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1.
High blood pressure (hypertension) is implicated in the development of atherosclerosis. Blood vessels are constantly subjected to stretch due to blood pressure and changes in stretch usually instigate adaptive vascular remodeling, including abnormal growth and proliferation of vascular smooth muscle cells (VSMCs) as well as extracellular matrix (ECM). In this experiment, we used bovine aortic endothelial cells and smooth muscle cells (EC-SMC) co-cultured ePTFE vascular grafts subjected to normal atmospheric pressure (as a control), and 100 mmHg hydrostatic pressure for 7 d. The increase of cell layer thickness was observed. When measured, the cell layer thickness increased by 116.2%. The increase of collagen (Type IV) synthesis was also observed in the immunohistochemistry assay. When stained with toluidine blue, the cells showed metachromatic phenomenon.  相似文献   

2.
目的:探测细胞氧化低密度脂蛋白(LDL)过程中人α-防御素-1(HNP-1)基因的表达水平并构建人HNP-1的克隆载体。方法:提取人单核细胞系(THP-1)的总RNA,逆转录聚合酶链反应(RT-PCR)法获得cDNA,采用pBS-T克隆HNP-1。结果:用RT-PCR在THP-1细胞总RNA中扩增出一条285 bp的DNA片段,与HNP-1 cD-NA片段大小一致。重组质粒经PCR和测序鉴定获HNP-1基因克隆。另外,LDL可诱导THP-1细胞HNP-1的mR-NA表达增加。结论:HNP-1可能参与LDL的细胞氧化过程,成功构建HNP-1克隆载体将为进一步亚克隆到原核及真核的表达载体提供了基础。  相似文献   

3.
ADMA与动脉粥样硬化研究进展   总被引：1，自引：0，他引：1  
动脉粥样硬化等多种心血管疾病,表现为血管内皮功能紊乱,并伴有非对称性二甲基精氨酸 （ADMA,内源性一氧化氮合酶抑制物）血浓度的升高,ADMA可能是一种重要的致动脉粥样硬化因子。 ADMA通过抑制一氧化氮合酶（NOS）、致炎与诱导氧化应激等机制影响内皮细胞、平滑肌细胞、单核细胞及血小板等多种细胞功能,从而促进动脉粥样硬化发生与发展。  相似文献   

4.
目的:观察匹伐他汀钙对高胆固醇血症患者外周血管的影响。创新点:首次在国内发现匹伐他汀钙能够改善高胆固醇血症患者肱动脉和颈动脉血管内皮功能而且延缓其动脉粥样硬化发展,并首次证实改善内皮功能是匹伐他汀钙延缓其动脉粥样硬化发展的重要原因。方法:按照入选排除标准,选取本院高胆固醇血症患者(HC),完成超声心动图检查的40例。根据剂量不同,分为两个剂量组:1 mg剂量组20例(男性5例,女性15例,平均年龄(55.20±8.35)岁),2 mg剂量组20例(男性9例,女性11例,平均年龄(57.56±6.09)岁)。访视结束后完成超声心动图检查的HC组36例,两个剂量组分别有2人失访。治疗后1 mg剂量组18例(男性3例,女性15例,平均年龄(56.00±7.85)岁),2 mg剂量组18例(男性7例,女性11例,平均年龄(57.79±6.46)岁)。选择本院同期体检中心30例正常人作为对照(年龄和性别均与病例组匹配,男性14例,女性16例,平均年龄(54.94±6.90)岁)。所有研究对象,均经隔夜禁食12~14小时,次日清晨抽取空腹肘静脉血,测定临床生化指标。采用Sequia512彩色多普勒超声诊断仪,应用高分辩率外周血管超声技术,检测HC治疗前后肱动脉血流介导性舒张功能(FMD)、颈动脉结构和功能。结论:经匹伐他汀钙治疗8周后,高胆固醇血症患者血管功能明显改善,表现为FMD升高,僵硬度减小;颈动脉僵硬度和内中膜厚度(IMT)延缓进展与其内皮功能改善密切相关。  相似文献   

5.
血管紧张素-（1—7）是AngII的内源性拮抗因子，具有保护血管内皮细胞抗血管平滑肌细胞增殖与迁移等作用，从而发挥抗动脉粥样硬化效应。探讨血管紧张素-（1—7）的生理功能及其在抗动脉粥样硬化中的作用，有助于进一步揭示ACEI及ATl受体阻断剂的抗动脉粥样硬化机制，并有可能为防治动脉粥样硬化等疾病提供新的有效思路。  相似文献   

6.
目的观察川芎嗪酯类衍生物对H2O2引起的体外培养人脐静脉内皮细胞氧化性损伤的保护作用,并初步探讨川芎嗪双酯衍生物在家兔动脉内皮氧化损伤中的保护作用。方法H2O2引起体外培养人脐静脉内皮细胞及家兔动脉内皮氧化性损伤,观察川芎嗪双酯衍生物对内皮细胞氧化损伤的保护作用。结果在各试验浓度,大多数川芎嗪双酯衍生物对H2O2引起体外培养人脐静脉内皮细胞氧化性损伤有明显的保护及促增殖作用,A6在较高浓度对家兔动脉内皮的氧化损伤有保护作用。结论川芎嗪酯类衍生物对H2O2引起的体外培养人脐静脉内皮细胞氧化性损伤有明显保护作用,其中以A15效价最高,在其作用机制进一步研究中值得注意。  相似文献   

7.
本文的目的是研究半多孔内皮细胞的包膜(SPEUs)的效应 以PTMEG1000为基础的SPEU的多孔性为51.58%(±5.86%) 与天然脉管相比较,例如与一头6个月小牛胸部的上部下来的主动脉大血管相比较,SPEU的拉伸强度是足够强的 脉管的典型脉动压力服从静脉管压力(1.5～2%Δd/d),此压力小于动脉管压力(9～15%Δd/d) 在种植EC之前,观察了在SPEUs上培育的人皮的成纤维细胞(HDF) SPEU的DNA数量与HDF在一天之内种植;有71.9%(±11.01%)细胞被种植在SPEUs上 LDH试验表明:在有控制的和实验群数据之间没有显著的差别,说明没有细胞毒素 因此,EC在SPRUs上具有良好的粘附性,这是所期望的,而应用胶原实现了将EC种植在半微孔的SPEUs上的表面改善  相似文献   

8.
Homoharringtonine (HHT) has currently been used successfully in the treatment of acute and chronic myeloid leukemias and has been shown to induce apoptosis of different types of leukemic cells in vitro. Emerging evidence suggests that angiogenesis may play an important role in hematological malignancies, such as leukemia. How ever, whether HHT can relieve leukemia by anti-angiogenesis is still unknown. We investigated the anti-angiogenesis potential of HHT with the human umbilical vein endothelial cell line (ECV304) and leukemic cell line (K562) in vitro. Cellular proliferation was determined by MTT assay and apoptosis was analyzed by flow cytometry. The mRNA expression of vascular endothelial growth factor (VEGF) was assessed by RT-PCR and VEGF protein production was detected by Western blot. Inhibition of cell proliferation and induction of apoptosis by HHT were discovered in ECV304 cells, and appeared in a dose- and time-dependent manner. Also, treatment with HHT caused down-regulation of VEGF mRNA expression in K562 cells in similar dose- and time-dependent manner and inhibition of VEGF protein production in K562 cells in response to the enhancing concentration of HHT. The results demonstrated that HHT could also induce apoptosis in endothelium and down-regulate VEGF expression in K562 cells. In conclusion, we believe HHT has anti-angiogenesis potential and speculate that HHT might exert its anti-leukemia effects via reduction of angiogenesis.  相似文献   

9.
INTRODUCTION Homoharringtonine (HHT) is a cephalotoxin alkaloid with anti-leukemic activity and had been used successfully in the treatment of acute and chronic myeloid leukemias (O払rien et al., 1995; 1999; Feldman et al., 1992). The principal mecha-nism of action by HHT is the inhibition of protein synthesis in a dose- and time-dependent manner by binding to ribosome and inhibiting polypeptide chain elongation (Tujebajeva et al., 1989; Zhou et al., 1995). HHT had been shown to indu…  相似文献   

10.
目的探讨CD40-CD40配体相互作用对体外培养的人脐静脉内皮细胞(HUVEC)增殖及迁移的影响。方法采用Ⅱ型胶原酶消化法培养人脐静脉内皮细胞,以3H-胸腺嘧啶脱氧核苷(3H-TdR)、3H-亮氨酸(3H-Leu)掺入法分别测定HUVEC增殖,内皮细胞的迁移采用琼脂糖凝胶刮取法,倒置显微镜观察。结果CD40-CD40配体相互作用能明显促进HUVEC3H-TdR、3H-Leu的掺入,两者具有时间、剂量依赖性,CD40-CD40配体相互作用随CD40L浓度的增加及刺激时间延长(30h内),能明显增加内皮细胞迁移率,an-ti-CD40单克隆抗体能明显抑制上述效应。结论CD40-CD40配体相互作用能促进内皮细胞增殖、迁移。  相似文献   

11.
血管内膜增生是动脉粥样硬化和血管成形术后再狭窄等心血管疾病共同的生理特征,与血管重塑关系密切,防止内膜增生及术后再狭窄一直是国内外研究的重要课题。为从不同水平研究血管内膜增生发病的机制,给临床针对病因治疗提供依据,本文将从内皮损伤与功能障碍、脂质沉积、炎症反应、血管平滑肌细胞向内膜迁移、增殖及合成大量细胞外基质、基因改变等多种机制的研究方向,总结近些年来的研究成果,同时指出未来将在基因水平（如IGF-1 mRNA）、细胞生物学水平（如VSMC）及分子生物学水平（如内皮抑素、CRP）等各种研究方面有望成为新的研究突破点。  相似文献   

12.
Objective: To investigate the relationship between the expression of endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) and angiogenesis in primary astrocytoma. Methods: Thirty-seven primary astrocytomas and 4 astrocytic hyperplasia samples were collected and divided into three groups according to histological grade. The expression of eNOS, VEGF and factorⅧrelated antigen (FⅧRAg) were assayed by immunohistochemistry. Microvascular density was assessed by FⅧRAg immunoreactivity. The intensity of immunoreactivity was graded according to the percentage of positive tumor cells. Results: No eNOS and VEGF were expressed in the astrocytes and vascular endothelium in astrocytic hyperplasia. The expression of eNOS or VEGF was light in low-grade astrocytoma and strong in glioblastoma. eNOS expression in astrocytoma was very positively correlated with VEGF. eNOS and VEGF expression in anaplastic astrocytoma was median in contrast to the low grade astrocytoma and glioblastoma. Lower microvascular density was found in low grade astrocytoma than that in higher grade malignant ones. The expressions of eNOS and VEGF were correlated with microvascular density and tumor malignancy. Conclusion: This finding suggests that eNOS and VEGF may have cooperative effect in tumor angiogenesis and play an important role in the pathogenesis of primary astrocytoma.  相似文献   

13.
To examine the effects of co-culture with bone marrow mesenchymal stem cells on expansion of hematopoietic stem/progenitor cells and the capacities of rapid neutrophil engraftment and hematopoietic reconstitution of the expanded cells, we expanded mononuclear cells (MNCs) and CD34+/c-kit+ cells from mouse bone marrow and transplanted the expanded cells into the irradiated mice. MNCs were isolated from mouse bone marrow and CD34+/c-kit+ cells were selected from MNCs by using MoFlo Cell Sorter. MNCs and CD34+/c-kit+ cells were co-cultured with mouse bone marrow-derived mesenchymal stem cells (MSCs) under a two-step expansion. The expanded cells were then transplanted into sublethally irradiated BDF1 mice. Results showed that the co-culture with MSCs resulted in expansions of median total nucleated cells, CD34+ cells, GM-CFC and HPP-CFC respectively by 10.8-, 4.8-, 65.9- and 38.8-fold for the mononuclear cell culture, and respectively by 76.1-, 2.9-, 71.7- and 51.8-fold for the CD34+/c-kit+ cell culture. The expanded cells could rapidly engraft in the sublethally irradiated mice and reconstitute their hematopoiesis. Co-cultures with MSCs in conjunction with two-step expansion increased expansions of total nucleated cells, GM-CFC and HPP-CFC, which led us to conclude MSCs may create favorable environment for expansions of hematopoietic stem/progenitor cells. The availability of increased numbers of expanded cells by the co-culture with MSCs may result in more rapid engraftment of neutrophils following infusion to transplant recipients. Project supported by NIH-Blood, Heart & Lung (National Institute of Health, USA, IR 01 4L70593-01) and Zhejiang Provincial Science Foundation (No. 011103397), China  相似文献   

14.
目的：探讨辛伐他汀对外源性制备的糖基化终末产物损伤大鼠离体胸主动脉环内皮依赖性舒张功能的影响及其机制。方法：按文献方法制备糖基化终末产物,采用外源性糖基化终末产物（AGE-BSA）孵育大鼠离体胸主动脉环90min诱导血管内皮功能的损伤,并观察辛伐他汀、超氧化物歧化酶和L-精氨酸对糖基化终末产物致的血管内皮依赖性舒张功能损害的影响。结果：外源性糖基化终末产物孵育大鼠离体胸主动脉环90min,明显抑制乙酰胆碱诱导的内皮依赖性血管舒张反应（endothelium-dependent relaxation,EDR）。但对硝普钠诱导的内皮非依赖性血管舒张反应没有影响。辛伐汀（0．05,0．1,0．2μmol／L）与AGE-BSA共同孵育血管环90min,浓度依耐性地改善AGE-BSA对血管内皮依赖性舒张功能的损害。氧自由基清除剂超氧化物歧化酶（superoxide dismutase,SOD,200U/mL）也可逆转AGE—BSA对内皮依赖性血管舒张反应的损害,而L-精氨酸（L-arginine,L-Arg,3mmol／L）却只有部分保护作用。而且,辛伐他汀也能取消SOD抑制剂二乙基二硫氨甲酸酯（diethyldithiocarbamate,DETC;10μmol／L）．诱导产生内源性氧自由基所致的血管内皮依赖性反应的抑制。结论：辛伐他汀能取消AGE-BSA对大鼠离体胸主动脉环血管内皮依赖性舒张反应的抑制。该保护作用可能与其抗氧化作用有关。  相似文献   

15.
Objective:To determine the effect of steroidogenic acute regulatory protein(StAR) overexpression on the levels of adenosine triphosphate(ATP)-binding cassette transporter A1(ABCA1) and ATP-binding cassette transporter G1(ABCG1) in an endothelial cell line(bEnd.3).Methods:The StAR gene was induced in bEnd.3 cells with adenovirus infection.The infection efficiency was detected by fluorescence activated cell sorter(FACS) and fluorescence microscopy.The expressions of StAR gene and protein levels were detected ...  相似文献   

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18.
Background:Bone marrow mesenehymal stem cell(MSC)transplantation is a promising strategy in the treatment of myocardial infarction(MI).However,the time for transplanting cells remains controversial.The aim of this study was to find an optimal time point for cell transplantation.Methods:MSCs were isolated and cultured from Sprague-Dawley(SD) rats.MI model was set up in SD rats by permanent ligation of left anterior descending coronary artery.MSCs were directly injected into the infarct berder zone at 1 h,1 week and 2 weeks after MI,respectively.Sham-operated and MI centrel groups received equal volume of phosphate buffered saline(PBS).At 4 weeks after MI,cardiac function Was assessed by echocardiography;vessel density Was analyzed on hematoxylin-eosin stained slides by light microscopy;the apoptosis of cardiomyocytes Was evaluated by terminal deoxynucleotidy1 transferase-mediated dUTP nick end-labeling(TUNEL) assay;the expressions of proteins were analyzed by Western blot.Results:MSC transplantation improved cardiac function.reduced the apoptosis of cardiomyocytes and increased vessel density.These benefits were more obvious in l-week group than in 1-h and 2-week groups.There are more obvious increases in the ratio of bc1-2/bax and the expression of vascular endothelial growth factor(VEGF)and more obvious decreases in the expression of cleaved-caspase-3 in 1-week group than those in other two groups.Conclusion:MSC transplantation was beneficial for the recovery of cardiac function.MSC transplantation at l week post-MI exerted the best effects on increases of cardiac function,anti-apoptosis and angiogenesis.  相似文献   

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20.
Objective: To explore the effects of cytomegalovirus (CMV) infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods: Endothelial cells (ECs) were cultured and stimulated by a variety of factors: A, normal control group; B, inactivated human cytomegalovirus (HCMV) infection group; C, HCMV infection group; D, HCMV supematant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 (ICAM-1) and major histocompability complex (MHC) class Ⅰ and class Ⅱ antigens was detected by flow cytometry (FCM) and immuno-histochemistry. Results: We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D (P>0.05). Although the expression levels of ICAM-1 were not significantly different between groups C and E (P>0.05), the ICAM-1 expression in these two groups was significantly higher than that in group A (P<0.05). ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E (P>0.05). Human leucocyte antigen (HLA)-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant dif-ferences of HLA-ABC and HLA-DR expression among groups A, B and D (P>0.05). However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported (P<0.05). Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C (P<0.05). Conclusion: CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection.  相似文献   

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