共查询到20条相似文献，搜索用时 15 毫秒
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Ioannis N. Katis Judith A. Holloway Jens Madsen Saul N. Faust Spiros D. Garbis Peter J. S. Smith David Voegeli Dan L. Bader Robert W. Eason Collin L. Sones 《Biomicrofluidics》2014,8(3)
We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper. 相似文献
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Francesca Sapuppo Andreu Llobera Florinda Schembri Marcos Intaglietta Victor J. Cadarso Maide Bucolo 《Biomicrofluidics》2010,4(2)
We describe design and miniaturization of a polymeric optical interface for flow monitoring in biomicrofluidics applications based on polydimethylsiloxane technology, providing optical transparency and compatibility with biological tissues. Design and ray tracing simulation are presented as well as device realization and optical analysis of flow dynamics in microscopic blood vessels. Optics characterization of this polymeric microinterface in dynamic experimental conditions provides a proof of concept for the application of the device to two-phase flow monitoring in both in vitro experiments and in vivo microcirculation investigations. This technology supports the study of in vitro and in vivo microfluidic systems. It yields simultaneous optical measurements, allowing for continuous monitoring of flow. This development, integrating a well-known and widely used optical flow monitoring systems, provides a disposable interface between live mammalian tissues and microfluidic devices making them accessible to detection∕processing technology, in support or replacing standard intravital microscopy. 相似文献
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Optical chromatography involves the elegant combination of opposing optical and fluid drag forces on colloidal samples within microfluidic environments to both measure analytical differences and fractionate injected samples. Particles that encounter the focused laser beam are trapped axially along the beam and are pushed upstream from the laser focal point to rest at a point where the optical and fluid forces on the particle balance. In our recent devices particles are pushed into a region of lower microfluidic flow, where they can be retained and fractionated. Because optical and fluid forces on a particle are sensitive to differences in the physical and chemical properties of a sample, separations are possible. An optical chromatography beam focused to completely fill a fluid channel is operated as an optically tunable filter for the separation of inorganic, polymeric, and biological particle samples. We demonstrate this technique coupled with an advanced microfluidic platform and show how it can be used as an effective method to fractionate particles from an injected multicomponent sample. Our advanced three-stage microfluidic design accommodates three lasers simultaneously to effectively create a sequential cascade optical chromatographic separation system. 相似文献
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We report a microfluidic blood-brain barrier model that enables both physiological shear stress and optical transparency throughout the device. Brain endothelial cells grown in an optically transparent membrane-integrated microfluidic device were able to withstand physiological fluid shear stress using a hydrophilized polytetrafluoroethylene nanoporous membrane instead of the more commonly used polyester membrane. A functional three-dimensional microfluidic co-culture model of the neurovascular unit is presented that incorporates astrocytes in a 3D hydrogel and enables physiological shear stress on the membrane-supported endothelial cell layer. 相似文献
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Xiaomei Li Jinlong Yang Kaixuan Lv Periklis Papadopoulos Jing Sun Dehui Wang Yanhua Zhao Longquan Chen Dapeng Wang Zuankai Wang Xu Deng 《国家科学评论(英文版)》2021,8(5)
Superhydrophobic surfaces are widely used in many industrial settings, and mainly consist of rough solid protrusions that entrap air to minimize the liquid/solid area. The stability of the superhydrophobic state favors relatively small spacing between protrusions. However, this in turn increases the lateral adhesion force that retards the mobility of drops. Here we propose a novel approach that optimizes both properties simultaneously. Inspired by the hydrophobic leaves of Salvinia molesta and the slippery Nepenthes pitcher plants, we designed a Salvinia-like slippery surface (SSS) consisting of protrusions with slippery heads. We demonstrate that compared to a control surface, the SSS exhibits increased stability against pressure and impact, and enhanced lateral mobility of water drops as well as reduced hydrodynamic drag. We also systematically investigate the wetting dynamics on the SSS. With its easy fabrication and enhanced performance, we envision that SSS will be useful in a variety of fields in industry. 相似文献
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根据汽车和舰船实际供电情况,文章设计了一款宽输入电压范围的稳压电源,其输入电压范围为DC4.5V-60V,输出电流达5A。整个电源方案分为稳压部分、充电管理部分和升压管理部分,分别采用TPS54560、TPS61175以及TP4056芯片。实验结果表明,该电源方案具有输入电压范围宽、转换效率高、输出稳定,可应用与车载定位模块或舰船用的定位通讯终端。 相似文献
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光电信息传感和光电能量转换是建设智慧地球和绿色地球的重要关键技术。根据当前光电信息传感器以及光电能量转换器件的发展趋势,应努力发展大规模焦平面列阵;提高红外传感器件工作温度,发展室温工作器件;扩展光电传感器工作波段,发展多波段器件;发展光、热、电、磁、分子、质量、应力以及单光子传感器等多种传感技术。本文还对如何发展太阳能光伏电池提出若干看法。 相似文献
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对锁相环型频率综合器中的高速分频器进行了较为深入的分析。比较了同步分频器和异步分频器,表明了异步分频器在高频应用中的特点。对相位切换型异步分频器中第1级和第2级2分频电路的实现方案进行了仔细分析和对比,并针对Wang提出的2分频电路中存在的电荷分享问题提出了改进方案,仿真显示改进后的电路有效解决了电路中的电荷分享问题。采用Chartered 0.35μm 2P4M CMOS工艺,对第1级与改进后的第2级2分频电路整体仿真显示,电路的最高工作频率为3.3GHz,电流消耗为1.9mA。 相似文献
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D. Schieber 《Journal of The Franklin Institute》1973,296(1):15-31
The present paper deals with the transient electromechanical interaction in a thin layer of liquid metal on sudden disruption of an imposed magnetic field. The problem is of fundamental importance for understanding the transient performance of various magnetohydrodynamic devices, such as electromagnetic agitators, pumps, liquid metal machines, etc. 相似文献
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立井防滑绳溜车保护装置采用先进技术使动力稳定可调,在滑绳、溜车状态下,即使提升机制动闸失灵,也能将钢丝绳可靠制动,而且不损绳;感力机械手依照滑绳、溜车状况,对钢丝绳进行智能抓捕;绞车房控制系统可直接监测提升机运行情况,一旦检测出滑绳、溜车状况,除声光报警外,执行机构在PLC控制下使机械手进入工作状态,完成智能抓捕动作。结构简单,动作灵活,安装方便,不需要改动原有系统,可直接安装,能够很好地与现行提升系统配合使用。此技术克服提升机的技术缺陷,完善提升机的保护设备。提高摩擦提升的可靠性,提高设备的利用效率,充分发挥提升设备的潜力,延长提升设备的使用寿命,对保证煤矿提升安全有着十分重要的现实意义。 相似文献
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针对S频段250W室外型固态功放大热耗、热流密度集中、工作环境恶劣的特点,设计了一台高效散热、耐恶劣环境、安装维护方便、外形美观的室外型机箱,并通过实际测试验证了设计的合理性. 相似文献
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《Journal of The Franklin Institute》2003,340(3-4):283-292
The work of Lucy Suchman produced a paradigmatic shift in the cognitive science community by creating an awareness of the role of context in human cognition. Prior to that, cognitive scientists attempting to create interactive computer systems1 believed that if the system had a good likeness of its user's knowledge of the world it could understand and therefore successfully respond to its user's needs. As a result, cognitive scientists attempted to supply to computer systems the knowledge, or plans, that users brought to bear when solving problems so that the computer systems could examine that knowledge and predict what users needed to know.Suchman argued that problem-solving did not proceed simply by applying plans of action. Rather, she showed that plans were but one of the resources that people use in problem-solving: plans help people decide what features of the environment to attend to in order to attain their goals.Suchman then extended this insight in a way that created a new field of research. She took the position that interactive systems need to match the context-driven way in which people use technologies in their work environments. As a result, she developed the methodology of ethnographically based design. In this method, working prototypes are developed and designers follow users to see what their work and their work contexts are like, as users try out the prototypes in their daily work environments.Using this approach, Suchman uncovered previously unnoticed creative and improvisational abilities common in the workplace. Further, she suggested systems that would enhance, rather than quash, these ways of working.Suchman's approach, now used in academia and industry both in the US and throughout Europe, has impacted significantly on the design of computer systems in a wide range of domains such as space exploration, medical monitoring of elders living on their own, and large-scale document retrieval. 相似文献
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随着社会生产力的发展和广大群众物质文化生活水平的提高,人们对改善生活和工作的环境有了新的要求,尤其对与个人生活息息相关的家居小环境的装修和美化,已成为当今社会潮流中的一个热点——家庭装修热。室内装饰不仅在城市蓬勃发展为热门行业,而且正向农村延伸。室内装饰的艺术设计主要任务是对人们生活、工作环境空间进行美化的艺术设计．使... 相似文献
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人工神经网络在电力系统谐波分析中的应用 总被引：1，自引：0，他引：1
电力系统中各种非线性负载的广泛应用,特别是各种电力电子装置的广泛应用,是系统产生谐波的主要根源;而大量的电力电容器组的使用,则进一步加剧了谐波的危害。本文主要围绕如何用Adaline神经元模型实现电力系统的谐波分析,并分析了Adaline神经元模型的改进方法。 相似文献
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This paper deals with the experimental aspects of ultrasonic delay lines in liquid media and is a sequel to a previous paper treating the general theory of such devices.Delay devices employing ultrasonic propagation through liquids have proved satisfactory for delay times of the order of 3 milliseconds or less. Such devices can be made broadband and give excellent reproduction of pulse shape. The design considerations for such devices are discussed and illustrated by actual examples. 相似文献
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李宏 《科技成果管理与研究》2011,(5):63-65
针对原有分相式的阀基电子设备进行了新的研究,将三相的阀基电子设备集中为两个单元,分别为光电触发单元和阀监控单元,介绍了新的阀基电子设备的结构、工作原理,分析了改造后的效果。 相似文献
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空间光学遥感器的计算机辅助热分析方法 总被引：4，自引：0，他引：4
介绍了一套空间光学遥感器的计算机辅助热分析方法 ,包括热平衡计算、热弹性分析、热光学分析、热光学灵敏度分析以及热设计方案评价等。热平衡计算用来预示遥感器的温度分布 ,热弹性分析用来计算热致变形和面型变化 ,热光学分析讨论热致位移和面型变化对成像质量的影响 ,热光学灵敏度分析则讨论不同形式的温差变化对成像质量影响的敏感程度。通过上述分析 ,能够弄清空间热环境对遥感器温度分布以及温度分布对成像质量的影响 ,进而提出热控制指标、制定合理的热设计方案。在工程任务的实际应用中表明该方法是可行的 相似文献