Liu Ai-jun  Furusato Bungo  Ravindranath Lakshmi  Chen Yong-mei  Srikantan Vasanta  Mcleod David G.  Petrovics Gyorgy  Srivastava Shiv 《Journal of Zhejiang University. Science. B》2007,8(12):853-859

Objective To investigate molecular alterations associating with prostate carcinoma progression and potentially provide information toward more accurate prognosis/diagnosis. Methods A set of laser captured microdissected (LCM) specimens from 300 prostate cancer (PCa) patients undergoing radical prostatectomy (RP) were defined. Ten patients representing “aggressive” PCa, and 10 representing “non-aggressive” PCa were selected based on prostate-specific antigen (PSA) recurrence, Gleason score, pathological stage and tumor cell differentiation, with matched patient age and race between the two groups. Normal and neoplastic prostate epithelial cells were collected with LCM from frozen tissue slides obtained from the RP specimens. The expressions of a panel of genes, including NPY, PTEN, AR, AMACR, DD3, and GSTP1, were measured by quantitative real-time RT-PCR (TaqMan), and correlation was analyzed with clinicopathological features. Results The expressions of AMACR and DD3 were consistently up-regulated in cancer cells compared to benign prostate epithelial cells in all PCa patients, whereas GSTP1 expression was down regulated in each patient. NPY, PTEN and AR exhibited a striking difference in their expression patterns between aggressive and non-aggressive PCas (P=0.0203, 0.0284, and 0.0378, respectively, Wilcoxon rank sum test). The lower expression of NPY showed association with “aggressive” PCas based on a larger PCa patient cohort analysis (P=0.0037, univariate generalized linear model (GLM) analysis). Conclusion Despite widely noted heterogeneous nature of PCa, gene expression alterations of AMACR, DD3, and GSTP1 in LCM-derived PCa epithelial cells suggest for common underlying mechanisms in the initiation of PCa. Lower NPY expression level is significantly associated with more aggressive clinical behavior of PCa; PTEN and AR may have potential in defining PCa with aggressive clinical behavior. Studies along these lines have potential to define PCa-associated gene expression alterations and likely co-regulation of genes/pathways critical in the biology of PCa onset/progression. Project supported by the Center for Prostate Disease Research, and the Henry M. Jackson Foundation for the Advancement of Military Medicine, Rockville, MD, USA  相似文献   

Remembering Newton     

Srivastava  P. K. 《Resonance》2007,12(8):85-96

This is the story of Isaac Newton, the scientist and the man. Life and work of Newton reveals how thought processes are determined by the cultural-philosophical bias of the times.  相似文献   

Evaluation of Oxidative Stress and Antioxidant Status in Pregnant Anemic Women     

Tiwari AK  Mahdi AA  Zahra F  Chandyan S  Srivastava VK  Negi MP 《Indian journal of clinical biochemistry : IJCB》2010,25(4):411-418

The present study was conducted to investigate the oxidant–antioxidant status in iron deficient pregnant anemic women. One hundred thirty pregnant women with iron deficiency anemia (IDA) were divided into three groups, namely mild (50), moderate (50) and severe (30) anemic along with pregnant healthy women as controls (50). The complete blood count, plasma lipid peroxidation products, enzymatic and non-enzymatic antioxidants were measured according to respective protocols. The levels of complete blood count, iron, ferritin along with antioxidant enzymes namely catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and reduced glutathione were significantly reduced in all IDA groups. However, the level of oxidized glutathione, lipid peroxides, protein carbonyls, conjugated dienes were found significantly increased in all anemic patients. Antioxidant vitamins, namely C, E and A were also found significantly decreased in IDA patients. On the basis of our results, it may be concluded that IDA tends to increase the pro-oxidant components, which may result in various complications including peroxidation of vital body molecules resulting in increased risk for pregnant women as well as fetus.  相似文献   

Polymorphisms in the vitamin D receptor and the androgen receptor gene associated with the risk of urolithiasis     

Rama Devi Mittal  D. K. Mishra  P. Srivastava  P. Manchanda  H. K. Bid  R. Kapoor 《Indian journal of clinical biochemistry : IJCB》2010,25(2):119-126

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Effect of Arsenic Contaminated Drinking Water on Human Chromosome: A Case Study     

Asha Lata Singh  Vipin Kumar Singh  Anushree Srivastava 《Indian journal of clinical biochemistry : IJCB》2013,28(4):422-425

Arsenic contamination of ground water has become a serious problem all over the world. Large number of people from Uttar Pradesh, Bihar and West Bengal of India are suffering due to consumption of arsenic contaminated drinking water. Study was carried out on 30 individuals residing in Ballia District, UP where the maximum concentration of arsenic was observed around 0.37 ppm in drinking water. Blood samples were collected from them to find out the problem related with arsenic. Cytogenetic study of the blood samples indicates that out of 30, two persons developed Klinefelter syndrome.  相似文献   

Simple models for the 100 meter dash     

Priyanka deSouza  Vijay A. Singh 《Resonance》2012,17(6):592-603

In this section of Resonance, we invite readers to pose questions likely to be raised in a classroom situation. We may suggest strategies for dealing with them, or invite responses, or both. ??Classroom?? is equally a forum for raising broader issues and sharing personal experiences and viewpoints on matters related to teaching and learning science.  相似文献   

A Summer Academic Research Experience for Disadvantaged Youth     

Cathryn Kabacoff  Vasudha Srivastava  Douglas N. Robinson 《CBE life sciences education》2013,12(3):410-418

Internships are an effective way of connecting high school students in a meaningful manner to the sciences. Disadvantaged minorities have fewer opportunities to participate in internships, and are underrepresented in both science, technology, engineering, and mathematics majors and careers. We have developed a Summer Academic Research Experience (SARE) program that provides an enriching academic internship to underrepresented youth. Our program has shown that to have a successful internship for these disadvantaged youth, several issues need to be addressed in addition to scientific mentoring. We have found that it is necessary to remediate and/or fortify basic academic skills for students to be successful. In addition, students need to be actively coached in the development of professional skills, habits, and attitudes necessary for success in the workplace. With all these factors in place, these youths can become better students, compete on a more level playing field in their internships, and increase their potential of participating actively in the sciences in the future.  相似文献   

Diffusion of Innovations: Communication Evolution and Influences     

Jatin Srivastava  Jennifer J. Moreland 《The Communication Review》2013,16(4):294-312

Diffusion of innovations research has altered the research trajectories of those in the field of communication. In this article, the authors briefly explore the evolution of diffusion research, in terms of theoretical influences and applied research advances, and then address how the evolution of diffusion of innovations provided the impetus for a communication discipline identity search. An explication of how diffusion of innovations can be viewed as mechanistically affecting media convergence is presented. This piece closes with a call to return to diffusion research, especially given the face of current communication vehicles and media convergence.  相似文献   

A Microcomputer Interfacing Course for Computer Science Majors     

R. P. Srivastava 《Computer Science Education》2013,23(1):61-80

This paper describes a microcomputer interfacing course for computer science students. Students build simple logic circuits to interface a microcomputer with real‐world devices, such as stepper motor and digital displays, and program the computer to monitor and control these devices. Programs are written in Pascal and assembly language. The emphasis is placed on applications rather than on theory. This course is especially designed for computer science students who are proficient in software programming but have little or no experience in electronics. The experiments described here have been developed on the Apple ft microcomputer, but they can be easily adapted to other microcomputers.  相似文献   

The First Digit 1     

Tanya Kaushal Srivastava 《Resonance》2013,18(12):1073-1085

Benford’s Law or The First Digit Law as it is commonly known has been a fascination to many generations. This counter-intuitive law proposes that given a sequence of numbers (usually from a data set like length of rivers, height of mountains, populations of nations or any source of data from real life), the first digit is ‘1’ roughtly 30% of the time. Many mathematical sequences, such as Fibonacci sequences also follow Benford’s Law. Benford’s Law has some interesting applications, especially in fraud detection!  相似文献