Sequential Serum Cytokine Levels of TNF-Alpha,IL-4 and IL-12 are Associated with Prognosis in Plasmodium falciparum Malaria     

P. C. Mohapatra  Anshuman Sarangi  Ashok Kumar Sarangi  R. K. Dalai  Debashis Sahoo 《Indian journal of clinical biochemistry : IJCB》2014,29(3):321-326

We investigated the prognostic role of TNF-alpha, IL-4 and IL-12 in a clinically well defined group of Plasmodium falciparum infected patients (n = 32) sequentially from Day 0 to Day 10 with a 2 day interval along with a control group of 16 healthy volunteers of same range of age and sex. Infection with malaria is often fatal because mitochondria are unable to generate enough ATP to maintain normal cellular function. ATP deficiency arises in malaria due to an inability of mitochondria through the effects of inflammatory cytokines on their function, to utilize available oxygen. In our study TNF-alpha and IL-12 levels were significantly elevated but IL-4 level showed persistent decline in Day 0, but subsequent measurement in Day 2, 4, 6, 8 and 10 showed persistent decline in levels of TNF-alpha and IL-12, an elevation in IL-4 levels which were associated with disease prognosis of the infected patients. These results again provide evidence that cytokines are very much a dominant partner in malaria pathogenesis with a specific prognostic role.  相似文献   

The Enunciation of the Subject: Sharing Jean-Luc Nancy’s Singular Plural in the Classroom     

Ashok Collins 《Educational Philosophy and Theory》2013,45(8):774-785

This article seeks to explore the implications of Jean-Luc Nancy’s reading of the subject for educational philosophy by connecting his re-interpretation of Descartes to his later thinking on what he names the ontological singular plural. Nancy’s re-imagining of the Cogito coalesces around the figure of the mouth (la bouche) through which the subject enunciates itself within the world. Reading this extension of the ego through the mouth as an enunciation of ontological singular plurality exposes a speaking subject that communicates via a sharing of its own being with other singular subjects. The article concludes that Nancy’s singular plural holds potential for affirming a substance-less subjectivity that can nevertheless serve as a locus of meaning in the classroom.  相似文献   

Does LDL-C Estimation Using Anandaraja’s Formula Give a Better Agreement with Direct LDL-C Estimation than the Friedewald’s Formula?     

Shalini Gupta  Minni Verma  Kamaljit Singh 《Indian journal of clinical biochemistry : IJCB》2012,27(2):127-133

Estimation of low density lipoprotein cholesterol (LDL-C) is crucial in management of coronary artery disease patients. Though a number of homogenous assays are available for estimation of LDL-C, use of calculated LDL-C by Friedewald’s formula (FF) is common in Indian laboratories for logistic reasons. Recently Anandaraja and colleagues have derived a new formula for calculating LDL-C. This formula needs to be evaluated before it is extensively applied in diagnosis. We measured LDL-C by homogenous method (D-LDL-C) in 515 fasting samples. Friedewald’s and Anandaraja’s formulas were used for calculation of LDL-C (F-LDL-C and A-LDL-C, respectively). The mean LDL-C levels were 123.3 ± 53.2, 112.4 ± 50.2 and 109.2 ± 49.8 mg/dl for D-LDL-C, F-LDL-C and A-LDL-C, respectively. There was a statistically significant difference between the results (P > 0.001) obtained by calculation formulas compared to the measured LDL-C. There was underestimation of LDL-C by 10.8 and 14 mg/dl by Friedewald’s and Anandaraja’s formulas respectively. The Pearson’s correlation between F-LDL-C and D-LDL-C was 0.931 and that between A-LDL-C and D-LDL-C was 0.930. Bland–Altman graphs showed a definite agreement between mean and differences of the calculation formulas and direct LDL-C with 95% of values lying with in ±2 SD limits. The mean percentage difference (calculated as {(Calculated LDL-C)-(D-LDL-C)}/D-LDL-C × 100) for F-LDL-C was maximum (−11.6%) at HDL-C ≥ 60 mg/dl and TG levels of 200–300 mg/dl (−10.4%) compared to D-LDL-C. A-LDL-C results gave highest mean percentage difference at total cholesterol concentrations <100 mg/dl (−37.3%) and HDL-C < 40 mg/dl (−17.1%), respectively. The results of our study showed that FF is better in agreement with D-LDL-C than Anandaraja’s formula for estimation of LDL-C by calculation though both lead to its underestimation.  相似文献   

Conservatism and personality factors in a sample of adolescents     

Gajendra K. Verma 《Contemporary educational psychology》1978,3(1):51-56

Relationships between Cattell High School Personality Questionaire factors and the adolescent version of the Wilson-Patterson Conservatism Scale were examined in a sample of 492 teenagers. Conservatism was significantly related to a number of personality traits in both boys and girls. Results suggest the existence of a “conservative personality.”  相似文献   

Study of adenosine deaminase activity in pulmonary tuberculosis and other common respiratory diseases     

Meena Verma  Sanjeev Narang  Ashish Moonat  Akshra Verma 《Indian journal of clinical biochemistry : IJCB》2004,19(1):129-131

Tuberculosis has, in a short span of time, become a major health problem in the third world or developing countries like India. In view of this, a retrospective study was conducted to study Adenosine deaminase activity in serum and pleural fluid in patients affected with Pulmonary Tuberculosis and other common non-tubercular chronic respiratory diseases. The study was carried out on 100 patients suffering from various pulmonary disorders, between January 2002 and August 2002. Thirty-five normal healthy individuals were included as control subjects. ADA estimation was done by sensitive colorimetric method. The study revealed that the serum ADA activity was higher in patients of tuberculous pulmonary and pleural diseases and non-tuberculous pulmonary diseases than in control subjects. The mean serum ADA activity in the patients’ group was 35.5±6.93 u/l as compared to 16.20±2.85 u/l in control group, showing a highly significant (P≪0.001) difference. ADA activity was highest in tuberculous pleuropulmonary diseases. The pleural fluid ADA activity was higher in pyogenic pleural effusion than in tuberculous pleural effusion.  相似文献   

Changes in biochemical constituents and induction of early sprouting by triadimefon treatment in white yam （Dioscorea rotundata Poir.） tubers during storage   总被引：1，自引：0，他引：1  

Jaleel CA  Gopi R  Manivannan P  Kishorekumar A  Gomathinayagam M  Panneerselvam R 《Journal of Zhejiang University. Science. B》2007,8(4):283-288

The ability of triadimefon(TDM),a triazolic fungicide,to alter the biochemical constituents and thereby minimizing the days required for sprouting in white yam(Dioscorea rotundata Poir.) tubers during storage under(30±2) °C in the dark,was studied.TDM at 20 mg/L was given to tubers by dipping the tubers in treatment solution containing 20 mg/L TDM on 10,25 and 40 d after storage(DAS).Starch,sugars,protein,amino acid contents as well as protease and α-amylase activities were estimated on 15,30 and 45 DAS from two physiological regions viz.,apical and basal regions of the tubers.In normal conditions(control) sprouting occurred on 70 to 80 DAS.The starch content decreased,while protein,amino acid,sugar contents and protease and α-amylase activities were increased due to TDM treatment and led to early sprouting.  相似文献   

Serum Lactate Dehydrogenase in Non-Hodgkin’s Lymphoma: A Prognostic Indicator     

Charu Yadav  Afzal Ahmad  Benedicta D’Souza  Ashish Agarwal  M Nandini  K Ashok Prabhu  Vivian D’Souza 《Indian journal of clinical biochemistry : IJCB》2016,31(2):240-242

Non-Hodgkin’s lymphoma constitutes a group of disorders originating from the malignant transformation of lymphocytes and involving either the lymph nodes or extranodal sites. NHL commonly presents in the sixth to seventh decade of life with a male preponderance (50–75 %). Recent studies have shown importance of serum LDH in prognosis of NHL. Authors report a case of a 63 year old male presenting with complaints of fever and backache for past 4 months. General and systemic examination revealed bilateral axillary lymphadenopathy and splenomegaly respectively. Serum LDH level was highly elevated (3441 U/l). Excisional axillary and bone marrow biopsy were done before oncology referral. Complete workup revealed diffuse Non-Hodgkin’s lymphoma with bone marrow infiltration. Patient died because of acute renal failure due to NHL and DM 2 (Type 2 diabetes mellitus).  相似文献   

Hypolipidemic Activity of Cassia tora Seeds in Hyperlipidemic Rats     

Vishnu Kumar Awasthi  Farzana Mahdi  Ramesh Chander  Ashok Kumar Khanna  Jitendra Kumar Saxena  Ranjana Singh  Abbas Ali Mahdi  Raj Kumar Singh 《Indian journal of clinical biochemistry : IJCB》2015,30(1):78-83

The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae) seeds extract have been studied in two models of hyperlipidemia in rats. In an acute model, hyperlipidemia was induced by injecting a single dose of Triton WR-1339 (400 mg/kg, b.w.) intraperitonially in rats. Feeding with C. tora seed extract at the dose of 500 mg/kg, b.w. exerted significant lipid lowering effect as assessed by the reversal of plasma levels of total cholesterol, phospholipids, triglyceride and reactivation of post heparin lipolytic activity. In the chronic model, hyperlipidemia was induced by feeding with cholesterol rich-HFD in rats. The treatment with seeds extract of C. tora (500 mg/kg, b.w.) simultaneously for 15 days also caused lowering of lipid levels in plasma and liver following reactivation of plasma post heparin lipolytic activity and hepatic lipoprotein lipase activity in animals. The hypolipidemic activity of C. tora seeds was compared with a standard drug guggulipid (200 mg/kg, b.w.) in both models.  相似文献   

Comparing age-wise reference intervals for serum creatinine concentration in a “Reality check” of the recommended cut-off     

Mascha Verma  Rashmi Khadapkar  Priyadarshi Soumyaranjan Sahu  Bibhu Ranjan Das 《Indian journal of clinical biochemistry : IJCB》2006,21(2):90-94

An increase in the communication within the healthcare services, both nationally and internationally, has strengthened the need for harmonization of measurements and reference intervals in laboratory medicine. In the present report, the calculated reference interval for serum creatinine (sCr) levels of healthy normal individuals (n=1121) in different sex and age groups are compared with the established interval. The calculated reference interval for sCr level was 0.4–1.3 mg/dL and 0.6 to 1.3 mg/dL in the age groups of 21–40 and 41–60 years respectively. The difference between the mean sCr values in total males and total females (age range 21–60 years) was statistically significant (p<0.0001); When male and female subjects were analyzed age-group wise, the data showed a significant difference in mean sCr values (p<0.0001) in three age groups (21–30, 31–40 and 41–50 years) however, in older age group (51–60 years), the difference was non-significant (p=0.07). The reference ranges were 0.7–1.3 and 0.4–1.0 mg/dL for males and females respectively where the lower limit was 0.1–0.2 units less than that of standard limits. An increase in the mean value of sCr was observed particularly in females with an increase in age. Hence it is of interest to validate an age specific reference ranges for sCr in our population.  相似文献   

Antioxidants: Basis concepts in relation to the eye     

Pradeep Venkatesh  Garg Satpal  Lalit Verma  Tewari Hem Kumar  Sheena Garg 《Indian journal of clinical biochemistry : IJCB》2001,16(1):9-14

Antioxidants are a small group of substances that protect living cells from the destructive consequences of powerful oxidizing intermediates that can be formed from oxygen. Situations in which pro-oxidant mechanisms within the body are more active than the antioxidant mechanisms (oxidative stress) predispose and contribute to the pathogenesis of several ailments in various organs of the body. In the eye, pro-oxidant factors have been blamed for the causation of diseases such as age related macular degeneration and senile cataract. The role of pro-oxidants in the genesis of certain diseases is well established however, the effectivity of antioxidants provided to the body by dietary supplementation is inconclusive. In this article we provide a review on the basic concepts of antioxidant-pro-oxidant interaction in relation to its effects on the eye.  相似文献