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1.
Assignment of <Emphasis Type="Italic">CCR7</Emphasis> gene to chicken chromosome 27 by radiation hybrid panel mapping 总被引：1，自引：0，他引：1
Tian Y Lu LZ Fu Y Tao ZR Shen JD Wang DQ Yuan AP Yin ZZ 《Journal of Zhejiang University. Science. B》2007,8(5):314-317
The protein encoded by CC chemokine receptor 7 （CCR7） is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus （EBV）, and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. To map the CCR7 gene in chicken chromosome, a 6000 rads chicken-hamster radiation hybrid panel （ChickRH6） was used. PCR of samples from ChickRH6 revealed that the location of CCR7 gene is linked to the maker SEQ0347 （6 cR away） with LOD score of 16.6 and that the marker SEQ0347 is located on chromosome 27 at 27 cR of RH （radiation hydrid） map. We compared the corresponding human mRNA sequence with the predicted coding sequence of chicken CCR7 gene, and found that the assembled contig shared a high percentage of similarity with that of the human gene. 相似文献
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Transfection and expression of exogenous gene in laying hens oviduct in vitro and in vivo 总被引：2，自引：0，他引：2
Gao B Sun HC Song CY Wang ZY Chen Q Song HQ 《Journal of Zhejiang University. Science. B》2005,6(2):137-141
To examine whether or not the regulatory sequence of chicken ovalbumin gene can drive transgene expression specifically in hen oviduct, the authors constructed an oviduct-specific expression vector (pOV), containing 3.0 kilobases (kb) of the 5'-flanking sequence and 3.0 kb of the 3'-flanking sequence of the chicken 相似文献
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根据GenBank发表的鸡γ-干扰素核苷酸序列,使用primer 5设计一对特异性引物,通过RT-PCR技术从ConA诱导培养的鸡脾脏淋巴细胞中克隆出鸡γ-干扰素基因并对其进行测序,测序结果表明,鸡γ-干扰素基因全长495bp,具有一个完整的开放阅读框,编码164个氨基酸,与国外发表的序列比较,两序列间同源性为100%.计算机软件对鸡γ-干扰素编码的氨基酸序列进行了抗原性分析,结果表明鸡γ-干扰素具有良好的免疫原性. 相似文献
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以30例胃癌组织和正常组织为研究对象,通过STR-PCR技术,对6号染色体6q24内的ZAC基因两侧的4个STR基因座进行杂合性缺失分析,统计这4个STR基因座在30例标本中的LOH频率,绘制它们的缺失图谱,得到了第4号和25号这两个样本中发生了包含ZAC基因在内的4285651bp的缺失,为进一步分析ZAC基因发生缺失的断裂点和查找ZAC基因表达下调的原因打下基础. 相似文献
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Epstein-Barr virus(EBV),a human gammaherpesvirus carried by more than 90% of the world’s population,is associated with malignant tumors such as Burkitt’s lymphoma(BL),Hodgkin lymphoma,post-transplant lymphoma,extra-nodal natural killer/T cell lymphoma,and nasopharyngeal and gastric carcinomas in immune-compromised patients.In the process of infection,EBV faces challenges:the host cell environment is harsh,and the survival and apoptosis of host cells are precisely regulated.Only when host cells receive sufficient survival signals may they immortalize.To establish efficiently a lytic or long-term latent infection,EBV must escape the host cell immunologic mechanism and resist host cell apoptosis by interfering with multiple signaling pathways.This review details the apoptotic pathway disrupted by EBV in EBV-infected cells and describes the interactions of EBV gene products with host cellular factors as well as the function of these factors,which decide the fate of the host cell.The relationships between other EBV-encoded genes and proteins of the B-cell leukemia/lymphoma(Bcl) family are unknown.Still,EBV seems to contribute to establishing its own latency and the formation of tumors by modifying events that impact cell survival and proliferation as well as the immune response of the infected host.We discuss potential therapeutic drugs to provide a foundation for further studies of tumor pathogenesis aimed at exploiting novel therapeutic strategies for EBV-associated diseases. 相似文献
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在人体内,CCR5与许多免疫疾病有关,CCR5有望成为众多药物的作用靶点。将ccr5基因与真核表达载体pBBS242构建成重组质粒pBBS242-ccr5,转染CHO细胞,并经潮霉素B筛选。流式细胞仪检测结果表明CCR5在CHO细胞得到了稳定表达。 相似文献
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人类STR标记的研究进展 总被引：4，自引：0，他引：4
STR是由1～6个核苷酸的串联重复片段构成的,均匀分布于人类基因组中的简单重复序列,由于重复单位的重复次数在个体间呈高度变异性并且数量丰富、分布广且均匀、多态信息含量高、检测快速方便等特点,因此目前被广泛应用于人类基因定位、连锁分析、血缘关系鉴定、群体遗传学、系统发生树构建、遗传做图等方面. 相似文献
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A fiberless seed mutant (fl) was identified in a commercial cotton (Gossypium hirsutum L.) variety Xu-Zhou 142 (FL). This phenotype is associated with lack of fiber cell initiation in the outer integument of
the ovule, as was characterized by analysis of genes related to fiber differentiation and development. Two genes, fl-E6 and
FL-E6, were cloned from fl-integument cells and FL-fiber or integument cells, respectively. Compared with FL-E6, fl-E6 showed
a dramatic change in nucleotide sequence: (1) FL-E6 contained a tandem repetitive sequence in which GGCTCA (Gly-Ser) is repeated
five times between the 82nd and the 93rd codon from the first ATG codon, while in fl-E6 the same sequence is repeated four
times; (2) The fl-E6 gene encodes a polypeptide of 241 amino acids but lacks two codons between the 90th and 93rd codon and
three between the 171st and 174th relative to FL-E6; (3) There are also 12 nucleotide substitutions which would result in
7 amino acid differences between fl-E6 and FL-E6. Analysis of RT-PCR and Northerm Blot showed that expression of the fl-E6
gene is suppressed in the fl-integument cells, but highly expressed in FL-fiber cells. The difference between fl-E6 and FL-E6
may be associated with lower expression of fl-E6 in the fl-integument cells. Searches of protein databases with the FL-E6
gene sequence showed similarity to the protein backbones of two arabinogalactan-proteins (AGPs), one from the filtrate of
suspension-cultured cells ofPyrus cornmunis (AGPPc2) and the other fromNicotiana alata (AGPNa2). Although the function of the FL-E6 protein in differentiation and development of cotton fiber cells is not known,
the data indicate that the mutation of fl-E6 gene from FL-E6 gene may inhibit the fiber cell initiation from epidermal cells
of the outer integument of the ovule.
Project supported by the National Natural Science Foundation of China (Nos. 39770473 and 30170058), National Program of Plant
Gene Transfer (J00-B-002-10), National High Technology Research and Development (863) Program of China (No. 2001AA212081),
and Research Program of Ministry of Education of China (No. 0114) 相似文献
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In the search for a rapid and reliable method for identification of bacteria in blood and cerebrospinal fluid , we developed a unified set of primers and used them under polymerase chain reaction(PCR) to amplify the spacer regions between the 16s and 23s genes in the prokaryotic rRNA genetic loci . Spacer regions within these loci showed a significant level of length and sequence polymorphism across most of the species lines. A generic pair of priming sequences was selected from highly conserved sequences in the 16s and 23s genes occurring adjacent to these polymorphic regions. This single set of primers and reaction conditions were used for the amplification of the 16s-23s spacer regions for 61 strains of standard bacteria and corresponding clinical isolates belonging to 20 genera and 27 species, including Listeria, Staphylococcus and Salmonella species, et al. When the spacer amplification products were resolved by electrophoresis, the resulting patterns could be used to distinguish most of the bacteria species within the test group, and the amplification products of the clinical isolates clustered at the standard species level. Some species presenting similar pattern were further analyzed by HinfI or AluI digestion or DNA clone and sequences analysis in order to establish the specific 16s-23s rRNA gene spacer regions map. Analysis of 42 blood specimens from septicemic neonates and 6 CSF specimens from suspected purulent meningitis patients by bacterial culture and PCR-RFLP(Restriction Fregament Length Polymorphism) showed that 15 specimens of blood culture were positive(35.7%) in the 42 septicemic neonates; 27 specimens were positive(64.2%) by PCR, and that the positive rate by PCR was significantly higher than that by blood culture(P<0.01). Among the 6 CSF specimens, one specimen found positive by blood culture was also positive by PCR, two found negative by blood culture showed positive by PCR; all three were S.epidermidis according to the DNA map. One C.neoformans found positive by blood culture showed negative by PCR. The remaining two specimens were both negative by PCR and blood culture. These results indicated that the method of detecting bacterial 16s-23s rRNA spacer regions using PCR and RFLP techniques was rapid, sensitive and specific in the detection of bacterial infections; and so, has very important application in the clinical diagnosis of sepsis in neonates. 相似文献
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Plastids of nongreen tissues import carbon as a source of biosynthetic pathways and energy, and glucose 6-phosphate is the
preferred hexose phosphate taken up by nongreen plastids. A cDNA clone encoding glucose 6-phosphate/phosphate translocator
(GPT) was isolated from a cDNA library of immature seeds of rice and named asOsGPT. The cDNA has one uninterrupted open reading frame encoding a 42 kDa polypeptide possessing transit peptide consisting of
70 amino acid residues. TheOsGPT gene maps on chromosome 8 of rice and is linked to the quantitative trait locus for 1000-grain weight. The expression ofOsGPT is mainly restricted to heterotrophic tissues. These results suggest that glucose 6-phosphate imported viaGPT can be used for starch biosynthesis in rice nongreen plastids.
Project supported by National Natural Scienc Foundation of China (No.39830250) and Natural Science Foundation of Zhejiang
Province (No.2A0106), China. The nucleotide sequence data will appear in the GenBank under accession number AF375053. 相似文献
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本实验采用乳糖发酵短杆菌B_(27-12)作为出发菌株进行试验,首先,通过噬菌体敏感性试验,证明B_(27-12)对天津短杆菌T_(6-13)的五种噬菌体不敏感,从而证明B_(27-12)与T_(6-13)是不同噬菌体类型的谷氨酸生产菌。然后,将B_(27-12)用诱变效率高的原生质体诱变方法进行诱变选育,得到产酸较高的突变株,再进一步通过硫酸二酯(DES)和紫外线复合诱变法进行诱变,选育得到一株产酸较高的突变株D_(16)。 相似文献
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用鸡源新城疫病毒凤阳分离株WF00C对9～10 日龄SPF鸡胚的尿囊腔接种,成功增殖了该病毒,采用一步法RT-PCR技术扩增WF00C病毒的F基因,获得了1条1.7kb的特异性条带.用PCR产物直接测序.测序结果表明,扩增片段大小为1782bp,含有1个1662bp的开放性阅读框,编码554个氨基酸.核苷酸同源性分析表明:WF00C株与国内外其他NDV F基因的同源性为84.8%～97.5%,其中与国内标准强毒株F48E9的同源性为87.1%,说明WF00C与国内外的传统毒株有较大变异.与Taiwan95株和J株的同源性为94.1%和97.5%,说明WF00C与Taiwan95株和J株亲缘关系较近,具有较高的相似性.F蛋白裂解位点的氨基酸顺序为112Arg-Arg-Gln-Lys -Arg-Phe117, 表明为NDV的强毒株.蛋白疏水性和抗原性分析表明与标准强毒株相比没有太大的变异. 相似文献
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The scope of the present study was first to evaluate the cross‐cultural reliability and validity of the Social Emotional Questionnaire (SEQ) and second to estimate and compare the prevalence rates of childhood developmental and psychiatric disorders in the general population of young children in the Netherlands and Greece. To this end, the caregivers of 1748 Dutch and 384 Greek 4–12‐year‐old children from the general population completed the SEQ. The number of children displaying symptoms of childhood developmental disorders was estimated by applying the Diagnostic and Statistical Manual of Mental Disorders‐IV criteria of symptom occurrence. Results showed that the reliability and the construct validity of the SEQ were acceptable in both countries and for all the age‐groups of children. Concerning the prevalence, the Greek children were found to display overall significantly more symptoms of developmental disorders than the Dutch children. However, when the number of children suffering from psychiatric symptoms in the clinical range was estimated using the clinical criteria provided by categorical classification systems, no statistical significant differences emerged between the two countries. This finding suggests that when the criterion of clinical impairment is applied in diagnostic procedures, the number of children suffering from severe psychiatric disorders is about equal in the two countries. The implications of the study are discussed. 相似文献
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生肌决定因子 MyoD 是生肌调节因子 MRFs 家族的一个重要成员,在基因转录调控中起着重要的作用.本实验通过 RT-PCR 和3’-RACE 等方法,以松江鲈肌肉总 RNA 为模板,扩增出957 bp 的 MyoD 基因部分序列,包含部分编码区长567 bp,编码翻译188个氨基酸残基,3’-UTR 区长390 bp.系统发育分析表明,松江鲈 MyoD 与奥尼罗非鱼亲缘关系最近.不同组织半定量分析显示,松江鲈 MyoD 在各种组织中均有表达,在心脏中表达量最高,预示着在不同组织中该基因的功能广泛 相似文献
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应用免疫组织化学PAP法及多种单克隆抗体,对12例常见消化系统恶性肿瘤进行了原位研究。结果显示:①TIL在肿瘤局部主要分布于癌间质区,而癌实质区仅有极少数TIL浸润;②TIL中的主导细胞为CD_3~ T细胞,且无论在实质区还是在间质区CD_4~ 细胞均少于CD_8~ ,癌间质区CD_4~ /CD_8~ 比值大于癌实质区;③无论在癌实质区还是在癌间质区,均有部分CD_(25)~ 或HLA-DR~ TIL存在,对于这些变化的内在机制文中也作了探讨。 相似文献