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Hai-ping LU Martin EDWARDS Qi-zhao WANG Hai-jun ZHAO Hao-wei FU Jian-zhong HUANG Angharad GATEHOUSE Qing-yao SHU 《Journal of Zhejiang University. Science. B》2015,16(2):113-122
目的:分析CYP81A6基因在苯达松及甲磺隆处理下的诱导表达模式,解释该基因与两种除草剂代谢相关的可能原因。创新点:从两种除草剂降解途径中产生的小分子物质的结构相似性出发,通过基因诱导表达的特点分析,解释CYP81A6和两种除草剂降解相关的原因。方法:通过实时定量聚合酶链反应(PCR)来分析基因表达的特点;利用CYP81A6启动子与GUS报告基因构建的载体来分析组织特异性表达;通过亚细胞定位来确定CYP81A6发挥功能的场所。结论:CYP81A6基因受苯达松及甲磺隆诱导,在不同的时间点开始上调,说明了甲磺隆的降解中间产物可以诱导这个基因的表达;CYP81A6是组成型表达,在根、茎、叶中均有表达;亚细胞定位结果证明CYP81A6是一个内质网上的蛋白。 相似文献
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枇杷核中苦杏仁苷的分离纯化 总被引：1，自引：0，他引：1
以莆田"解放钟"枇杷核为原料,采用水提工艺,筛选出一种吸附性能较好的树脂,并对其进行了苦杏仁苷的分离纯化研究。结果表明采用大孔吸附树脂HZ801进行苦杏仁苷的富集纯化较佳,并确定优化枇杷核中苦杏仁苷大孔吸附树脂HZ801富集纯化工艺。 相似文献
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Qi-chao ZHAO Ming-hong LIU Xian-wen ZHANG Chao-yang LIN Qing ZHANG Zhi-cheng SHEN 《Journal of Zhejiang University. Science. B》2015,16(10):824-831
Insect resistance and glyphosate tolerance have been two of the most important traits in the genetic improvement of various crops. In this study, two Bacillus thuringiensis (Bt) insecticidal genes, Cry1Ac and Cry1Ig, and a modified glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene (G10) were combined into a single transferred DNA (T-DNA) fragment and introduced into rice by Agrobacterium-mediated transformation. A transgenic line with single-copy T-DNA insertion named GAI-14 was found to be highly resistant to striped stem borer and rice leaf roller, and tolerant to glyphosate. Analysis of T-DNA border sequence suggested that the transgenes were inserted at the chromosome 3 and appeared to have not interrupted any known or putative genes. A field trial observed no significant difference in the basic agronomic traits between GAI-14 and the recipient rice. 相似文献
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Miao-miao Liu Xiao-jing Zhang Yan Gao Zhi-cheng Shen Chao-yang Lin 《Journal of Zhejiang University. Science. B》2018,19(8):610-619
A transgenic maize event ZD12-6 expressing a Bacillus thuringiensis (Bt) fusion protein Cry1Ab/Cry2Aj and a modified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) protein G10 was characterized and evaluated. Southern blot analysis indicated that ZD12-6 is a single copy integration event. The insert site was determined to be at chromosome 1 by border sequence analysis. Expression analyses of Bt fusion protein Cry1Ab/Cry2Aj and the EPSPS protein G10 suggested that they are both expressed stably in different generations. Insect bioassays demonstrated that the transgenic plants are highly resistant to Asian corn borer (Ostrinia furnacalis), cotton boll worm (Helicoverpa armigera), and armyworm (Mythimna separata). This study suggested that ZD12-6 has the potential to be developed into a commercial transgenic line. 相似文献
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Zhang-yue Song Jing-luan Tian Wei-zhe Fu Lin Li Ling-hong Lu Lian Zhou Zhi-hui Shan Gui-xiang Tang Hui-xia Shou 《Journal of Zhejiang University. Science. B》2013,14(4):289-298
The Agrobacterium-mediated transformation system is the most commonly used method in soybean transformation. Screening of soybean genotypes favorable for Agrobacterium-infection and tissue regeneration is the most important step to establish an efficient genetic transformation system. In this study, twenty soybean genotypes that originated from different soybean production regions in China were screened for transient infection, regeneration capacity, and stable transgenic efficiency. Three genotypes, Yuechun 04-5, Yuechun 03-3, and Tianlong 1, showed comparable stable transgenic efficiencies with that of the previously reported American genotypes Williams 82 and Jack in our experimental system. For the Tianlong 1, the average stable transformation efficiency is 4.59%, higher than that of control genotypes (Jack and Williams 82), which is enough for further genomic research and genetic engineering. While polymerase chain reaction (PCR), LibertyLink strips, and β-glucuronidase (GUS) staining assays were used to detect the insertion and expression of the transgene, leaves painted with 135 mg/L Basta could efficiently identify the transformants. 相似文献
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A machine vision system was developed to inspect the quality of rice seeds. Five varieties of Jinyou402, Shanyou10, Zhongyou207, Jiayou and IIyou were evaluated. The images of both sides of rice seed with black background and white background were acquired with the image processing system for identifying external features of rice seeds. Five image sets consisting of 600 original images each were obtained. Then a digital image processing algorithm based on Hough transform was developed to inspect the rice seeds with incompletely closed glumes. The algorithm was implemented with all image sets using a Matlab 6.5 procedure. The results showed that the algorithm achieved an average accuracy of 96% for normal seeds, 92% for seeds with fine fissure and 87% for seeds with incompletely closed glumes. The algorithm was proved to be applicable to different seed varieties and insensitive to the color of the background. 相似文献
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GMP对制药厂房的空气质量指标有着严格的要求,而净化空调系统是保证洁净厂房内空气质量的最主要的设备。为了提供净化空调系统的管理,本文整合了DDC控制技术与变频调节技术,提出了在净化空调中采用DDC和变频器进行控制的方案,该方案既可净化空气的各项指标达到GMP的要求,保证净化厂房内的洁净度,又可保证空调系统有效、节能运转。 相似文献
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Machine vision inspection of rice seed based on Hough transform 总被引：1，自引：0，他引：1
INTRODUCTION It is a common phenomenon that glumes ofrice seeds are incompletely closed, especially forhybrid rice. The average proportion of hybrid riceseed with incompletely closed glumes is about 26%in nature. The threshing and drying in post-harvesthandling will further increase the proportion. Thegermen segmentation is inhibited by ultravioletillumination from sunshine while the germen epi-dermis cell stops growing because of shortage ofwater due to the limited moisture. These … 相似文献
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本模拟研究的目标组与参照组的项目作答反应数据是通过Rasch模型产生的,模拟研究探讨了LRDIF检测方法在不同DIF比例和纯化方式下的检测效果。研究结果表明:LRDIF方法的检测结果在DIF比例≤40%时是可信的;当采用LRDIF方法进行DIF检测时,有必要对匹配变量进行纯化,最好是能够进行迭代纯化。 相似文献
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邓宁 《商丘师范学院学报》1996,(Z4)
质粒ＤＮＡ的提纯是现代分子遗传学和基因工程的重要技术，本实验对质粒ＤＮＡ的提取和纯化方法进行了比较研究．并通过改进，建立了简便、快速提取和纯化质粒ＤＮＡ的方法．利用此法所提质粒ＤＮＡ收率大，纯度高，能适于细菌转化、细胞转染及酶切分析等进一步实验研究． 相似文献
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孙艳梅 《山西广播电视大学学报》2004,(4):33-34
教师要净化自己的教学语言 ,提高普通话口语表达能力 ,掌握专业术语 ,力求使教学语言准确、简洁、优美 ,并不断提高自己的语言修养和语言素质 ,为学生营造一个良好的语言环境 相似文献
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The affinity heptapeptide(HWWWPAS)for insulin,selected from phage display library,was coupled to EAH Sepharose 4B gel and packed to a 1-mL column.The column was used for the affinity purification of insulin from protein mixture and commercial insulin preparation.It was observed that the minor impurity in the commercial insulin was removed by the affinity chromatography.Nearly 40 mg of insulin could be purified with the 1-mL affinity column.The results revealed the high specificity and capacity of the affinity column for insulin purification.Moreover,based on the analysis of the amino acids in the peptide sequence,shorter peptides were designed and synthesized for insulin chromatography.As a result,HWWPS was found to be a good alternative to HWWWPAS,while the other two peptides with three or four amino acids showed weak affinity for insulin.The results indicated that the peptide sequence of HWWWPAS was quite conservative for specific binding of insulin. 相似文献
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董倩 《南昌教育学院学报》2012,(7):32+44
乔伊斯的名著《死者》中有多处关于雪景的描述,“雪”不仅给人展现了一幅震撼人心的情境,同时也净化了人们的灵魂.本文将用亚里士多德“净化说”来分析“雪”及所谓“死者”的象征意义,并解析作者对于生命意识的虚无和现实性的对立与统一的观照 相似文献
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Ross Shegog Melanie M. Lazarus Nancy G. Murray Pamela M. Diamond Nathalie Sessions Eva Zsigmond 《Research in Science Education》2012,42(5):875-890
The transgenic mouse model is useful for studying the causes and potential cures for human genetic diseases. Exposing high school biology students to laboratory experience in developing transgenic animal models is logistically prohibitive. Computer-based simulation, however, offers this potential in addition to advantages of fidelity and reach. This study describes and evaluates a computer-based simulation to train advanced placement high school science students in laboratory protocols, a transgenic mouse model was produced. A simulation module on preparing a gene construct in the molecular biology lab was evaluated using a randomized clinical control design with advanced placement high school biology students in Mercedes, Texas (n?=?44). Pre-post tests assessed procedural and declarative knowledge, time on task, attitudes toward computers for learning and towards science careers. Students who used the simulation increased their procedural and declarative knowledge regarding molecular biology compared to those in the control condition (both p?<?0.005). Significant increases continued to occur with additional use of the simulation (p?<?0.001). Students in the treatment group became more positive toward using computers for learning (p?<?0.001). The simulation did not significantly affect attitudes toward science in general. Computer simulation of complex transgenic protocols have potential to provide a ??virtual?? laboratory experience as an adjunct to conventional educational approaches. 相似文献
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An increasing number ofmonopartite begomoviruses are being identified that a satellite molecule (DNAβ) is required to induce typical symptoms in host plants. DNAβ encodes a single gene (termed βCl) encoded in the complementary-sense. We have produced transgenic Nieotiana benthamiana and N. tabaeum plants expressing the βC1 gene of a DNAβ associated with Tomato yellow leaf curl China virus (TYLCCNV), under the control of the Cauliflower mosaic virus 35S promoter. Transgenic plants expressing 13C1 showed severe developmental abnormalities in both species. Microscopic analysis of sections of both transgenic and non-transgenic N. tabaeum leaves showed abnormal outgrowths of transgenic N. tabaeum to be due to disorganizedcell division (hyperplasia) of spongy and palisade parenchyma. Immuno-gold labeling of sections with a polyclonal antibody against the βC1 protein showed that the 13C 1 protein accumulated in the nuclei of cells. The possible biological function of the βC1 1protein was discussed. 相似文献
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Transgenic Brassica compestris L.spp.chinensis plants expressing a choline oxidase(codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation.In the transgenic plants,codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay(ELISA) examination,immunogold localization,and 1 H-nuclear magnetic resonance( 1 H-NMR) . Stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions.The plants of transgenic line 1(L1) showed significantly higher net photosynthetic rate(Pn) and Pn recovery rate under high(45°C,4 h) and low temperature(1°C,48 h) treatments,and higher photosynthetic rate under high salinity conditions(100,200,and 300 mmol/L NaCl,respectively) than the wild-type plants.The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine,which is not found in the wild-type plants.Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L.spp.chinensis could be a potential strategy for improving the plant tolerance to multiple stresses. 相似文献