从高职院校学生的阅读倾向谈高校图书馆的导读工作——以广东外语艺术职业学院为例     

杜穗 《江西图书馆学刊》2009,39(1):61-64

以统计分析广东外语艺术职业学院学生的阅读倾向为基础,探讨了图书馆导读工作的必要性和重要性．并提出了做好高校图书馆导读工作的建议。  相似文献   

高校图书馆如何为地方科技创新服务   总被引：1，自引：0，他引：1  

盛穗  姚建文 《高校图书馆工作》2009,29(2):77-78

为地方科技创新服务,高校图书馆应发挥自身的优势,拓宽服务领域,向地方提供及时、有效、可靠的信息服务。参考文献3。  相似文献   

清王芑孙手批总集、别集三种述略     

眭骏 《图书馆杂志》2009,28(3)

王芑孙是清乾嘉间知名学者,以辞章之学享誉海内.本文择所寓目之王氏手批总集、别集三种,作一番介绍.  相似文献   

合作与引领发展:"长三角"高等教育行动   总被引：1，自引：0，他引：1  

眭依凡 《中国高教研究》2010,(6)

长三角地区具备经济社会和高等教育资源条件优势,其高等教育的发展,必须借鉴欧盟博洛尼亚进程的成功经验,建立"长三角高等教育合作区",以根本打破高等教育体制的区域壁垒,有效整合高等教育资源,加速高等教育的国际化和现代化,引领我国高等教育的改革和发展.关于当前长三角高等教育势在必行的合作行动,选择路径有:推行长三角高校联考招生制度以推动素质教育;形成高等教育优质资源共享机制,提升精英教育和科技创新的水平; 出台高等教育政策,缩小长三角地区高等教育的均衡发展差距;尽快化解高校债务风险,建立无债务长三角高等教育区.  相似文献   

和谐世界与当代国际共运之关系     

眭传厚  支娜娜  疏唐泽 《台州学院学报》2009,31(1):5-10

和谐世界理论的内涵不仅有其字面的显义,而且有其字里行间的深层寓意,虽然对国际共运未予正面论述,而隐含的意思却与当代国际共运息息相关;国际共运尽管处在低潮,但它的存在却是客观事实,和谐世界的主张对当代的国际共运将产生重大影响;和谐世界的主张不同于“和平长入社会主义”,也不排斥暴力革命论,它是对马克思主义和平变革论的继承和发展。  相似文献   

A maximum power point tracker for photovoltaic energy systems based on fuzzy neural networks   总被引：1，自引：0，他引：1  

Chun-hua Li  Xin-jian Zhu  Guang-yi Cao  Wan-qi Hu  Sheng Sui  Ming-ruo Hu 《浙江大学学报(A卷英文版)》2009,10(2):263-270

To extract the maximum power from a photovoltaic （PV） energy system, the real-time maximum power point （MPP） of the PV array must be tracked closely. The non-linear and time-variant characteristics of the PV array and the non-linear and non-minimum phase characteristics of a boost converter make it difficult to track the MPP for traditional control strategies. We propose a fuzzy neural network controller （FNNC）, which combines the reasoning capability of fuzzy logical systems and the learning capability of neural networks, to track the MPP. With a derived learning algorithm, the parameters of the FNNC are updated adaptively. A gradient estimator based on a radial basis function neural network is developed to provide the reference information to the FNNC. Simulation results show that the proposed control algorithm provides much better tracking performance compared with the filzzy logic control algorithm.  相似文献   

An integrated microfluidic device for rapid serodiagnosis of amebiasis     

Wang Zhao  Li Zhang  Wenwen Jing  Sixiu Liu  Hiroshi Tachibana  Xunjia Cheng  Guodong Sui 《Biomicrofluidics》2013,7(1)

A microfluidic device was successfully fabricated for the rapid serodiagnosis of amebiasis. A micro bead-based immunoassay was fabricated within integrated microfluidic chip to detect the antibody to Entamoeba histolytica in serum samples. In this assay, a recombinant fragment of C terminus of intermediate subunit of galactose and N-acetyl-_D-galactosamine-inhibitable lectin of Entamoeba histolytica (C-Igl, aa 603-1088) has been utilized instead of the crude antigen. This device was validated with serum samples from patients with amebiasis and showed great sensitivity. The serodiagnosis can be completed within 20 min with 2 μl sample consumption. The device can be applied for the rapid and cheap diagnosis of other infectious disease, especially for the developing countries with very limited medical facilities.Entamoeba histolytica is the causative agent of amebiasis and is globally considered a leading parasitic cause of human mortality.¹ It has been estimated that 50 × 10⁶ people develop invasive disease such as amebic dysentery and amebic liver abscess, resulting in 100 000 deaths per annum.^{2, 3} High sensitive diagnosis method for early stage amebiasis is quite critical to prevent and cure this disease. To date, various serological tests have been used for the immune diagnosis of amebiasis, such as the indirect fluorescent antibody test (IFA) and enzyme-linked immunosorbent assay (ELISA).We have recently identified a 150-kDa surface antigen of E. histolytica as an intermediate subunit (Igl) of galactose and N-acetyl-_D-galactosamine-inhibitable lectin.^{4, 5} In particular, it has been shown that the C-terminus of Igl (C-Igl, aa 603-1088) was an especially useful antigen for the serodiagnosis of amebiasis. ELISA using C-Igl is more specific than the traditional ELISA using crude antigen.⁶ However, the ELISA process usually takes several hours, which is still labor-intensive and requires experienced operators to perform. More economic and convenient filed diagnosis methods are still in need, especially for the developing countries with limited medical facilities.Among all the bioanalytical techniques, microfluidics has been attracting more and more attention because of its low reagent/power consumption, the rapid analysis speed as well as easy automation.^{7, 8, 9, 10, 11} Especially with the development of the fabrication technique, microfluidics chip can include valves, mixers, pumps, heating devices, and even micro sensors, so many traditional bioanalytical methods can be performed in the microfluidics. Qualitative and quantitative immune analysis on the microfluidic chip was successfully proved by plenty of research with improved sensitivity, shorten reaction time, and less sample consumption.^{8, 10, 11, 12, 13, 14, 15, 16, 17} Moreover, with the intervention of other physical, chemical, biology, and electronic technology, microfluidic technique has been successfully utilized in protein crystallization, protein and gene analysis, cell capture and culturing and analysis as well as in the rapid and quantitative detection of microbes.^{13, 14, 15, 16, 17, 18, 19, 20}Herein, we report a new integrated microfluidic device, which is capable of rapid serodiagnosis of amebiasis with little sample consumption. The microfluidic device was fabricated from polydimethysiloxane (PDMS) following standard soft lithography.^{21, 22} The device was composed of two layers (shown in Figure ​Figure1)1) including upper fluidic layer (in green and blue) and bottom control layer (in red).Open in a separate windowFigure 1Structure illustration of microfluidic chip.To create the fluidic layer and the control layer, two different molds with different patterns have fabricated by photolithographic processes. The mold to create the fluidic channels was made by positive photoresist (AZ-50 XT), while the control pneumatic mold was made by negative photoresist (SU8 2025). For the chip fabrication, the fluidic layer is made from PDMS (RTV 615 A: B in ratio 5:1), and the pattern was transferred from the respective mold. The control layer is made from PDMS (RTV 615 A:B in ratio 20:1). The two layers were assembled and bonded together accurately, and there is elastic PDMS membrane about 30 μm thick between the fluidic layer channels and control layer.^{21, 22} The elastic membrane at the intersection can deform to block the fluid inside the fluidic channels, functioning as valves under the pressures introduced though control channels. There are two types of channels in fluidic layer, the rectangular profiled (in green, 200 μm wide, 35 μm thick) channel and round profiled channels (in blue, 200 μm wide, 25 μm center height). Because of the position of the valves on the fluidic channels, two types of valves (Figure ​(Figure2a)2a) were built, working as a standard valve and a sieve valve. The standard valves (on blue fluidic channels) can totally block the fluid because of the round profile of fluidic channel; the sieve valve can only half close because of the rectangular profile. The sieve valve can be used to trap the microspheres (beads) filled inside the green fluidic channels, while letting the fluid pass through. By this sieve valve, a micro column (in green) is constructed, where the entire ELISA reaction happens. The micrograph of the fabricated micro device is shown in Figure ​Figure2b.2b. The channels were filled with food dyes in different colors to show the relative positions of the channels. The pressures though different control channels are individually controlled by solenoid valves, connected to a computer through relay board. By programming the status (on/off) of various valves at different time periods, all the microfluidic chip operation can be digitally controlled by the computer in manual, semi-automatic, or automatic manner.Open in a separate windowFigure 2(a) Structure illustration of micro column, standard valve and sieve valve; (b) photograph of the microfluidic chip.To validate this device, 12 patient serum samples were collected. Sera from 9 patients (Nos. 1–9) with an amebic liver abscess or amebic colitis were used as symptomatic cases. The diagnosis of these patients was based on their clinical symptoms, ultrasound examination (liver abscess) and endoscopic or microscopic examination (colitis). We also identified the clinical samples using PCR amplification of rRNA genes.²⁴ As negative control, sera obtained from 3 healthy individuals with no known history of amebiasis were mixed into pool sera. The serum was positive for E. histolytica with a titer of 1:64 (borderline positive), as determined by an indirect fluorescent-antibody (IFA) test.^{23, 24} In our previously study, the sensitivity and specificity of the recombinant C-Igl in the ELISA were 97% and 99%.^{6, 25} In the current study, the serodiagnosis of amebiasis was also examined by ELISA using C-Igl.²⁶ The cut-off for a positive result was defined as an ELISA value > 3 SD above the mean for healthy negative controls²⁷ (shown in Figure ​Figure3).3). The seropositivity to C-Igl was 100% in patients with amebiasis.Open in a separate windowFigure 3ELISA reactivity of sera from patients against C-Igl. ELISA plate was coated with 100 ng per well of C-Igl. Serum samples from patients and healthy controls were used at 1:400 dilutions. The dashed line indicates the cut-off value. Data are representative of results from three independent experiments.In the diagnosis process with microfluidic chip, the 4 micro immuno-columns filled with C-Igl-coated microspheres were the key components of the device. The C-Igl was prepared in E. coli as inclusion bodies. After expression, the recombinant protein was purified and analyzed by SDS-PAGE. The apparent molecular mass was 85 kDa.²⁶The immune-reaction mechanism is illustrated in Figure ​Figure4.4. The anti-His monocolonal antibody was immobilized onto the microspheres (beads, 9 μm diameter) coated with protein A. The C-Igl was then immobilized onto the beads through the binding between the His tag and C-Igl. For the diagnosis, the microspheres immobilized with C-Igl and blocked by 5% BSA were preloaded into the columns for the rapid analysis of the patient serum samples. Generally, serum samples which were diluted 100 times were first loaded into the reaction column and incubated at room temperature for 5 min. After being washed by PBS buffer, FITC-conjugated goat anti-human polyclonal antibody was added into the column for 4 min incubation. The fluorescence image can be collected by the fluorescence microscope after the micro column was washed with PBS buffer. From loading diluted serum samples into column to collecting fluorescence images, the total time to complete the immunoassay is less than 10 min. The final fluorescence results were analyzed by Image Pro Plus 6.0.Open in a separate windowFigure 4Schematic representation of the ELISA in the chip.Different reaction conditions have been investigated to find the optimized ones. For each patient, 2 μl sample is enough for the analysis. The designed microfluidic chip with 4 micro columns is capable for 4 parallel analyses at the same time. More micro columns can be integrated into the device if more parallel tests are needed.Different incubating time for the diagnosis has also been investigated and no significant difference has been found for various time periods. It is enough to incubate the chip for only 5 min. The total diagnosis time for one sample is less than 10 min. The detection result appeared as the fluorescence intensity of the reaction column. As shown in Figure ​Figure5,5, the negative sample showed relatively low fluorescence intensity, because little FITC-conjugated goat anti-human polyclonal antibody could attach to the surface of microspheres; on the contrast, the positive sample showed much brighter fluorescence. The fluorescence intensity can be transferred to digital data (Table