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1.
Nilima N. Dongre Adinath N. Suryakar Arun J. Patil Jeevan G. Ambekar Dileep B. Rathi 《Indian journal of clinical biochemistry : IJCB》2011,26(4):400-406
The purpose of this study was to find out the effect of lead exposure on systolic and diastolic blood pressure, heme biosynthesis
related and hematological parameters of automobile workers. For this study 30 automobile workers were selected and compared
with 30 age matched healthy control subjects. Significantly increased blood lead (364%, P < 0.001) and urinary lead (176%, P < 0.001) levels were observed in automobile workers (study group) as compared to controls. Systolic blood pressure (5.32%,
P < 0.05) and diastolic blood pressure (5.87%, P < 0.05) were significantly increased in the automobile workers as compared to controls. The significantly decreased non-activated
erythrocyte δ-aminolevulinic acid dehydratase (δ-ALAD) (−18.51%, P < 0.01) and activated δ-ALAD (−13.29%, P < 0.05) levels were observed in automobile workers as compared to normal healthy control subjects. But the ratio of activated/non-activated
δ-ALAD was significantly increased (43.83%, P < 0.001) in automobile workers as compared to controls. Excretions of δ-aminolevulinic acid (83.78%, P < 0.001) and porphobilinogen (37%, P < 0.001) in urine were significantly increased in the study group as compared to the controls. In automobile workers heamoglobin
(−11.51%, P < 0.001), hematocrit (−4.06%, P < 0.05), mean corpuscle volume (−3.34%, P < 0.05), mean corpuscle hemoglobin (−5.66%, P < 0.01), mean corpuscle hemoglobin concentration (−7.67%, P < 0.001), red blood cell count (−14.6%, P < 0.001) were significantly decreased and total white blood cell count (11.44%, P < 0.05) increased as compared to the controls. The results of this study clearly indicate that the absorption of lead is
more in automobile workers and it affects on blood pressure, heme biosynthesis and hematological parameters observed in this
study group. 相似文献
2.
Raviraja A Vishal Babu GN Sehgal A Saper RB Jayawardene I Amarasiriwardena CJ Venkatesh T 《Indian journal of clinical biochemistry : IJCB》2010,25(3):326-329
Ayurveda is a traditional form of medicine used by majority of the Indians. Here we report three cases of lead toxicity, following
intake of Ayurvedic medicines. Three patients presented with blood lead levels (BLLs) of 122.4, 115 and 42.8 μg/dl respectively
at the time of hospitalization. The first case was chelated with D- penicillamine, the second with calcium disodium ethylene
diamino tetra acetate (EDTA) and the third with environmental intervention and education. Associated Ayurvedic products were
collected from patients and analyzed for metallic concentration. Cessation of Ayurvedic medication along with chelation, nutritional
intervention and education, reduced the BLL to 27.4 μg/dl in the first case after 1 year, 21.1 μg/dl after 9 months in the
second and 18.2 μg/dl after 6 months in the third case. 相似文献
3.
Gacche RN Shaikh RU Chapole SM Jadhav AD Jadhav SG 《Indian journal of clinical biochemistry : IJCB》2011,26(3):303-308
The study was designed to evaluate the antioxidant activity and effect of Cymbopogon martinii (Roxb.) Wats. (Poaceae) leaves on the activity of monoamine oxidase and kinetics of enzyme inhibition. Ethanol extract of
C. martinii and rat brain mitochondrial monoamine oxidase preparation ware used to study the kinetics of enzyme inhibition using double
reciprocal Lineweaver–Burk plot. The DPPH was used as a source of free radical to evaluate antioxidant potential. It is observed
that, the ethanolic extract of C. martinii inhibits the monoamine oxidase activity with competitive mode of inhibition. The V
max (0.01 mM/min) remained constant while, K
m varied from 21.00 ± 1.1, 43.33 ± 1.5 and 83.33 ± 1.4 mM for 100–500 μg/ml concentration of C. martinii. The K
i values were calculated to be 90.00 ± 0.87, 75.00 ± 0.69, 68.18 ± 0.68 μg for 100–500 μg/ml concentration of C. martini. It also shows a significant DPPH (1,1-diphenyl-2-picryl hydrazine) radical scavenging (IC50 = 0.34 ± 0.05 mg/ml) and reducing activity (IC50 = 0.70 ± 0.22 mg/ml). The C. martini can be considered as a possible source of MAO inhibitor used in the treatment of depression and other neurological disorders. 相似文献
4.
Amit Kumar Mani Tiwari Abbas Ali Mahdi Fatima Zahra Sudarshna Sharma Mahendra Pal Singh Negi 《Indian journal of clinical biochemistry : IJCB》2012,27(3):246-252
To correlate blood lead levels (BLLs) and oxidative stress parameters in pregnant anemic women. A total of 175 pregnant women were found suitable and included for this study. Following WHO criteria, 50 each were identified as non-anemic, mild anemic and moderate anemic and 25 were severe anemic. The age of all study subjects ranged from 24–41 years. At admission, BLLs and oxidative stress parameters were estimated as per standard protocols and subjected with ANOVA, Pearson correlation analysis and cluster analysis. Results showed significantly (p < 0.01) high BLLs, zinc protoporphyrin (ZPP), oxidized glutathione (GSSG), lipid peroxide (LPO) levels while low delta aminolevulinic acid dehydratase (δ-ALAD), iron (Fe), selenium (Se), zinc (Zn), haemoglobin (Hb), haematocrit (Hct), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC), red blood cell (RBC) count, reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (TAC) in all groups of anemic pregnant women as compared with non anemic pregnant women. In all groups of pregnant women, BLLs showed significant (p < 0.01) and direct association with ZPP, GSSG and LPO while inverse relation with δ-ALAD, Fe, Se, Zn, Hb, Hct, MCV, MCH, MCHC, RBC, GSH, SOD, CAT and TAC. Study concluded that low BLLs perturb oxidant-antioxidant balance and negatively affected hematological parameters which may eventually Pb to Fe deficiency anemia during pregnancy. 相似文献
5.
Suneesh Kumar Pachathundikandi Earaly Thomas Varghese 《Indian journal of clinical biochemistry : IJCB》2006,21(2):114-117
Blood zinc protoporphyrin (ZPP), serum total protein (TP), and total cholesterol (TC) levels in automobile workshop workers
in relation to lead toxicity were analysed. In the present study, automobile workshop workers (healthy male workers at an
age between 28 and 35 from four major automobile workshops in Kottayam, Kerala State, India) and the control (male healthy
adults at an age between 28 and 35 residing at Aymanam, a distant village at Kottayam District, Kerala having reduced or no
chance of lead exposure) displayed significant difference in blood lead (BPb) and blood ZZP (BZPP) level. The mean value of
BPb in automobile workshop workers was 15.76±0.33 μg/dl, while in the control it was 8.20±0.15 μg/dl. In automobile workshop
workers, the mean value of BZPP was 34.2±0.62 μg/dl. The control group exhibited a mean of 11.5±0.22 μg/dl. Automobile workshop
workers exhibited significant increase in BZPP was corresponding to the increase in BPb level. The total protein levels estimated
in automobile workshop workers showed significant decrease compared to control individuals, but was within the reference range
of healthy individuals. The mean value of TP level in automobile workshop workers and control was 6.9±0.13 g/dl and 7.71±0.18
g/dl, respectively. There was no significant difference in blood haemoglobin (BHb) level among the automobile workshop workers
and control. The serum TC level in automobile workshop workers showed significant decrease compared to the control individuals,
but was with in the reference range of healthy individuals. The mean level of serum TC in automobile workshop workers was
162.00±3.44 mg/dl and the same in control was 172.86±4.32 mg/dl. The present study affirms occupational lead toxicity in automobile
workshop workers and its effect on serum protein and cholesterol levels. 相似文献
6.
Shailja Chambial Kamla Kant Shukla Shailendra Dwivedi Pankaj Bhardwaj Praveen Sharma 《Indian journal of clinical biochemistry : IJCB》2015,30(3):357-359
Lead interferes with a variety of body processes and is toxic to many organs and tissues including the heart, bones, intestines, kidneys, and reproductive and nervous systems. Routes of exposure to lead include contaminated air, water, soil, food, and consumer products. The possibility of lead exposure in humans is therefore of great significance from health point of view. Occupational exposure is a common known cause of lead poisoning in adults but current status of adults exposed otherwise is not known. School teachers representing wide local population were selected and asked to furnish information regarding possible lead exposure. Blood lead level (BLL) was estimated using anodic stripping voltammetry. The mean BLL was 6.89 ± 9.5 μg/dl (<3.5–>65 μg/dl) in representative adult population. Out of the total 16 % were found to be having BLL >10 μg/dl which has significantly decreased from leaded gasoline era. Those with increased BLL (>10 μg/dl) were found to have common determinants like usage of old metallic pipes for plumbing, water consumption without any purification system, usage of cosmetics and Ayurvedic/herbal medicines. 相似文献
7.
Madhusudhana Rao A Anand U Anand CV 《Indian journal of clinical biochemistry : IJCB》2011,26(1):28-31
Numerous lines of evidence implicate a role of myeloperoxidase (MPO) in the pathogenesis of cardiovascular disease (CVD).
It is a well accepted fact that patients with chronic kidney disease (CKD) are at an increased risk for CVD. MPO is a pro-oxidant
enzyme which could be involved in the increased susceptibility of these patients to CVD. Hence, the levels of plasma MPO was
determined in healthy controls as well as in patients with CKD [stratified with the level of their kidney failure as CKD stages
II–V (end stage renal disease)]. Plasma MPO was assayed by a spectrophotometric method. Serum urea and creatinine were estimated
on a clinical chemistry analyzer using standard laboratory procedures. The mean plasma MPO levels were significantly lower
with advancing stages of renal failure (P < 0.001). There was a positive correlation between MPO and GFR (r = +0.89, P < 0.001) and a negative correlation with urea (r = −0.85, P < 0.001) and creatinine (r = −0.82, P < 0.001). While an inverse association was observed between plasma MPO and urea in CKD patients, such an association was
not observed in control subjects (P = 0.43). In conclusion, the decline in plasma MPO levels may be due to the inhibitory effect of uraemic toxins on the enzyme. 相似文献
8.
Lead poisoning is well documented in persons occupationally exposed to lead. What is less known is, that even in persons working
in lead based industries, the effect of lead and the appearance of signs and symptoms of lead poisoning is genetically determined.
Three genes related to lead metabolism, exhibiting polymorphism have already been demonstrated-δALA-dehydratase, Vitamin D
receptor gene and Hemochromatosis gene. These alleles determine the susceptibility of the individuals to lead. We present
here a case of a lead acid battery worker, who presented without any signs and symptoms of lead poisoning except for a very
high level of blood lead (82.8μg/dl and 47.5μg/dl 9 months later) 相似文献
9.
Jafar T Agrawal S Mahdi AA Sharma RK Awasthi S Agarwal GG 《Indian journal of clinical biochemistry : IJCB》2011,26(3):296-302
The pathogenesis of idiopathic nephrotic syndrome is not completely understood. We postulate that cytokine gene polymorphisms
may influence susceptibility or clinical course in Idiopathic Nephrotic Syndrome. Polymorphisms of IL-4, IL-6, and TNF-α cytokines
were investigated in 150 children with Idiopathic Nephrotic Syndrome and 569 healthy controls by using polymerase chain reaction
and restriction fragment length polymorphism. On comparing patient with controls strong association were found for IL-6, TNF-α
and IL-4 at allelic level (IL-6-G174C (G vs. C): P = <0.001; OR = 6.33, TNF-α-G308A (G vs. A): P = <0.001; OR = 1.99, IL-4-C590T (C vs. T): P = 0.048; OR = 1.38). Further when SR group was compared with SS group significant association was found at genotypic level
in all the studied genetic polymorphisms. Studied cytokine gene polymorphisms may influence susceptibility to idiopathic nephrotic
syndrome and might affect steroid response in INS patients. 相似文献
10.
Ekaidem IS Bolarin DM Udoh AE Etuk SJ Udiong CE 《Indian journal of clinical biochemistry : IJCB》2011,26(2):187-192
Plasma fibronectin (FN) levels in obese/overweight and non-obese pregnant women were evaluated as a possible risk factor for
preeclampsia. A total of one hundred and sixty three pregnant women attending antenatal clinic at University of Calabar Teaching
Hospital participated in the study and sixty non-pregnant women served as control. About 77 (47.24%) of the pregnant women
were followed up for any subsequent development of preeclampsia during the pregnancy. Fibronectin levels in plasma were measured
by ELISA assay and serum total protein, urea and creatinine were determined spectrophotometrically. The mean plasma FN concentration
of non-obese pregnant women in first trimester was lower than those of the non-pregnant women by 24%, but however, increased
to the non-pregnant level in second and third trimesters. Obese/overweight pregnant women had significantly (P < 0.05) higher values than non-obese pregnant women in second and third trimesters. FN in obese/overweight pregnant women
correlated positively with mean arterial blood pressure (MAP: r = 0.414, P = 0.04). About 28.57% of the pregnant women with FN above cut off point of 330 μg/ml at 18–24 weeks of gestation developed
preeclampsia. This value increased to 40.0% when only the obese/overweight women were considered. On analysis of both fibronectin
>330 μg/ml and MAP > 90, the predictive value increased to 66.7%. We therefore conclude that elevated FN may be regarded as
a risk factor of preeclampsia especially among the obese women. 相似文献
11.
M. A. Qadar Pasha R. B. Ram M. D. Gupta 《Indian journal of clinical biochemistry : IJCB》2000,15(1):29-35
Determination of ammonia level in blood is important, especially in the diagnosis of hepatic disorders. An indigenously purified
enzyme was used in the standardisation of the assay. The assay is a two reagent system, requires five minutes for completion
and can be performed at temperature between 25–27°C. Performance of the assay was assessed by linearity, imprecision, functional
sensitivity and interference studies. Lyophilised reagent I and reagent II were found stable for at least one year. The plasma
level of ammonia for the controls was 13.7±7.3 μMol/L, whereas for subjects of hepatic disorders, it was 69.1±32.4 μMol/L
(P<0.001). The functional sensitivity was between 2–1000 μMol/L. Within-run coefficient of variation was between 1.1–2.0%
and between-run coefficient of variation was between 1.9–3.7%. The mean recovery after dilution was 99.6%. The present method
can estimate ammonia up to 1000 μMol/L without dilution of sample. Assay time of five minute may be shortened to one minute.
This method is suited for routine clinical use in treatment of hepatic disorders. 相似文献
12.
Zubieta-Calleja G Zubieta-Castillo G Zubieta-Calleja L Ardaya-Zubieta G Paulev PE 《Indian journal of clinical biochemistry : IJCB》2011,26(1):62-65
As the oxygen tension of inspired air falls with increasing altitude in normal subjects, hyperventilation ensues. This acute
respiratory alkalosis, induces increased renal excretion of bicarbonate, returning the pH back to normal, giving rise to compensated
respiratory alkalosis or chronic hypocapnia. It seems a contradiction that so many normal people at high altitude should permanently
live as chronic acid–base patients. Blood gas analyses of 1,865 subjects at 3,510 m, reported a P
aCO2 (arterial carbon dioxide tension ± SEM) = 29.4 ± 0.16 mmHg and pH = 7.40 ± 0.005. Base excess, calculated with the Van Slyke
sea level equation, is −5 mM (milliMolar or mmol/l) as an average, suggesting chronic hypocapnia. THID, a new term replacing
“Base Excess” is determined by titration to a pH of 7.40 at a P
aCO2 of 5.33 kPa (40 mmHg) at sea level, oxygen saturated and at 37°C blood temperature. Since our new modified Van Slyke equations
operate with normal values for P
aCO2 at the actual altitude, a calculation of THID will always result in normal values—that is, zero. 相似文献
13.
Soma Gupta M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2004,19(1):138-141
Lipid peroxidation product, malonaldehyde (MDA) and antioxidants were estimated in plasma and erythrocytes of 34 cases of
oral submucous fibrosis (OSMF) of different grades with equal number of healthy controls to evaluate the association of reactive
oxygen species (ROS) and OSMF. While plasma MDA was found to be significantly higher in patients (3.3±0.4 nmole/ml, P<0.001)
as compared to controls (2.4±0.5 nmole/ml), plasma beta carotene and vitamin E levels were found to be decreased significantly
in patients (81.7±14.3 μg/100 ml, P<0.001; 9.3±0.9 mg/L, P<0.01 respectively) with respect to healthy controls (110±20.8 μg/100
ml and 10.1±1.2 mg/L). The decrease in beta-carotene and vitamin E was found to be more significant in OSMF grade II and III
than in grade I. After 6 weeks of oral administration of beta-carotene and vitamin E, patients showed increase in plasma level
of these two antioxidants along with decrease in MDA level associated with clinical improvement. 相似文献
14.
Savita Singh Varun Malhotra K. P. Singh S. B. Sharma S. V. Madhu O. P. Tandon 《Indian journal of clinical biochemistry : IJCB》2001,16(2):216-220
Nineteen subjects of non-insulin dependent diabetes mellitus (NIDDM) between the age group of 30–60 yrs were studied to see
the effect of specific yoga asanas on fasting and postprandial blood glucose (FBG, PPG), serum malondialdehyde (MDA) and glycosylated
hemoglobin (HbA1) in addition to drug treatment and diet control. The duration of diabetes ranged from 1–10 years. Patients with renal, cardiac
and proliferative retinal diseases were excluded from the study. The same patients served as their own control. Subjects were
called in the morning to the cardio-respiratory laboratory and were given training by a yoga expert. Yoga asanas included
Suryanamskar, Tadasan, TriKonasan, Padmasan, Pranayam, Paschimottanasan, Ardhmatsyendrasan, Pavanmukthasan, Sarpasan and Shavasan.
The asanas were done every day for 40 days for 30–40 min. FBG, PPG, serum MDA and HbA1 were estimated before and after 40 days of yoga asanas regimen. Significant reduction was seen in FBG from 220 mg/dl to 162
mg/dl, PPG from 311 mg/dl to 255 mg/dl, MDA from 6 nmol/l to 3 nmol/l and HbA1, from 8.8% to 6.4%. Subjects felt better and were relieved of their stresses and had an improvement in their day to day performance.
The decrease was statistically significant (p<0.0001 for FBG and PPG, p<0.001 for MDA and for HbA1). 相似文献
15.
Mahajan RS Veerpathran A Dakshinamoorthy G Sharma RD Goswami K Reddy MV 《Indian journal of clinical biochemistry : IJCB》2010,25(4):362-366
WHO-Tropical Disease Research scheme highlighted the need for development of new anti-filarial drugs. Certain antibiotics
have recently been found effective against Wolbachia, co-existing symbiotically with filarial parasites. Inflammatory response
entails oxidative mechanism to educe direct anti-microbial effect. In the present study microfilariae were maintained in vitro
in medium supplemented with varying concentrations of tetracycline, doxycycline (20–100 μg/ml) or ciprofloxacin (50–250 μg/ml)
separately to find out any involvement of oxidative mechanism in the anti-filarial effect of these antibiotics. Loss of motility
of the microfilariae was measured after 48 h and correlated with the levels of MDA, nitric oxide and protein-carbonylation.
Significant loss of microfilarial motility was recorded with increasing concentration of tetracycline and doxycycline but
with ciprofloxacin the effect was not marked. Agents with high antifilarial activity revealed significant association with
oxidative parameters in a dose dependent manner. The result suggests that oxidative effect might be exploited to design novel
antifilarial drug candidate. 相似文献
16.
Graves’ disease (GD) is an organ-specific heterogenous autoimmune disorder associated with T-lymphocyte abnormality affecting
the thyroid, eyes and skin. GD is a multifactorial disease that develops as a result of complex interaction between genetic
susceptibility genes and environmental factors. It has been suggested that the Cytotoxic T lymphocytes associated molecule-4
(CTLA-4) is a genetic susceptibility candidate for GD. The present study was focused on A/G polymorphism at position 49 in
exon-1 of the CTLA-4 gene in 80 GD patients (GP) and 80 sex and age matched healthy individuals among South Indian (Madurai)
population. Serum concentrations of thyroid hormone (T4, T3 and TSH) were determined by using automated analyzer. The genomic DNA was isolated from the patient and control groups and
genotyping was performed using the polymerase chain reaction followed by restriction enzyme analysis using Bbv1. Significant difference (P < 0.001) was observed in the level of T3, T4 and TSH in GD patients and healthy individuals. The results revealed the CTLA-4 gene G/G genotype to be 32 (40%) in patients
and 26 (32.50%) in healthy individuals, A/G genotype to be 37 (46.25%) in patients and 25 (31.25%) in healthy individuals
and A/A genotype to be 11 (13.75%) in patients and 29 (36.25%) in healthy individuals. The calculated odds ratio (OR) in individuals
with mutant genotype (GG/AG) reveal 3.6 fold risk for GD (95% confidence interval = 1.6–7.8). The mutant “G” allele frequency
was observed to be 0.63 in GD patients and 0.48 in healthy individuals. Thus the present study demonstrates an association
between the CTLA-4 gene polymorphism and Graves’ disease. 相似文献
17.
Bincy Verghese Sonu Bhatnagar Ramchander Tanwar Jayashree Bhattacharjee 《Indian journal of clinical biochemistry : IJCB》2011,26(4):373-377
Psoriasis is chronic autoimmune hyperproliferative skin disease with a population prevalence of 1.5–3%. The cause of psoriasis
is still not fully understood. It has been hypothesized to be an immune-mediated disorder in which the excessive reproduction
of keratinocytes is due to cytokines such as interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha, secreted by infiltrating
CD4+ and CD8+ T cells and natural killer cells. The aim of our study was to determine the serum levels of TNF-α, IL-4, IL-6 & IL-10 in
psoriasis patients and compare it with healthy controls. 30 clinically diagnosed psoriasis patients and 30 age and sex matched
healthy controls were included in the study. The serum cytokine levels were measured by solid phase sandwich ELISA (DIACLONE
Research, France). TNF-α and IL-6 levels were significantly raised in patients and the results were statistically significant
(P < 0.001). IL-4 levels were higher in patients than in controls (1.91 ± 4.7 pg/ml in cases & 0.9 ± 0.3 pg/ml in controls)
but were not statistically significant. Interestingly, IL-10 levels were found to be higher in controls than in patients but
again, it was not statistically significant. Pro-inflammatory cytokines play a pivotal role in the pathogenesis of psoriasis
and it is the type 1(TH1) cytokine pattern, i.e., IL-6 & TNF-α, which predominate in the psoriatic T cell response. Further
studies on IL-10 levels in psoriasis are recommended to establish their exact role in the pathogenesis of the disease. 相似文献
18.
We recruited 59 individuals of known HIV serostatus after informed consent however, 44 were serodiscordant heterosexual partners
[serodiscordant seronegative (SSN group) and serodiscordant seropositive (SSP group)] while 15 were seronegative healthy individuals
(SNH). In the case–control study we choose to determine Malondialdehyde (MDA) concentration as a marker of lipid peroxidation
index (oxidative stress) spectrophotometrically and quantify HIV mRNA by Real Time-nucleic acid sequence based amplification
assay (RT-NASBA). Here our result show for the first time a high concentration of lipid peroxidation product (MDA, 116.6%)
with a significant (P < 0.05) increase in HIV serodiscordant seropositive subjects over their seronegative partners. However, Spearman rank correlation
statistics of SSP group showed a positive correlation value (P < 0.01, r = 0.89) between MDA and mRNA and a negative correlation between MDA and T-cell ratio (P < 0.01, r = 0.96).The study may strongly indicate a possible lipid peroxidation product threshold for predicting HIV infection and
progression in serodiscordant heterosexual partners. 相似文献
19.
The objective of this study was to evaluate acrosin activity in spermatozoa of infertile Nigerian men and to compare with
those of the fertile men. The acrosin activity was evaluated using the Acroscreen reagent kit. The acrosin activity of the
fertile men was 40.7±5.2 mIU/106 sperm (range 30.1–51.3) and those of the infertile men was 22.4±8.33 mIU/106 sperm (range 5.7–39.1). The difference in the mean was statistically significant (P<0.001). There was a subpopulation of
the infertile men who had their acrosin activity within normal range of the fertile men, 32.9±1.57 mIU/106 sperm. But the difference in the mean was statistically significant (P<0.001). Acrosin activity decreased with increased
morphological changes in the spermatozoa. It is concluded that acrosin activity in the infertile Nigerian men is significantly
lower than that in the fertile men. Acrosin activity may also be affected by morphological changes in the spermatozoa. 相似文献
20.
Emokpae MA Uwumarongie OH Osadolor HB 《Indian journal of clinical biochemistry : IJCB》2011,26(1):57-61
Proteinuria in subjects with sickle cell anaemia (SCA) is an indication of an ongoing renal insufficiency and it’s prevalence
varies between sexes. We evaluated sex differences in the activities of Lecithin: cholesterol acyltransferase (LCAT), Lipoprotein
lipase (LPL) and the levels of lipoproteins in SCA patients with proteinuria. Fifty SCA patients (30 males aged: 26.4 ± 7.3 years
and 20 females, aged 25.4 ± 2.6 years) and 50 age and sex matched control SCA patients were recruited for the study. Random
urine specimens were collected and tested for the presence of albumin by urine dipstick technique. A 24 h urinary protein
was quantitated using sulphosalicylic acid technique. Fasting serum total cholesterol, triglyceride, urea and creatinine were
determined using enzymes catalyzed colorimetric methods. HDL cholesterol was determined in the supernatant after precipitation
with manganese chloride–phosphotungstic acid solution. LCAT was measured using the Anasolv LCAT assay with proteoliposome
as substrate. LPL was determined by incubating the serum in glyceryl trioleate substrate, the glycerol liberated was measured
in an aliquot of the incubating mixture. In male SCA controls there was 18.2 and 6.9% increase in the activities of LPL and
LCAT respectively when compared with females but in SCA patients with proteinuria there was 8.4 and 5.2% decreases in the
male SCA patients compared with females. The concentration of 24 h urine protein in the SCA male subjects with proteinuria
was significantly higher (0.25 g/day; P < 0.001) compared with the SCA female patients with proteinuria (0.09 g/day). There are sex differences in the activities
of LCAT and LPL in SCA patients with proteinuria. Metabolism of these lipolytic enzymes may be modulated differently in SCA
patients with proteinuria. 相似文献