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1.
Qing-song Xi Li-xia Zhu Juan Hu Li Wu Han-wang Zhang 《Journal of Zhejiang University. Science. B》2012,13(9):717-722
Objective
To reevaluate whether relatively few oocytes obtained in one cycle are an indication for intracytoplasmic sperm injection (ICSI).Methods
A total of 406 cycles with three or fewer retrieved oocytes performed in 396 non-male infertile couples were retrospectively reviewed. Cycles were classified into three groups by different fertilization techniques: the in vitro fertilization (IVF) group, insemination with conventional IVF; the ICSI group, insemination with ICSI though semen parameters were normal; and the rescue ICSI group, re-insemination with ICSI after conventional IVF failure.Results
The ICSI group resulted in higher normal fertilization compared with the conventional IVF group. Correspondingly, the cycle cancellation rate was decreased in the ICSI group, though it was not statistically significant. The clinical pregnancy rate and implantation rate were lower in the ICSI group compared with the conventional IVF group. Rescue ICSI was a method to avert total fertilization failure in conventional IVF, increasing fertilization and ensuring embryo availability for transfer, but the normal fertilization was the lowest due to delayed insemination and the chance of pregnancy was very little.Conclusions
Obtaining only few oocytes in one cycle is not considered as an indication for ICSI when the sperm sample is apparently normal. Rescue ICSI is either not recommended if conventional insemination fails. Such patients should not be subjected to the unnecessary costs and potential risks of ICSI. 相似文献2.
Objective: Embryonic chromosomal abnormality is one of the main reasons for in vitro fertilization (IVF) failure. This study aimed at evaluating the value of Fluorescence in-situ Hybridization (FISH)-based Preimplantation Genetic Diagnosis (PGD) in screening for embryonic chromosomal abnormality to increase the successful rate of IVF. Method: Ten couples, four with high risk of chromosomal abnormality and six infertile couples, underwent FISH-based PGD during IVF procedure. At day 3, one or two blastomeres were aspirated from each embryo. Biopsied blastomeres were examined using FISH analysis to screen out embryos with chromosomal abnormalities. At day 4, embryos without detectable chromosomal abnormality were transferred to the mother bodies as in regular IVF. Results: Among 54 embryos screened using FISH-based PGD, 30 embryos were detected to have chromosomal abnormalities. The 24 healthy embryos were implanted, resulting in four clinical pregnancies, two of which led to successful normal birth of two 相似文献
3.
Objective: Embryonic chromosomal abnormality is one of the main reasons for in vitro fertilization (IVF)failure. This study aimed at evaluating the value of Fluorescence in-situ Hybridization (FISH)-based Preimplantation Genetic Diagnosis (PGD) in screening for embryonic chromosomal abnormality to increase the successful rate of IVF. Method:Ten couples, four with high risk of chromosomal abnormality and six infertile couples, underwent FISH-based PGD during IVF procedure. At day 3, one or two blastomeres were aspirated from each embryo. Biopsied blastomeres were examined using FISH analysis to screen out embryos with chromosomal abnormalities. At day 4, embryos without detectable chromosomal abnormality were transferred to the mother bodies as in regular IVF. Results: Among 54 embryos screened using FISH-based PGD, 30 embryos were detected to have chromosomal abnormalities. The 24 healthy embryos were implanted,resulting in four clinical pregnancies, two of which led to successful normal birth of two healthy babies; one to ongoing pregnancy during the writing of this article; and one to ectopic pregnancy. Conclusion: FISH-based PGD is an effective method for detecting embryonic chromosomal abnormality, which is one of the common causes of spontaneous miscarriages and chromosomally unbalanced offsprings. 相似文献
4.
Lu YH Gao HJ Li BJ Zheng YM Ye YH Qian YL Xu CM Huang HF Jin F 《Journal of Zhejiang University. Science. B》2012,13(1):1-10
To evaluate the effects of sperm with different parameters and sources on the outcomes of intracytoplasmic sperm injection
(ICSI), 1972 ICSI cycles were analyzed retrospectively. Groups 1 to 5 were composed of cycles using ejaculated sperm and were
grouped according to sperm quantity, quality, and morphology into normal (288 cycles), or mild (329 cycles), moderate (522
cycles), severe (332 cycles), and extremely severe (171 cycles) oligozoospermia and/or asthenozoospermia and/or teratozoospermia
(OAT) groups. Group 6 was composed of 250 cycles using testicular or epididymal sperm, and Group 7 consisted of 80 cycles
using frozen-thawed sperm. We found that fertilization rates were gradually reduced from Groups 1 to 6, and reached statistical
difference in Groups 5 and 6 (P<0.05). The high-quality embryo rate was higher in Group 1 than in Groups 2, 3, 5, 6, and 7 (P<0.05). No statistical differences were observed in the rates of embryo cleavage, clinical pregnancy, miscarriage, live-birth,
premature birth, low birth weight, weeks of premature birth, average birth weight, or sex ratio for all seven groups (P>0.05). A total of nine cases of malformation were observed, with a malformation rate of 1.25% (9/719). In conclusion, different
sperm sources and parameters can affect ICSI outcomes before embryo implantation. A full assessment of offspring malformation
will require further study using a larger sample size. 相似文献
5.
Zhang Y Xu CM Zhu YM Dong MY Qian YL Jin F Huang HF 《Journal of Zhejiang University. Science. B》2007,8(7):515-521
Objective： To evaluate the effect ofpreimplantation genetic diagnosis （PGD） conducted for women who had Down syndrome pregnancy previously. Methods： Trisomy 21 was diagnosed by using fluorescence in site hybridization （FISH） before embryo transfer in two women who had Down syndrome pregnancies. Each received one or two PGD cycles respectively. Results： Case 1： one PGD cycle was conducted, two oocytes were fertilized and biopsied. One embryo is of trisomy 21 and the other of monosomy 21. No embryo was transferred. Case 2： two PGD cycles were conducted, in total, sixteen oocytes were fertilized and biopsied. Four embryos were tested to be normal, six of trisomy 21, and one of monosomy 21. Five had no signal. Four normal embryos were transferred but no pregnancy resulted. Conclusion： For couples who had pregnancies with Down syndrome previously, PGD can be considered, and has been shown to be an effective strategy. 相似文献
6.
Objective: We dynamically measured serum inhibin B and estradiol in the early stage of hormonal stimulation to predict the ovarian response in in vitro fertilization (IVF) treatment. Methods: A total of 57 patients (<40 years of age) who un-derwent the first cycle of long protocol IVF or introcytoplasmic sperm injection (ICSI) treatment were included. Serum inhibin B, estradioi, follicle stimulating hormone (FSH) and luteinizing hormone (LH) levels were measured four times: (i) on Day 3 of the menstrual cycle (basal); (2) on the day before the first administration of gonadotrophin (Gn) (Day 0); (3) on Day 1 of Gn therapy; and (4) on Day 5 of Gn therapy. Comparisons of these measurements with ovarian responses and pregnancy outcomes were made and analyzed statistically. Results: (1) On Day 1 and Day 5 of recombinant FSH (rFSH) stimulation, ovarian response, i.e., numbers of follicles, oocytes, fertilized oocytes, and embryos, had a positive correlation (rS=0.46～0.61, P=0.000) with raised inhibin B and estradiol concentrations, but a negative correlation (rS=-0.67～-0.38, P=0.000 or P<0.01) with total rFSH dose and total days ofrFSH stimulation. (2) No significant variation (P>0.05) between the pregnant and non-pregnant groups on the basis of mean age or on all hormone concentrations at four times of the IVF cycle was observed. However, all the seven patients aged >35 years did not reach pregnancy. Conclusions: (1) Serum inhibin B and estradiol concentrations obtained shortly after Gn therapy may offer an accurate and early prediction of ovarian response; (2) Low levels of serum inhibin B and estradiol obtained shortly after Gn stimulation indicate the need for a longer period of Gn treatment and a higher daily dosage; (3) No obvious pregnancy difference among patients of age <35 years was found; however, IVF pregnancy outcome is significantly lower in women of age >35 years. 相似文献
7.
Objective: To investigate the factors that might influence the success of an embryo freezing and thawing program. Method:
The relationship between the pregnancy rate in 73 cycles of embryo, freezing and thawing program and the following factors
was analyzed: maternal age,E
2 level at the time of HCG trigger, embryo storage time, number of thawed embryos transferred, presence of sponsoring embryos
and intact embryos. And the survival rate of thawed embryos with different morphology, cell stage and storage time was evaluated.
Result: Transfer with three or more than three thawed embryos resulted in pregnancy rates of 38.5% and 35.7%, respectively,
compared with 5.3% for transfer of fewer than three embryos. The presence of sponsoring embryos and intact embryos significantly
increases pregnancy rate in embryo freezing and thawing program. No other factor examined had any effect on pregnancy outcome.
The survival rate of good morphology embryos was higher than poor ones, but was not influenced by cell stage and storage time.
Conclusion: Embryo morphology before freezing, number of thawed embryos transferred and the presence of intact embryos are
important to the outcome of embryo freezing and thawing program. 相似文献
8.
辅助生殖技术学龄前单双胎子代的认知发展存在差异么?(英文)简 总被引：1，自引：0，他引：1
Lan-feng XING Yu-li QIAN Lu-ting CHEN Fan-hong ZHANG Xin-fen XU Fan QU Yi-min ZHU 《Journal of Zhejiang University. Science. B》2014,15(1):51-57
Objective： To explore whether there exist differences in cognitive development between singletons and twins born after in vitro fertilization （IVF） or intracytoplasmic sperm injection （ICSI）. Methods： A total of 566 children were recruited for the study, including 388 children （singletons, n=175; twins, n=213） born after IVF and 178 children （singletons, n=87; twins, n=91 ） born after ICSI. The cognitive development was assessed using the Chinese-Wechsler Intelligence Scale for Children （C-WISC）. Results： For all pre-term offspring, all the intelligence quotient （IQ） items between singletons and twins showed no significant differences no matter if they were born after IVF or ICSI. There was a significant difference in the cognitive development of IVF-conceived full-term singletons and twins. The twins born after IVF obtained significantly lower scores than the singletons in verbal IQ （containing information, picture ＆ vocabulary, arithmetic, picture completion, comprehension, and language）, performance IQ （containing maze, visual analysis, object assembly, and performance）, and full scale IQ （P〈0.05）. The cognitive development of full-term sin- gletons and twins born after ICSI did not show any significant differences. There was no significant difference between the parents of the singletons and twins in their characteristics where data were collected, including the age of the mothers, the current employment status, the educational backgrounds, and areas of residence. There were also no consistent differences in the duration of pregnancy, sex composition of the children, age, and height between sin- gletons and twins at the time of our study although there existed significant differences between the two groups in the sex composition of the full-term children born after ICSI （P〈0.05）. Conclusions： Compared to the full-term singletons born after IVF, the full-term twins have lower cognitive development. The cognitive development of full-term singletons and twins born after ICSI did not show any significant differences. For all pre-term offspring, singletons and twins born after IVF or ICSI, the results of the cognitive development showed no significant differences. 相似文献
9.
目的:比较使用半量GnRHa与全量GnRHa,进口促性腺激素(Gn)与国产Gn在体外受精-胚胎移植(IVF-ET)控制性超排卵中的疗效和费用。方法:将142例不孕症患者分为4个组进行控制性促排卵:甲组采用半量GnRHa+国产HMG+HCG;乙组采用半量GnRHa+进口HP+HCG;丙组采用全量GnRHa+国产HMG+HCG,丁组采用全量GnRHa+进口HP+HCG。结果:四组平均获卵数、优质胚胎数、妊娠率及流产率、副作用卵巢过度刺激综合征(OHSS)及宫外孕的发生率无显著性差异;全量GnRHa组使用Gn的总量及天数多于半量GnRHa组;甲组(半量GnRHa+国产HMG)的医疗费用低于其它三组P<0.01)。结论:使用半量GnRHa+国产Gn可以较低的医疗费用获得与使用全量GnRHa及进口Gn相同的控制性超排卵效果。 相似文献
10.
11.
同源四倍体水稻双受精过程的细胞学观察 总被引：4，自引：0，他引：4
同源四倍体水稻的双受精比较特殊,花粉粒在其柱头上萌发比较慢,花粉管在花柱内的伸长比较迟缓,双受精延迟。退化胚囊的频率比较高（36．0％）,极核和反足细胞的形态异常,存在着一定频率的胚自发现象,由此认为,同源四倍体水稻的有性生殖能力已以明显变弱。 相似文献
12.
探讨了全氟辛烷磺酸盐(PFOS)对斑马鱼胚胎的发育毒性与氧化应激的影响。将斑马鱼胚胎暴露于系列浓度(0.1mg/L,0.5mg/L,1mg/L,5mg/L,10mg/L)的PFOS中,分别在胚胎受精后数小时(24hpf,48hpf,72hpf,96hpf,120hpf)内统计急性发育毒性指标,包括存活率、畸形率、心率以及孵化率。同时在暴露结束的120hpf,检测相关的抗氧化酶活性变化,主要包括超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(Gpx)以及脂质过氧化物(MDA)。结果显示,PFOS能够导致胚胎/幼鱼存活率降低,畸形率增加,心率加快以及孵化延迟;同时,胚胎SOD活性以及MDA水平增加,CAT与Gpx活性降低,表明PFOS能够诱发斑马鱼胚胎急性发育中毒,影响其抗氧化系统,导致胚胎产生氧化应激。 相似文献
13.
Kai Xu Ya-ling Han Quan-min Jing Shou-li Wang Ying-yan Ma Xiao-zeng Wang Geng Wang Zu-lu Wang 《Journal of Zhejiang University. Science. B》2012,13(8):624-630
Objective: There is no simple or feasible post-procedural intravascular ultrasound (IVUS) score to predict major adverse cardiac events (MACE) in patients undergoing drug-eluting stents (DES) implantation. The aim of this study is to validate a new IVUS score for predicting MACE. Methods: A total of 295 patients (with 322 lesions) were enrolled. IVUS score was calculated in each lesion based on five IVUS morphological characteristics: inflow/outflow disease, malapposition, underexpansion, tissue protrusion, and edge dissection (iMUTE score). We assigned two points to an underexpansion and one point for each presence of other factors. Patients were divided into low score (iMUTE score<2, n=137) and high score (iMUTE score≥2, n=158) groups. Results: At one year follow-up, a trend was seen in favor of the low iMUTE score group in MACE (3.65% vs. 10.10%; P=0.052), and there was more target vessel revascularization (TVR) in the high iMUTE score group compared with low score group (6.96% vs. 1.46%; P=0.044). Low iMUTE score was an independent predictor of freedom from TVR at one year (adjusted hazard ratio (HR) 0.5, 95% confidence interval (CI) 0.1–0.8; P=0.02). Conclusions: Post-procedural IVUS iMUTE scoring was simple and feasible in clinical practice, and can provide independent prognostic value for TVR in patients undergoing DES implantation. 相似文献
14.
INTRODUCTION Cataract is frequently associated with vitreo-retinal pathology such as vitreous hemorrhage,retinal detachment,proliferative vitreoretinal disease,espe-cially in patients with diabetes mellitus,ocular trauma and elderly population(Vatavuk and Pentz,2004;Vatavuk et al.,2005).The cataract can be removed together with vitrectomy or later on in a separate surgical procedure(Tyagi et al.,1998;Lam et al.,1998).The primary indication for the primary re-moval of cataract and vitrect… 相似文献
15.
李璟琦 《陕西教育学院学报》2007,23(3):64-66
以小偃22幼胚为试验材料,通过田间取样室内培养的方法研究了不同取材时期对小偃22幼胚体细胞胚性无性系诱导的影响。结果表明:小偃22未成熟种子占颖壳长度比例在60%~80%之间时,幼胚处于"半透明向淡黄色过渡"状态,愈伤组织诱导率达到95.6%以上,胚性愈伤组织诱导率达到39.4%~46.1%,是最佳培养时期。 相似文献
16.
Using generalizability (G-) theory and rater interviews as research methods, this study examined the impact of the current scoring system of the CET-4 (College English Test Band 4, a high-stakes national standardized EFL assessment in China) writing on its score variability and reliability. One hundred and twenty CET-4 essays written by 60 non-English major undergraduate students at one Chinese university were scored holistically by 35 experienced CET-4 raters using the authentic CET-4 scoring rubric. Ten purposively selected raters were further interviewed for their views on how the current scoring system could impact its score variability and reliability. The G-theory results indicated that the current single-task and single-rater holistic scoring system would not be able to yield acceptable generalizability and dependability coefficients. The rater interview results supported the quantitative findings. Important implications for the CET-4 writing assessment policy in China are discussed. 相似文献
17.
采用体视镜,相差显微镜和摄像机等器材观察萼花臂尾轮虫非多精卵的发育时间、形态和结构的变化。研究结果表明,轮虫的生长周期受温度影响较大,非受精卵的胚胎发育可分为两大阶段十一小阶段,发育时间为7～11小时。胚后发育分为两大阶段八小阶段,发育时间为27—42小时。轮虫非受精卵剥离母体后可以自行发育,母体对其发育有一定影响。 相似文献
18.
目的分析全身炎症反应综合征(systemic inflammatory response syndrome,SIRS)评分、序贯器官功能(sequential organ failure assessment,SOFA)评分及快速序贯器官功能(quick sequential organ failure assessment,qSOFA)评分在脓毒症早期筛查中的意义。方法:选取2016年5月30日-2018年5月30日收治于我院重症医学科患者232例感染或疑似感染患者为研究对象,对其病例资料进行回顾性分析。进行SIRS评分、SOFA评分及qSOFA评分,分析三种评分方法对脓毒症患者阳性检出率及28天病死率。结果:232例感染或疑似感染患者中,SIRS组检出141例,阳性检出率60.8%,28天病死率16.3%,SOFA组检出80例,阳性检出率34.5%,28天病死率22.5%,qSOFA组检出42例,阳性检出率18.1%,28天病死率38%。结论:SOFA评分和qSOFA评分能对感染性患者预后能做出较为准确的评估,qSOFA评分最优。 相似文献
19.
Xi-shao Xie Zhou-tao Xie Shi-long Xiang Xing-qun Yan Xiao-hui Zhang Zhang-fei Shou Jiang-hua Chen 《Journal of Zhejiang University. Science. B》2016,17(5):375-381
To describe the long-term clinical outcomes of patients with autosomal dominant polycystic kidney disease (ADPKD) who are on peritoneal dialysis (PD) therapy. We performed a retrospective matched-cohort analysis comparing the clinical outcomes of 30 ADPKD patients with those of 30 non-diabetic patients who had bilateral small kidneys between July 1 2007 and July 31 2014. The patient groups were matched by age, gender, and time of PD initiation. There were no significant differences in the demographic or biochemical parameters, comorbid conditions, residual glomerular filtration rate, or Charlson comorbidity score at the beginning of PD. The median renal volume was 1315 ml for the ADPKD group and 213 ml for the control group. Patients with ADPKD had similar 3-year patient survival (90.6% versus 86.3%, P=0.807) and technique survival (89.2% versus 74.3%, P=0.506) compared with non-ADPKD patients. Also, there was no significant difference in the peritonitis-free survival between the ADPKD and control groups (P=0.22), and rates of peritonitis were similar (0.19 versus 0.21 episodes per patient-year, P=0.26). No differences were observed in the incidence of PD-related complications, such as hernia and dialysate leak. ADPKD is not a contraindication for PD, and a subgroup of ADPKD patients with relatively small kidney volume can be treated using PD. 相似文献
20.
Lu Xiu-e Huang He-feng Li Mei-gen Zhu Yi-min Qiang Yu-li Dong Min-yue 《浙江大学学报(A卷英文版)》2005,6(9):897-902
Objectives: To measure serum and follicular resistin, steroids hormone levels in women with PCOS (polycystic ovary syndrome)
(BMI (body mass index)<25 kg/m2), to assess possible correlations of resistin to hormonal and metabolic parameters and to analyze the clinical outcomes of
in vitro fertilization-embryo transfer (IVF-ET) in women with PCOS and tubal infertility. Study design: We analyzed the clinical
outcomes of IVF-ET in women with PCOS (BMI<25 kg/m2) and tubal infertility during the years 2002 to 2004 and compared the serum and follicular fluid resistin levels, estradiol
(E2), progesterone (P), testosterone (T) levels in 20 PCOS and 20 healthy, age-matched women without PCOS during IVF-stimulated
cycles. The correlations between the resistin levels and the outcomes of IVF-ET were evaluated. Results: No significant differences
in resistin levels of either serum or follicular fluid between PCOS and control group were found. However, resistin levels
in serum were higher than that in follicular fluid in both groups. Multiple regression analysis showed that resistin levels
in serum did not correlate with BMI, estradiol, LH (luteinizing hormone) and insulin level in fasting blood. No significant
correlations were found between follicular fluid reisistin levels and fertilization rate, implantation rate, clinical pregnancy
rate or early miscarriage rate in both PCOS and control groups. Conclusion: Our results show that resistin does not have correlation
with the hormonal and metabolic parameters as well as the outcomes of IVF. These data suggest that resistin is unlikely to
be a local determinant factor in steroidogenesis and growth and maturation of oocytes during IVF-ET in lean women with PCOS. 相似文献