共查询到20条相似文献，搜索用时 171 毫秒
1.
柚类果皮中柚皮苷含量的测定分析 总被引：1，自引：0，他引：1
运用HPLC方法对7个柚品种果皮中柚皮苷含量进行测定,并结合统计分析的方法对各品种进行比较,结果表明:各品种果皮柚皮苷含量由高到低依次为:晚白柚、安江香柚、梁平柚、锅魁柚、蓬溪柚、长寿沙田柚、垫江白柚。晚白柚与安江香柚、梁平与锅魁柚差异显著,其余品种间差异极显著。 相似文献
2.
由大戟科大戟属多年生灌木一品红 (Euphorbiapulcherrimawilld)幼叶诱导形成的愈伤组织 ,在MS +NAA 0 .5mg .L-1(以下单位相同 )的培养基上培养 10— 15d后的愈伤组织 ,在光照下植于MS +ZT1.0 +NAA0 .1培养基上培养 ,芽分化率为 6 6 4 %。愈伤组织先在MS +2 ,4 -D1.0的培养基上预培养 2 0d ,再转入MS +ZT1.0 +NAA0 .1的培养基上培养 ,芽分化率明显提高 ,分化率为 89 3% ,但是在仅有 2 ,4 -D的培养基上培养未见芽形成 相似文献
3.
研究不同直径大小柚果中总黄酮、总酚、柚皮苷、橙皮苷和野漆树苷含量的变化及黑曲霉对柚果黄酮类成分的影响.无菌柚果接入黑曲霉，恒温恒湿培养下于不同时间点进行取样.采用紫外分光光度法和高效液相色谱法分别测定不同直径大小柚果及黑曲霉发酵后柚果中总黄酮、柚皮苷、橙皮苷和野漆树苷的含量.不同直径大小各柚果总黄酮、总酚和柚皮苷的含量随柚果直径的增大而下降.黑曲霉反接后，发酵组总黄酮、柚皮苷和野漆树苷含量呈下降趋势，橙皮苷含量先增后减，在发酵第36 h达到最高.柚果直径、黑曲霉发酵均对柚果总黄酮、柚皮苷、橙皮苷和野漆树苷有影响，为柚果合理利用提供参考价值. 相似文献
4.
以中华芦荟、库拉索芦荟和木立芦荟的叶片为外植体进行组织培养.结果表明三种芦荟均可以在特定的培养基中形成愈伤组织.中华芦荟愈伤组织的诱导最佳培养基为MS+6-BA0.2mg/L+2,4-D0.5mg/L;库拉索芦荟愈伤组织的诱导最佳培养基为MS+6-BA1.0mg/L+2,4-D1.0mg/L;木立芦荟愈伤组织的诱导最佳培养基为MS+6-BA2.0mg/L+2,4-D1.5mg/L. 相似文献
5.
以自交亲和芸芥7-5-1-1为研究材料,对影响芸芥花药愈伤组织诱导的主要因素进行了初步研究.结果表明:MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA+0.5 mg/L NAA是诱导芸芥花药愈伤组织适宜的培养基;芸芥花药在离体培养前最佳低温(4℃)预处理时间为2d,诱导率可高达46.2%;在诱导期间始终处于弱光或完全黑暗条件下的花药愈伤组织诱导率最高;单核期花药的愈伤组织诱导率最高,与单核前期、双核期花药的愈伤组织诱导率相比,差异达到极显著水平. 相似文献
6.
不同光质和培养基对毛地黄叶组织培养中强心苷积累的效应研究 总被引：4，自引：0，他引：4
通过对五种不同浓度培养基的不同光照处理，测定愈伤组织中强心苷的含量。结果表明：不同光质对毛地黄愈伤组织中强心苷的含量有明显的影响，蓝光、绿光、黄光对强心苷的产生和积累有促进作用，红光有抑制作用。在同一光质中，强心苷的含量与培养基中附加激素的浓度密切相关。 相似文献
7.
对沙田柚皮中柚皮苷提取工艺进行研究,通过单因素实验、正交实验等方法,确定了优化提取工艺:固液比为1∶10,乙醇浓度为70%,提取温度为40℃,提取时间为60min。本研究发现超声波辅助处理,不会显著增加柚皮苷提取率,但是可以在达到相同较高柚皮苷提取率的基础上,显著降低固液比,减少乙醇用量。 相似文献
8.
9.
10.
《河西学院学报》2017,(5):52-58
研究不同外植体、激素种类及其配比对黑果枸杞(Lycium ruthenicum Murr.)愈伤组织诱导和增殖的影响,建立优良的愈伤组织培养体系.结果表明,适合黑果枸杞愈伤组织诱导的最佳外植体是下胚轴,愈伤组织诱导适宜的培养基为MS+0.4mg·L~(-1)NAA+0.5mg·L~(-1)6-BA,诱导率达100%;增殖的适宜培养基是MS+0.4mg·L~(-1)6-BA+1.0mg·L~(-1)NAA+0.5mg·L~(-1)IAA,在此培养基上愈伤组织可连续增殖,在20d内增殖系数可达到3.22,且愈伤组织生长状态良好,质地松散,适合建立稳定高效的愈伤组织培养体系. 相似文献
11.
利用常规木材制片法,结合细胞图象分析系统及显微照相,对番石榴2个品种（土种、珍珠）茎次生木质部导管细胞结构进行了观察.结果表明：1、导管细胞均为具单穿孔板的孔纹导管;2、总体上可分为两端具尾、一端具尾和无尾3种类型的导管;3、土种、珍珠2个品种番石榴导管细胞平均长度分别为959.67±203.01μm、861.95±167.49μm,平均直径分别为210.54±64.72μm、192.59±57.97μm.番石榴不同品种导管细胞形态差异与其生长的差异具有一定的相关性. 相似文献
12.
通过在厦门地区对3个马来西亚番木瓜品种进行引种试验,结果表明,引种的马来10号和马来2号为中果型,植株均具有很好的结果性能,产量高,品质好,耐贮运,植株耐环斑花叶病.马来10号茎干、叶柄呈紫红色,节间距较短,较具矮生.2个品种均具有很好的推广应用前景.引种的蜜红3号为小果型,植株亦具有良好的结果性能,品质优,但产量较低,且存在果形较不稳定、畸形花果较多等问题,该品种不适宜推广栽培. 相似文献
13.
郑光宇 《喀什师范学院学报》2005,26(6):48-50
研究了西瓜花叶病毒-2新疆株（Watermelon mosaic virus-2-XJ,WMV-2-XJ）感染抗性不同的甜瓜品种后苯丙氨酸解氨酶和叶绿素含量的变化.与健康对照植株相比,受感染植株的酶活性均有所增高,但抗性品种“新玉”增高的幅度是感性品种“网纹香”的2倍,酶活性峰早出现12天,即抗性品种酶活性增高幅度大,酶活性峰出现早,叶绿素的变化则与抗性呈负相关,抗性品种“新玉”受WMV-2-XJ感染后的叶绿素含量与健康对照差异不大,而感性品种“网纹香”的叶绿素含量则比健康对照下降了62.9%.苯丙氨酸解氨酶和叶绿素含量的变化均与甜瓜品种对WMV-2-XJ的抗性呈现规律性的相关性,这种规律性可用于甜瓜品种对WMV-2-XJ 抗性鉴定的生物化学分析. 相似文献
14.
朱缨花愈伤组织的诱导 总被引：1，自引：0，他引：1
研究了不同外植体和不同生长调节剂对朱缨花愈伤组织诱导的影响．结果表明：不同外植体的愈伤组织诱导能力为茎段〉带节茎段〉叶柄〉叶片，茎段和带节茎段是愈伤组织诱导的最佳外植体；诱导朱缨花茎段愈伤组织的最佳生长调节剂组合为MS＋NAA1mg／L和MS＋BA0．5mg／L＋NAA1mg／L;诱导朱缨花带节茎段愈伤组织的最佳生长调节剂组合为MS＋BA2mg／L＋NAA1．5mg／L． 相似文献
15.
Hong Zhou Jin Liao Yi-ping Xia Yuan-wen Teng 《Journal of Zhejiang University. Science. B》2013,14(4):299-308
Evergreen azaleas are among the most important ornamental shrubs in China. Today, there are probably over 300 cultivars preserved in different nurseries, but with little information available on the cultivar itself or relationships between cultivars. Amplified fragment length polymorphism (AFLP) markers were employed to determine the genetic relationships between evergreen azalea cultivars in China. One hundred and thirty genotypes collected from gardens and nurseries, including cultivars classified in the groups East, West, Hairy, and Summer, unknown cultivars, and close species, were analyzed using three primer pairs. A total of 408 polymorphic fragments were generated by AFLP reactions with an average of 136 fragments per primer pair. The average values of expected heterozygosity and Shannon’s information index were 0.3395 and 0.5153, respectively. Genetic similarities were generated based on Dice coefficients, used to construct a neighbor joining tree, and bootstrapped for 100 replicates in Treecon V1.3b. Principal coordinate analysis (PCO) was performed based on Dice distances using NTSYS-pc software. The AFLP technique was useful for analyzing genetic diversity in evergreen azaleas. Cluster analysis revealed that cultivars in the West and Summer groups were quite distinct from other groups in the four-group classification system and that the East and Hairy groups should be redefined. 相似文献
16.
通过对本地主栽品种、外引品种与茹砧嫁接后进行比较试验，并根据当地消费食用习惯与茄子一大茬栽培要求，确定了茄王、兰杂二号为目前张掖市一大茬嫁接茄子最优品种；推荐日本长茄与张掖地方品种5叶茄的早春日光温室和大棚早熟栽培最适品种；各类图茄不适应本地消费习惯。 相似文献
17.
Soybean (Glycine max (L.) Merrill) is a salt-sensitive crop, and its production is severely affected by saline soils. Therefore, the response
of soybean seeds to salt stress during germination was investigated at both physiological and proteomic levels. The salt-tolerant
cultivar Lee68 and salt-sensitive cultivar N2899 were exposed to 100 mmol/L NaCl until radicle protrusion from the seed coat.
In both cultivars, the final germination percentage was not affected by salt, but the mean germination times of Lee68 and
N2899 were delayed by 0.3 and 1.0 d, respectively, compared with controls. In response to salt stress, the abscisic acid content
increased, and gibberellic acid (GA1+3) and isopentenyladenosine decreased. Indole-3-acetic acid increased in Lee68, but remained unchanged in N2899. The proteins
extracted from germinated seeds were separated using two-dimensional gel electrophoresis (2-DE), followed by Coomassie brilliant
blue G-250 staining. About 350 protein spots from 2-DE gels of pH range 3 to 10 and 650 spots from gels of pH range 4 to 7
were reproducibly resolved, of which 18 protein spots showed changes in abundance as a result of salt stress in both cultivars.
After matrix-assisted laser desorption ionization-time of flight-mass spectroscopy (MALDI-TOF-MS) analysis of the differentially
expressed proteins, the peptide mass fingerprint was searched against the soybean UniGene database and nine proteins were
successfully identified. Ferritin and 20S proteasome subunit β-6 were up-regulated in both cultivars. Glyceraldehyde 3-phosphate
dehydrogenase, glutathione S-transferase (GST) 9, GST 10, and seed maturation protein PM36 were down-regulated in Lee68 by salt, but still remained at
a certain level. However, these proteins were present in lower levels in control N2899 and were up-regulated under salt stress.
The results indicate that these proteins might have important roles in defense mechanisms against salt stress during soybean
seed germination. 相似文献
18.
Kaya M Kaya G Kaya MD Atak M Saglam S Khawar KM Ciftci CY 《Journal of Zhejiang University. Science. B》2008,9(5):371-377
Chickpea is an important food legume crop of Turkey and is largely grown for human consumption on low moisture or salt-affected soils.The objective of the study was to find the effects of NaCl stress at electrical conductivities of 4.5,8.6,12.7 and 16.3 dS/m and seed sizes (7,8 and 9 mm) on germination and early seedling growth of three popular chickpea cultivars (AKN-97,Gokce and Uzunlu-99).Mean frequency of germination,germination time,germination index,root length,shoot length and seedling fresh weight showed seed size-dependent responses of cultivars to salt stress.In general,small seeds germinated and grew more rapidly compared to medium and large seeds of the same cultivars against all levels of salt stress,with the best results in cultivar Uzunlu-99.No effect of NaCl treatments was observed on frequency of germination; however,a drastic decrease in early seedling growth was recorded at increased NaCl concentrations.Regression analysis results showed a significantly positive rela-tionship (P<0.01) between seed size and mean germination time,whereas a significantly negative relationship was recorded between seed size and germination index,root length,shoot length.Moreover,linear regression values apparently confirmed that increased seed size in each cultivar affected decreased germination index,root and shoot lengths with enhanced mean germination time.Thus,it was concluded that the use of small seeds could considerably reduce the production costs of chickpea in salt-affected soils. 相似文献
19.
An efficient induction system and regeneration protocol based on mature barley embryos were developed. Embryos isolated from
mature seeds, dehusked by hand and inoculated with longitudinally bisected sections, showed low contamination and high primary
callus-forming capability. The influences of nine culture media on primary callus induction and germination from the mature
embryos of barley cultivars Golden Promise and Zaoshu 3 were analyzed. The results showed that the two cultivars had much
higher values of primary callus induction in the B16M6D medium as compared to the other eight medium formulations, with a
frequency of 74.3% and 78.4% for Golden Promise and Zaoshu 3, respectively. Furthermore, Zaoshu 3 demonstrated particularly
high stability in callus induction over the different media, indicating its potential utilization in callus induction and
regeneration for its good agronomic traits and wide adaption. There were significant differences amongst 11 barley genotypes
in terms of primary callus induction in the optimum medium, with percentages of callus induction and germination response
ranging from 17.9% to 78.4% and 2.8% to 47.4%, respectively. Green plantlets of Dong 17, Golden Promise, and Zaoshu 3 were
successfully developed from primary calli through embryogenesis, with green plant differentiation frequencies ranging from
9.7% to 21.0% across genotypes. 相似文献
20.
渗透胁迫对玉米幼苗膜脂过氧化及过氧化氢酶活性的影响 总被引：6，自引：0，他引：6
本实验以三个玉米品种新实多2号、东单7号、中原单32为实验材料,采用PEG 6000模拟土壤干旱条件,研究渗透胁迫下不同玉米品种M DA(丙二醛)含量、CAT(过氧化氢酶)活性以及膜相对透性间的相互关系,结果表明,渗透胁迫下三个玉米品种M DA含量和质膜相对透性均随胁迫强度的增加而增加,而CAT活性则随胁迫强度的增加而减少,CAT活性与M DA含量、质膜相对透性均呈显著或极显著负相关关系,且品种间存在差异,通过分析比较,可初步判断三个玉米品种幼苗的抗渗透胁迫能力为新实多2号优于中原单32和东单7号. 相似文献