Effects of hydraulic pressure on cardiomyoblasts in a microfluidic device     

Yu-Fang Hsiao  Huei-Jyuan Pan  Yi-Chung Tung  Chien-Chang Chen  Chau-Hwang Lee 《Biomicrofluidics》2015,9(2)

We employed a microfluidic device to study the effects of hydraulic pressure on cardiomyoblast H9c2. The 170 mm Hg pressure increased the cellular area and the expression of atrial natriuretic peptide. With the same device, we demonstrated that the effects of hydraulic pressure on the cardiomyoblast could be reduced by the inhibitor of focal adhesion kinase. This mechanical–chemical antagonism could lead to a potential therapeutic strategy of hypertension-induced cardiac hypertrophy.  相似文献   

An integrated microfluidic system for screening of phage-displayed peptides specific to colon cancer cells and colon cancer stem cells     

Yu-Jui Che  Huei-Wen Wu  Lien-Yu Hung  Ching-Ann Liu  Hwan-You Chang  Kuan Wang  Gwo-Bin Lee 《Biomicrofluidics》2015,9(5)

Affinity reagents recognizing biomarkers specifically are essential components of clinical diagnostics and target therapeutics. However, conventional methods for screening of these reagents often have drawbacks such as large reagent consumption, the labor-intensive or time-consuming procedures, and the involvement of bulky or expensive equipment. Alternatively, microfluidic platforms could potentially automate the screening process within a shorter period of time and reduce reagent and sample consumption dramatically. It has been demonstrated recently that a subpopulation of tumor cells known as cancer stem cells possess high drug resistance and proliferation potential and are regarded as the main cause of metastasis. Therefore, a peptide that recognizes cancer stem cells and differentiates them from other cancer cells will be extremely useful in early diagnosis and target therapy. This study utilized M13 phage display technology to identify peptides that bind, respectively, to colon cancer cells and colon cancer stem cells using an integrated microfluidic system. In addition to positive selection, a negative selection process was integrated on the chip to achieve the selection of peptides of high affinity and specificity. We successfully screened three peptides specific to colon cancer cells and colon cancer stem cells, namely, HOLC-1, HOLC-2, and COLC-1, respectively, and their specificity was measured by the capture rate between target, control, and other cell lines. The capture rates are 43.40 ± 7.23%, 45.16 ± 7.12%, and 49.79 ± 5.34% for colon cancer cells and colon cancer stem cells, respectively, showing a higher specificity on target cells than on control and other cell lines. The developed technique may be promising for early diagnosis of cancer cells and target therapeutics.  相似文献   

How liberals and conservatives respond to feasibility and desirability appeals in anti-tobacco campaigns     

Younghwa Lee  Sukki Yoon  Seungwoo Chun  Chanmo Park  Kacy Kim 《亚洲交流杂志》2019,29(1):55-72

Using two experiments, this study aims to investigate how politically liberal or conservative message recipients respond to anti-tobacco appeals. The results show that in Study 1, respondents were exposed to a message about price policy. In Study 2, they were exposed to a message about a warning policy. In both studies, liberal participants more favorably evaluated anti-tobacco messages emphasizing feasibility rather than desirability, whereas conservative participants more positively evaluated messages emphasizing desirability rather than feasibility. Implications for policymakers and marketers are discussed.  相似文献   

Helping Taiwanese Graduate Students Help Themselves: Applying Corpora to Industrial Management English as a Foreign Language Academic Reading and Writing     

Barry Lee Reynolds 《学校用计算机》2015,32(3-4):300-317

Lack of knowledge in the conventional use of vocabulary and multiword patterns in one's respective field of expertise causes Taiwanese students to produce academic writing that is markedly “non-nativelike.” This is because Taiwanese students are first and foremost second language readers and often have difficulty “picking up on” the regularly encountered language patterns found in scholarly texts. As one step in determining a solution to this problem, this article reports on a pilot implementation encouraging graduate Taiwanese students for whom English is a foreign language to use self-editing techniques in their own field-specific academic writing by use of a corpus tool. Classroom interviews, draft comparisons, and individual stimulated recall interviews showed a difference in acceptance and success after using the corpus tool (StringNet Navigator) as an aid to self-editing academic English writing. The corpus tool's functions, results of its use by industrial management graduate students for self-editing academic writing, and pedagogical implications are described.  相似文献   

Youths’ socialization to work and school within the family     

Bora Lee  Erik J. Porfeli 《International Journal for Educational and Vocational Guidance》2015,15(2):145-162

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“What would you like me to do? Lie to you?” Teacher education responsibilities to LGBTI students     

Debora Lee  Vicki M. Carpenter 《Asia-Pacific Journal of Teacher Education》2015,43(2):169-180

The experiences of lesbian, gay, trans (The use of trans with an asterisk avoids the use of transsexual or transgender and promotes recognition of the inadequacy of such labels), bisexual and intersex (LGBTI) student teachers were recently investigated at a New Zealand faculty of education. Student teachers studying in early childhood education and care, primary and secondary initial teacher education (ITE) were asked about their perceptions of LGBTI visibility and inclusion. Methods used were online questionnaires, focus groups and individual interviews. While the study encompassed all aspects of the ITE programme, this work uses one specific question about practicum from the questionnaire, and findings related to practicum and teaching from the focus groups and individual interviews. In this article, the experiences of LGBTI (those who identified as non-heterosexual) student teachers and “straight” (those who identified as heterosexual) are discussed. Findings suggest that both faculty and practicum settings are heteronormative and indicate that LGBTI student teachers felt uncertain about their safety. Both LGBTI and straight students felt they had not been given adequate preparation to manage the complexities of diverse sexualities on practicum or in their future teaching. We argue that addressing heteronormativity in ITE will better prepare student teachers for the rich diversity of students and families they will encounter in their teaching.  相似文献   

Toward an educational view of scaling: sufficing standard and not a gold standard     

David Hung  Shu-Shing Lee  Longkai Wu 《Educational Research for Policy and Practice》2015,14(1):77-91

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DISTRIBUTIVE PARTITIONING OPERATION IN MATHEMATICAL SITUATIONS INVOLVING FRACTIONAL QUANTITIES     

Soo Jin Lee  Jaehong Shin 《International Journal of Science and Mathematics Education》2015,13(2):329-355

In the present study, we describe a participating student’s (Carol’s) distributive partitioning scheme and operations along with Steffe’s and his colleagues’ studies about children’s constructions of fraction knowledge as a particular model of mathematical learning. Analysis of Carol’s mathematical behaviors indicates that an operationally common mathematical behavior (distributive partitioning operation) was revealed in various mathematical problem situations such as fraction multiplication, fraction division, and multiplicative transformation between fractional quantities. It both provides a rationale for why becoming versed in one mathematical subject could facilitate working with another mathematical subject and also implies the necessity of describing and defining students’ mathematical behaviors from an operational view of knowledge, which might lead to building foundations of a substantial cognitive map for students’ mathematical development.  相似文献   

Global pedigree and national imperative: Hong Kong universities’ response to China’s grand strategies     

Lo  William Yat Wai  Lee  Jack T.  Abdrasheva  Dana 《Higher Education》2022,84(1):141-157

Higher Education - Literature offers a theoretical framework exemplifying the inherent tensions between “becoming Chinese” and “remaining global” in the evolution of the...  相似文献   

The Effects of Perfectionism and Dispositional Self-focused Attention of Novice Counselor on Psychological Burnout: Moderated Mediating Effects of Cognitive Emotion Regulation Strategies     

Kwon  Wonju  Ha  Jung Hee  Jang  Hansori  Lee  Donghun 《International journal for the advancement of counseling》2022,44(2):283-297

International Journal for the Advancement of Counselling - The current study investigated the mediation effect of self-focused attention on the relationship between South Korean novice...  相似文献