共查询到19条相似文献，搜索用时 734 毫秒
1.
辛春艳 《河北北方学院学报(医学版)》1992,(2)
本文接血型常规检验法检测了380名(男166人,女214人)河北籍汉族大学生的ABO血型。用X~2方法对男女及河北地区与兰州、湖北地区血型分布进行了统计学分析,并根据Hardyweiberg定律计算了IA、IB、i的基因频率,结果表明:河北地区汉族人群血型分布B>A>O>AB,男女间无显著差异;河北地区分别与兰州、湖北地区相比,河北地区与湖北地区在B型和O型血的分布存在极显著性差异(P<0.01);河北地区汉族人群IA、IB、i的基因频率分别为:0.2251,0.2722,0.5027.这些结果为河北地区群体遗传学的研究提供了参数。 相似文献
2.
目的:研究与胰岛素抵抗有关的三个基因的多态性GPR10(G-protein-coupled re-ceptor 10)(-62 G/A)、FOXC2(Forkhead box C2)(-512 C/T)、PGC1(Peroxisome proliferatoractivated receptorγcoactivator-1)(Thr394Thr)与2型糖尿病相关性.方法:利用等位基因专一性PCR的方法,对来自上海地区的正常人和肥胖的2型糖尿病患者进行基因分型,并通过X2比较基因型频率及等位基因频率在两组人群中是否存在显著性差异.结果:GPR10基因-62G/A多态性基因型频率在糖尿病人群和正常人群中的分布分别为:糖尿病人AA 14、AG 39、GG 34;正常人AA 2、AG 44、GG 42,具有显著性差异,等位基因频率分布为:糖尿病人A 67 G107、正常人A48、G 128,也有显著性差异.结论:GPR10基因-62G/A多态性可能与2型糖尿病相关,但该结果仍有待于进一步扩大样本量进行验证. 相似文献
3.
目的研究山东地区汉族人群4个STR位点（D10S1248、D14S1434、D22S1045、SE33）的遗传多态性.方法运用PCR扩增、8%非变性聚丙烯酰胺凝胶电泳结合银染技术对152个无关汉族人群个体进行多态性研究.结果4个位点分别检测出72、80、72、136个等位基因片段,共有53个基因型,其频率分布在0.0526~0.1579之间,经检测其多态性分布符合Hardy-weinberg平衡定律.4个STR位点多态信息量（Polymorphism information content,PIC）分别为0.8493、0.8785、0.8173、0.9157.期望杂合度（Heterozygosity,HET）分别为0.8643、0.8892、0.8374、0.9211.结论山东地区汉族该4个STR基因座位点的多态性数据显示其基因分布特征具有特异性. 相似文献
4.
华芳 《泰州职业技术学院学报》2007,7(3):71-74
用A表示在E={z：｜z｜〈1）内解析，具有形式f（z）=z＋^∞∑n=2 anzn的全体函数组成的类。当f∈A时,记S^＊（γ）,C（γ）,K（β,γ）,K^＊（β,γ）为γ阶星象函数，γ阶凸象函数，γ型β阶近于凸函数，γ型β阶拟凸函数类，0≤β〈1,0≤γ〈1.用算子D^α刻划上述四个函数类的新子类Sα^＊（γ）,Ca（γ）,Kα（β,γ）,Kα^＊（β,γ）建立了包含关系。 相似文献
5.
目的：调查和了解张家口地区40岁以上人群2-型糖尿病（T2DM）的患病情况。方法：采用抽样分层横断面现场调查方法对张家口地区40岁以上人群进行问卷调查,并进行口服75g葡萄糖耐量实验。结果：张家口地区40岁以上人群T2DM的患病率为5.86%,糖耐量减低（IGT）患病率为10.43%,男女无明显差异,城市乡村无明显差异。结论：张家口地区40岁以上人群2-型糖尿病（T2DM）的患病率较高。 相似文献
6.
糖化血红蛋白和尿微量蛋白检测的临床意义 总被引：1，自引：0，他引：1
目的：探讨1型糖尿病患者糖化血红蛋白（HbA1c）以及随机尿中尿微量蛋白（β2-MG、mAlb）的检测对发现1型糖尿病早期肾脏损害的临床价值。方法：糖化血红蛋白（HbAlc）用胶乳凝集反应法，尿液微量蛋白（β2-MG、mAlb）用放免法进行测定。结果：HbAlc和尿β2-MG、mAlb在尿蛋白阴性组、尿蛋白阳性组和正常对照组之间差异有显著性（P〈0．01）。结论：尿β2-MG、mAlb和HbAlc的检测可作为诊断1型糖尿病早期肾损害敏感而又可靠的常规指标。 相似文献
7.
ApoE基因多态性和运动与血脂异常的关系 总被引：1，自引：0，他引：1
载脂蛋白E有ApoE2、ApoE3和ApoE4三种主要的异构体,有ε2、ε3和ε4三种常见的等位基因,其基因编码可形成ApoE2/2、ApoE3/3、ApoE4／4三种纯合子表型和ApoE3／2、ApoE4/3、ApoE2／4三种杂合子表型．不同种族间ApoE等位基因的频率和基因型频率都不同．载脂蛋白E基因多态性是个体间血脂水平差异的常见遗传因素,与个体间血脂代谢和血脂异常的发生存在有一定的关联．此外,载脂蛋白E基因多态性可调节运动对血脂的影响作用,但其机制还有待于进一步的研究． 相似文献
8.
Shao-jun ZHU Kui-rong WANG Xiong-xin ZHANG Sheng-mei ZHU 《Journal of Zhejiang University. Science. B》2019,(7):598-604
目的:通过对右美托咪定静脉输注的临床研究,评价中国汉族人群中肾上腺素α2A受体基因多态性与右美托咪定心血管效应的相关性.创新点:探索右美托咪定的心血管系统反应与肾上腺素α2A受体基因多态性的关系,期望为临床个体化应用右美托咪定及其他α2受体激动剂提供重要的依据.方法:本研究取得单位伦理委员会的批准,所有受试者告知受试内容并签署知情同意书.60例美国麻醉医师协会Ⅰ-Ⅱ行择期手术汉族患者根据标准入选本研究.通过静脉输注右美托咪定,记录收缩压、舒张压、心率等心血管效应数据,并用聚合酶链式反应(PCR)法检测ADRA2A基因单核苷酸多态性.结论:中国汉族人群中肾上腺素α2A受体C-1291G基因多态性与应用右美托咪定后心率变化有相关性. 相似文献
9.
目的：观察诺和锐30特充对2型糖尿病患者血浆C-反应蛋白的影响。方法：选择80例2型糖尿病病人,随机分为诺和锐治疗组及诺和灵30R治疗组,诺和锐为餐前即刻皮下注射,诺和灵30R为餐前30min皮下注射,观察两组病人治疗前后餐前、餐后血糖及血浆C-反应蛋白（C-reactive protein,CRP）情况。结果：诺和锐治疗组餐后血糖水平低于诺和灵30R组（P〈0.01）,诺和锐治疗组治疗后C反应蛋白水平明显低于诺和灵30R治疗组（P〈0.01）。结论：对于2型糖尿病患者,诺和锐疗效优于诺和灵30R,而且可以显著降低血浆CRP的水平。 相似文献
10.
《佳木斯教育学院学报》2017,(2)
目的了解汉族人群APOA5基因rs147142271位点遗传多态性与高甘油三酯血症的关系。方法采用SNa Pshot技术分析85例HTG患者和110例正常对照rs147142271位点遗传多态性。结果 HTG患者和正常对照rs147142271位点多态以基因型GG为主,两组内的甘油三酯均值不存在差异,GG基因型为2.125±1.42mmol/L,TG基因型为3.17±0.000mmol/L,TT基因型为2.27±0.000mmol/L,最高为TG基因型,两组间不同基因型个体的甘油三酯水平在男女性别差异上无统计学意义(P=0.677),不同性别不同基因型个体间的甘油三酯水平差异无统计学意义(P=0.181)。结论 rs147142271位点各基因型可能与汉族人群高甘油三脂血症不相关,且在男女性别差异上无统计学意义。 相似文献
11.
INTRODUCTION Recently discovered as an endocrine organ, adi-pose tissue can secrete many cytokines such as Re-sistin, adiponectin and free fatty acids, and receives much attention in the study of insulin resistance and type 2 diabetes mellitus (T2DM). As a peptide hor-mone secreted by adipocyte, Resistin is considered to be the linkage between obesity and insulin resistance (Steppan et al., 2001). Most studies on Resistin in-vestigate adipose tissue. In this experiment we de-tected t… 相似文献
12.
In the search for a rapid and reliable method for identification of bacteria in blood and cerebrospinal fluid , we developed a unified set of primers and used them under polymerase chain reaction(PCR) to amplify the spacer regions between the 16s and 23s genes in the prokaryotic rRNA genetic loci . Spacer regions within these loci showed a significant level of length and sequence polymorphism across most of the species lines. A generic pair of priming sequences was selected from highly conserved sequences in the 16s and 23s genes occurring adjacent to these polymorphic regions. This single set of primers and reaction conditions were used for the amplification of the 16s-23s spacer regions for 61 strains of standard bacteria and corresponding clinical isolates belonging to 20 genera and 27 species, including Listeria, Staphylococcus and Salmonella species, et al. When the spacer amplification products were resolved by electrophoresis, the resulting patterns could be used to distinguish most of the bacteria species within the test group, and the amplification products of the clinical isolates clustered at the standard species level. Some species presenting similar pattern were further analyzed by HinfI or AluI digestion or DNA clone and sequences analysis in order to establish the specific 16s-23s rRNA gene spacer regions map. Analysis of 42 blood specimens from septicemic neonates and 6 CSF specimens from suspected purulent meningitis patients by bacterial culture and PCR-RFLP(Restriction Fregament Length Polymorphism) showed that 15 specimens of blood culture were positive(35.7%) in the 42 septicemic neonates; 27 specimens were positive(64.2%) by PCR, and that the positive rate by PCR was significantly higher than that by blood culture(P<0.01). Among the 6 CSF specimens, one specimen found positive by blood culture was also positive by PCR, two found negative by blood culture showed positive by PCR; all three were S.epidermidis according to the DNA map. One C.neoformans found positive by blood culture showed negative by PCR. The remaining two specimens were both negative by PCR and blood culture. These results indicated that the method of detecting bacterial 16s-23s rRNA spacer regions using PCR and RFLP techniques was rapid, sensitive and specific in the detection of bacterial infections; and so, has very important application in the clinical diagnosis of sepsis in neonates. 相似文献
13.
Objective： Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods： Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline （group B）, vitamin C （group C） and vitamin E （group D）. Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline （sample B）, vitamin C （sample C） and vitamin E （sample D）. Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results： In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion： Vitamin C can protect vascular endothelial cells from mannitol-induced injury. 相似文献
14.
目的:分析检验科血液标本出现误差的原因及探讨应对措施。方法:从我院2018年1月-2020年2月所进行过血液生化检验的血样中随机选取165例血液标本作为研究对象。将检验结果进行回顾性分析,研究探讨检验科血液标本出现误差的原因及应对措施。结果:165例血液标本中有23例标本不合格,不合格率为13.9%,导致血液标本不合格的最主要因素是采集操作不当,总共11例,在不合格血液标本中的占比为47.8%,其次为抗凝剂与血液量比例不当,例数为5例,在不合格血液标本中的占比为21.7%,数据差异具有统计学意义(P<0.05)。结论:检验科血液标本出现误差的原因主要有采集操作不当、保存时间过长、抗凝剂与血液量比例不当等,而血液检验在临床具有重要意义。因此,应加强注意影响检验结果准确性的相关因素,提高检验正确率,使患者能够及时得到正确的治疗方式。 相似文献
15.
目的:了解ABO血型频率和基因频率在不同人群中的分布以及血型与疾病的关系.方法:运用玻片法测定血型,统计并计算满、汉、回三民族ABO血型的基因型频率和基因频率.结果:满、汉、回三民族ABO血型的基因型频率和基因频率存在差异.结论:ABO血型抗原在不同地域不同民族中是有差异的. 相似文献
16.
Early studies examining parenting in the setting of intimate partner violence (IPV) often focus on abuse by the IPV perpetrator or effects of long term exposure. This review addresses how intimate partner violence impacts victim parenting. Seven databases were searched for the time period 1970–2015. Included were comparative studies involving children 11 years or younger. Quality ranking was based on: confirmation of victim status, consideration of co-perpetration, heterogeneity of the population, and standardization of measurements. Of 13,038 studies reviewed, 33 included studies showed that victimization is associated with negative parenting practices. Based on data presented within individual studies, 21 studies were eligible for meta-analysis which demonstrated modest effect sizes with high levels of heterogeneity. There was a negative correlation between IPV and positive parenting (r = -0.08; 95% CI: −.12, − .04); positive correlation between IPV and physical aggression (r = .17; 95% CI: .11, .23) and neglect (r = .12; 95% CI: .01, .23); and a trend toward positive correlation between IPV and psychological aggression (r = .23; 95% CI: −.94, .47). A synthesis of studies unsuitable for meta-analysis reinforced these findings. The review demonstrated ongoing methodological issues with extant literature. 相似文献
17.
Jie DING Hui XU Xiang YIN Fu-rong ZHANG Xiao-ping PAN Yi-an GU Jun-zhu CHEN Xiao-gang GUO 《Journal of Zhejiang University. Science. B》2014,15(3):243-255
The association between the estrogen receptor α gene (ESR1) PvuII polymorphism (c.454-397T>C) and coronary artery disease (CAD) is controversial. Thus, we conducted a meta-analysis to evaluate the relationship. Data were collected from 21 studies encompassing 9926 CAD patients and 16 710 controls. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the relationship between PvuII polymorphism and CAD. The polymorphism in control populations in all studies followed Hardy-Weinberg equilibrium. We found a significant association between ESR1 PvuII polymorphism and CAD risk in all subjects. When the data were stratified by region, a significant association between ESR1 PvuII polymorphism and CAD risk was observed in Asian populations but not in Western populations. The current study suggests that ESR1 PvuII polymorphism has an important role in CAD susceptibility. 相似文献
18.
目的：应用数目可变串联重复序列（VNTR）分子分型技术,对大理地区60株肺结核临床分离株进行分型研究,探讨大理地区菌株DNA多态性及基因型特征。方法：采用VNTR分子分型技术对60株结核分枝杆菌3个VNTR位点进行检测,应用Quantity one软件和BioNumerics 6.6软件进行聚类分析。结果：60株结核分枝杆菌可以分为4个基因群（Ⅰ群、Ⅱ群、Ⅲ群、Ⅳ群）,28个基因型。Ⅰ群占15.0%,含有5个基因型,Ⅱ群占31.7%,含有8个基因型,Ⅲ群占40.0%,含有11个基因型,Ⅳ群占13.3%,含有4个基因型。结论：大理地区的结核分枝杆菌存在基因多态性,其主要流行群为Ⅲ群。 相似文献
19.
Objective: To explore the relationship between genetic polymorphisms in methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR), the central enzymes in folate metabolism that affects DNA methylation and synthesis, and the risk of Down syndrome in China. Methods: Genomic DNA was isolated from the peripheral lymphocytes of 64 mothers of children with Down syndrome and 70 age matched control subjects. Polymerase chain reaction and restriction fragment length polymorphism were used to examine the polymorphisms of MTHFR 677C→T, MTRR 66A→G and the relationship between these genotypes and the risk of Down syndrome was analyzed. Results: The results show that the MTHFR 677C→T polymorphism is more prevalent among mothers of children with Down syndrome than among control mothers, with an odds ratio of 3.78 (95% confidence interval (CI), 1.78～8.47). In addition, the homozygous MTRR 66A→G polymorphism was independently associated with a 5.2-fold increase in estimated risk (95% CI, 1.90～14.22). The combined presence of both polymorphisms was associated with a greater risk of Down syndrome than the presence of either alone, with an odds ratio of 6.0 (95% CI, 2.058～17.496).The two polymorphisms appear to act without a multiplicative interaction. Conclusion: MTHFR and MTRR gene mutation alleles are related to Down syndrome, and CT, TT and GG gene mutation types increase the risk of Down syndrome. 相似文献