Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation     

L. Yu  S. M. Grist  S. S. Nasseri  E. Cheng  Y.-C. E. Hwang  C. Ni  K. C. Cheung 《Biomicrofluidics》2015,9(2)

Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since they may provide a better model of the tumor than conventional monolayer culture. Moreover, tumor cell interaction with the extracellular matrix can determine cell organization and behavior. In this work, a microfluidic system was used to form cell-laden core-shell beads which incorporate elements of the extracellular matrix and support the formation of multicellular spheroids. The bead core (comprising a mixture of alginate, collagen, and reconstituted basement membrane, with gelation by temperature control) and shell (comprising alginate hydrogel, with gelation by ionic crosslinking) were simultaneously formed through flow focusing using a cooled flow path into the microfluidic chip. During droplet gelation, the alginate acts as a fast-gelling shell which aids in preventing droplet coalescence and in maintaining spherical droplet geometry during the slower gelation of the collagen and reconstituted basement membrane components as the beads warm up. After droplet gelation, the encapsulated MCF-7 cells proliferated to form uniform spheroids when the beads contained all three components: alginate, collagen, and reconstituted basement membrane. The dose-dependent response of the MCF-7 cell tumor spheroids to two anticancer drugs, docetaxel and tamoxifen, was compared to conventional monolayer culture.  相似文献   

Referee acknowledgment for 2014     

Hsueh-Chia Chang  Leslie Y. Yeo 《Biomicrofluidics》2015,9(1)

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题目：一种支持产品平台设计的多准则模块划分方法   总被引：2，自引：0，他引：2  

Wei WEI  Ang LIU  Stephen C.Y.LU  Thorsten WUEST 《浙江大学学报(A卷英文版)》2015,(1):1-10

目的：研究多准则约束下的产品模块划分方法,为企业建立稳健的模块化产品平台奠定基础。方法：采用改进的多目标进化算法对建立的多准则模块划分数学模型求解,并采用模糊集合评价机制进行最优解的寻取,得到基于多准则模块划分方法的产品模块划分结果。结论：通过改进的多目标进化算法求解多准则模块划分模型,能够得到有效支持产品平台设计的产品模块划分方案。通过与已有优化方法的比较验证了本文提出的多准则模块划分方法的优越性。  相似文献   

Relationship Reciprocation Modulates Resource Allocation in Adolescent Social Networks: Developmental Effects          下载免费PDF全文

Stephanie Burnett Heyes  Yeou‐Rong Jih  Per Block  Chii‐Fen Hiu  Emily A. Holmes  Jennifer Y. F. Lau 《Child development》2015,86(5):1489-1506

Adolescence is characterized as a period of social reorientation toward peer relationships, entailing the emergence of sophisticated social abilities. Two studies (Study 1: N = 42, ages 13–17; Study 2: N = 81, ages 13–16) investigated age group differences in the impact of relationship reciprocation within school‐based social networks on an experimental measure of cooperation behavior. Results suggest development between mid‐ and late adolescence in the extent to which reciprocation of social ties predicted resource allocation. With increasing age group, investment decisions increasingly reflected the degree to which peers reciprocated feelings of friendship. This result may reflect social‐cognitive development, which could facilitate the ability to navigate an increasingly complex social world in adolescence and promote positive and enduring relationships into adulthood.  相似文献   

论苏童笔下的“颂莲”式女性     

周茜  王艳芳 《乐山师范学院学报》2015,(7)

苏童是少数几个尤其擅长写女性的男性作家。他对女性的心理描写和细节把握都处理得恰到好处,非常自然。他笔下的女性一改过往的温柔善良、知书达理,相反的,大多都阴暗猥琐、粗俗野蛮、善于妒忌,非常具有特色,成为中国当代女性小说的独有景观。苏童的几个从女性视角描写的女主人公具有很多的共同特征,他将这些女性称为“颂莲”式女性。  相似文献   

Fish-out-of-office: How managerialised university conditions make administrative knowledge inaccessible to academics     

Anita Louise Wheeldon  Stephen Jonathan Whitty  Bronte van der Hoorn 《Higher Education Quarterly》2023,77(2):342-355

Academics report feeling unable to cope in the managerialised university. To confirm these feelings are symptoms of managerialism's tightening grip, we use Bourdieusian concepts of field and capital to compare academics and professional staff experiential statements in an Australian university. We compare their field conditions and examine how their differences enable or hinder the accumulation of capital that defines their field. Findings show that managerialism requires professional staff to share work tasks and be on-campus, which enables them to accumulate the capital they require. Managerialism also permits and resources academics to working out-of-office to accumulate their required capital. Consequentially though, university operational knowledge becomes informal and only accessible to professional staff who accumulate the required social capital to access it. Professional staff are thus fish-in-water; easily accumulating social capital through day-to-day activities. But academics become fish-out-of-water (office); they flounder to access operational knowledge, which leads to feelings of not coping.  相似文献   

Hands-On: Investigating the role of physical manipulatives in spatial training     

Katie A. Gilligan-Lee  Zachary C. K. Hawes  Ashley Y. Williams  Emily K. Farran  Kelly S. Mix 《Child development》2023,94(5):1205-1221

Studies show that spatial interventions lead to improvements in mathematics. However, outcomes vary based on whether physical manipulatives (embodied action) are used during training. This study compares the effects of embodied and non-embodied spatial interventions on spatial and mathematics outcomes. The study has a randomized, controlled, pre-post, follow-up, training design (N = 182; mean age 8 years; 49% female; 83.5% White). We show that both embodied and non-embodied spatial training approaches improve spatial skills compared to control. However, we conclude that embodied spatial training using physical manipulatives leads to larger, more consistent gains in mathematics and greater depth of spatial processing than non-embodied training. These findings highlight the potential of spatial activities, particularly those that use physical materials, for improving children's mathematics skills.  相似文献   

How do thinking styles and STEM attitudes have effects on computational thinking? A structural equation modeling analysis     

Haozhe Jiang  A. Y. M. Atiquil Islam  Xiaoqing Gu  Jia Guan 《科学教学研究杂志》2024,61(3):645-673

Computational thinking (CT) is vital for success in numerous domains. However, the nature, definition, and scope of CT are ill-defined, and research on how best to develop CT is very limited. This study focused on how thinking styles and STEM attitudes have effects on computational thinking. Using a proportionate stratified random sampling procedure, 1195 students from two universities were surveyed. A structural equation modeling analysis showed that students' thinking styles and STEM attitudes directly predicted their computational thinking skills and that thinking styles mediated the relationship between STEM attitudes and computational thinking skills. Thinking styles and STEM attitudes are strong predictors of CT skills. Based on the results, we recommended that the conceptualization of CT be broadened to reflect its trans-disciplinary nature within the context of STEM education. This study adds to the limited theoretical understanding of CT and CT-predictors in higher education, which has been studied much less than in K-12 education.  相似文献   

Artificial intelligence and the Journal of Research in Science Teaching     

Troy D. Sadler  Felicia Moore Mensah  Jonathan Tam 《科学教学研究杂志》2024,61(4):739-743

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Reconciling Political and Economic Goals in the Net Neutrality Debate     

Johannes M. Bauer  Jonathan A. Obar 《The Information Society》2014,30(1):1-19

The net neutrality debate is one of several sites of struggles to adapt and redefine rights and obligations of stakeholders in the Internet-centric communications system. Its outcomes will shape the future of the Internet and influence the level and distribution of its benefits. We identify key values that drive the net neutrality discussion and examine the governance mechanisms proposed to achieve these objectives. We find that there is no single policy instrument that allows realization of the range of valued political and economic objectives simultaneously. Contrary to some of the claims advanced in the current debate, safeguarding multiple goals requires a combination of instruments that will likely involve government and nongovernment measures. Furthermore, promoting goals such as the freedom of speech, political participation, investment, and innovation calls for complementary policies.  相似文献