australian university is reported. The effectiveness of the adopted PA approaches have been evaluated and validated by students. It has been found that contrary to doubts of the sufficiency of a simplistic approach to PAs, the fairness of a PA approach does not necessarily depend on its complexity. Besides, voluntary group discussions, learning and collaboration are found to aid in improving each of the group’s camaraderie. Hence, it is recommended that academics should develop both a structured methodology to progressively encourage group members to work cohesively in teams and effective PA approaches that measure individual member’s contribution. 相似文献
20.
大鼠左心室流出道电生理特性的观察 总被引：1，自引：1，他引：1
目的为了观察大鼠左心室流出道组织是否具有自动节律性.方法利用细胞内微电极记录技术,观察了18例大鼠左心室流出道组织的电生理特征.结果16例(88.89%)出现了自律性电活动,并且可在局部组织记录到典型的慢反应动作电位.结论大鼠左心室流出道组织具有自律性.其生理功能有待进一步研究. 相似文献