共查询到20条相似文献，搜索用时 31 毫秒
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目的：观察TGF-β1作用下,肺泡Ⅱ型上皮细胞RLE-6TN在EMT过程中HDAC1、8的表达情况及TSA对TGF-β1诱导细胞EMT的影响。方法：将TGF-β1加入到体外培养的细胞中,于不同时间收取细胞,采用WB及Real-time RT-PCR检测HDAC1、8及E-cad、α-SMA的表达情况。然后将TGF-β1加入经过TSA预处理的细胞中,于不同时间收取细胞,重新检测上述基因。结果：加入TGF-β1后,E-cad蛋白表达下调;α-SMA蛋白表达上调;HDAC1蛋白表达上调;HDAC8蛋白表达下调。E-cad mRNA表达下调;α-SMA及HDAC8的mRNA均于12 h表达上调,6 h、24 h表达下调;HDAC1 mRNA表达于6 h下调,24 h上调。加入TSA后,E-cad蛋白表达上调;α-SMA、HDAC1及HDAC8蛋白的表达均下调。E-cad mRNA表达上调;α-SMA mRNA表达于6 h、12 h上调,24 h下调;HDAC1 mRNA表达下调;HDAC8 mRNA表达上调。结论：TGF-β1体外诱导的RLE-6TN细胞EMT,可以通过应用TSA抑制其获得α-SMA表型、失去E-cad表型,从而部分逆转TGF-β1诱导的肺泡Ⅱ型上皮EMT。 相似文献
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[目的]观察双环醇对慢性乙型病毒性肝炎患者肝纤维化程度以及肝组织内转化生长因子β1(TGF-β1)和Ⅰ、Ⅲ型胶原含量的影响.[方法]20例慢性乙型病毒性肝炎患者以双环醇75~150 mg/d治疗24周,治疗前后分别肝活检进行常规病理学检查,并应用免疫组化技术和多媒体彩色病理图文分析系统观察双环醇治疗前后肝组织内TGF-β1和Ⅰ、Ⅲ型胶原含量的变化.[结果]慢性乙型病毒性肝炎肝纤维化患者经双环醇治疗后,肝组织炎症和纤维化程度明显改善,肝组织内TGF-β1和Ⅰ、Ⅲ型胶原含量均较治疗前明显降低,差异有显著性(P<0.05).[结论]双环醇治疗可以减轻慢性乙型病毒性肝炎患者肝脏炎症反应,同时明显降低慢性乙型病毒性肝炎患者肝组织内TGF-β1和Ⅰ、Ⅲ型胶原的含量,从而发挥抗纤维化作用. 相似文献
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Chuan ZUO Xi-sheng XIE Hong-yu QIU Yao DENG Da ZHU Jun-ming FAN 《Journal of Zhejiang University. Science. B》2009,10(5)
Astragalus mongholicus (AM) derived from the dry root of Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is a widely used traditional Chinese medicine. The present study investigated the potential role of AM on renal fibrosis on a rat model of unilateral ureteral obstruction (UUO). We divided 48 Sprague-Dawley rats randomly into 4 groups: sham-operated group (Sham), untreated UUO group, AM-treated (10 g/(kg.d)) UUO group, and losartan-treated (20 mg/(kg.d)) UUO group as positive control. Haematoxylin & eosin (HE) and Masson staining were used to study the dynamic histological changes of the kidneys 7 and 14 d after operation. The expressions of fibronectin (FN), type I collagen (coII), hepatocyte growth factor (HGF), transforming growth factor-pi (TGF-βl), and a-smooth muscle actin (a-SMA) were analyzed by real-time polymerase chain reaction (PCR), immunohistochemistry staining, and Western blot. Results show that, similar to losartan, AM alleviated the renal damage and decreased the deposition of FN and colI from UUO by reducing the expressions of TGF-pi and a-SMA (P<0.05), whereas HGF increased greatly with AM treatment (P<0.05). Our findings reveal that AM could retard the progression of renal fibrosis. The renoprotective effect of AM might be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-β1 expression. 相似文献
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目的：探讨转化生长因子β1（TGF-β1）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）及肾母细胞瘤抑癌基因蛋白（WT1）在肾活检肾小球肾炎组织中的表达及其意义。方法：采用Envision法对40例肾小球肾炎组织及7例对照组肾组织进行TGF-β1、Caspase-3和WT1免疫组织化学染色标记、分析。结果：TGF-β1、Caspase-3、WT1在各种肾炎组织中均有表达,各实验组与对照组差异有统计学意义（P〈0.05）。TGF-β1在系膜增生性肾小球肾炎组的表达明显低于膜性肾病组（t=-4.131,P=0.002）;Caspase-3在各组别中其表达差异无统计学意义;WT1在大量高蛋白尿组表达低于非大量蛋白尿组（t=-2.130,P=0.046）;其余组别差异无统计学意义。WT1的低表达与TGF-β1、Caspase-3的高表达相关。结论：TGF-β1、Caspase-3表达的上调及WT1表达的下调与肾病理改变密切相关,蛋白尿和足细胞减少可能是肾脏病进展的预测因子及肾小球硬化的原因。 相似文献
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Zhan-huai WANG ;Qiong LI ;Shu-qin RUAN ;Qian XIAO ;Yue LIU ;Ye-ting HU ;Li-feng HU ;Hai-yan CHEN ;Shu ZHENG ;Su-zhan ZHANG ;Ke-feng DING 《Journal of Zhejiang University. Science. B》2014,15(8):701-712
研究目的:探索小分子酪氨酸激酶抑制剂舒尼替尼(sunitinib)对结肠肿瘤微环境中的肿瘤相关成纤维细胞的作用及其机制。创新要点:舒尼替尼通过抑制肿瘤间质成纤维细胞的生长,间接发挥抗肿瘤效应,为结肠癌综合治疗的提供新途径。研究方法:通过细胞周期分析和细胞增殖测定进行舒尼替尼体外抑制肿瘤细胞的研究。采用Western-blot检测磷酸化血小板衍生生长因子β受体(PDGFR-β)、蛋白激酶B(Akt)及细胞外信号调节蛋白激酶(ERK)的蛋白水平。通过注射肠腺癌细胞株SW620和结肠成纤维细胞构建的裸鼠移植瘤模型来研究舒尼替尼的体内抑瘤效果。重要结论:舒尼替尼可有效抑制结肠癌来源的原代结肠成纤维细胞生长,该抑制作用主要通过抑制血小板衍生生长因子(PDGF)信号通路得以实现。 相似文献
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Hai-tao REN Hang HU Yuan LI Hong-fei JIANG Xin-lei HU Chun-mao HAN 《Journal of Zhejiang University. Science. B》2013,14(3):224-230
Objective:The present study was designed to use an in vivo rabbit ear scar model to investigate the efficacy of systemic administration of endostatin in inhibiting scar formation.Methods:Eight male New Zealand white rabbits were randomly assigned to two groups.Scar model was established by making six full skin defect wounds in each ear.For the intervention group,intraperitoneal injection of endostatin was performed each day after the wound healed(about 15 d post wounding).For the control group,equal volume of saline was injected.Thickness of scars in each group was measured by sliding caliper and the scar microcirculatory perfusion was assessed by laser Doppler flowmetry on Days 15,21,28,and 35 post wounding.Rabbits were euthanatized and their scars were harvested for histological and proteomic analyses on Day 35 post wounding.Results:Macroscopically,scars of the control group were thicker than those of the intervention group.Significant differences between the two groups were observed on Days 21 and 35(p<0.05).Scar thickness,measured by scar elevation index(SEI) at Day 35 post wounding,was significantly reduced in the intervention group(1.09±0.19) compared with the controls(1.36±0.28).Microvessel density(MVD) observed in the intervention group(1.73±0.94) was significantly lower than that of the control group(5.63±1.78) on Day 35.The distribution of collagen fibers in scars treated with endostatin was relatively regular,while collagen fibers in untreated controls were thicker and showed disordered alignment.Western blot analysis showed that the expressions of type I collagen and Bcl-2 were depressed by injection of endostatin.Conclusions:Our results from the rabbit ear hypertrophic scar model indicate that systemic application of endostatin could inhibit local hypertrophic scar formation,possibly through reducing scar vascularization and angiogenesis.Our results indicated that endostatin may promote the apoptosis of endothelial cells and block their release of platelet-derived growth factor(PDGF) and fibroblast growth factor(FGF),thereby controlling collagen production by fibroblasts.Blood vessel-targeted treatment may be a promising strategy for scar therapy. 相似文献
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Shu-fan ZHAO Mao-zhou CHAI Min WU Yong-hong HE Tian MENG Bing SHI 《Journal of Zhejiang University. Science. B》2014,15(3):289-294
The purpose of this study was to investigate the effect of vitamin B12 on palatal development by co-administration of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and dexamethasone (DEX). We examined the morphological and histological features of the palatal shelf and expression levels of key signaling molecules (transforming growth factor-β3 (TGF-β3) and TGF-β type I receptor (activin receptor-like kinase 5, ALK5)) during palatogenesis among a control group (Group A), TCDD+DEX exposed group (Group B), and TCDD+DEX+vitamin B12 exposed group (Group C). While we failed to find that vitamin B12 decreased the incidence of cleft palate induced by TCDD+DEX treatment, the expression levels of key signaling molecules (TGF-β3 and ALK5) during palatogenesis were significantly modulated. In TCDD+DEX exposed and TCDD+DEX+vitamin B12 exposed groups, palatal shelves could not contact in the midline due to their small sizes. Our results suggest that vitamin B12 may inhibit the expression of some cleft palate inducers such as TGF-β3 and ALK5 in DEX+TCDD exposed mice, which may be beneficial against palatogenesis to some degree, even though we were unable to observe a protective role of vitamin B12 in morphological and histological alterations of palatal shelves induced by DEX and TCDD. 相似文献
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张文慧 《扬州职业大学学报》2023,(1):11-15
伊瑟尔的文本空白理论强调作者与读者以文本为媒介的交流互动,揭示了文本意义由表象性“残缺”趋于实质性“丰满”的过程。究其原因,一方面,“残缺”对应作者创作,由文本的召唤性及作者的读者分身意识造成; 另一方面,“丰满”针对读者接受,与其在填补空白时所经历的二次否定有关,而位于文本虚构两端的现实与想象恰是勾连“残缺”与“丰满”的桥梁。张爱玲的小说以其富有的“空白”结构生动地体现了以上变化过程及其内部规律。 相似文献
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Chuan Zuo Xi-sheng Xie Hong-yu Qiu Yao Deng Da Zhu Jun-ming Fan 《Journal of Zhejiang University. Science. B》2009,10(5):380-390
Astragalus mongholicus (AM) derived from the dry root of Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is a widely used traditional Chinese medicine. The present study investigated the potential
role of AM on renal fibrosis on a rat model of unilateral ureteral obstruction (UUO). We divided 48 Sprague-Dawley rats randomly
into 4 groups: sham-operated group (Sham), untreated UUO group, AM-treated (10 g/(kg·d)) UUO group, and losartan-treated (20
mg/(kg·d)) UUO group as positive control. Haematoxylin & eosin (HE) and Masson staining were used to study the dynamic histological
changes of the kidneys 7 and 14 d after operation. The expressions of fibronectin (FN), type I collagen (colI), hepatocyte
growth factor (HGF), transforming growth factor-β1 (TGF-β1), and α-smooth muscle actin (α-SMA) were analyzed by real-time
polymerase chain reaction (PCR), immunohistochemistry staining, and Western blot. Results show that, similar to losartan,
AM alleviated the renal damage and decreased the deposition of FN and colI from UUO by reducing the expressions of TGF-β1
and α-SMA (P<0.05), whereas HGF increased greatly with AM treatment (P<0.05). Our findings reveal that AM could retard the progression of renal fibrosis. The renoprotective effect of AM might
be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-β1 expression.
Project (No. 30170437) supported by the National Natural Science Foundation of China 相似文献
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[目的]探讨拉米夫定治疗早、中期慢性重型肝炎的疗效,观察血清TNF-α、TGF-β1的变化.[方法]121例早、中期慢性重型肝炎患者分两组,均以综合治疗为主,治疗组加用拉米夫定100 mg,每日1次,共2个月.比较两组患者治疗前后肝功能,HBeAg、HBV-DNA、TGF-β1、TNF-α的变化,及病死率的差异.[结果]治疗组患者血清HBeAg,HBV-DNA转阴明显高于对照组,血清总胆红素,TNF-α、TGF-β1较对照组下降明显,病死率也下降.[结论]慢性重型肝炎早、中期应该尽早应用抗病毒药物,这对减少慢性重型肝炎的病死率有益. 相似文献
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丁喜霞 《洛阳师范学院学报》2006,25(3):104-107
“疾”、“病”和“疾病”的各种意义在先秦文献中都已出现,没有时间上的明显先后。“疾”和“病”在表示“疾病”义时也无意义上的差别,同义并列不构成句法上的并列关系,即“疾病”不能构成并列短语。“病”由“困苦”引申为引发困苦之一的“疾病”义,二者的意义联系也很紧密。因此,名词“疾病”是通过词法途径并列两个同义单音词形成的,与同形的主谓短语不具有衍生关系。 相似文献
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设C是一致光滑Banach空间X的一个闭凸子集,T：C→C是非扩张映象且不动点集F（T）≠Φ,f：C→C是一个固定的压缩映射.序列{xn}由下式定义：xn＋1=αnf（xn）＋（1-αn）（βnxn＋（1-βn）Txn）其中αn,βn∈（0,1）.当αn和βn满足一定条件时,则序列{xn}强收敛到T的不动点. 相似文献
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以牛肌腱为原料,分别利用酸-酶结合法和酶法从冻干碎块中提取胶原纤维,经过盐析、透析、离心和冷冻干燥后制备得到高纯度胶原纤维.通过测定胶原纤维中羟脯氨酸的含量,得到酸-酶结合法的得率为44.9%,远大于单独用酶提取胶原纤维的得率33.8%.对制备的胶原纤维进行红外扫描和差示量热扫描(DSC),结果表明胶原纤维保持了完整的三股螺旋结构,DSC扫描结果表明冻干胶原纤维的变性温度为60.8℃. 相似文献
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Although many U.S. children can count sets by 4 years, it is not until 5½–6 years that they understand how counting relates to number—that is, that adding 1 to a set necessitates counting up one number. This study examined two knowledge sources that 3½- to 6-year-olds (N = 136) may leverage to acquire this “successor function”: (a) mastery of productive rules governing count list generation; and (b) training with “+1” math facts. Both productive counting and “+1” math facts were related to understanding that adding 1 to sets entails counting up one number in the count list; however, even children with robust successor knowledge struggled with its arithmetic expression, suggesting they do not generalize the successor function from “+1” math facts. 相似文献
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古建筑模型制作技艺是中国传统造物实践和审美理念的重要表现,也是国家级非物质文化遗产之一。中国传统模型制作技艺在唐宋时期已有高水平发展,清代“样式雷”的“烫样”模型更是达到“空前之作”的技艺高度。从“精益求精”的匠技实践、“传统礼制”的象征表达、“以人为本”的美学理想三个文化层面对技艺进行解读,提出符合新时代古建筑模型制作技艺“适应性创造”的“再传承”理念。探索模型制作技艺在当代传承与发展中的更多可能性,在审美范式上对其加以提升,从而树立新时代造物思想和美的“范式”。 相似文献
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“非吏比者三老、北边骑士,轺车一算;商贾人轺车二算”一句中的“非”字对“吏比者三老”、“北边骑士”构成否定关系,“非吏比者”不能合为一个词,“吏比者”专指三老。 相似文献
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贺春英 《黑龙江教育学院学报》2005,24(5):77-78
中西文化的相异之处主要体现在四个方面:东方内容上的“和谐”与西方形式上的“和谐”;西方的再现、模仿、写实与东方的表现、抒情、言志;西方的“典型论”与中国的“意境说”;西方的“美与真”的统一与中国的“美与善”的结合。 相似文献