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1.
T. Raghava Rao D. N. Rao B. Veerendra Kumar P. Aparanji K. Srinivas Rao R. Athota 《Indian journal of clinical biochemistry : IJCB》2003,18(1):29-34
Sensitization to ingested foods is a known fact and several food allergens have been characterized. It has been observed in
our survey that the people complained of allergic symptoms after consumption of the vegetableVigna sinensis. In this study, the experiments were carried to investigate the IgE antibody response against the green seed extract of vigna
sinensis in mice and found that the primary, secondary and tertiary immunization with or without adjuvant by different doses
induced a significant production of IgE antibodies. The presence of IgE antibodies in the mice sera were determined by passive
cutaneous anaphylaxis and enzyme linked immunosorbent assay. It was also confirmed that these allergens were found to be heat
resistant and shared a common epitope(s) with the other legume foods, as evidenced by the cross-reactive studies. 相似文献
2.
Menon BR Rathi MA Thirumoorthi L Gopalakrishnan VK 《Indian journal of clinical biochemistry : IJCB》2010,25(4):401-404
The study was designed to evaluate the hepatoprotective activity of ethanolic extract of Bacopa monnieri in acute experimental liver injury induced by Nitrobenzene in rats. The extract at the dose of 200 mg/kg body weight was
administered orally once every day for 10 days. The increased serum marker enzymes, Aspartate transaminase, Alanine transaminase
and alkaline phosphatase were restored towards normalization significantly by the extract. Significant increase in SOD, CAT
and GPx was observed in extract treated liver injured experimental rats. Histopathological examination of the liver tissues
supported the hepatoprotection. It is concluded that the ethanolic extract of Bacopa monieri plant possess good hepatoprotective activity. 相似文献
3.
In this study, we have examined the effect of linking of bloactive fragment of human IL-1β (163–171) or polytuftsin (PT, a
synthetic polymer of natural immunomodulator “tuftsin”) with synthetic peptides of HIV on the induction of immune response
to the synthetic peptides. A panel of synthetic peptides representing defined region of gp41, gp120 and gag were used as antigens.
Immunomodulators linked peptides (i.e. peptide-IL-1β or peptide-PT) or peptide dimers were employed for immunization in Balb/c
mice. Mice immunized with the peptide-immunomodulator develop effective T-cell proliferation,in vitro cytokine release and higher antibody production, but not with peptide dimers. We also found that peptide-immunomodulators
induced high level of IgG2a antibody production. Furthermore, there was a positive correlation between the levels of cytokine
(IL-2 & IFN-γ) and IgG isotype production. Thus it would appear that incorporation of IL-1β fragment or PT selectively enhances
the Th1 type response to these peptides and may therefore be important for virus neutralization and clearance. However, the
effect of IL-1β fragment was found to be more pronounced than polytuftsin. Such an approach may provide effective vaccination
against other infectious diseases. 相似文献
4.
P. J. Benny G. Muraleedhara Kurup K. Sreejith 《Indian journal of clinical biochemistry : IJCB》1997,12(2):108-113
The anticancerous drug isolated in our laboratory from estuarineAeromonas was characterised and is found to be an enzyme, L-asparaginase. The antileukaemic effect of this drug was studied in mice
by inducing leukaemia with Ehrlich ascites cell lines. It was compared with commercially available drug, Leunase, isolated
fromE. coli. The lipid profiles in mice during leukaemia and under treatment was studied. The decreased levels of cholesterol and increased
levels of triglycerides and phospholipids in serum, liver and kidney were observed in tumour bearing mice. Significant changes
in the above values were observed with enzyme therapy. It could bring some of the values to near normal level. L-asparaginase
fromAeromonas was found to be more effective. 相似文献
5.
R. L. Gupta Sundeep Jain V. Talwar H. C. Gupta P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》1998,13(2):92-97
After demonstrating that trifluoperazine (TFP) possesses invitro antitubercular activity against drug (single and multidrug) resistantMycobacterium tuberculosis, we initiated preliminary clinical studies in a few patients of tubercular lymphadenitis. Effect of TFP was assessed by testing
the antitubercular activity of the serum of patients receiving TFP in addition to regular therapy. Patients were divided into
two groups of 30 each. For ethical considerations, patients of both groups were treated initially for one month with antitubercular
therapy (ATT) consisting of isoniazid, rifampicin, ethambutol and pyrazinamide and TFP was tried for 15 days only. Patients
of group1 were given a single dose of TFP (5mg/day) daily from days 31 to 45 in addition to ATT, while those in group 2 received
ATT only. Assessment of the antitubercular activity of the serum (testedin vitro in Youmans and Karlson’s liquid medium) revealed that the serum of patients (collected on 45th day) of group1 (ATT+TFP treated)
possessed much higher antitubercular activity than that of group 2 (ATT only treated) patients. Clinical examination indicated
that overall improvement was seen much earlier in group1 (ATT+TFP) patients than in group 2 (ATT alone) patients. At the end
of the follow up period of 6 months with ATT from 46th day onwards to both groups, there were no side effects due to TFP.
Hematology and liver function tests were normal in both the groups. We suggest that TFP has good potential and therefore deserves
further studies either in combination with other drugs of ATT or as one of the drugs of ATT, for the treatment of tuberculosis
due to MDR strains to find a suitable effective dose without side effects. 相似文献
6.
P. Pavana S. Sethupathy S. Manoharan 《Indian journal of clinical biochemistry : IJCB》2007,22(1):77-83
The aim of the present study was to evaluate the antihyperglycemic and antilipidperoxidative effects of ethanolic seed extract
ofTephrosia purpurea (TpEt) in streptozotocin induced diabetic rats. Hyperglycemia associated with an altered hexokinase and glucose 6 phosphatase
activities, elevated lipid peroxidation, disturbed enzymatic and non-enzymatic antioxidants status were observed in streptozotocin
induced diabetic rats. Oral administration of “TpEt” at a dose of 300mg/kg bw showed significant antihyperglcemic and antilipidperoxidative
effects as well as increased the activities of enzymatic antioxidants and levels of non enzymatic antioxidants. We also noticed
that the antihyperglycemic effect of plant drug (TpEt) was comparable to that of the reference drug glibenclamide. Our results
clearly indicate that “TpEt” has potent antihyperglycemic and antilipidperoxidative effects in streptozotocin induced diabetic
rats and therefore further studies are warranted to isolate and characterize the bioactive antidiabetic principles from “TpEt”. 相似文献
7.
The anti oxidative effect of administration of 100 mg/kg bw and 200 mg/kg bw of the flower powder of Cassia auriculata (CFP) for 45 days to normoglycemic and diabetic rats (streptozotocin induced) was studied. Anti oxidative effect was not
observed in normoglycemic rats in the experiment. There was significant (P > 0.05) increase in the level of Thio Barbituric Acid Reactive Substances (TBARS), hydroperoxide and conjugated dienes and
significant (P > 0.05) decrease in the catalase, superoxide dismutase and glutathione peroxidase activities and in the level of ascorbic
acid, vitamin E and reduced glutathione in diabetic rats. The flower powder of Cassia auriculata significantly (P > 0.05) decreased the TBARS, hydroperoxide and conjugated dienes and increased the antioxidant enzymes (catalase, superoxide
dismutase and glutathione peroxidase) and non enzymic anti oxidants (ascorbic acid, vitamin E and reduced glutathione). The
antioxidatve effect of 200 mg/kg bw CFP was significantly (P > 0.05) better than 100 mg/kg bw CFP and the reference drugs (tolbutamide and metformin). The mode of action of CFP remains
to be elicited. 相似文献
8.
The objective of the present study was to evaluate hypolipidemic effect of methanolic extract of Celastrus paniculatus in experimentally induced hypercholesterolemic rats. Hypercholesterolemia was induced by feeding the animals with high fat
diet. Oral administration of methanolic seed extract (50%) of Celastrus paniculatus at the optimized dose of 65 mg/kg body weight, substantially reduced the plasma total cholesterol, triglycerides and LDL
cholesterol in comparison with induced hypercholesterolemic animal group and the results were comparable with the standard
hypocholesterolemic drug and almost similar to the control group. Atherogenic index and liver weight of treated animals also
showed significant decrease compared to the hypercholesterolemic animals. It substantially increased the HDL cholesterol level
as compared to control group. A significant increase in the activities of lipoprotein lipase and plasma LCAT enhanced hepatic
bile acid synthesis and thereby, increased degradation of cholesterol to neutral sterols. Furthermore, the activities of HMG-CoA
reductase, glucose 6-phosphate dehydrogenase and malate dehydrogenase were significantly reduced. Histological studies showed
less cholesterol deposits in the aorta of animals fed with seed extract of C. paniculatus compared to the induced hypercholesterolemic animals not given C. paniculatus supplement. 相似文献
9.
10.
Govinda Rao Duddukuri Y. Vasudeva Rao D. N. Rao Rao R Athota 《Indian journal of clinical biochemistry : IJCB》2001,16(1):89-94
It was reported earlier that intraperitoneal administration of honey had immunosuppressive activity on elicitation of allergen-specific
murine antibody response as evaluated by passive cutaneous anaphylaxis and double immunodiffusion methods. In this study,
the immunomudulatory effect of honey is evaluated by enzyme linked immunosorbent assay (ELISA) using ovalbumin as model allergen.
It was found that ovalbumin (OVA)-specific IgG antibody responses elicited with various doses of OVA were significantly suppressed
by rock bee honey (p<0.01). Honey was also found to have inhibited the production of OVA-specific IgM, IgA, IgG1, and IgG2b whereas that of IgG2a and IgG3 were not affected. Furthermore, honey also suppressed the OVA-specific total IgG antibody response in various inbred mice
with different genetic background. In addition, the suppressive activity of honey was examined in different groups of mice
by injecting honey at different time intervals, before and after immunization with OVA. The anti-OVA IgG antibody response
was suppressed significantly when honey was injected 12 hours prior/latter to OVA injection. These results confirm the suppressive
activity of honey on antibody response and suggest possible clinical application. 相似文献
11.
Dolly Jaiswal Prashant Kumar Rai Amit Kumar Geeta Watal 《Indian journal of clinical biochemistry : IJCB》2008,23(2):167-170
The present study deals with the evaluation of glycemic profile of aqueous extract of Cajanus cajan leaves in streptozotocin induced type 2 diabetic rats. Single oral administration of graded doses of aqueous extract of Cajanus
cajan leaves showed significant increment of 14.3 % in fasting blood glucose levels of normal rats. The sub diabetic and mild diabetic
models have also shown hyperglycemic effect from the same variable doses of the extract. The dose of 1000 mg/kg showed the
maximum rise of 17.1, 71.2 and 50.7 % in Blood glucose levels of normal, sub and mild diabetic rats respectively during glucose
tolerance test. The study of leaves was taken into consideration on the basis of earlier reported hypoglycemic activity of
Cajanus cajan seeds. However the results observed were found just opposite and therefore it may be useful in controlling hypoglycemia,
occasionally caused due to excess of insulin and other hypoglycemic drugs. 相似文献
12.
Amol D. Jadhav Bao Yan Rong-Cong Luo Li Wei Xu Zhen Chia-Hung Chen Peng Shi 《Biomicrofluidics》2015,9(3)
Microvalves with different actuation methods offer great integrability and flexibility in operation of lab-on-chip devices. In this work, we demonstrate a hydrogel-based and optically controlled modular microvalve that can be easily integrated within a microfluidic device and actuated by an off-chip laser source. The microvalve is based on in-channel trapping of microgel particles, which are composed of poly(N-isopropylacrylamide) and polypyrrole nanoparticles. Upon irradiation by a near-infrared (NIR) laser, the microgel undergoes volumetric change and enables precisely localized fluid on/off switching. The response rate and the “open” duration of the microvalve can be simply controlled by adjusting the laser power and exposure time. We showed that the trapped microgel can be triggered to shrink sufficiently to open a channel within as low as ∼1–2 s; while the microgel swells to re-seal the channel within ∼6–8 s. This is so far one of the fastest optically controlled and hydrogel-based microvalves, thus permitting speedy fluidic switching applications. In this study, we successfully employed this technique to control fluidic interface between laminar flow streams within a Y-junction device. The optically triggered microvalve permits flexible and remote fluidic handling, and enables pulsatile in situ chemical treatment to cell culture in an automatic and programmed manner, which is exemplified by studies of chemotherapeutic drug induced cell apoptosis under different drug treatment strategies. We find that cisplatin induced apoptosis is significantly higher in cancer cells treated with a pulsed dose, as compared to continuous flow with a sustained dose. It is expected that our NIR-controlled valving strategy will provide a simple, versatile, and powerful alternative for liquid handling in microfluidic devices. 相似文献
13.
Parvinder Kaur Sheenu Mathur Meenakshi Sharma Manisha Tiwari K. K. Srivastava Ramesh Chandra 《Indian journal of clinical biochemistry : IJCB》2001,16(2):195-198
In Ayurvedic medicine,Withania somnifera (Ashwagandha) is well known for its anti-stress activity. A passive rat experimental model, where the animals are subjected
to multiple stress of cold, hypoxia, restraint (C-H-R) has been developed to evaluate adaptogenic properties of various fractions
of W. somnifera root extracts. We have carried out extraction of roots of W. somnifera with water and further isolated one
of the active constituents called compound X and also tested its anti-stress activity in C-H-R model. The effect of administration
of W. somnifera water suspension (360 mg/Kg bw) and compound X (20mg/Kg bw) on the fall and recovery of colonic temperature
was noted. There was an increase of ≈38% and ≈54% in the time taken to attain Trec 23°C by rats given a single dose of fresh aqueous suspension and biologically active constituent (Compound X) respectively,
where as decrease in the recovery time to attain Trec 37°C is ≈13% and ≈33% respectively, as compared to control group. It is clear that rats treated with the fresh aqueous suspension
and compound X ofWithania somnifera, could withstand the multiple stress of C-H-R better than control group. 相似文献
14.
Shanmugam Velayuthaprabhu Arunachalam Chinnathambi Sulaiman Ali Alharbi Hidehiko Matsubayashi Govindaraju Archunan 《Indian journal of clinical biochemistry : IJCB》2017,32(3):347-352
To investigate the effects of antiphospholipid antibodies on establishment of pregnancy and changes in hormones such as estradiol-17ß (E2) and progesterone (P) levels in circulation. Hence, mice were immunized with human β2-Glycoprotein I (β2GPI) and the effect of these antibodies on fetuses weight, placental obsrvation, Serum levels of P and E2 in pregnant mice, hematological were observed. Immunization of mice with human β2-GPI resulted in elevated levels of antiphospholipid antibodies. The experimentally induced antiphospholipid syndrome mouse showed higher rate of fetal resorption, low number of viable fetuses, and “placental abnormalities”. In these animals, serum E2 and P levels were reduced significantly. In addition, the blood cell variation among APS induced and control mice were determined. No significant variations were observed in number of Red Blood Cell count, White Blood Cell count and Hemoglobin content, while platelet number was significantly reduced as compared to control. These results clearly demonstrate that human β2-GPI might be involved in causing gestational failure in APS by exerting their effect on serum hormones. 相似文献
15.
Dinesh C. Sharma Deepa Chandiramani Manminder Riyat Praveen Sharma 《Indian journal of clinical biochemistry : IJCB》2007,22(2):123-128
A number of preparations are available in Ayurved for treatment of anemia and iron deficiency. This study was designed to
evaluate efficacy of some of them. Six most commonly used Ayurvedic iron containing preparations (Navayasa Curna, Punarnavadi Mandura, Dhatri Lauha, Pradarantaka Lauha, Sarva-Juara-Hara Lauha and Vrihat Yakrdari Lauha) were given in a dose of 250 mg b.d. for 30 days to six groups of iron deficient anemic patients; each group consisting of
20 patients. A control group was given Allopathic preparation—Irex-12, (containing—ferrous fumarate, vitamin C, folic acid
and vitamin B12); 1 capsule daily for 30 days for comparison. All hematological and iron parameters were determined before and after completion
of treatment. The results showed that there was statistically significant rise (p<0.001) in all of them—Hb, PCV, TRBC, MCV,
MCH, MCHC and plasma iron, percent saturation and plasma ferritin. Total iron binding capacity decreased significantly (p<0.001).
The response of most of Ayurvedic preparations was better than Allopathic preparation and there was no side effect as observed
with iron salts The Hb regeneration rate was 0.10 g/dl/day for Allopathic preparation; while it was above this value for all
Ayurvedic preparations exceptPradarantaka Lauha which was least effective.Sarva-Juara-Hara Lauha was the drug of choice as Hb regeneration with it was highest 0.16 g/dl/day. Upon analysis of Ayurvedic drugs, these results
were found to be consistent and correlated with iron content of the preparation. 相似文献
16.
P Renuka Devi S Krishna Kumari C Kokilavani 《Indian journal of clinical biochemistry : IJCB》2007,22(1):143-147
The effect of the oral administration ofVitex negundo leaf extract on the levels of enzymic and non-enzymic antioxidants were studied in the adjuvant induced arthritic (AIA) rats
The levels of antioxidant enzymes such as SOD, CAT, GPx, G6PD, GSH and Vit-C were estimated in various groups of the experimental
rats. It was observed that the antioxidant enzyme levels in the AIA were significantly low when compared to normal rats. A
significant decrease in enzymic antioxidant—SOD, CAT, GPx, G6PD and non-enzymic antioxidant—GSH, Vit-C were observed in the
liver of AIA rats compared to the normal rats. These results suggest that the leaf extract ofVitex negundo possesses antioxidant activity. 相似文献
17.
Jyoti Sethi Sushma Sood Shashi Seth Anjana Talwar 《Indian journal of clinical biochemistry : IJCB》2004,19(2):152-155
Ocimum sanctum leaves have been traditionally used in treatment of diabetes mellitus. Dietary supplementation of fresh tulsi leaves in a
dose of 2 gm/kg BW for 30 days led to significant lowering of blood glucose levels in test group. Intake ofOcimum sanctum also led to significant increase in levels of superoxide dismutase, reduced glutathione and total thiols, but marked reduction
in peroxiodised lipid levels as compared to untreated control group. The leaves were found to possess both superoxide and
hydroxyl free radical scavenging action. The present observations establish the efficacy ofOcimum sanctum leaves in lowering blood glucose levels and antioxidant property appears to be predominantly responsible for hypoglycemic
effect. 相似文献
18.
Gacche RN Shaikh RU Chapole SM Jadhav AD Jadhav SG 《Indian journal of clinical biochemistry : IJCB》2011,26(3):303-308
The study was designed to evaluate the antioxidant activity and effect of Cymbopogon martinii (Roxb.) Wats. (Poaceae) leaves on the activity of monoamine oxidase and kinetics of enzyme inhibition. Ethanol extract of
C. martinii and rat brain mitochondrial monoamine oxidase preparation ware used to study the kinetics of enzyme inhibition using double
reciprocal Lineweaver–Burk plot. The DPPH was used as a source of free radical to evaluate antioxidant potential. It is observed
that, the ethanolic extract of C. martinii inhibits the monoamine oxidase activity with competitive mode of inhibition. The V
max (0.01 mM/min) remained constant while, K
m varied from 21.00 ± 1.1, 43.33 ± 1.5 and 83.33 ± 1.4 mM for 100–500 μg/ml concentration of C. martinii. The K
i values were calculated to be 90.00 ± 0.87, 75.00 ± 0.69, 68.18 ± 0.68 μg for 100–500 μg/ml concentration of C. martini. It also shows a significant DPPH (1,1-diphenyl-2-picryl hydrazine) radical scavenging (IC50 = 0.34 ± 0.05 mg/ml) and reducing activity (IC50 = 0.70 ± 0.22 mg/ml). The C. martini can be considered as a possible source of MAO inhibitor used in the treatment of depression and other neurological disorders. 相似文献
19.
Y. K. Murali Ramesh Chandra P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》2004,19(2):202-204
Water extract of dry fruits ofTerminalia chebula (Hindi-Harda, Telugu-Karakkaya) at a dose of 200 mg/kg body weight improved the glucose tolerance as indicated by 44% of
reduction in the peak blood glucose at 2nd hour in glucose tolerance test in diabetic (streptozotocin induced) rats. Treatment of diabetic rats with an initial fasting
blood glucose of 253±9.4 mg/dl daily once with the water extract (200 mg/kg) for two weeks brought down the fasting blood
glucose to 123±8.4 mg/dl which is only slightly above the normal value. These results indicate that water extract of Terminalia
chebula improves glucose tolerance and brings down fasting blood glucose in diabetic rats. 相似文献
20.
肿瘤多药耐药 (multidrugresistance ,MDR)是临床化疗成功最为严重的障碍 .首先阐明了新拓扑异构酶II抑制剂沙尔威辛对MDR肿瘤细胞直接的细胞毒性作用及下调mdr 1基因和P 糖蛋白的作用 .沙尔威辛能有效杀伤MDR细胞株 ,如K5 62 A0 2 ,KB VCR和MCF 7 ADR细胞 ,其杀伤能力与对相应亲本细胞相当 ,而明显强于几种临床常用的抗癌药物 .沙尔威辛下调mdr 1基因和P 糖蛋白的表达 ,但并不影响MRP和LRP基因 .其次 ,揭示了转录因子c jun的激活 ,在沙尔威辛下调K5 62 A0 2细胞内mdr 1基因表达及诱导凋亡过程中起着关键作用 .沙尔威辛增加K5 62 A0 2细胞的c jun表达明显早于其减少mdr 1基因的表达 ;c jun反义寡核苷酸消除沙尔威辛升高c jun蛋白、下调mdr 1基因表达的作用 .沙尔威辛还促进JNK和c jun磷酸化并增强转录因子AP1的DNA结合活性 .此外 ,c jun反义寡核苷酸还抑制沙尔威辛的凋亡诱导和细胞毒性作用 .最后 ,进一步研究发现沙尔威辛本身不引起MDR表型 .成功建立了对沙尔威辛具有 8 91倍耐药的A5 4 9 SAL细胞株 .该细胞株对抗代谢药产生 6.70倍的耐药 ,但对多种其他天然来源的抗肿瘤药物、烷化剂以及铂类化合物则缺乏交叉耐药性 . 相似文献