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11.
Ramesh Chandra Manisha Tiwari Ritu Aneja Sujata K. Dass Archana Sharma 《Indian journal of clinical biochemistry : IJCB》2000,15(2):148-154
We investigated the effect of cholesterol and the metalloporphyrins cobalt mesoporphyrin (CoMP) and chromium protoporphyrin
(CrPP) on phospholipase A2 (PLA2) activity and the consequent hepatic mitochondrial stability as well as on lipid concentrations. Our studies revealed that
on administration of cholesterol, CrPP, CoMP as well as simultaneous adminstration of cholesterol and CrPP, there was an inhibition
of PLA2 activity. These moieties may therefore, be agents for preventing destabilisation of the mitochondrial membrane and the consequent
pathological conditions which may arise due to membrane lysis. Our results revealed that cholesterol administration increased
phospholipid concentration, albeit by modest amounts. Although the independent administration of metalloporphyrins led only
to minor elevations in phospholipid concentration, the simultaneous administration of cholesterol and CrPP generated a steep
elevation in the concentration of total phospholipid. Since cholesterol inhibits PLA2 activity it has the potential of being therapeutic agent for preventing the pathological conditions which may arise due to
membrane lysis. 相似文献
12.
Mridula Mahajan Nitika Tiwari Ritu Sharma Sukhraj Kaur Neetirajan Singh 《Indian journal of clinical biochemistry : IJCB》2013,28(1):51-54
Reactive oxygen species (ROS) cause damage to the DNA producing mutations and formation of tumours such as carcinoma of breast. Tumour cells are known to produce ROS at a greater pace than the non-transformed cells. The increased production of reactive oxygen species causes oxidative stress leading to cell proliferation and hence increased inflammatory conditions. The present study was aimed to investigate the role of oxidative stress in the pathogenesis of breast cancer. Females suffering from breast cancer had significantly decreased Superoxide dismutase (SOD) and reduced glutathione (GSH) levels in comparison to normal females. The compromised antioxidant defence system produces the oxidative stress which in turn creates the inflammatory response shown by concomitant increased adenosine deaminase (ADA) activity in female patients. ADA diminishes the protective molecule adenosine. There were significant variations (p < 0.01) in ADA activity with different clinical stages (stage 1–4) of breast cancer suggesting thereby that estimation of ADA activity can be used as a diagnostic tool to detect the stage of cancer along with cytological studies. Mastectomy was performed and post-operatively serum SOD and ADA activity and plasma GSH levels were estimated. There was a statistically significant increase in activity of SOD and levels of GSH while serum ADA activity decreased significantly, suggesting thereby that oxidative stress is responsible for increased cell proliferation and hence the inflammatory conditions in CA breast that got ameliorated post-operatively. 相似文献
13.
Ghizal Fatima Siddharth Kumar Das Abbas Ali Mahdi Nar Singh Verma Faizan Haider Khan Amit Mani Kumar Tiwari Tabrez Jafer Baby Anjum 《Indian journal of clinical biochemistry : IJCB》2013,28(2):181-184
Fibromyalgia syndrome (FMS) patients have disturbed sleep patterns which may lead to altered circadian rhythm in serum cortisol secretion. The aim of this study was to assess circadian changes, if any, in serum cortisol levels in female patients with FMS. Cortisol levels were estimated every 6 h during 24 h period; in 40 female patients satisfying ACR criteria for FMS (Age 36.4 ± 9.9), and 40 healthy females without FMS (Age 33.8 ± 11.1). A significant difference in the night time serum cortisol level was observed among the patients and control groups (patients, 12.9 ± 9.7 controls 5.8 ± 3.0; p < 0.01). However, no significant difference was found in serum cortisol levels in patients and control groups in the morning (patients, 28.4 ± 13.2 controls, 27.6 ± 14.5; p > 0.05), afternoon (patients, 14.4 ± 5.6 controls, 14.0 ± 6.6; p > 0.05) and evening hours (patients, 10.9 ± 5.8 controls, 8.9 ± 3.6; p > 0.05). It could be concluded that there is an abnormality in circadian secretion of cortisol in female FMS patients. 相似文献
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15.
Electrowetting with alternate voltage (AC) creates azimuthal flow vortices inside sessile droplets. These flow vortices can be controlled by introducing pinning sites at the contact line. When the frequency of the applied AC voltage is gradually ramped from a few hundreds of hertz to a few tens of kilohertz the azimuthal flow vortices contract and move towards the contact line near the pinning site. Dispersed particles in the liquid are collected in the center of these vortices leading to an increase in the local particle concentration by up to more than one order of magnitude. We provide a qualitative explanation for symmetry of the flow patterns within the drops and discuss possible scenarios explaining the particle collection and preconcentration. 相似文献
16.
Rina Shah Ajay Das Ishwar Desai Ashwini Tiwari 《Journal of Research in Special Educational Needs》2016,16(1):34-45
This study was undertaken to determine the concerns of primary school teachers about the inclusion of students with disabilities in Ahmedabad, India. A total of 560 teachers, working in government‐run schools, returned the completed survey. A two‐part questionnaire was used in this study. Part 1 gathered information relating to personal and professional characteristics of the teachers. Part 2 was a 21‐item Likert scale titled Concerns about Inclusive Education – Gujarati. The major finding of the study was that the teachers in Ahmedabad were moderately concerned about including students with disabilities in their classrooms. The teachers were most concerned about lack of infrastructural resources and least concerned about lack of social acceptance of students with disabilities in inclusive education classrooms. Significant differences existed in teacher concerns based on the following background variables: gender, qualifications in special education, teaching experience and number of students with disabilities in class. A number of implications are discussed to address teacher concerns for inclusive education in India. 相似文献
17.
Kiran Dahiya A. D. Tiwari Vijay Shankar Simmi Kharb Rakesh Dhankhar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):157-160
Phototherapy has been related to increased oxidative stress and lipid peroxidation. In the present study, thirty full term
jaundiced neonates with appropriate weight were analyzed before and after completion of phototherapy for malondialdehyde (MDA),
reduced glutathione (GSH), total thiols, vitamin C and superoxide dismutase (SOD) levels in hemolysate and albumin levels
in plasma. These parameters were analyzed in cord blood samples of 20 healthy neonates as control. It was observed that levels
of MDA were elevated significantly (p<0.001) in patients as compared to controls and that the levels increased significantly
after phototherapy (p<0.001). Levels of SOD were also found to be increased significantly as compared to controls and the
levels rose after phototherapy (p<0.001). On the other hand, the levels of non-enzymatic antioxidants such as GSH, total thiols
and vitamin C were significantly low (p<0.001) as compared to controls and the levels decreased significantly after phototherapy
(p<0.001). The plasma albumin levels also were found to be decreased significantly after phototherapy (p<0.01). Therefore,
phototherapy increases oxidative stress and should be used with care. 相似文献
18.
B. Sivasankar K. R. Raju V. Anand S. Malu S. Padmanabhan S. C. Tiwari Nibhriti Das L. M. Srivastava 《Indian journal of clinical biochemistry : IJCB》1999,14(2):237-240
A decrease in the membrane anchored erythrocyte complement receptor 1 (CR1) is reported as an acquired phenomenon in a number
of inflammatory and autoimmune diseases with concomitant rise in soluble CR1 (sCR1) levels in plasma. There is a need to establish
the normal range of sCR1 in Indian adults to assess the function and disease association of this protein. The plasma sCR1
levels of 50 healthy individuals have been estimated by an indigenously developed sandwich ELISA. sCR1 levels from 26 patients
suffering from nephropathies had also been assayed which was much higher than the normal controls. This observation suggests
sCR1 as a potential market for the assessment of disease activity in nephropathies. 相似文献
19.
Singh TD Barbhuiya MA Gupta S Shrivastav BR Jalaj V Agarwal N Tiwari PK 《Indian journal of clinical biochemistry : IJCB》2011,26(2):146-153
We have studied the expression of lactate dehydrogenase and its isoforms in gall bladder cancer, cholelithiasis and chronic
cholecystitis. Quantitative and qualitative assays of lactate dehydrogenase and its various isoforms were carried out in the
blood sera of patients and healthy controls along with parallel estimation of various liver function test enzymes. Statistical
analysis was done using the software Graph Pad Prism. Significantly high expression of lactate dehydrogenase along with alkaline
phosphatase and total bilirubin (P ≤ 0.05) was observed in all the three clinical conditions as compared to controls. LDH showed an increasing trend from stage
I to stage IV of GBC indicating a significant positive association with the disease progression. The levels of LDH 3 and 4
isoforms appeared significantly more elevated in GBC than cholelithiasis or chronic cholecystitis. We suggest that a careful
estimation of total LDH and its isoforms 3 and 4 alone or along with alkaline phosphatase and total bilirubin during different
clinical stages, like chronic cholecystitis, cholelithiasis and GBC, may prove to be a potentially useful biomarker in the
prognostic management of gall bladder diseases, specifically GBC. 相似文献
20.
Arun Kumar Singh Sunita Tiwari Abhishek Gupta Kamla Kant Shukla Kumar Gaurav Chhabra Achileshwar Pandey Aditya Bhushan Pant 《Indian journal of clinical biochemistry : IJCB》2015,30(3):255-262
Metabolic syndrome (MetS) is a cluster of interrelated common clinical disorders. The role of resistin in insulin sensitivity and MetS is controversial till date. So, the aim of the present study was to investigate the relationship of plasma resistin levels with markers of the MetS in Indian subjects. In a case control study, total 528 subjects were selected for the study. 265 (194 male and 71 female) were cases (with MetS) and 263 (164 male and 99 female) were controls (without MetS). Required anthropometric measurements and calculations were carried out accordingly. All the Biochemical estimations were carried out according to standard protocol. Resistin level was measured by the standard protocol (By ELISA i.e. enzyme linked immunosorbent assay) as illustrated in the kit. Insulin level was also measured by the standard protocol as illustrated in the kit and insulin resistance was calculated by the standard procedures. Plasma resistin levels were significantly higher in cases compared with controls (male = 13.05 ± 4.31 vs. 7.04 ± 2.09 ng/ml; p ≤ 0.001 and female = 13.53 ± 4.14 vs. 7.42 ± 2.30 ng/ml; p ≤ 0.001). Plasma resistin levels were well correlated with waist circumference, glucose, triglycerides, waist/hip ratio, systolic and diastolic blood pressure, high density lipoprotein, total cholesterol, serum low density lipoprotein, serum very low density lipoprotein, insulin and insulin resistance. Plasma resistin levels were elevated in presence of the MetS and were associated with increased metabolic risk factors. 相似文献