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1.
选择 9只 6 - 10月龄的健康雄性白山羊 ,经粪便检查和DotBLISA检测 ,确认无肝片吸虫感染 ,随机分成感染组 (n =5 )和对照组 (n =4 ) ,试验组每只一次口服接种 15 0个肝片吸虫囊蚴。每周定时从颈静脉采集感染前 0周和感染后 14周血液一次 ,分离血清 ,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量 ,旨在研究感染肝片吸虫对山羊机体无机盐含量的影响。结果表明感染后山羊血清中的钾、钠、钙、镁、磷、氯的含量均成波动变化 ,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化 ,这与虫体感染后机体的代谢发生改变有关。 相似文献
2.
选择9只6－10月龄的健康雄性白山羊，经粪便检查和DotBLISA检测，确认无肝片吸虫感染，随机分成感染组（n＝5）和对照组（n＝4），试验组每只一次口服接种150个肝片吸虫囊蚴。每周定时从颈静静脉集感染前0周和感染后14周血液一次，分离血清，测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量，旨在研究感染肝片吸虫对山羊机体无机盐含量的影响。结果表明感染后山羊血清中的钾、钠、钙、镁、磷、氯的含量均成波动变化，说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化，这与虫体感染后机体的代谢发生改变有关。 相似文献
3.
实验选择12头、2-3岁、体重300-500kg雄性去势水牛,经粪便检查和DotELISA检查肝片吸虫阴性,随机分成感染组(n=9)和对照组(n=3),感染组每头一次口服1600个囊蚴.每周定时分别从水牛颈静脉采集感染前0周和感染后25周血液一次,分离血清,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量,旨在研究感染肝片吸虫对水牛机体无机盐含量的影响.结果表明感染后水牛血清中的钾、钠、钙、镁、磷、氯的含量均成波状线变化,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化,这与虫体感染后机体的代谢发生改变有关. 相似文献
4.
实验选择12头、2－3岁、体重300－500kg雄性去势水牛，经粪便检查和Dot-ELISA检查肝片吸虫阴性，随机分成感染组（n＝9）和对照组(n=3），感染组每头一次口服1600个囊蚴。每周定时分别从水牛颈静脉采集感染前0周和感染后25周血液一次，分离血清，测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量，旨在研究感染肝片吸虫对水牛机体无机盐含量的影响。结果表明感染后水牛血清中的钾、钠、钙、镁、磷、氯的含量均成波状线变化。说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化，这与虫体感染后机体的代谢发生改变有关。 相似文献
5.
对陕西关中地区关中奶山羊寄生虫进行调查，解剖40只，粪便检查30只，结果发现本地区寄生虫17种，其中吸虫2种，绦虫4种，线虫9种，羊狂蝇1种及疥螨。优势虫种为肝片吸虫、矛形双腔吸虫、捻转血矛线虫、羊仰口线虫、食道口线虫、毛首线虫。并对该地区寄生虫病防治提出了科学的建议。 相似文献
6.
目的：研究人体片形吸虫病ELISA检测试剂盒的检测效果。方法：采用云南大理本地采集的牛体片形吸虫成虫制备可溶性粗抗原,包被反应板,采用人体片形吸虫病ELISA法（FAWA-ELISA）进行研究,分别检测26份片形吸虫患者血清、102份其他寄生虫病患者血清和25份健康人血清,评价检测试剂盒的检测效果。结果：检测试剂盒的敏感性为100.0%、特异性分别为95.28%（95%CI：98.9%～91.2%）,约登指数分别为0.95。结论：FAWA-ELISA试剂盒检测效果较好,且成本相对较低,可适宜用于云南片形吸虫病流行区流行病学筛查,减轻病原学检测的工作量。 相似文献
7.
目的:同时用评价试剂和参比试剂检测436例血清样本,探讨评价试剂与参比试剂检测结果的符合率.方法:评价试剂采用化学发光法,参比试剂采用双抗原夹心酶联免疫吸附试验;并通过卡方检验来判断两试剂盒间有无显著差异.结果:评价试剂和参比试剂阳性符合率为98.25%,阴性符合率为99.47%.两种试剂通过卡方检验比较差异无统计学意义(P>0.05).结论:评价试剂与参比试剂的检测结果具有很好的一致性,评价试剂可以用于梅毒感染的辅助诊断. 相似文献
8.
丙肝病毒核心抗原与丙肝病毒抗体检测的比较 总被引：1，自引：0，他引：1
目的：了解丙肝病毒核心抗原检测的意义.方法：采用HCV游离核心抗原试剂盒,对来自入院前或手术前筛查的180例临床样本和24例丙肝或疑似丙肝患者的血清样本进行HCV-cAg和HCV-Ab检测,阳性者用反转录多聚酶链反应（RT-RNA）证实.结果：180例筛查样本HCV-Ab均为阴性,HCV-cAg阳性2例,其中RT-RNA阳性1例;24例HCV-Ab阳性的样本检出HCV-cAg阳性12例,RT-RNA阳性14例.HCV-cAg与RT-RNA符合率为85.71%（12/14）.结论：HCV核心抗原检出时间早于抗体,HCV-cAg检测试剂盒可作为HCV抗体检测的补充试剂. 相似文献
9.
目的:通过检测不同浓度酒精阳性乳患牛血清中的铜、锌含量,探讨其与酒精阳性乳的关系。方法:首先剔除加州乳房炎检测阳性的奶牛,然后用68%的酒精检测酒精阳性乳,用72%的酒精测定68%的酒精阴性牛乳,再用75%的酒精测定72%的酒精阴性牛乳,每组选择产奶量、胎次、年龄、泌乳期相近的奶牛6头,组成75%酒精阴性对照组、75%酒精阳性组7,2%酒精阳性组和68%酒精阳性组。连续三周对各组奶牛进行颈静脉采血,测定血清中的铜和锌的含量。结果:各得血清铜含量差异均不显著;与75%酒精阴性对照组相比,75%酒精阳性组血清锌呈降低趋势,但各组差异不显著;72%酒精阳性组显著降低;68%酒精阳性组极显著降低。结论:酒精阳性乳的奶牛血清铜的含量与临床健康奶牛相比较无显著差异,但各组酒精阳性乳奶牛机体血锌含量降低,可能影响到机体与锌密切相关酶类的代谢。 相似文献
10.
《安徽科技学院学报》2020,(1)
目的:确定安徽及周边地区规模化山羊场阿氏艾美尔球虫感染情况。方法:从安徽及周边地区规模化羊场共采集781份山羊新鲜粪便样品,提取所有样品基因组DNA,利用阿氏艾美尔球虫ITS-1特异巢式PCR对所有样品进行检测,对阳性产物进行测序和序列分析,确定安徽及周边地区山羊阿氏艾美尔球虫感染情况。结果:安徽及周边地区山羊阿氏艾美尔球虫感染率为6.91%(54/781)。除六安外,其余采样点均发现有阿氏艾美尔球虫感染。序列分析及进化树构建显示所有的阳性样本均为阿氏艾美尔球虫。结论:尽管安徽及周边地区山羊阿氏艾美尔球虫感染率不高,但仍应引起重视。 相似文献
11.
Rajput ZI Hu SH Arijo AG Habib M Khalid M 《Journal of Zhejiang University. Science. B》2005,6(11):1057-1062
INTRODUCTION The world population of buffaloes(Bubalus bubalis)has been estimated at over172million head,more than97%(167.6million)of which are in Asia and the Pacific region,mainly in India(97.7million)and Pakistan(25.5million)(FAO,2004).About98%of buffaloes in the region are raised by small farmers owning less than two hectares of land and less than five buffaloes(Mudgal,1992).In general,the water buffalo is regarded as more productive,healthier and more useful than the cow,especiall… 相似文献
12.
本文对178例肿瘤患者和238例健康人,采用间接血凝试验(IHA)进行弓形虫抗体检测。结果患者组阳性27例,感染率为15.16％;健康人阳性者7人,感染率为2.94％。 相似文献
13.
邵洪伟 《山东教育学院学报》2009,24(4):93-95
通过长春市戊型肝炎流行病学调查,了解长春市戊肝病毒在动物与人群中的感染情况为戊肝防治提供宝贵的参考资料。对长春市采集的动物血清包括猪820份、牛506份、羊273份及人血清,包括农村饲养猪人群182份、农村非饲养猪人群102份,城镇人群546份,采用戊肝病毒抗原及抗体试剂盒检测血清中的病毒抗原及抗体。结果感染率分别为猪72.80%、牛11.86%、羊10.26%、农村饲养猪人群20.88%、农村非饲养猪人群15.38%、城镇人群4.58%。结果表明戊肝病毒在长春市的动物及人群中均有流行,且猪HEV感染比牛和羊及人都普遍,农村饲养猪人群和农村非饲养猪人群感染率都远高于普通人群,为戊肝是人畜共患病提供有力证据。 相似文献
14.
K. D. Simon Y. Bakar A. Samat C. C. Zaidi A. Aziz A. G. Mazlan 《Journal of Zhejiang University. Science. B》2009,10(12):902-911
Population growth, trophic level, and some aspects of reproductive biology of two congeneric archer fish species, Toxotes chatareus and Toxotes jaculatrix, collected from Johor coastal waters, Malaysia, were studied. Growth pattern by length-weight relationship (W=aL~b) for the sexes differed, and exhibited positive allometric growth (male, female and combined sexes of T. chatareus; female and combined sexes of T. jaculatrix) and isometric growth (male samples of T. jaculatrix only). Trophic levels of both species were analyzed based on 128 specimens. The results show that, in both species, crustaceans and insects were the most abundant prey items, and among crustaceans the red clawed crab Sesarma bidens and Formicidae family insects were the most represented taxa. The estimated mean trophic levels for T. chatareus and T. jaculatrix were 3.422±0.009 and 3.420±0.020, respectively, indicating that they are largely carnivores. Fecundity of T. chatareus ranged from 38354 to 147185 eggs for females with total length ranging from 14.5 to 22.5 cm and total body weight from 48.7 to 270.2 g, and T. jaculatrix 25 251 to 150456 eggs for females with total length ranging from 12.2 to 23.0 cm and total body weight from 25.7 to 275.0 g. Differences in values of gonadosomatic and hepatosomatic indexes calculated for both species in this study may have resulted from uneven sample size ranges. 相似文献
15.
howed true positive (TP) and true negative (77V) results from 80.5% (265 from 329) and 83.0% (415 from 500) of samples, respectively. The average computation time was 453 ms per image. 相似文献
16.
重金属及农药在孝感市食品及人体中蓄积的研究 总被引：4，自引：0，他引：4
从2000年至2003年对孝感市外环境、食品、人体生物材料共380份样品进行了重金属及六六六(BHC)、滴滴涕(DDT)蓄积量检测和分析．结果为食品中砷、铅、镉等重金属检出量在国家标准以内．菜地土残留量最高，其次是农田土、丘陵土、地表水、浅井水和深井水；食品样中，豆类、植物、油、鲜鱼、农药检出率达100%，人脂、血液中BHC检出率分别是93．33%和100%，DDT分别是100%和86．67%，人脂中BHC和DDT检出量最高达187．67μg／kg和298．47μg／kg；结果表明孝感市外环境中BHC残留量超过了绿色食品灌溉用水标准和生活饮用水卫生标准，农产品检出率为95%；提示孝感市应加大对农业环境的保护力度，严禁滥用有机氯农药，科学使用有机磷农药，对农产品基地应采取综合措施进行农药降解，以实现从土地到餐桌的食品安全． 相似文献
17.
为了解湖南地区的猪球虫感染情况,采用粪便饱和盐水漂浮法对湖南省8个地区的800份猪粪便样品进行了球虫的种类和感染情况的调查.结果表明:发现猪球虫9种,平均感染率为52.1％,其中猪艾美耳球虫(Eimeri suis)为优势种.湖南省猪球虫感染率较高,应加强猪球虫病的防制,其中猪艾美耳球虫病的预防控制的重点. 相似文献
18.
目的 :了解张家口地区精神分裂症患者弓形虫感染状况 ,探讨弓形虫感染与精神分裂症之间的关系。方法 :采用间接红细胞凝集试验对 2 2 2例住院精神分裂症患者及 78例健康体检者进行弓形虫抗体检测。结果 :张家口地区精神分裂症患者弓形虫抗体阳性率为 7.6 6 % ,健康体检者为 1.2 8% ,两者之间差异显著 (P <0 .0 5 )。结论 :本地区精神分裂症患者弓形虫抗体阳性率高于健康人群。提示临床医务工作者应加强对弓形虫病的认识 ,重视精神病患者的弓形虫感染情况 ,对门诊和住院病人进行常规弓形虫检测。 相似文献
19.
Serum protein fingerprinting coupled with artificial neural network distinguishes glioma from healthy population or brain benign tumor 总被引：6，自引：0，他引：6
To screen and evaluate protein biomarkers for the detection of gliomas (Astrocytoma grade Ⅰ-Ⅳ) from healthy individuals and gliomas from brain benign tumors by using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) coupled with an artificial neural network (ANN) algorithm. SELDI-TOF-MS protein fingerprinting of serum from 105 brain tumor patients and healthy individuals, included 28 patients with glioma (Astrocytoma Ⅰ-Ⅳ), 37 patients with brain benign tumor, and 40 age-matched healthy individuals. Two thirds of the total samples of every compared pair as training set were used to set up discriminating patterns, and one third of total samples of every compared pair as test set were used to cross-validate; simultaneously, discriminate-cluster analysis derived SPSS 10.0 software was used to compare Astrocytoma grade Ⅰ-Ⅱ with grade Ⅲ-Ⅳ ones. An accuracy of 95.7%, sensitivity of 88.9%, specificity of 100%, positive predictive value of 90% and negative predictive value of 100% were obtained in a blinded test set comparing gliomas patients with healthy individuals; an accuracy of 86.4%, sensitivity of 88.9%, specificity of 84.6%, positive predictive value of 90% and negative predictive value of 85.7% were obtained when patient's gliomas was compared with benign brain tumor. Total accuracy of 85.7%, accuracy of grade Ⅰ-Ⅱ Astrocytoma was 86.7%, accuracy ofⅢ-Ⅳ Astrocytoma was 84.6% were obtained when grade Ⅰ-Ⅱ Astrocytoma was compared with grade Ⅲ-Ⅳ ones (discriminant analysis). SELDI-TOF-MS combined with bioinformatics tools, could greatly facilitate the discovery of better biomarkers. The high sensitivity and specificity achieved by the use of selected biomarkers showed great potential application for the discrimination of gliomas patients from healthy individuals and glioma from brain benign tumors. 相似文献
20.
Objective： To detect the infection frequencies of different genotypes of Epstein-Barr virus （EBV） in subgingival samples from chronic periodontitis （CP） patients, and to discuss the correlation between infection with EBV and clinical parameters. Methods： Nested-PCR assay was used to detect EBV-1 and EBV-2 in subgingival samples from 65 CP patients, 65 gingivitis patients and 24 periodontally healthy individuals. The amplicons were further identified by restriction fragment length polymorphism analysis （RFLP） with endonucleases Afa I and Stu I. Clinical parameters mainly included bleeding on probing （BOP）, probing depth （PD）, attachment loss （AL） in six sites of the dentition. Results： In CP patients, gingivitis and periodontally healthy individuals, the infection frequencies were 47.7%, 24.6% and 16.7% for EBV-1, and 15.4%, 7.7% and 0% for EBV-2, respectively. In 2 out of the 65 CP patients co-infection of EBV-1 and EBV-2 was found. The positive rate of EBV-1 in chronic periodontitis patients was higher than that in gingivitis patients （P=-0.01） and periodontally healthy individuals （P=-0.01）. But no significant difference was shown in EBV-1 frequency between gingivitis patients and healthy individuals （P〉0.05） or in EBV-2 frequency among the three groups （P〉0.05）. In CP patients, higher mean BOP value was found in EBV-1 or EBV-2 positive patients than that in EBV negative ones （P〈0.01）, but with no statistical difference in the mean PD or AL value between EBV positive and negative patients （P〉0.05）. After initial periodontal treatment, 12 out of the 21 EBV-1 positive CP patients did not show detectable EBV-1 in subgingival samples. Conclusion： nPCR plus RFLP analysis is a sensitive, specific and stable method to detect EBV-1 and EBV-2 in subgingival samples. Subgingival infection with EBV-1 is closely associated with chronic periodontitis. Infection of EBV in subgingival samples was correlated with BOP. 相似文献