共查询到20条相似文献，搜索用时 125 毫秒
1.
Li-rong SHEN Yi-ran WANG Liang ZHAI Wen-xiu ZHOU Liang-liang TAN Mei-lu LI Dan-dan LIU Fa XIAO 《Journal of Zhejiang University. Science. B》2015,16(2):155-166
目的:为蜂王浆主蛋白1(MRJP1)的快速检测和鉴别提供科学依据,为蜂王浆的质量控制提供技术支持。创新点:首次比较了MRJP1特异性多克隆抗体与MRJP1重组表达蛋白多克隆抗体对王浆主蛋白家族的免疫反应差异,验证了蜂王浆中MRJP1蛋白降解与保温时间的相关性,建立了以MRJP1作为蜂王浆新鲜度生物标志物的快速检测方法。方法:通过蜂王浆主蛋白家族蛋白的氨基酸序列同源性分析,筛选出MRJP1的特异性多肽区域,进行人工合成,免疫兔子后取血清制备成特异性多克隆抗体。用蛋白质印迹法(Western blot)检测了MRJP1特异性多克隆抗体与MRJP1重组表达蛋白多克隆抗体对王浆主蛋白家族的免疫反应。以新鲜蜂王浆为对照品,用MRJP1特异性抗体酶联接免疫吸附剂测定(ELISA)法和变性电泳胶灰度扫描法分别测定保温(40°C)7~49天的蜂王浆中MRJP1含量的变化,并进行了相关性分析。结论:MRJP1的特异性抗体对MRJP1蛋白具有专一的免疫识别特性,可特异性地检测代表蜂王浆新鲜度的MRJP1含量变化,并鉴别蜂王浆的真伪。 相似文献
2.
金淑华 《赤峰学院学报(自然科学版)》2012,(1):52-53
目的:原发性胆汁肝硬化(PBC)与抗线粒体抗体、抗核点抗体、抗早幼粒细胞白血病蛋白抗体等多种抗体相关.自免肝抗原谱中的这些抗体是PBC患者血清学检测的标志,也有助于鉴别原发性胆汁性肝硬化(PBC)和自身免疫性肝炎(AIH).结果:应用欧蒙印迹法对499例不同类型血清进行检测,其中150例PBC患者血清中有93%的标本至少可检测到AMA-M2、M2-3E、gp210、SP100、PML中的一种抗体,相对于病毒性肝炎组和本院职工健康体检组其特异性为99%.LKM-1、LC-1、SLA/LP抗体为AIH的血清学标志,其特异性几乎为100%.结论:自免肝抗原谱中检测AMA-M2、M2-3E、gp210、SP100、PML、LKM-1、LC-1、SLA/LP和R0-52九种自身抗体对于PBC的诊断及PBC和AIH的鉴别诊断提供重要的实验依据. 相似文献
3.
目的:原核表达并纯化大型溞胆碱酯酶(ChE),制备鼠抗大型溞ChE多克隆抗体,并对抗体的适用性进行研究。创新点:首次通过原核表达获得了大型溞重组ChE蛋白,通过免疫小鼠获得了高效价、高特异性的多克隆抗体,通过样品检测证明了抗体的适用性。方法:利用聚合酶链式反应(PCR)技术获得大型溞ChE基因编码序列,并将其亚克隆至原核表达载体p ET-29a(+),构建重组表达质粒p ET-ChE,用异丙基硫代半乳糖苷(IPTG)诱导重组蛋白的表达;对表达产物进行聚丙烯酰胺凝胶电泳(SDS-PAGE)和酶活性检测,并对蛋白进行纯化(图4);使用纯化蛋白免疫BALB/c小鼠,制备多克隆抗体(图5),用酶联免疫吸附分析法(ELISA)对处于三唑磷和低温胁迫下以及经反复冻融处理的大型溞体内的ChE含量变化进行检测(图7、表6和7),以评价抗体的可适用性。结论:获得了高纯度的ChE重组蛋白;免疫小鼠后,获得了特异性强、高效价的抗体;通过对处于三唑磷和低温胁迫下以及经过反复冻融处理的大型溞体内的ChE含量的检测,证明了抗体的适用性。 相似文献
4.
本试验以偶氮法将克伦特罗连接到自身全血清和血红蛋白上制成佐剂苗分别免疫兔。免疫后用间接抑制ELISA、间接ELISA检测各组抗体特异性抗克伦特罗效价。结果表明,各组兔血清均含有抗克伦特罗的特异性抗体,显示自身全血清和血红蛋白能作载体,提示自身全血清因组分复杂作载体具有一定优越性。 相似文献
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卵黄抗体(Ig Y)是指用特定的抗原免疫产蛋鸡后,从免疫鸡蛋中获得的针对免疫抗原产生的特异性抗体。其制作简单,价格相对低廉,在疾病诊断、治疗、预防等方面有着广泛的应用。本文就卵黄抗体在病毒性疾病预防、治疗与检测中的应用进行相关的探讨和分析。 相似文献
6.
周跃 《淮北职业技术学院学报》2014,(5):140-141
自身免疫性溶血性贫血（autoimmune hemolytic anemia,AIHA）是血液科患者输血治疗深感棘手的问题之一,由于患者体内自身抗体或自身抗体掩盖下同种抗体的存在,导致临床输血复杂而危险。本文结合3例 AIHA 患者的抗球蛋白试验、血型鉴定、抗体筛查、抗体特异性鉴定及交叉配血相关实验室检测,探讨了选择合适的血液制品,保障患者输血安全的重要性。 相似文献
7.
目的:根据广州市海珠区2007-2011年麻疹血清抗体检测结果,分析麻疹发病的性别、年龄与季节分布。方法:采用酶联免疫吸附试验(ELISA)检测麻疹IgM抗体,整理总结历年检测结果。结果:2007-2011年共收集到疑似病例血清409例,IgM抗体阳性病例153例,阳性率37.41%。春季阳性率高(40.53%),IgM抗体阳性年龄为3个月-40岁,8个月龄组阳性率最高(56.86%)。结论:麻疹IgM抗体的检测是快速确诊病例主要方法之一,冬春季仍是麻疹发病的高峰时段,1岁以下儿童是高发人群。 相似文献
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以国际抗体验证工作组发表的关于验证抗体特异性策略的提议为基础,介绍抗体检测和特异性验证的五大支柱策略,系统性解答学生在学习单克隆抗体制备内容时的疑惑。 相似文献
9.
《河南职业技术师范学院学报(职业教育版)》2021,(1)
制备并鉴定针对重金属镉离子的小鼠多克隆抗体.选取异硫氰酸苄基乙二胺四乙酸(ITCBE)做金属螯合剂,偶联Cd~(2+)及载体蛋白BSA、OVA,制备人工抗原并鉴定,配合佐剂乳化后以高、中、低剂量分别免疫Balb/C小鼠,以获得较好的抗重金属镉离子小鼠多克隆抗体血清.采用酶联免疫吸附法(ELISA)测定所获抗体的效价、敏感度、特异性.结果显示:小鼠抗血清效价均在10~(-3)～10~(-4),中剂量抗原免疫组小鼠抗体血清更加稳定、特异性强,利用该组多抗建立间接竞争ELISA(icELISA)标准曲线,M-3号小鼠敏感性最强,回归方程为y=0.1123x-0.1638,IC_(50)质量浓度为368.70μg/L,检测限质量浓度为25.53μg/L,该抗体除与汞离子螯合物的交叉反应率为72.58%之外,与Cu~(2+)、Zn~(2+)、Pb~(2+)、Cr~(3+)、Mo~(6+)、Fe~(3+)、Co~(2+)均无交叉反应.获得了能用于镉离子免疫检测的高效价Cd~(2+)多抗,为检测产品的开发打下基础. 相似文献
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抗精子抗体检测诊断不孕症的临床价值 总被引：1，自引：0，他引：1
目的:检测不孕症和患者血清中抗精子抗体含量(包括总的抗精子抗体,抗精子抗体的IgG和IgA型)并计算出不孕症患者血清中抗精子抗体的阳性率.方法:采用酶联免疫吸附试验(ELISA)的方法检测76例不孕症患者及70例正常生育对照组血清中的抗精子抗体.结果:不孕症患者组血清中抗精子抗体阳性率为28.94%,明显高于正常生育者(抗精子抗体的阳性率为5.7%,P<0.01)并且,以IgG型抗体增高明显,IgA型抗体无显著性差异.结论:抗精子抗体是免疫性不孕的重要因素,ELISA方法定量检测抗精子抗体IgG型对诊断不孕症有一定价值. 相似文献
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宋艳琴 《襄樊职业技术学院学报》2012,11(4):42-43
目的观察胸腔置管引流术与间断性胸水穿刺术两种方法治疗结核性胸腔积液的疗效。方法大量结核性胸腔积液患者48例,随机分为胸腔置管引流术治疗组24例和间断性胸水穿刺术组24例,比较并发症发生率及其疗效。结果胸腔置管引流术组有效率明显高于胸腔穿刺术组;胸腔置管引流术组胸水吸收较快、并发症较少,二者有显著统计学意义（P〈0.05）。结论胸腔置管引流术治疗结核性胸腔积液并发症少,住院治疗时间短,是比较理想的辅助治疗方法。 相似文献
12.
Zhao-liang Xin Xiao-kang Wu Jian-rong Xu Xi Li 《Journal of Zhejiang University. Science. B》2010,11(7):516-523
Objective: To assess if arachnoid cells have the capability to present antigen and activate T-lymphocytes after stimulation by bloody cerebrospinal fluid (CSF), and to illuminate the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage (SAH). Methods: Arachnoid cells were cultured, characterized, and examined by immunofluorescence for the basal expression of human leukocyte antigen-DR (HLA-DR). Expression of HLA-DR, after co-culturing arachnoid cells in vitro with bloody CSF, was investigated by immunofluorescence and flow cytometry (FCM). The variation of arachnoid cells' ultrastructure was observed by transmission electron microscope (TEM). Arachnoid cells were co-cultured with peripheral blood mononuclear cells (PBMCs). The content of soluble interleukin-2 receptor (sIL-2r) in culture medium was detected by enzyme-linked immunosorbent assay (ELISA). Results: (1) Arachnoid cells were successfully cultured for many passages. The immunofluorescent staining was positive for HLA-DR in over 95% of the human arachnoid cells. The punctate HLA-DR was distributed in cytoplasm and not in the karyon. (2) After co-culturing arachnoid cells in vitro with bloody CSF, numerous particles with strong fluorescence appeared in the cytoplasm on Day 6. On Day 8, the quantity of particles and fluorescent intensity were maximal. FCM showed that the percentage of HLA-DR expressing cells was (2.5±0.4)% at the first 5 d, increasing to (60.8±3.6)% on Day 7. (3) After co-culturing arachnoid cells in vitro with bloody CSF, many lysosome and secondary lysosome particles were present in the cytoplasm. Hyperplasia of rough endoplasmic reticulum and enlarged cysts were observed, with numerous phagocytizing vesicles also observed at the edge of the arachnoid cells. (4) Arachnoid cells stimulated by bloody CSF were co-cultured in vitro with PBMCs. The content of sIL-2r in the culture medium, having been maintained at around 1.30 ng/ml during the first 3 d, had increased by Day 4. The content of sIL-2r peaked 7.53 ng/ml on Day 7 and then reduced gradually. Conclusions: (1) Basic HLA-DR expression is present in arachnoid cells. (2) After stimulation by bloody CSF, arachnoid cells have the potential to serve as antigen presenting cells (APCs) and the ability to activate T-lymphocytes, indicating that arachnoid cells are involved in the mechanism of coagulation-initiated inflammation in the subarachnoid space after SAH. 相似文献
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目的：探讨老年脑梗死患者血清和脑脊液（CSF）中C-反应蛋白（CRP）的水平及临床意义。方法：对20例老年脑梗死患者进行血清和CSF中CRP的检测，分析血清CRP与CSFCRP的相关性。结果：直线相关分析显示，血清CRP与CSFCRP水平呈正相关（r=0．9606，P=0．01）。结论：老年脑梗死患者CSF出现CRP的基础可能是血脑屏障受损。 相似文献
15.
The clinical diagnosis of sepsis is difficult, particularly in neonates. To devise a rapid and reliable method for identifing
bacteria in blood and cerebrospinal fluid (CSF), we developed a pair of primers according to the gene encoding 16 s rRNA,
found in all bacteria. DNA fragments from different bacterial species and from clinical samples were detected with polymerase
chain reaction (PCR), and with reverse hybridization using a universal bacterial probe, a gram-positive probe and a gram-negative
probe. Our results showed that a 371 bp DNA fragment was amplified from 20 different bacterial species. No signal was observed
when human DNA and viruses were used as templates. The sensitivity could be improved to 10−12 g. All 26 culture-positive clinical samples (22 blood samples and 4 CSF samples), were positive with PCR. The gram-negative
and gram-positive probe hybridized to clinical samples and to known bacterial controls, as predicted by Gram’s stain characteristics.
Our results suggest that the method of PCR and reverse hybridization is rapid, sensitive and specific in detecting bacterial
infections. This finding may be significant in the clinical diagnosis of sepsis in neonates.
Project (396457) supported by the Zhejiang Provincal Natural Science Foundation. 相似文献
16.
胸水腺苷脱氨酶检测对结核性胸膜炎的诊断意义 总被引：3，自引：0，他引：3
目的：探讨胸水的腺苷脱氨酶（ADA）测定值对结核性胸膜炎的诊断意义和应用价值。方法：对436例住院的胸腔积液患者分别行结核菌素纯蛋白衍生物（PPD）试验，同时应用酶速率法检测胸水标本中ADA的活性．比较二者的灵敏度及特异度。所有患者试验前均未行抗结核治疗。结果：ADA试验灵敏度（96．1％）高于PPD（90．2％）。二者差异有显著性（P〈0．05）。结论：胸腔积液ADA含量的检测对于早期诊断及治疗结核性胸膜炎具有积极意义。 相似文献
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The inherent spatial complexity of the human cerebral ventricular system, coupled with its deep position within the brain, poses a problem for conceptualizing its anatomy. Cadaveric dissection, while considered the gold standard of anatomical learning, may be inadequate for learning the anatomy of the cerebral ventricular system; even with intricate dissection, ventricular structures remain difficult to observe. Three-dimensional (3D) computer reconstruction of the ventricular system offers a solution to this problem. This study aims to create an accurate 3D computer reconstruction of the ventricular system with surrounding structures, including the brain and cerebellum, using commercially available 3D rendering software. Magnetic resonance imaging (MRI) scans of a male cadaver were segmented using both semiautomatic and manual tools. Segmentation involves separating voxels of different grayscale values to highlight specific neural structures. User controls enable adding or removing of structures, altering their opacity, and making cross-sectional slices through the model to highlight inner structures. Complex physiologic concepts, such as the flow of cerebrospinal fluid, are also shown using the 3D model of the ventricular system through a video animation. The model can be projected stereoscopically, to increase depth perception and to emphasize spatial relationships between anatomical structures. This model is suited for both self-directed learning and classroom teaching of the 3D anatomical structure and spatial orientation of the ventricles, their connections, and their relation to adjacent neural and skeletal structures. 相似文献
19.
Zhou-yan Feng Xiao-jing Zheng Jing Wang 《Journal of Zhejiang University. Science. B》2009,10(7):505-511
Objective: To directly examine the effects of carnosine on neuronal excitation and inhibition in rat hippocampus in vivo. Methods: Artificial cerebrospinal fluid with carnosine was directly administrated over the exposed rat hippocampus. The changes of neuron activity in the CA1 region of hippocampus were evaluated by orthodromically- and antidromically-evoked potentials, as well as paired-pulse stimulation paradigm. Results: In both orthodromic and antidromic response potentials, carnosine transformed population spikes (PSs) with single spike into epileptiform multiple spikes. In addition, similar to the effect of γ-aminobutyric acidA (GABAA) antagonist picrotoxin, carnosine decreased paired-pulse stimulating depression significantly.However, no significant change was observed in the spontaneous field potentials during the application of carnosine. Conclusion:The results indicate a disinhibition-induced excitation effect of carnosine on the CA1 pyramidal neurons. It provides important information against the application of carnosine as a potential anticonvulsant in clinical treatment. 相似文献