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1.
真菌木聚糖酶生产和应用研究所   总被引:1,自引:0,他引:1  
黑曲霉(ASPERGILLUSNICER)菌株固态发酵的产酶过程研究表明,培养3天产木聚糖酶活力达最高,SephadexG-100,DEAE-SephadexA-50。经分离纯化后获得三个组分,X1X2,X3,它们的最适反应温度和pH分别为40,50,50℃和3.5,4.5,5.0.木聚糖酶相关性质和应用也进行了研究。  相似文献   

2.
通过筛选从土壤和腐朽树木中得到了4株纤维素酶高产菌株MF1、MF4、TF3和TF9,经初步鉴定分别是绿色木霉、青霉属、康氏木霉和黑曲霉.通过对TF3发酵产酶的影响因素研究表明,其发酵产酶的最佳碳源是麸皮-纤维素粉复合物,最佳氮源是硫酸铵,最适发酵温度范围是30~32℃,最适初始pH范围是6~7.  相似文献   

3.
本文研究了青霉菌(Penicilliumsp.)P1固态发酵培养的产酶过程,发酵4天产木聚精酶活力最高,固体曲最适浸提比为1:7(w/v),通过65%饱和度的硫酸按盐析,获得的木聚糖酶效果较好。40℃烘干酶粉活力为1305.6IU/g,得率为69.5%。酶反应最适pH4.6,最适温度50℃。酶与底物半纤维素作用的亲合性大小顺序为:麸皮>稻草>蔗粉>玉米芯。  相似文献   

4.
从商丘市不同环境的土壤中分离筛选到几株产脂肪酶的霉菌菌株,作者对其中的一株根霉(Rhizopus delemar)进行了液体发酵的研究,使用发酵产酶培养基组成(%):豆粉6.0,蛋白胨4.0,蔗糖1.0,K2HSO4 0.2,MgSO4·7H2O0.1,pH6.5.产酶最适温度28 ℃,接种量为1 ml,培养周期48 h,转速200 r/min.  相似文献   

5.
β—葡萄糖苷酶产生菌及其发酵培养基的优化   总被引:2,自引:0,他引:2  
通过不同菌株筛选,获得了一株高产β-葡萄糖苷酶菌株米曲霉F97。经过在子及正交试验。确立了产酶的最适培养基配方,麸皮4%,KH2PO40.1%,蛋白胨0.1%,水所苷0.05%,并按所优化的发酵培养基,发酵液最终酶活达184.18u/mL。  相似文献   

6.
通过测定几丁质酶活力的方法对来源于不同寄主和采集地的16株玫烟色棒束孢进行筛选,获得活性较高菌株RCEF3304,并对高活力菌株RCEF3304产几丁质酶的pH值、装液量、接种量和温度等发酵条件进行优化研究。结果表明高酶活菌株产酶最适条件:胶体几丁质2%,NaNO33%,Mg2+0.05%,培养基初始pH值6.0,接种量12%,100 mL装液量为10 mL,温度25℃和培养时间36 h;最适发酵工艺条件下,几丁质酶酶活OD值达到0.486,比优化前发酵水平提高了128%。  相似文献   

7.
通过筛选从土壤和腐朽树木中得到了4株纤维素酶高产菌株MF1、MF4、TF3和TF9,经初步鉴定分别是绿色木霉、青霉属、康氏木霉和黑曲霉。通过对TF3发酵产酶的影响因素研究表明,其发酵产酶的最佳碳源是麸皮-纤维素粉复合物,最佳氮源是硫酸铵,最适发酵温度范围是30~32℃,最适初始pH范围是6~7。  相似文献   

8.
通过筛选从土壤和腐朽树木中得到了4株纤维素酶高产菌株MF1、MF4、TF3和TF9,经初步鉴定分别是绿色木霉、扩展青霉、康氏木霉和黑曲霉.通过对TF3发酵产酶的影响因素研究表明,其发酵产酶的最佳碳源是麸皮-纤维素粉复合物,最佳氮源是硫酸铵,最适发酵温度范围是30~32℃,最适宜初始pH范围是6~7.  相似文献   

9.
采用单因素及均匀设计法对产几丁质酶Bt-016菌株的发酵培养基和发酵条件进行优化。单因素试验结果确定了几丁质、氮源、碳源、pH和接种量为影响发酵条件的显著影响因子,采用U9(95)水平对5个影响因子进行最佳发酵条件和培养基优化,结果表明:蔗糖2g/L、蛋白胨20g/L、初始pH值为8、接种量1. 572%、几丁质含量2. 5%为最适发酵培养条件,在此条件下,Bt-016发酵液OD600值为3. 864 5,较优化前提高了21%;产几丁质酶活力为2. 796 1 U/m L,较优化前提高了33%。研究结果为杀虫防病Bt菌株的开发提供了菌源依据。  相似文献   

10.
黑曲霉A3木聚糖酶固态变温发酵及曲盘试验研究   总被引:2,自引:0,他引:2  
黑曲霉A3是一株产木聚糖酶活力较高的菌株。本文研究了其在变温条件下的固态发酵产木聚糖酶情况,得出该菌利用麦秸、玉米芯和麸皮为原料.从33℃培养24小时后变温到28℃,可使酶活达到7200IU/g干培养基,产酶期比28℃恒温培养提前14小时,并在此基础上进行了曲盘扩大培养,酶活达到5118IU/g干培养基.  相似文献   

11.
《海外英语》2007,(5):44-45
It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,  相似文献   

12.
一、吃和喝吃苹果 eat an apple, 吃药 take medicine,吃糖 have some sweets,吃饭 have one's meals,吃馆子 dine out,吃惊 be surprised/  相似文献   

13.
《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help?  相似文献   

14.
汉字倒说     
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。  相似文献   

15.
《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love.  相似文献   

16.
王菲 《华章》2007,(12):273-273
Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject.  相似文献   

17.
裴水妹 《华章》2007,(11):196
Sister Carrie is one of the most controversial characters in American literature.Thought as a "fallen woman" firstly,she was defined as a "new woman" by some critics later. However, by digging into the motivaton behind the whole process of Carrie's "success", the relationship between Carrie and her creator (the author), the social conditions of then American, it can be found that Carrie has never been free-standing on her thought and she has never found her real-sdf even after becoming a famous actress. In a society dominated by mass consumerism Carrie is only an adherent of her own desires. She also is a representative of all those country girls flooded into cities, a symbol and a sacrifice of the urbanization of America in a time countryside was overcome by cities.  相似文献   

18.
19.
1.IntroductionOne-cyclecontrolmethod,whichwasproposedaboutonedecadeago[1],hasbecomeanattractivemethodinspecialfieldssuchaspowerfactorcorrection[2-6],switchingamplifiers[7,8],etc.Themainideaofthiscontrollerisbasedonintegrationofdiodevoltageinone-cycleandforcesittobeexactlyequaltothereferencevalue.Themainadvantageofthiscontrollerisitsrealtimeabilitytorejectthevariationofinputvoltage[1].Despitethisgreatability,ithasnogoodperformancesinrejectingofloaddisturbanceandfollowingreferencecommands.Espec…  相似文献   

20.
钱生钱!     
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了  相似文献   

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