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云南石林石灰山地恶劣的自然条件造就了当地独特的观赏植物资源，这里的观赏植物属于石灰岩特有种或偏好于石灰岩地区生长的种类，抗性强、根深、叶小、具有典型的耐旱特征，能在土壤脊薄的裸露岩石缝隙中生长．选育适应当地环境的乡土野生观赏植物资源进行开发利用，对促进石林生态美县建设具有重要意义。 相似文献
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Transient expression of chicken alpha interferon gene in lettuce 总被引：1，自引：0，他引：1
We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry. 相似文献
4.
从人类生态学的视角看哈尼族的采集与狩猎 总被引：1，自引：0，他引：1
云南哀牢山区的哈尼族的经济类型自古以来以梯田农耕生产为主,成为哈尼族代表性的文化景观。但哀牢山区的动植物资源又为哈尼族的采集和狩猎提供了丰富的自然资源,成为哈尼族不可抹杀的物质生产活动方式。从人类生态学的视角考察哈尼族采集和狩猎生产方式,可以透视采集与狩猎生态文化内涵。 相似文献
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Ling-zhe Huang Li-xia Rao Xue-ping Zhou Jian-xiang Wu 《Journal of Zhejiang University. Science. B》2013,14(10):875-885
Rice stripe virus (RSV) is the type member of the genus Tenuivirus. RSV is known to have four segmented, single-stranded RNA molecules and causes rice stripe disease in the rice fields of China, Japan, and Korea. Based on the complete genomic sequences of the determined 6 RSV isolates (from Yunnan, Jiangsu, Zhejiang, and Liaoning Provinces, China) and 27 other RSV isolates (from Yunnan, Jiangsu, Anhui, Henan, and Shandong Provinces of China, also Japan and Korea) downloaded from GenBank, we provided a genotyping profile of RSV field isolates and described the population structure of RSV. All RSV isolates, except isolate CX, could be divided into two subtypes, one including 6 isolates from Yunnan Province, and the other including 26 isolates from different parts of China, Japan, and Korea, which were referred to as subtype II and subtype I, respectively. The amino acid distances between subtypes range from 0.053 to 0.085. RSV isolates in Yunnan Province were genetically differentiated from other parts of China, Japan, and Korea and showed infrequent gene flow. The RSV populations collected from other parts of China, Japan, and Korea were only composed of subtype I and showed very low genetic diversity. We speculated that isolate CX may be the result of recombination of isolates from two subtypes. Two potential recombination events were detected in RNA4 of isolate CX. 相似文献
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Liu Y Zhang CZ Yu XY Zhang ZY Zhang X Liu RR Liu XJ Gong ZM 《Journal of Zhejiang University. Science. B》2007,8(12):900-905
A high affinity polyclonal antibody-based enzyme linked immunosorbent assay （ELISA） was developed for the quantification of zeranol in bovine urine. On the basis of urine matrix studies, the optimized dilution factors producing insignificant matrix interference were selected as 1：5 in pretreatment. In the improved ELISA, the linear response range was between 0.02 and 1 μg/ml, and the detection limit was 0.02 μg/ml for the assay. The overall recoveries and the coefficients of variation （CVs） were in the range of 82%-127% and 3.5%-8.8%, respectively. Thirty-six bovine urine samples spiked with zeranol （ranging from 0.2 to 10 μg/ml） were detected by the ELISA and liquid chromatography （LC） method, and good correlations were obtained between the two methods （R^2=0.9643）. We conclude that this improved ELISA is suitable tool for a mass zeranol screening and can be an altemative for the conventional LC method for zeranol in bovine urine. 相似文献
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Objective: to explore a new serological method for detectingHelicobacter pylori (H. pylori) infection. Methods: Serum soluble antigen ofH. pylori was detected by using avidin-biotin ELISA technique to evaluate the status ofH. pylori infection and for comparison with rapid urease test (RUT), histologic examination and serology. Results: The sensitivity,
specificity, positive predictive value and negative predictive value were 77.46%, 91.07%, 91.67% and 76.12%, respectively.
The prevalence rate of serumH. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by14C-UBT methods (P>0.05). Conclusions: The detection of serumH. pylori soluble antigen(HpSAg) could be used as a new serological method which is accurate, and convenient, not affected by the memorizing
reaction of serum antibody; is more sensitive, more specific and suitable for clinical diagnosis, and evaluation of eradication
and for follow-up ofH. pylori as well as for detection in children and pregnant women.
Project supported by Zhejiang Provincial Health Bureau (No. 2000A118), China 相似文献
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伴随着中国—东盟自由贸易区的构建,中国与东盟的各种合作不断发展和加强,其中旅游业是拉动中国—东盟经济发展的重要引擎。作为中国面对东盟的桥头堡城市和两个扇面的节点地区———云南省,面临此项历史性合作,在迎来了发展本省旅游业的机遇时,也面临着来自各方面的挑战。本文在简单阐述了中国—东盟自由贸易区的构建对中国旅游业发展的意义后,重点分析了它对云南省旅游业的各方面的影响,强调了在具有前所未有的旅游发展机遇的同时,也面临巨大的挑战。 相似文献
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经过标本采集、分类签定和调查,筛选出17科33种具有一定观赏价值的云南省大关县野生草本植物,对其观赏特性进行研究,为云南野生花卉的开发利用和保护提供基础的科学依据,为开发云南具有知识产权的花卉品种奠定基础. 相似文献
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利用2002~2013年统计资料,以云南为例进行相关研究,研究表明:1.城镇化和经济发展间存在复杂多变的耦合关系;2.政府政策对于地区经济社会发展具有重要的引导作用;3.其协调度空间分布差异显著。对此,发展较好的地区中心城市,应注重培育区域经济增长极;城镇化水平限制经济发展的地区,应消除限制因素,发展特色小城镇;经济发展与城镇化水平均较低的地区,应发展特色产业和特色小城镇,以此推动城镇化进程。 相似文献
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Transgenic Brassica compestris L.spp.chinensis plants expressing a choline oxidase(codA) gene from Arthrobacter globiformis were obtained through Agrobacterium tumefaciens-mediated transformation.In the transgenic plants,codA gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay(ELISA) examination,immunogold localization,and 1 H-nuclear magnetic resonance( 1 H-NMR) . Stress tolerance was evaluated in the T3 plants under extreme temperature and salinity conditions.The plants of transgenic line 1(L1) showed significantly higher net photosynthetic rate(Pn) and Pn recovery rate under high(45°C,4 h) and low temperature(1°C,48 h) treatments,and higher photosynthetic rate under high salinity conditions(100,200,and 300 mmol/L NaCl,respectively) than the wild-type plants.The enhanced tolerance to high temperature and high salinity stresses in transgenic plants is associated with the accumulation of betaine,which is not found in the wild-type plants.Our results indicate that the introduction of codA gene from Arthrobacter globiformis into Brassica compestris L.spp.chinensis could be a potential strategy for improving the plant tolerance to multiple stresses. 相似文献
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云南省高校学报办刊现状与发展 总被引：1，自引：0，他引：1
张云平 《云南师范大学学报(哲学社会科学版)》2006,38(4):136-140
选取云南省16家有代表性的高校学报（本科高校13家，地方性院校2家，专科学校1家）和省外6家高校学报，从8个观测点51个子项目的调查中，着重选取了：学报出版基本情况；建制及人员情况；稿件状况（刊用率和内外稿比例等）；办刊经费和物质条件；学报的社会影响及认可度；学报编辑人员在学校中的地位等方面内容。运用详尽的第一手材料和数据，比较分析了云南省高校学报之间以及省内外高校学报办刊中的差异，从宏观上提出云南省打造品牌学报的发展思考。 相似文献
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云南地处西南边疆,限于地理环境因素的制约,其社会发展进程远比内地缓慢得多,元“大一统”后,历代中央王朝统治者认识到了云南在全国的国防、经济地位,从而加强了云南的边疆建设.明末陈用宾主政云南巡抚时,审时度势,因地制宜,不仅对云南的边疆国防极为重视,而且大力发展边疆经济,构建边疆文化,为云南以后的发展奠定了基础. 相似文献
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We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry. 相似文献
15.
Rui Chen Shao-hong Lu Qun-bo Tong Di Lou Dong-yan Shi Bing-bing Jia Guo-ping Huang Jin-fu Wang 《Journal of Zhejiang University. Science. B》2009,10(7):512-521
The dense granule protein 4 (GRA4) is a granular protein from Toxoplasma gondii, and is a candidate for vaccination against this parasite. In this study, the plasmid pcDNA3.1-GRA4 (pGRA4), encoding for
the GRA4 antigen, was incorporated by the dehydration-rehydration method into liposomes composed of 16 mmol/L egg phosphatidylcholine
(PC), 8 mmol/L dioleoyl phosphatidylethanolamine (DOPE), and 4 mmol/L 1,2-diodeoyl-3-(trimethylammonium) propane (DOTAP).
C57BL/6 mice and BALB/c mice were immunized intramuscularly three times with liposome-encapsulated pGRA4 to determine whether
DNA immunization could elicit a protective immune response to T. gondii. Enzyme-linked immunosorbent assay (ELISA) of sera from immunized mice showed that liposome-encapsulated pGRA4 generated
high levels of IgG antibodies to GRA4. Production of primary interferon (IFN)-γ and interleukin (IL)-2 in GRA4-stimulated
splenocytes from vaccinated mice suggested a modulated Th1-type response. 72.7% of C57BL/6 mice immunized with liposome-encapsulated
pGRA4 survived the challenge with 80 tissue cysts of ME49 strain, whereas C57BL/6 mice immunized with pGRA4 had only a survival
rate of 54.5%. When immunized BALB/c mice were intraperitoneally challenged with 103 tachyzoites of the highly virulent RH strain, the survival time of mice immunized with liposome-encapsulated pGRA4 was markedly
longer than that of other groups. Our observations show that liposome-encapsulated pGRA4 enhanced the protective effect against
infection of T. gondii.
Project supported by the Science Foundation of the Health Bureau of Zhejiang Province, China (Nos. 2003QN003 and 2005A001)
and the Science Foundation of the Science and Technology Department of Zhejiang Province, China (No. 2006C13022) 相似文献
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采用分层整群随机抽样方法,使用SES自尊量表对云南省6所大学1240名研究生进行调查,结果发现:(1)研究生低自尊水平、中间水平、高自尊水平的比例分别为12.6%、66.3%、20.7%;(2)不同培养类型研究生的自尊状况有显著差异;(3)个人不同月支出研究生自尊有显著差异;(4)父亲不同教育水平研究生自尊状况差异显著;(5)母亲不同教育水平研究生自尊状况差异显著. 相似文献
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报道了宁夏、云南和四川三个省区的8个丝孢菌新记录种,分别是宁夏回族自治区新记录种荞麦尾孢Cercospora fagopyri、轮纹尾孢C.zonata、仁果枝孢Cladosporium malorum、藜钉孢Passalora dubia、插天泡假尾孢Pseudocercospora rubi、稍厚柱隔孢Ramularia crassiuscula;云南省新记录种紫草柱隔孢R.cerinthes;四川省新记录种莴苣柱隔孢R.lactucosa. 相似文献
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ZHANG Qing-yu WEI Yu-mei CHEN Ying-xu GUO Hui 《浙江大学学报(A卷英文版)》2007,8(11):1816-1825
Electricity consumption increases rapidly with the rapid development of China. The environmental damage costs of electricity generation are very important for both policy analysis and the proper management of the environment. A method was developed in this work to estimate gross environmental damage costs according to emission inventory and environmental cost factors, and to extend the costs from provincial to national level with population density. In this paper, sulfur dioxide (SO2), nitrogen oxides (NOx), particulate matter less than 10 μm in diameter (PM10), and carbon dioxide (CO2) from fossil fired power plants over 6000 kW were selected as index pollutants to quantify the environmental costs of damages on human health and global warming. With the new developed method, environmental damage costs, caused by 3 types of fired power plants in 30 provinces and 6 economic sectors during the years 2000 to 2003, were evaluated and analyzed. It can be seen that the calculated total national environmental damage costs of electricity have rapidly increased from 94930.87×106 USD in 2000 to about 141041.39×106 USD in 2003, with an average annual growth rate of 14.11%. Environmental damage costs of SO2, NOx, PM10, and CO2 are 69475.69×106, 30079.29×106, 28931.84×106, and 12554.57×106 USD and account for 49.26%, 21.33%, 20.51%, and 8.90% of total environmental costs in fossil electricity generation, respectively. With regard to regional distribution, external costs caused by fossil electricity generation are mainly concentrated in the more populated and industrialized areas of China, i.e., the Eastern Central and Southeastern areas. 相似文献
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Objective: to explore a new serological method for detecting Helicobac ter pylori ( H. pylori ) infection. Methods: Serum soluble antigen of H. p ylor i was detected by using avidin-biotin ELISA technique to evaluate the status of H. pylori infection and for comparison with rapid urease test ( RUT ), histo logi c examination and serology. Results: The sensitivity, specificity, positive pred ictive value and negative predictive value were 77.46%, 91.07%, 91.67% a nd 76.12 %, respectively. The prevalence rate of serum H. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by 14 C-UBT met hods ( P>0.05 ). Conclusions: The detection of serum H. pylori solub le antigen( HpSAg) could be used as a new serological method which is accurate, and convenie nt, not affected by the memorizing reaction of serum antibody; is more sensitive , m ore specific and suitable for clinical diagnosis, and evaluation of eradication and for follow-up of H. pylori as well as for detection in children and pre gnant women. 相似文献