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1.
I. Rahman A. Upadhyay V. Tule N. Nath 《Indian journal of clinical biochemistry : IJCB》1993,8(1):29-32
We studied the membrane potentials of normal, essential hypertensive, diabetes mellitus and rheumatoid arthritic polymorphonuclear leukocytes (PMNL). It was found that diabetic PMNL were grossly hyperpolarized as compared to normal. On the other hand hypertensive and arthritic PMNL elicited a significant depolarization. The PMNL obtained from treated hypertensive patients showed considerable recovery where as partial recovery was evidenced in membrane potentials in diabetic and arthritic PMNL. The implications are discussed. 相似文献
2.
G. N. Raval L. J. Parekh D. D. Patel F. P. Jha R. N. Sainger P. S. Patel 《Indian journal of clinical biochemistry : IJCB》2004,19(2):60-71
Sialic acid, the end moieties of the carbohydrate chains are biologically important and essential for functions of glycoconjugates
and are reported to be altered in cancer patients. Two hundred and twenty five breast cancer (BC) patients, 100 patients with
benign breast disease (BBD) and 100 healthy females (controls) were enrolled for the study. Eight hundred and twenty four
follow-up samples of 225 breast carcinoma patients were also evaluated. The association of sialic acid forms, sialyltransferase
and α-2–6 sialoproteins levels with presence and extent as well as prognosis of breast carcinoma was studied. Serum sialic
acid forms and sialyltransferase revealed significantly elevated levels among untreated breast cancer patients as compared
to the controls, patients with BBD as well as cancer patients in remission. Non-responders showed comparable levels of the
markers with those found in breast cancer patients at the time of diagnosis. Higher levels of sialic acid forms at diagnosis
were associated with poor prognosis. A positive correlation between serum levels of different forms of sialic acids and extent
of malignant disease was observed. The changes in serum proteins with terminal α-2–6 sialic acid correlated well with alterations
in the levels of sialic acid forms and sialyltransferase. Malignant tissues showed elevated levels of sialic acid and sialyltransferase
as compared to surrounding normal tissues.
The results suggested potential utility of these markers in evaluation of clinical outcome. 相似文献
3.
H. R. Mali M. L. B. Bhatt M. P. Singh S. M. Natu J. P. Gupta 《Indian journal of clinical biochemistry : IJCB》1996,11(1):56-58
Serum sialic acid levels were determined in 66 patients of carcinoma cervix and 25 control women matched for age. Mean sialic acid level was significantly elevated (p<0.001) in cancer cervix patients as compared to controls. Patients were treated with 50–65 Gy of radiation in 5–6.5 weeks. Post-therapy levels were again determined. Radiotherapy caused decline in serum sialic acid level in all the patients except one. Mean post-therapy level was significantly lower as compared to pretreatment level (p<0.001). It was also observed that fall in sialic acid level was significantly higher (p<0.05) in early stage patients as compared to late stage. No relationship was observed in the dose of radiation and fall in serum sialic acid level. A significantly higher fall in serum sialic acid level was observed in patients having complete clinical response as compared to partial or nonresponders to radiation treatment (p<0.001). 相似文献
4.
P. K. Nigam V. S. Narain Ajay Kumar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):54-61
Sialic acid, the acylated derivatives of 9-carbon sugar neuraminic acid, present as terminal component of oligosaccharide
chains of many glycoproteins and glycolipids, has been recognized to be involved in the regulation of a great variety of biological
phenomena. Studies have shown that serum sialic acid predicts both coronary heart disease and stroke mortality and reflects
the existence or activity of an atherosclerotic process. Most of the studies have shown an elevation in serum sialic acid
concentration in coronary heart disease and a positive correlation between the raised serum sialic acid and the severity of
the coronary lesions is observed. However, a few contradictory reports are also available. Racial differences in serum sialic
acid have also been reported and correlated with international differences in the prevalence of atherosclerosis. Reduced sialic
acid content of platelets, erythrocytes and lipoproteins may play important role in the pathogenesis of atherosclerosis. Elucidation
of the mechanism of alternation in sialic acid concentration may throw more light on its potential clinical utility. Hence
more studies are needed to designates sialic acid as a cardiovascular risk factor/marker. 相似文献
5.
C. R. Wilma Delphine Silvia D. M. Vasudevan K. Sudhakar Prabhu 《Indian journal of clinical biochemistry : IJCB》2001,16(1):113-115
The present study was conducted on thirty untreated oral cancer patients proved by clinical and histopathological evidence
and thirty healthy control subjects. The levels of glycoprotein-associated carbohydrates such as hexose, hexosamine, fucose
and sialic acid were found to be elevated significantly as compared to control subjects. There was a progressive rise in these
markers as the stages of oral cancer advanced. 相似文献
6.
P. K. Nigam V. S. Narain Naveen Chandra V. K. Puri R. K. Saran S. K. Dwivedi M. Hasan 《Indian journal of clinical biochemistry : IJCB》1995,10(2):106-109
Blood samples from 39 patients with acute myocardial infarction and 15 healthy controls were analysed for serum and platelet sialic acid. Serum sialic acid levels in patients with acute myocardial infarction were significantly higher than controls (mean 2.7±0.46 μmol/ml Vs. 1.91±0.17 μmol/ml respectively). Levels of serum orosomucoid, an acute phase reactant, containing sialic acid, were also higher in these patients, suggesting a possible non-specific mechanism of increase in serum sialic acid concentration. In contrast, platelets contained significantly less sialic acid in patients with acute myocardial infarction than control (26.73±1.57 nmol/mg protein and 31.97±2.68 nmol/mg protein respectively). 相似文献
7.
Sharmila Upadhya Subramanya Upadhya K. S. Prabhu 《Indian journal of clinical biochemistry : IJCB》2002,17(1):20-24
The present study was done to assess the levels of glycoconjugates and ceruloplasmin in sera of patients with cervical cancer.
Serum hexoses, hexosamines, sialic acid and fucose are elevated in a variety of inflammatory and neoplastic conditions. All
the glycoconjugates, except fucose were increased in serum of patients compared to controls. Also, hexoses and sialic acid
levels were high in patients with later stages of cancer compared to patients with early stage disease (P=<0.0001, P=0.03).
Serum ceruloplasmin was increased in patients with early stage cancer (51.5mg/dl) and with late stage cancer (61mg/dl) compared
to controls (38mg/dl). The elevated glycoconjugates may be the result of inflammatory reaction associated with neoplasia,
as serum ceruloplasmin (an acute phase reactant) is also increased in these patients. 相似文献
8.
Miriam Varkey R. Sundara Devi Shrinivas B. Rao 《Indian journal of clinical biochemistry : IJCB》1997,12(1):63-66
The levels of total sialic acid, lipid bound sialic acid and fucose were estimated in the sera of patients with benign and
malignant tumours of breast. An increase was noted in all the three parameters, with a more marked increase in malignancy,
when compared with benign and controls. Consequent to surgery, there was an elevation in the serum levels of the above parameters
than the values prior to surgery and a decline was noted two months after surgery although none of the values reached the
normal range. These results suggest a close association of the glycoproteins with the tumour burden and further signify their
role in early detection and staging of cancer breast. 相似文献
9.
Gauri S. Vahalkar Vijaya A. Haldankar 《Indian journal of clinical biochemistry : IJCB》2008,23(3):223-226
Glyco-oxidation is considered as a source of permanent, cumulative, oxidative damage to long lived proteins in ageing and
in diabetes. Although RBC depends solely on glucose for energy purpose, hyperglycemic state glycosylates hemoglobin, creates
oxidative stress and puts the cellular components at risk. In view of this, RBC membrane composition was analyzed in diabetic
patients. The results were compared with healthy age and sex matched control groups. When RBC membrane components such as
protein, sialic acid, phospholipids and cholesterol were determined in insulin dependent diabetes mellitus, a significant
rise in phospholipids and cholesterol and significant fall in sialic acid and protein content was noted. RBC membrane composition
showed pronounced alterations in insulin dependent diabetes mellitus. These changes were accompanied by higher levels of lipid
peroxidation products like Malondialdehyde. 相似文献
10.
S. Biswas A. Bhelwa A. Ittoop S. Biswal N. Nath 《Indian journal of clinical biochemistry : IJCB》1994,9(2):75-78
Normal polymorpho nuclear leucocytes (PMNL) when assaulted with diabetic serum factorin-vitro, recorded enhanced generation of oxygen free radical, and lysosomal degranulation. It also caused a decrease in the cytosolic
superoxide dismutase activity. Time-plot studies revealed PMNL susceptibility to diabetic serum factor for oxidative injury
and concomitant decrease in SOD, consequential for lysosomal degranulation. 相似文献
11.
Praveen Kumar Shetty T. N. Pattabiraman 《Indian journal of clinical biochemistry : IJCB》2004,19(1):97-101
The present study deals with the estimation of total carbohydrate, protein bound carbohydrate, bound fucose and sialic acid
along with total protein in disease conditions like gingivitis, periodontitis and their comparison with the normals.
The neutral hexose values in gingivitis (8.08±2.20mg/100mg protein) and periodontitis (12.5±2.16mg/ 100mg protein) decreased
significantly when expressed per 100 mg protein compared to normals (19.8±1.89mg/100mg protein). This might be because of
higher protein concentration in these two clinical conditions. The ethanol insoluble hexose values were significantly reduced
in both these conditions compared to controls (3.71±1.64,5.91±1.63,7.65±0.86mg/100mg protein respectively). The ethanol soluble
hexose values were also found to be drastically reduced. This decrease in saliva appears to be characteristic feature of oral
diseases. In gingivitis and periodontitis fucose level was found to be increased compared to normals when expressed as a function
of salivary volume. However in terms of protein concentration the values in gingivitis (2.95±1.59), periodontitis (3.26±0.98)
and normals (3.20±0.50mg/100mg) were not different. Sialic acid in ethanol insoluble fraction of salivary samples mg/100mg
protein was found to be significantly reduced in both gingivitis (0.78±0.33) and periodontitis (0.95±0.31) compared to controls
(1.92±0.33) 相似文献
12.
Srinivasa Nageswara Rao G Prema G Priya G Arumugam SB Kirthivasan V Saibabu R Cherian KM 《Indian journal of clinical biochemistry : IJCB》2011,26(1):22-27
There is a rising trend in the prevalence of insulin resistance among obese, overweight children and adolescents. The serum
insulin and its correlation with biochemical, clinical and anthropometric parameters were evaluated in 185 children and adolescents
(59 control, 52 obese, 49 overweight, 25 congenital heart disease) of age group 10–17 years. The levels of serum insulin were
measured by ELISA. Serum insulin levels were found to be significantly increased in children who were obese, overweight and
had congenital heart disease, than controls. Serum insulin levels positively correlated with BMI, WHR, and serum C-peptide,
serum leptin, total cholesterol, triglycerides, LDL-cholesterol, systolic and diastolic blood pressure. Fasting glucose levels
were found to be negatively correlated with serum insulin levels. HDL-cholesterol levels were non-significant among the study
groups. We identified nine obese children (five girls and four boys) with the features of metabolic syndrome and 69% of obese
and overweight children were identified with insulin resistance. Insulin resistance was strongly associated with metabolic
syndrome and its components, especially with central obesity and hypertriglyceridemia. 相似文献
13.
S. P. Kulkarni C. R. Mallikarjuna D. S. Jayaprakash Murthy 《Indian journal of clinical biochemistry : IJCB》2006,21(1):185-188
Background Sialic acid (SA) is a nine carbon sugar derived from mannosamine and pyruvate. High levels of sialic acid and aspartate transaminase
(AST) levels in cerebrospinal fluid have been described in pyogenic meningitis (PM) compared to tubercular meningitis (TBM).
Objectives To evaluate the levels of CSF free SA in PM and TBM and to assess the correlation between CSF free SA and CSF glucose or total
protein levels.
Patients and Methods A total of 122 subjects were studied and divided into children and adults. Further, these have been subdivided into controls,
PM and TBM. CSF free SA was estimated by thiobarbituric acid assay of Warren and AST by Reitmann and Frankel method.
Results CSF free SA and AST levels in children and adults were significantly high in PM (p<0.001) as compared to TBM and controls.
Conclusion A very high CSF free SA and AST were found to be characteristic of PM, making them useful parameters to differentiate PM from
TBM. 相似文献
14.
Vikram Thakkar Purvi Patel Neelam Prajapati Ranjit Kaur Mukesh Nandave 《Indian journal of clinical biochemistry : IJCB》2014,29(3):345-350
Identification of reliable biomarkers for detection and staging of cancer and monitoring the outcome of anticancer therapy has been considered to be of high importance. We aimed to estimate the levels of serum glycoproteins, protein bound-hexose, protein bound hexosamine, protein bound fucose, protein bound sialic acid and protein bound carbohydrate in 32 ovarian cancer patients and compared them with the levels that found in 25 normal subjects. As compared to the normal subjects, all the four fractions of glycoproteins level were significantly elevated in ovarian cancer patients (p < 0.05). Chemotherapy in these patients significantly decreased the levels of serum glycoproteins (p < 0.05). Thus, high levels of serum glycoproteins in ovarian cancer patients could be due to abnormal protein glycosylation indicating malignant transformation of the cells. 相似文献
15.
Sunil Kumar Gupta R. C. Gupta Kapil Gupta H. P. Trivedi 《Indian journal of clinical biochemistry : IJCB》2008,23(2):176-180
This study was conducted to find out the possible underlying mechanism of various manifestation of fluorosis, a disease caused
by excess ingestion of fluoride. For this the fluoride belt of Jaipur district was selected. The parameters selected were
serum Parathyroid hormone, the levels of which are directly affected by fluoride intake. The levels of serum seromucoid, serum
and leucocyte ascorbic acid, serum sialic acid (SSA) reflects ground substance metabolism. The study was conducted on two
hundred children, selected from four areas (50 from each area) consuming water containing 2.4, 4.6, 5.6 and 13.6 mg/l of fluoride.
Drinking water fluoride and serum fluoride were measured by Ion selective electrode method. Serum parathyroid by RIA and all
other parameters were measured spectrophotometrically. The results revealed an increase in levels of fluoride, parathyroid
hormone and seromucoid in serum with increasing water fluoride concentrations. Serum Calcium and serum ascorbic acid were
found in normal range, how ever leucocyte ascorbic acid were decreased. A high positive correlation among fluoride concentration
in drinking water and serum parathyroid hormone (r=0.967), and, serum parathyroid hormone and serum seromucoid concentration
(r=0.935) was also observed The results indicated that secondary hyperparathyroidism due to hypocalcemic stress caused by
excess fluoride ingestion disturbs normal metabolism of ground substance in calcified tissues of the body reflected as altered
levels of the components of ground substance in the serum. 相似文献
16.
Aarzoo Juneja Shridhar Dwivedi D. K. Srivastava Kailash Chandra 《Indian journal of clinical biochemistry : IJCB》2017,32(1):99-102
Insulin resistance is a condition in which cells fail to respond to the normal actions of insulin. Dietary fat, obesity and smoking have been attributed to increase insulin resistance. However, the prevalence of insulin resistance in young obese subjects and its relation to smoking is not well established. This study comprising seventy-five healthy young adults was undertaken to find insulin resistance in obese smokers and non smokers both. Present study showed an overall prevalence of raised homeostatic model assessment of insulin resistance in 14.7 % otherwise healthy young subjects (20–30 years age group). Non-smokers did not show any significant correlation between insulin resistance and body mass index at either stage (normal, pre-obese as well as obese). Smokers also did not show any significant difference of insulin resistance in normal and pre-obese stages. However, marked increase in homeostatic model assessment of insulin resistance was observed in obese smokers. Homeostatic model assessment of insulin resistance showed a linear trend in relation to body mass index and its values were found to be higher in smokers. Obesity combined with smoking demonstrated statistically significant increase in homeostatic model assessment of insulin resistance. 相似文献
17.
Bina R. Baxi Prabhudas S. Patel Siddharth G. Adhvaryu 《Indian journal of clinical biochemistry : IJCB》1990,5(2):139-144
For evolving a potential Biochemical index for early diagnosis of oral cancer, serum levels of total sialic acid (TSA), lipid
bound sialic acid (LSA), mucoid proteins and hexoses (galactose and mannose) were determined in 30 patients with oral cancer.
The results were compared with similar values obtained for 30 patients with oral precancerous conditions, 24 healthy controls
and 15 normal individuals with a habit of tobacco chewing. All the biomarkers were significantly elevated in oral cancer patients
compared to the controls, the normal chewers and precancer patients. The levels of glycoconjugates showed significant increase
in patients with oral precancerous conditions compared to the controls. Only LSA and hexoses showed significantly elevated
levels in oral precancer patients compared to the normal chewers. The levels of glycoconjugates were more or less comparable
between normal chewers and the controls. The parameters evaluated in the oral cancer patients also showed a progressive rise
with the clinical stage of the malignant disease. The levels of the four glycoconjugates were also higher in patients with
metastasis compared to those with only primary disease. The present findings suggest that the glycoconjugates have clinical
utility in diagnosis of the patients with cancer of the oral cavity as well as in staging the disease. 相似文献
18.
J. S. Suresh Kumar P. Menon Venugopal 《Indian journal of clinical biochemistry : IJCB》1992,7(2):109-113
Changes in the levels of glycoprotein (GP) and glycosminoglycans (GAG) were studied in diabetes associated with myocardial infarction in rats. Diabetes was induced by alloxan while myocardial infarction was induced using isoproterenol. Most of the GAG fraction increased in the heart and aorta in diabetes superimposed with myocardial infarction. The carbohydrate of GP also increased in heart while in serum only sialic acid showed an increase. The activity of lysosomal enzymes showed an increase in the case of β-glucuronidase and β-hexosaminidase while in the case of β-galactosidase and β-fucosidase it showed a decrease in the heart. 相似文献
19.
BackgroundDiabetes is a metabolic disorder caused by defects in insulin production and activity. During disease progression, changes in lipid peroxidation cause structural modifications via production of free radicals. Fangchinoline is a well-known alkaloid present in Stephaniae tetrandrine S. Moore, which has demonstrated antioxidant, anticancer, and anti-inflammatory activities.ResultsThe present study analyzed the anti-diabetic and antioxidant effects of fangchinoline in male rats with streptozotocin-induced diabetes. Rats were divided into the following groups: normal control, diabetic, diabetic + fangchinoline 100 mg/kg, diabetic + fangchinoline 200 mg/kg and diabetic + glibenclamide 600 µg/kg. The treatment was administered orally for 45 consecutive days. Lipid peroxidation was substantially increased by >50% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. However, fangchinoline supplementation significantly reduced lipid peroxidation to near normal levels. Reactive oxygen species levels were substantially increased by >500% in the serum, as well as the liver, kidney, and heart tissues of diabetic rats. Fangchinoline supplementation reduced reactive oxygen species to near normal levels. Fangchinoline supplementation significantly improved superoxide dismutase, glutathione peroxidase, catalase, and reduced glutathione levels in diabetic rats. Total hexoses, sialic acid, hexosamines, and fucose were increased in diabetic rats, whereas fangchinoline supplementation significantly reduced these total hexoses, sialic acid, hexosamines, and fucose to near normal levelsConclusionsSupplementation with fangchinoline led to significant attenuation of the levels of lipid peroxidation, ROS, and glycoprotein components such as total hexoses, hexosamines, sialic acid, and fucose, while improving antioxidant marker levels.How to cite: Xia J, Huang W, Zhou F. Effect of fangchinoline on oxidant status in male albino rats with streptozotocin-induced diabetes. Electron J Biotechnol 2021;53. https://doi.org/10.1016/j.ejbt.2021.07.005 相似文献
20.
Farzana Bano A. M. Kidwai R. K. Upreti R. K. Singh 《Indian journal of clinical biochemistry : IJCB》1994,9(2):109-111
The present studies with aluminium chloride given in drinking water showed marked inhibition in the activities of intestinal brush border membrane marker enzymes, namely alkaline phosphatase, acetyl cholinesterase, γ-glutamyl transpeptidase and sucrase. Moreover, a significant depression in the levels of membrane constituents, viz phospholipids, cholesterol, hexoses and sialic acid following aluminium chloride treatment was also observed. On the basis of these studies it may be concluded that if aluminium is taken in high quantities it may alter the structure and functioning of the intestinal brush border membrane, which in turn may lead to the improper digestion and reduced nutrient uptake from the small intestine. 相似文献