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1.
用不同浓度的芫荽、韭菜种子的浸提液处理黄瓜种子,通过对其种子萌发及幼苗生长的观察,结果表明:不同浓度的浸提液对植物的化感作用强度不同.  相似文献   

2.
目的:建立一种可靠、快速、便捷、经济的乙酰胆碱酯酶(acetylcholinesterase,AChE)抑制剂的筛选方法,为新药发现阶段AChE抑制剂的筛选提供经济可靠的微量筛选方法,然后对植物提取物样品进行筛选。方法:分别采用电鳗AChE、人源红细胞AChE、大鼠海马区、纹状体匀浆液中的AChE为酶源。以96孔板为载体,利用优化的Ellman方法,在生理pH值和温度的条件下,以他克林和石杉碱甲作为阳性对照,检测对不同酶源AChE的抑制作用,确定最佳酶反应条件(最佳底物浓度、反应时间、显色剂浓度),并对植物提取物样品的AChE抑制作用进行检测。结果:采用上述四种酶源的AChE进行AChE抑制剂微量筛选方法操作简便快速、可靠经济。其中应用电鳗AChE进行筛选显示灵敏度较高,他克林和石杉碱甲对电鳗AChE的抑制作用相当,而对人源红细胞AChE、大鼠海马区、纹状体匀浆酶源的抑制作用,石杉碱甲明显优于他克林,所筛选植物提取物对AChE均没有抑制作用。结论:本实验中所建立的筛选方法操作简便、快速、可靠经济,可用于纯化合物的筛选。  相似文献   

3.
两株草甘膦降解真菌的分离及其降解效能研究   总被引:7,自引:0,他引:7  
草甘膦是目前世界上广泛使用的一种有机磷除草剂,长期大量使用所造成的环境污染已不容忽视.微生物降解是生物修复草甘膦污染土壤的有效途径之一.从受草甘膦污染严重土壤中富集、筛选并分离到6株降解菌,包括2株细菌,4株真菌.选取降解效果较好的两株真菌HS-04和HS-05进行了初步研究.结果表明,HS-04和HS-05均能以草甘膦作为唯一的碳源和氮源生长.在基础培养基中,30℃、150 rpm条件下,6 d内对浓度为200 m g/L的草甘膦的降解率分别为85%和91%.两株真菌在草甘膦浓度为400~600 m g/L时生长较好.  相似文献   

4.
为研究马铃薯对枸杞种子萌发是否有影响,以宁夏枸杞为试验材料,用不同质量浓度的马铃薯块茎浸提液对枸杞种子进行处理,研究处理后枸杞种子的发芽率、发芽势、发芽指数和活力指数的变化.结果表明:马铃薯块茎浸提液对枸杞种子的发芽率、发芽势、发芽指数和活力指数都有不同程度的抑制作用.质量浓度为25、50、100 g/L的马铃薯块茎浸提液处理的种子与对照组发芽率无显著性差异,只有质量浓度为200 g/L处理的种子与对照组发芽率有显著性差异;质量浓度为25、50、100、200 g/L的马铃薯块茎浸提液处理的种子与对照组发芽势有极显著性差异,与对照组的发芽指数和活力指数都有显著性差异.  相似文献   

5.
草甘膦降解菌C-17的分离鉴定及生物学特性   总被引:1,自引:0,他引:1  
从福建三农集团污水处理池分离到一株降解草甘膦的菌株C—17,经形态与生理生化实验,C-17初步鉴定为胶红酵母属真菌。在草甘膦基础培养基上,C-17菌株对草甘膦的最高耐受浓度为6000mg/L,草甘膦浓度为3000mg/L时生长量最高。C-17菌株能以草甘膦为唯一氮、磷源,培养7d对草甘膦的降解率为83.6%,培养基中缺乏别的磷源,有利于促进C-17菌株对草甘膦的降解。  相似文献   

6.
本文采用亚磷酸二甲酯酸性水解法合成草甘膦.研究了不同盐酸、甲醇和脱酸真空度对草甘膦原粉收率的影响;初探了不同叔胺对草甘膦合成的催化活性.结果表明:盐酸纯度和脱酸真空度对草甘膦原粉收率影响明显;芳香叔胺无催化活性,烷基叔胺催化活性随烷基c原子数减少而增强.  相似文献   

7.
将新鲜的夹竹桃叶片洗净,叶片表面上的水分晾干,然后叶片表面杀菌处理并研碎,制成不同浓度的水提取液.以枯草芽孢杆菌和巨大芽孢杆菌为供试菌种,用贴片法对夹竹桃叶水提液的抑菌作用进行了研究.实验结果表明,夹竹桃水提液对以上两种供试菌种所产生的抑菌圈大小不同,不同浓度的夹竹桃叶水提液有不同程度的抑菌作用.随着其浓度的增大,抑菌作用增强.  相似文献   

8.
目的:利用小檗碱研究抗脂质堆积肝细胞模型药物的最佳实验条件.方法:将培养12h的HepG2细胞分为正常对照组(Con)、模型对照组(Model)和阳性对照药组(Ber).Con细胞仅用培养液培养,Model和Ber加入脂质堆积诱导液,后者再加不同浓度的小檗碱,分别观察不同的诱导时间对肝细胞脂质堆积的影响和对小檗碱治疗作用的影响.以油红0染色观察细胞内脂滴形成状况,并检测细胞内TG的含量.结果:1mM油酸钠、50nM胰岛素共同诱导48 h的实验条件可见肝细胞内有典型的脂滴形成,肝细胞内TG含量显著升高,差异有统计学意义(P〈0.01),终浓度10-5M小檗碱与模型对照组比较,能明显改善肝细胞脂质堆积,差异具有统计学意义((P〈0.001)可为对照阳性药,此条件比较适合样品的筛选.结论:确定了该细胞模型用于药物筛选的最佳实验条件.  相似文献   

9.
草甘膦对3种滩涂动物的急性毒性及安全评价   总被引:2,自引:0,他引:2  
采用静水法和半静水法检测不同浓度下除草剂草甘膦对缢蛏、可口革囊星虫和大弹涂鱼的急性毒性,并根据·化学农药环境安全评价试验准则·中农药的毒性分级,评价了草甘膦对三种滩涂生物的安全性.结果显示:草甘膦对于缢蛏、可口革囊星虫和大弹涂鱼的96 h LC50分别为233.60,1267.00,1068.00 mg·L-1,均属于低毒;安全浓度分别为23.36,126.70,106.80 mg·L-1,毒性顺序为缢蛏>大弹涂鱼>可口革囊星虫.  相似文献   

10.
以市场常见凤尾菇为材料,在Pachlewski中培养2周后,菌丝体置于在2mg·kg-1Hg2 浓度的吸收液中,分别加入0.5mM的EDTA、CaH2PO4,并设置吸收液不同pH(3.8,8.5)。经一定的时间间隔(5、10、20、40、60、120、180min),连续测定吸收液中汞浓度的变化情况,研究凤尾菇菌丝体的汞吸收特性。结果表明,在加入CaH2PO4、EDTA以及不同环境pH下,吸收液中总汞浓度的呈指数下降规律,符合主动吸收的规律。180分钟时,经不同大小的菌丝体吸收后,相同处理吸收液中总汞的残留量非常一致,但不同处理有显著差异;不同单因子条件下汞残留浓度的差异,反映出处理因子对凤尾菇菌丝体汞吸收特性的影响。  相似文献   

11.
胆碱酯酶与有机磷农药中毒   总被引:1,自引:0,他引:1  
有机磷农药中毒的机制,主要是有机磷农药与乙酰胆碱酯酶(AChE)结合成中毒酶,AChE失去催化水解乙酰胆碱(Ach)的活性;导致胆碱能神经突触间隙ACh大量蓄积.大量ACh对受体的作用大大增强和延长,导致中枢和周围胆碱能神经系统功能紊乱;此外,农药也可直接作用于胆碱能受体.中毒酶形成后有3种去向.  相似文献   

12.
文章研究了黄粉虫乙酰胆碱酯酶(AChE)的体躯分布和纯化方法技术。结果表明:黄粉虫幼虫和成虫体内乙酰胆碱酯酶活性均集中在头部和胸部,腹部的乙酰胆碱酯酶活性显著低于头部和胸部。成虫体内乙酰胆碱酯酶活性明显高于幼虫。通过Sepharose4B和SephadexG-200纯化后,AChE的最高纯化倍数是3.86和18.84倍。  相似文献   

13.
Achievement emotions are critical because of their impact on success and failure in important domains such as learning. These emotions may be modified via emotion regulation (ER). The dominant process model of ER (PMER) proposed by J. Gross, however, provides a domain-general account of ER strategies and has not had substantial contact with theories of achievement emotions such as R. Pekrun’s control-value theory (CVT) and the academic achievement literature. Moreover, ER has not been a focal point of major theories related to achievement emotions, such as CVT. We propose an integrated model of ER in achievement situations (ERAS) that integrates propositions about the generation of emotions from CVT with propositions about how emotions are regulated and types of ER strategies from PMER. The ERAS model also offers new propositions regarding how different achievement situations, object foci, and time frames, as well as discrete emotions with different appraisal patterns, impact ER strategies.  相似文献   

14.
力竭运动状态下线粒体结构和功能发生改变,使线粒体发生空泡样变,致使ATP生成减少,影响了运动能力。笔者对力竭运动对线粒体结构,功能的影响进行简要的综述.  相似文献   

15.
本实验用视和组化法对26例胃癌患者的雌、孕激素受体进行了检测。结果表明:低分化腺癌ER阳性病例中男性多于女性,而管状腺癌则女性略多于男性。在不同类型的胃性肿瘤中,ER阳性病例以管状腺癌的发生率最高,低分化腺癌次之。淋巴细胞浸润情况,低分化腺癌表现为ER阳性病例少于阴性病例,管状腺察则阳阴性病例相差不明显.核色质的粗细、核分裂象等情况,在不组织学类型中,其阳阴性差別不明显。  相似文献   

16.
This study examined the contribution of attachment security and emotion regulation (ER) to the explanation of social information processing (SIP) in middle childhood boys with learning disabilities (LD) and without LD matched on age and grade level. Children analyzed four social vignettes using Dodge's SIP model and completed the Kerns security scale and the children's self-control scale. Study results demonstrated major difficulties in SIP, lower attachment security, and less ER in children with LD compared to children without LD. Attachment as well as the interaction between attachment and ER emerged as important contributors to most SIP steps, suggesting that children with higher security who also have better ER skills will have better SIP capabilities along the different steps, beyond group inclusion. Results were discussed in terms of practical and clinical implications regarding the importance of mother-child attachment and ER skills for social cognitive capabilities in children with LD.  相似文献   

17.
This study examined emotion regulation (ER) as a mediator in the relation between early behavioral inhibition (BI) and later social competence (= 257), and whether this mediation varied depending on BI levels. Maternal report and observational measures were used to assess BI (ages 2 and 3). Children's ER strategies (age 5) and social competence with an unfamiliar peer (age 7) were measured using observational measures. Results showed that BI predicted less engaged ER strategies during a disappointment task, and engaged ER predicted higher social competence. Engaged ER mediated the effect of BI on social competence, but only for highly inhibited children. Findings elucidate developmental trajectories of risk and resilience, and suggest targeting regulatory strategies in early prevention efforts with highly inhibited children.  相似文献   

18.
Longitudinal relations among ego‐resiliency (ER), effortful control (EC), and observed intrusive parenting were examined at 18, 30, and 42 months of age (Ns = 256, 230, and 210) using structural equation modeling. Intrusive parenting at 18 and 30 months negatively predicted EC a year later, over and above earlier levels. EC at 30 months mediated the negative relation between 18‐month intrusive parenting and ER at 42 months when controlling for stability of the variables. ER did not predict EC. The findings suggest that intrusive parenting may have a negative effect on children's ego‐resiliency through its effects on children's abilities to regulate attention and behavior.  相似文献   

19.
牡丹绒毡层的超微结构   总被引:1,自引:0,他引:1  
用透射电镜观察了牡丹(Paeonia suffruticosa Andr.)花粉发育过程中绒毡层的超微结构。在花粉母细胞减数分裂形成四分体阶段,绒毡层的内质网逐渐增多,小液泡、质体、线粒体等细胞器也逐渐增多。在四分体时期后,内质网和小液泡继续增多,开始出现脂肪滴,其他细胞器结构简化、数量减少,以至完全退化消失。在小孢子晚期,不同花药的绒毡层分化为分泌型和变形型两种类型。分泌型绒毡层的内质网较变形绒毡层的发达,并不断向花粉粒分泌脂肪滴和小泡,变形绒毡层解体后原生质围绕于花粉的周围,供其发育之需。在二胞晚期,两种类型的绒毡层都完全消失。  相似文献   

20.
Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides.  相似文献   

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