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目的：用高效液相色谱法测定小檗碱甲氧苄啶胶囊的含量。方法：用十八烷基硅烷键合硅胶为填充剂;以0.05mol/L磷酸二氢钾溶液-乙腈-三乙胺（70：30：0.1）为流动相;检测波长为263nm;流速为1.3ml/min。结果：该方法在0.008mg/ml～0.08mg/ml范围内,小檗碱峰面积与质量浓度呈良好的线性关系。线性方程为y=3.106E-08x＋4.980E-05,线性相关系数r=0.9999,回收率为99.8%,重复性实验RSD=0.31%（n=6）。结论：方法简便,快速,准确,适用于该产品质量控制。 相似文献
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脱水苦瓜护绿和着色方法的研究和比较 总被引：5，自引：0，他引：5
研究了苦瓜在加工过程中采用的护绿、着色方法及其效果，确定其工艺条件，结果表明，0．5cm厚苦瓜片，经0．20mol/L的Na2CO3浸泡1min后，于85－90℃水中烫漂2．5 min，充分快速冷却后，浸泡于200mg/L的ZnAc O.3?Cl2 800mg/L EDTA护绿液中或置于200mg/L的叶绿素铜钠盐溶液中，液压处理10－20min，沥干后先天85℃烘2h，再于50℃烘3h，成品色泽理想，同时对叶绿素铜钠着色和护绿的色泽效果进行了比较。 相似文献
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文章探讨了CASS工艺在生活污水处理及回用中的应用.运行结果表明,进水CODcr为150-300mg/L,BOD5为80-210mg/L时,SS为100-220mg/L时,出水CODcr≤50mg/L,BOD5≤10mg/L时,SS≤10mg/L,完全符合回用要求. 相似文献
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针对水源水中硝酸盐氮（NO3-N）,采用批实验开展了生物脱氮研究,考察了碳源种类对生物脱氮的影响,并考察了豌豆秆作为固体碳源时颗粒铁、碳源用量、N03-N初始浓度和水温等因素对生物脱氮的影响。实验结果表明：甲醇、豌豆秆和木屑完全脱氮的时间分别为2天、7天和30天;三种碳源均会引起亚硝酸盐氮的短暂积累,但最大积累量出现的时间不同;在模拟水中富含溶解氧时,含有颗粒铁的反应瓶在2天内可以实现完全脱氮,而对照瓶仅去除45．7％的N03-N;第2天时,1g豌豆秆时N03-N去除率为47．8名,3g时去除率为71．9％,而5g时去除率为99．6％;低浓度和高浓度时的豌豆秆生物脱氮速率分别是11．28mg／L／d和10．91mg／L／d;15。C时的豌豆秆生物脱氮速率约为33。c时的1／3,27cc时的脱氮速率约为33oc时的3／4。碳源种类、颗粒铁、豌豆秆用量和水温显著影响豌豆秆生物脱氮效果;在既定浓度范围内（5．65mg／L-21．92mg／L）,N03-N初始浓度对生物脱氮基本无影响。 相似文献
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BackgroundPlastic waste is a serious problem because it is difficult to degrade, thereby leading to global environment problems. Poly(lactic acid) (PLA) is a biodegradable aliphatic polyester derived from renewable resources, and it can be degraded by various enzymes produced by microorganisms. This study focused on the scale-up and evaluated the bioprocess of PLA degradation by a crude microbial enzyme produced by Actinomadura keratinilytica strain T16-1 in a 5 L stirred tank bioreactor.ResultsPLA degradation after 72 h in a 5 L bioreactor by using the enzyme of the strain T16-1 under controlled pH conditions resulted in lactic acid titers (mg/L) of 16,651 mg/L and a conversion efficiency of 89% at a controlled pH of 8.0. However, the PLA degradation process inadvertently produced lactic acid as a potential inhibitor, as shown in our experiments at various concentrations of lactic acid. Therefore, the dialysis method was performed to reduce the concentration of lactic acid. The experiment with a dialysis bag achieved PLA degradation by weight loss of 99.93%, whereas the one without dialysis achieved a degradation of less than approximately 14.75%. Therefore, the dialysis method was applied to degrade a commercial PLA material (tray) with a conversion efficiency of 32%, which was 6-fold more than that without dialysis.ConclusionsThis is the first report demonstrating the scale-up of PLA degradation in a 5 L bioreactor and evaluating a potential method for enhancing PLA degradation efficiency.How to cite: Panyachanakul T, Sorachart B, Lumyong S, et al. Development of biodegradation process for Poly(DL-lactic acid) degradation by crude enzyme produced by Actinomadura keratinilytica strain T16-1. Electron J Biotechnol 2019;40. https://doi.org/10.1016/j.ejbt.2019.04.005 相似文献
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目的：用高效液相色谱法测定甲硝唑呋喃唑酮栓的含量。方法：用十八烷基硅烷键合硅胶为填充剂;以甲醇－水（20：80）为流动相：检测波长为315nm;流速为1．0ml／min。结果：该方法在0．01033mg／ml～0．05165mg／mlmg／ml范围内,甲硝唑峰面积与质量浓度呈良好的线性关系。线性方程为Y=3．529E－5X＋4．569E－7,线性相关系数r=0．9999,在0．01073mg／ml～0．05365mg／ml范围内,呋喃唑酮峰面积与质量浓度呈良好的线性关系。线性方程为Y=2．563E－5X＋3．563E－7,线性相关系数r=0．9999,回收率为99．5％和99．9％,重复性实验RSD=0．32％、RSD=0．22％（n=5）。结论：方法简便,快速,准确,适用于该产品质量控制。 相似文献
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灌溉水矿化度对玛纳斯流域棉花生长影响的试验研究 总被引：4，自引：0，他引：4
合理利用咸水资源对缓解干旱区绿洲农业用水矛盾有重要意义。本文于2010年和2011年在石河子大学节水灌溉试验站研究了矿化度为0.87、2、3、4、6、8g/L的咸水对土壤水盐动态和棉花生长及产量的影响。结果表明,咸水灌溉导致土壤剖面持续积盐,棉花叶面积指数减小,干物质积累减少、产量下降;灌溉水矿化度每提高1g/L,产量降低约391kg/hm2;土壤根系层(0～60cm)可溶性盐浓度每升高1g/L,产量降低约861kg/hm2。在灌溉水矿化度2g/L情况下,100cm土层盐分最大累积率2010年为113%,2011年为26%,呈下降趋势,棉花生长、干物质积累及产量受影响较小,可作为该区咸水利用调控参考阈值,指导农业生产。 相似文献
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BackgroundCurrently, microbial fermentation method has become the research hotspot for acetoin production. In our previous work, an acetoin-producing strain, Bacillus subtilis SF4-3, was isolated from Japanese traditional fermented food natto. However, its conversion of glucose to acetoin was relatively low. In order to achieve a high-efficient accumulation of acetoin in B. subtilis SF4-3, main medium components and fermentation conditions were evaluated in this work.ResultsThe by-products analysis showed that there existed reversible transformation between acetoin and 2,3-butanediol that was strictly responsible for acetoin production in B. subtilis SF4-3. The carbon sources, nitrogen sources and agitation speed were determined to play crucial role in the acetoin production. The optimal media (glucose·H2O 150 g/L, yeast extract 10 g/L, corn steep dry 5 g/L, urea 2 g/L, K2HPO4 0.5 g/L, MgSO4 0.5 g/L) were obtained. Furthermore, the low agitation speed of 300 r/min was found to be beneficial to the reversible transformation of 2,3-butanediol for acetoin production in B. subtilis SF4-3. Eventually, 48.9 g/L of acetoin and 5.5 g/L of 2,3-butanediol were obtained in a 5-L fermenter, and the specific production of acetoin was 39.12% (g/g), which accounted for 79.90% of the theoretical conversion.ConclusionsThe results indicated acetoin production of B. subtilis SF4-3 was closely related to the medium components and dissolved oxygen concentrations. It also provided a method for acetoin production via the reversible transformation of acetoin and 2,3-butanediol. 相似文献
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Prerna K. Chawla Alpa J. Dherai Tester F. Ashavaid 《Indian journal of clinical biochemistry : IJCB》2016,31(2):209-214
Voriconazole, an antifungal drug exhibiting wide inter-individual variability, is an ideal candidate for therapeutic drug monitoring (TDM). The aim of the present study was to standardize a simple, sensitive and rapid high performance liquid chromatography (HPLC) method with ultraviolet detection to determine plasma voriconazole concentration. The HPLC method consisted of a combination of acetonitrile and water (7:3) as mobile phase with 1 ml/min flow rate and detection at 255 nm. Plasma protein precipitation was carried out using perchloric acid and the filtered supernatant was passed through C18 column (250 × 4.6 mm, 5 μm) for the separation of voriconazole. The limit of quantification of voriconazole was 0.2 mg/L. The assay was validated with a linearity of 0.2–15 mg/L and used clinically for TDM in patient samples. The inter-assay precision was below 15 % for routine quality control samples. Weight based voriconazole doses were prescribed to 26 patients for empirical treatment of invasive fungal infections. Voriconazole therapy was managed from the baseline drug levels and follow up analysis reflected achievement in clinical efficacy. Routine TDM of voriconazole may reduce adverse events and improve the treatment response in invasive fungal infections. 相似文献
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用单扫示波极谱法，青藤碱在0．1mol／LNa_2B_4O_7中有两个还原峰，峰P_1和P_2的电位分别为-1．45V和-1．67V（vs．SCE），峰P_1的峰电流与浓度在0．06～1．8mg／L和20～34．6mg／L范围内有线性关系，检测限为0.02mg／L，用以测定中药膏风藤克总生物碱的含量，结果令人满意．实验证明，青藤碱的电极反应过程为不可逆的四电子过程，并讨论了青藤碱对超氧阴离子自由基（O_2~-）的清除作用。 相似文献
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为研究利用氧化剂快速控制活性污泥工艺中的丝状菌污泥膨胀问题,本试验以啤酒废水作为底物,利用SBR进行投加氯气来控制丝状菌污泥膨胀的研究。结果表明,氯气在膨胀控制期间投加量宜控制在30mg/L以内,运行7周期膨胀得到控制。此时污泥降解底物能力有所下降,但出水水质并未恶化,出水COD,氨氮和总磷分别为78.3mg/L、3.15 mg/L、0.38 mg/L。pH值是影响氯气氧化作用的重要因素,SVI随环境pH值的增大缓慢上升;运行至第9周期,COD去除率随pH值的增大快速上升。 相似文献
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利用盐酸多巴酚丁胺对血红蛋白模拟酶催化L-酪氨酸和H2O2体系的抑制作用,建立了酶催化荧光法测定盐酸多巴酚丁胺的新方法。研究了该猝灭反应的最佳实验条件,其猝灭程度与盐酸多巴酚丁胺的浓度呈线性关系。测定的线性范围为5.92×10-7～8.88×10-6mol/L,检出限为1.43×10-9mol/L。对浓度为5.92×10-7mol/L的盐酸多巴酚丁胺溶液进行11次平行测定,其相对标准偏差为3.2%。该方法用于药物中盐酸多巴酚丁胺含量的测定,结果满意。 相似文献
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挑选47组常用的Skyrme参数，采用Skyrme能量密度泛函结合拓展的Thomas－Fermi展开，用约束的密度变分法，我们计算了208Pb、112~124Sn 、 48Ca 、 96Zr、18O 等丰中子核的中子皮厚度并与实验数据分析抽取的中子皮厚度比较，研究了中子皮厚度与非对称核物质态方程中同位旋相关的对称能项之间的关系。我们发现中子皮厚度随对称能系数 a s 以及对称能在饱和密度附近的斜率L、曲率Ksym的增大呈现近似的线性增大的趋势，对称能越硬的核势确定的中子皮越厚。以反质子湮灭法拟合的参考实验值作为标准，我们给出对称能系数，对称能在饱和密度附近的斜率和曲率的参考范围分别为：as = 30 ~ 35MeV ，L = 20 ~ 100MeV，Ksym = -256 ~ 70MeV。满足这个条件的Skyrme 参数有：SLy0-SLy10、SkM*、SkP、SLy230a、SkMP、SKT1-3、SkI3、MSk1-2、SII 。 相似文献