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1.
Qin Guo Wei Zhang Liu-Liu Ma Qi-He Chen Ji-Cheng Chen Hong-Bo Zhang Hui Ruan Guo-Qing He 《Journal of Zhejiang University. Science. B》2010,11(1):41-51
The aim of this work was to construct a novel food-grade industrial arming yeast displaying β-1,3-1,4-glucanase and to evaluate the thermal stability of the glucanase for practical application. For this purpose, a bi-directional vector containing galactokinase (GAL1) and phosphoglycerate kinase 1 (PGK1) promoters in different orientations was constructed. The β-1,3-1,4-glucanase gene from Bacillus subtilis was fused to α-agglutinin and expressed under the control of the GAL1 promoter. α-galactosidase induced by the constitutive PGK1 promoter was used as a food-grade selection marker. The feasibility of the α-galactosidase marker was confirmed by the growth of transformants harboring the constructed vector on a medium containing melibiose as a sole carbon source, and by the clear halo around the transformants in Congo-red plates owing to the expression of β-1,3-1,4-glucanase. The analysis of β-1,3-1,4-glucanase activity in cell pellets and in the supernatant of the recombinant yeast strain revealed that β-1,3-1,4-glucanase was successfully displayed on the cell surface of the yeast. The displayed β-1,3-1,4-glucanase activity in the recombinant yeast cells increased immediately after the addition of galactose and reached 45.1 U/ml after 32-h induction. The thermal stability of β-1,3-1,4-glucanase displayed in the recombinant yeast cells was enhanced compared with the free enzyme. These results suggest that the constructed food-grade yeast has the potential to improve the brewing properties of beer. 相似文献
2.
Boys and girls love to watch TV.Some childrenspend(花费)six hours a day in school andfour to six hours a day in front of the TV set.Somechildren even(甚至) watch TV for eight or more hourson Saturday.TV programmes are like books and films.A child canlearn bad things from some of them and good things from theothers.Some programmes (节目)help the children to knowthe news from the other parts of the world.Some programmes showpeople and places from other countries or other times in history.With TV,a child doesn't need to go to the zoo to see animals(动物)or to 相似文献
3.
Needs Analysis An investigation, in the light of aspecification of the tasks a learner or group of learners will berequired to perform in the TL, of what particular aspects of theTL need to be learnt in order to bring about proficiency in theseparticular tasks. The results of the needs analysis can then beused to determine a syllabus and suitable teaching techniques. Norm(ⅰ) The mode of speech and the grammatical us 相似文献
4.
周丽丽 《湖州师范学院学报》2003,25(Z1):159-164
To those whose interest is in translation, an important topic for study is the choice of words. As lexicon is the mostactive and the liveliest component of a language, in the course of translation, due attention must be given to the analysis andcomparison of words and phrases before a suitable word or phrase is chosen to convey the meaning in the original version.Meanwhile, English words and phrases are rich and the same words or phrases may be different in meaning in a certain context,therefore, the diction in translation from English to Chinese is undoubtedly a matter of importance. 相似文献
5.
Pun is one of the most common figures of speech in English. It is the humorous use of a word in such a way as to sug?gest different meanings or applications or of words having the same or nearly the sa... 相似文献
6.
曾辉 《成都教育学院学报》2001,15(5):11-12
In Otto Jespersen‘s book the Philosophy of Grammar( 1951 ),the definition of formulas is mentioned: “a formula may be a whole sentence or a group of words, or it may be one word, or it may be only part of a word, that is not improtant,but it must always be something which to the actual speech- instinct is a unit which cannot be further analyzed or decomposed in the way a free combination can”(1951:24) .Thus proverbs belong to formulas, as the example “Handsome is that handsome does” given by Jespersen. 相似文献
7.
Acute phase reaction and acute phase proteins 总被引：18，自引：0，他引：18
Gruys E Toussaint MJ Niewold TA Koopmans SJ 《Journal of Zhejiang University. Science. B》2005,6(11):1045-1056
INTRODUCTION The first reaction of the body to immunological stress is the innate, non-specific response preceding specific immune reactions. The acute phase response (APR) is a prominent systemic reaction of the or- ganism to local or systemic disturbances in its ho- meostasis caused by infection, tissue injury, trauma or surgery, neoplastic growth or immunological disor- ders (Gordon and Koy, 1985; Gruys et al., 1999). At the site of invasion by a micro-organism and the place of tissu… 相似文献
8.
Qihua PAN Junzhi LUO Yuewen JIANG Zhi WANG Ke LU Tiansheng CHEN 《Journal of Zhejiang University. Science. B》2022,23(1):74-83
Generation of mutants with clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9)is commonly carried out in fish species by co-injecting a mixture of Cas9 messenger RNA(mRNA)or protein and transcribed guide RNA(gRNA).However,the appropriate expression system to produce functional gRNAs in fish embryos and cells is rarely present.In this study,we employed a poly-transfer RNA(tRNA)-gRNA(PTG)system driven by cytomegalovirus(CMV)promoter to target the medaka(Oryzias latipes)endogenous gene tyrosinase(tyr)or paired box 6.1(pax6.1)and illustrated its function in a medaka cell line and embryos.The PTG system was combined with the CRISPR/Cas9 system under high levels of promoter to successfully induce gene editing in medaka.This is a valuable step forward in potential application of the CRISPR/Cas9 system in medaka and other teleosts. 相似文献
9.
张宏 《高等教育与学术研究》2006,(2)
The term, culture shock, was introduced for the first time in 1958 to describe the anxiety produced when a person moves to a completely new environ- ment. This term expresses the lack of direction, the feeling of not knowing what to do or how to do things in a new environment, and not knowing what is appro- priate or inappropriate. The feeling of culture shock generally sets in after the first few weeks of coming to a new place. "Coming to America is like jumping into cold water," says an inte… 相似文献
10.
TBL, also known as TBLT (task-based learning and teaching) and, in the USA, as TBL (task-based instruction) is an approach which uses communication tasks as the central focus for language teaching and learning. Basically the term task in TBL, refers to a meaning -focused activity. There are a number of definitions in the literature, but they can be characterized also follows: the learner s principle focus is on exchanging and understanding meanings, rather than on practice of pre-specified forms or patterns there is some kind of communicative purpose or goal set for the task, so learners know what they are expected to achieve by the end tasks can involve any or all four skills, e. g. a 相似文献
11.
A text to speech interface for Universal Digital Library 总被引：1，自引：0，他引：1
PRAHALLAD Kishore BLACK Alan 《浙江大学学报(A卷英文版)》2005,6(11):1229-1234
INTRODUCTION The objective of Universal Digital Library (UDL) is to capture all books in digital format (Universal Digital Library, 2005; Digital Library of India, 2005). Most of the digital information present in the digital world is accessible to a few who can read or under- stand a particular language. Language technologies can provide solutions in the form of natural interfaces so that digital content can reach the masses and fa- cilitate the exchange of information across differe… 相似文献
12.
A chaotic dynamical system is characterized by a positive averaged exponential separation of two neighboring trajectories over a chaotic attractor. Knowledge of the Largest Lyapunov Exponent λ1 of a dynamical system over a bounded attractor is necessary and sufficient for determining whether it is chaotic (λ1＞0) or not (λ1≤0). We intended in this work to elaborate the connection between Local Lyapunov Exponents and the Largest Lyapunov Exponent where an altemative method to calculate λ1has emerged. Finally, we investigated some characteristics of the fixed points and periodic orbits embedded within a chaotic attractor which led to the conclusion of the existence of chaotic attractors that may not embed in any fixed point or periodic orbit within it. 相似文献
13.
Mark lived in a house at the top of a very steep (陡斜的) street. Every day he ran down the street to go to school or play with his friends. 相似文献
14.
Naveed Shibli 《美中教育评论》2009,6(5):61-66
Higher education is the product of three constituents, the policy makers or government, the executors or faculty and the recipients or the students or scholars. Steadiness among different constituents in given social interaction can act as facilitating motive and it could make higher education more smooth and productive. The “steadiness” in the pilot study is “a parameter that helps the three constituents of higher education to communicate to each other without ambiguity or say on a same frequency” and is very close to the factor “similarity”. Studies prove that “similarities” help group cohesion, improve performance and social relations etc. So to assume that the presence of like-mindedness or “similarity” or say “steadiness” in the field of higher education has the potential to decrease the amount of stress and to increase the productivity or outcome （successful policies, objective assessments, scholars, research, utility etc.）, is justified. And the assumption possesses the testable potential to study policy, assessment, implementation and other allied issues objectively. Many methods of social research and experimental designs are feasible for such study. A pilot study of a biography proves that the study of “similarity” in higher education for quality and assessment is viable and possesses the potential to produce better scholars, can enable the government to improve economy, can help to produce a strong next generation, can positively contribute to strengthen the social institutions and society and above all can bring a positive change in higher education that has been merely claimed for last many years. It is recommended that the proposed “similarity” may be studied in the field of higher education to help the government to formulate and execute policies based on social psychological evidence to enable the executors to best utilize their potential to help the recipients and learners to grow as per their full potential. 相似文献
15.
INTRODUCTION Multimedia wireless services require reliable high-bit-rate transmission over mobile radio channels. One of the challenges in designing such systems is the mitigation of fading propagation effects within the prescribed bandwidth and power limitations. Multi- ple-input and multiple-output (MIMO) techniques can be implemented to obtain a capacity gain in rich scattering environments without increasing the bandwidth or transmit power and/or to obtain the diversity gain to comba… 相似文献
16.
Many countries have language policies with the purpose of promoting or discouraging the use of a particular language or a set of languages. In a multilingual country, the official language(s) is always favored by the national language policy; in contrast, the minority languages, to some extent, are hindered in their development. This paper, by the case studies of minority languages in New Zealand, Netherlands, and China, illustrates the effects of these countries’ general language policies on their minority languages. Minority languages are seen as a part of cultural identity of minority groups. To protect their own culture and identity, they devote themselves to seek the equal right for their languages and cultures. In making language policies, the authorities should give respect and regard to the minority languages. 相似文献
17.
Within the framework of eco-feminist theory,this paper,taking Andrew Marvell’s "To His Coy Mistress" as a specific example,to explain how women and nature are marginalized and reduced to aphasia condition in literature.The paper presents a detailed analysis of the language of the poem,reveals men’s anthropocentric-andocentric attitudes toward nature and women and proposes the decentralization or the relation of "anotherness" as a possible way to reestablish a healthy culture(men)-nature(women) relationship. 相似文献
18.
(A)Every day we go to school and listen to teachers, and the teacherwill ask us some questions. Sometimes, the classmates will ask youropinions of the work of the class. When you are telling others in the classwhat you have found out about these topics, remember that they must beable to hear what you are saying. You are not taking part in a family con-versation or having a chat with friends——you are in a situation where alarge group of people will remain silent, waiting to hear what you have… 相似文献
19.
Pun is one of the most common figures of speech in English. It is the humorous use of a word in such a way as to suggest different meanings or applications or of words having the same or nearly the same sound but different meanings. Due to the comparative complexity of its formation, there are different ways of classification of English pun. And some special rhetorical effects can be achieved by the employment of English pun. 相似文献
20.
ZHUANG Hui-zhong DU Shu-xin WU Tie-jun 《浙江大学学报(A卷英文版)》2006,7(4):516-524
INTRODUCTION Path planning of mobile robots is one of the key issues in robotics research on the problem of a robot finding a collision-free path from beginning to goal in the presence of obstacles. Depending on the envi- ronment surrounding the robot, it can be classified as follows: (1) Path planning for static obstacles in com- pletely known environment; (2) Path planning for static obstacles in un- known or partially known environment; (3) Path planning for dynamic obstacles in com- p… 相似文献