Pheromone synthesis in a biomicroreactor coated with anti-adsorption polyelectrolyte multilayer     

Dimov N  Muñoz L  Carot-Sans G  Verhoeven ML  Bula WP  Kocer G  Guerrero A  Gardeniers HJ 《Biomicrofluidics》2011,5(3):34102-3410212

To prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His(6)-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS).  相似文献   

Disposable parallel poly(dimethylsiloxane) microbioreactor with integrated readout grid for germination screening of Aspergillus ochraceus     

Demming S  Sommer B  Llobera A  Rasch D  Krull R  Büttgenbach S 《Biomicrofluidics》2011,5(1):14104

In this work a disposable, parallel microbioreactor (MBR) suitable for screening in batch or continuous mode is presented. The reactor consists of five parallel microchambers made of poly(dimethylsiloxane) bonded to a glass substrate. A grid structure is engraved on each chamber, allowing subsequent morphology imaging. Measurements are recorded over the entire cultivation period with constant parameters, namely, position and focus in the z-axis. The microdevice may be used for either parallel, uni- or multiparametric screening, and overcomes the drawback of gridless microwell plates which require expensive equipment such as an inverted microscope with an automatic stage. To validate the scalability from laboratory scale to microscale, and thus the cultivation protocol in the MBR, the germination of fungal spores (A. ochraceus) is evaluated for two different key magnitudes (pH and temperature) and compared to the results obtained from conventional laboratory scale systems (flasks and agar plates). Information on germination capacity with regard to interspecies' variability allows for optimization of industrial processes as optimal pH and temperature matched to the mesoscopic cultivation systems. The germination conditions therefore remain unaffected inside the MBR, while providing the following advantages: (i) dramatic reduction of medium consumption, (ii) submerged cultivation with constant oxygen supply, (iii) assured low cost and disposability, and (iv) possibility of a continuous cultivation mode.  相似文献   

A comparison of anthropometric and training characteristics of Ironman triathletes and Triple Iron ultra-triathletes     

Knechtle B  Knechtle P  Rüst CA  Rosemann T 《Journal of sports sciences》2011,29(13):1373-1380

We examined differences in anthropometry and training between 64 Triple Iron ultra-triathletes competing over 11.4 km swimming, 540 km cycling, and 126.6 km running, and 71 Ironman triathletes competing over 3.8 km swimming, 180 km cycling, and 42.2 km running. The association of anthropometry and training with race time was investigated using multiple linear regression analysis. The Triple Iron ultra-triathletes were smaller (P < 0.05), had shorter limbs (P < 0.05), a higher body mass index (P < 0.05), and larger limb circumferences (P < 0.01) than the Ironman triathletes. The Triple Iron ultra-triathletes trained for more hours (P < 0.01) and covered more kilometres (P < 0.01), but speed in running during training was slower compared with the Ironman triathletes (P < 0.01). For Triple Iron ultra-triathletes, percent body fat (P = 0.022), training volume per week (P < 0.0001), and weekly kilometres in both cycling (P < 0.0001) and running (P < 0.0001) were related to race time. For Ironman triathletes, percent body fat (P < 0.0001), circumference of upper arm (P = 0.006), and speed in cycling training (P = 0.012) were associated with total race time. We conclude that both Triple Iron ultra-triathletes and Ironman triathletes appeared to profit from low body fat. Triple Iron ultra-triathletes relied more on training volume in cycling and running, whereas speed in cycling training was related to race time in Ironman triathletes.  相似文献   

BISp Informationen     

Martin-Peter Büch  Heiner Schumann  Georg Anders  Dietrich Hartmann 《Sportwissenschaft》2011,41(4):350-351

BISp-Informationen

BISp Informationen  相似文献   

Informationen des Bundesinstituts für Sportwissenschaft (BISp)     

Martin-Peter Büch  Heiner Schumann  Georg Anders  Dietrich Hartmann 《Sportwissenschaft》2011,41(3):255-256

BISp-Informationen

Informationen des Bundesinstituts für Sportwissenschaft (BISp)  相似文献   

Sportwissenschaftliches aus Japan     

Prof. Dr. Michael Krüger 《Sportwissenschaft》2011,41(1):72-74

  相似文献   

Vapor-liquid equilibrium simulation of binary and ternary mixtures of CH4, C2H4 and iso-C4H10     

张吕鸿  张晶  孙永利  闫娇  刘玉花  刘萌萌 《天津大学学报(英文版)》2014,20(2):79-85

The vapor-liquid equilibrium（VLE） properties for the binary and ternary mixtures of CH4,C2H4 and isoC4H10 are of great importance in the recovery of ethylene from mixture containing CH4 and C2H4 with iso-C4H10 as solvent.Hence,Gibbs ensemble Monte Carlo（GEMC） simulations were used to estimate vapor-liquid equilibrium for the binary and ternary mixtures of CH4,C2H4 and iso-C4H10 with the united atom potential NERD model.The selected simulation conditions are based on the experiment in the literature.The results of this work were shown to be in satisfactory agreement with available experimental data and predictions of Peng-Robinson equation of state.The structure of simulated liquid phase is also characterized by radial distribution function（RDF）,which contributes to further understanding of the VLE curve of these systems.RDF is not sensitive to the pressure and temperature range.With the increase of pressure or the decrease of temperature,the molecules tend to gather together.  相似文献   

基于专利数据的技术融合研究综述     

吕璐成  赵亚娟 《图书情报工作》2021,65(6):138-148

[目的/意义] 基于专利数据开展技术融合研究是技术融合研究的主要方式和热点方向,面向为技术融合后续研究工作开展提供参考和启发的目标,对国内外基于专利数据的技术融合研究现状进行较为全面的综述。[方法/过程] 将已有研究按研究内容划分为技术融合测度及预测方法研究、特定领域/多领域间/全领域的技术融合态势测度及趋势预测、技术融合的测度指标研究、影响技术融合的特征因素研究、基于技术融合视角进行技术机会发现以及技术融合与创新的关系研究六类,并对各类别取得的研究成果进行评述。[结果/结论] 基于专利数据开展技术融合研究已经取得一定的成果,但是仍旧存在测度依据不合理、预测方法缺乏验证、全领域研究关注度低的问题,这些问题可通过引入语义关系优化技术融合网络、采用图神经网络技术改进技术融合预测方法、完善全领域技术融合测度和预测方法来解决。  相似文献   

Evaluation of the level of problem solving skills of Turkish higher education graduates in technology-rich environments     

Açıkgöz  Ömer  Aslan  Aydın  Günay  Aslı 《Educational technology research and development : ETR & D》2022,70(5):1893-1910

Educational technology research and development - This study aims to evaluate the level of Turkish higher education graduates’ problem-solving skills (PSSs) in technology-rich environments...  相似文献   

A meta-analysis of the impact of mobile learning on mathematics achievement     

Güler  Mustafa  Bütüner  Suphi Önder  Danişman  Şahin  Gürsoy  Kadir 《Education and Information Technologies》2022,27(2):1725-1745

Education and Information Technologies - The technological developments of the twenty-first century have enabled the emergence of alternative teaching-learning models and instructional tools. One...  相似文献