Prevalence of insulin resistance in first degree relatives of type-2 diabetes mellitus patients: A prospective study in north Indian population     

Arvind Kumar  Poornima Tewari  Sibasis S. Sahoo  Arvind Kumar Srivastava 《Indian journal of clinical biochemistry : IJCB》2005,20(2):10-17

A total of 172 first degree relatives (FDRs) and 178 controls were included in this study. All the cases and controls were subjected to various anthropometric measurements, fasting and postprandial glucose estimation, fasting insulin measurement and fasting lipid profile. Results revealed the prevalence of Impaired Fasting Glucose (IFG) (cases 37% Vs controls 11.6%), Impaired Glucose Tolerance (IGT) (cases 34.3% Vs controls 11.2%) and diabetes (cases 11.05% controls 3.37%) was significantly higher in first degree relatives. Insulin resistance was measured using various methods, which included fasting plasma insulin (FPI), Homeostasis Model Assessment for Insulin Resistance (HOMA_IR), insulin sensitivity index (ISI) (Mffm/l). Prevalence of insulin resistance (Insulin Resistance) as observed comparing FPI and HOMA_IR in cases and controls was 43.6% and 11.24% (P=0.005) and 37.8% and 12.47% (P=0.000) respectively. Prevalence of IR (Insulin Resistance) observed in cases having Normal Glucose Tolerance (NGT), Impaired Fasting Glucose (IFG), Impaired Glucose Tolerance (IGT) and diabetes mellitus measuring FPI Vs HOMA_IR was 37.5% vs 30.2%, 45% vs 40%, 38.98% vs 37.28% and 36.84% vs 31.57% as accordingly. However, ISI (Mffm/l) was not found to be a promising index for IR due to its poor specificity. Though HOMA is taken as gold standard for measurement of IR globally, our study observed fasting plasma insulin representing high sensitivity (89.7%) and specificity (93.3%) as compared to HOMA. Thus FPI had emerged in this work as a simple and reliable test for diagnosing insulin resistance across the population susceptible to develop diabetes including FDRs.  相似文献   

Serum Malondialdehyde (MDA) in relation to lipidemic status and atherogenic index     

J. Bhattacharjee  D. K. Srivastava 《Indian journal of clinical biochemistry : IJCB》1993,8(1):12-15

Lipid peroxidation (MDA) was studied spectrophotometrically in relation to lipidemic status and atherogenic index in IHD and IHD risk subjects. It was found to be significantly elevated compared to control subjects (P<0.005) in both the groups, showing a linear correlation with cholesterolemic status. The values were found to run parallel to atherogenic index. In 10% cases with borderline atherogenic index the lipid peroxidation was found to be highly elevated. It is speculated that it may have a future role as a marker for IHD risk specially in premature group.  相似文献   

Oxygen derived free radicals in clinical context     

V. K. Gupta  V. Mallika  Yashika Gupta  D. K. Srivastava 《Indian journal of clinical biochemistry : IJCB》1992,7(1):3-10

Oxygen derived free radicals have been implicated in a number of clinical disorders including atherosclerosis (1), ischemic heart disease (IHD) (2), post ischemic reperfusion injury (3) and respiratory distress syndrome (4). These radical are generated by sequential reduction of molecular oxygen; the primary product being superoxide anion (O₂ ^.−) which is subsequently reduced to hydrogen peroxide (H₂O₂), hydroxy1 radical (OH^.) and singlet oxygen (¹O₂). However the evidence for ODFR induced cell damage in various clinical disorders is still debated and rests largely on free radical scavenging studies, through electron paramagnetic resonance spectroscopic (EPRS) studies have provided direct evidence for ODFR generation following coronary artery ligation (5). By definition, a free radical is an atom, ion or molecule with one or more unpaired electrons (the presence of unpaired electron in a free radical being represented by a superscribed bold dot-R^.) and may be formed as a result of homolytic fission of a covalent bond or by electron transfer reactions, and may have cationic (NH₃ ⁺), anionic (O₂ ^.−) or neutral (NO) characteristics. The most important in vivo source for these radical species have been found to be univalent biochemical redox reactions involving oxygen. (a) A:B→A^.+B^. (b) A:+B→A^.+B^.  相似文献   

Myasthenia gravis with thymoma presenting with thyrotoxicosis     

D. K. Srivastava  V. K. Gupta  M. Gupta  V. Malika 《Indian journal of clinical biochemistry : IJCB》1993,8(1):75-75

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Binding and degradation of native and acetylated low density lipoproteins by monocyte derived macrophages of normal and hypercholesterolemic rabbits     

Sabari Das  Snehlata  L M Srivastava 《Indian journal of clinical biochemistry : IJCB》1997,12(1):59-62

Low density lipoprotein (LDL) was isolated from normal rabbits and was modified with acetic anhydride. Blood monocyte derived macrophages from normal and hypercholesterolemic (HC) rabbits were cultured, and on the 8th day the cells were incubated with native and modified LDL to study their binding and degradation. Macrophages from both normal and hypercholesterolemic rabbits express a limited number of receptors for normal plasma LDL whereas they exhibit a large number of receptors for acetyl LDL. There was no significant difference between binding and degradation of acetyl LDL by normal or hypercholesterolemic cells. However, binding and degradation of native LDL by monocytes of hypercholesterolemic cells were significantly less (p<0.0001) in comparison to binding and degradation of native LDL by normal cells indicating that there is a feedback inhibitory pathway in the cell that inhibits the synthesis of LDL receptors in the presence of excess LDL.  相似文献   

Polymorphism (C677T) in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene: A preliminary study on north Indian men     

S. Vasisht  R. Gulati  R. Narang  N. Srivastava  L. M. Srivastava  S. C. Manchanda  D. P. Agarwal 《Indian journal of clinical biochemistry : IJCB》2002,17(1):99-107

An elevated level of plasma homocysteine, sulfur containing amino acid generated through demethylation of methionine has been widely accepted as a risk factor for cardiovascular disease (CVD). The increase can result from genetic and/or nutrient related disturbances in the remethylation or transsulfuration pathways for homocysteine metabolism. A common mutation (C677T) in the gene encoding for the enzyme 5, 10-methylenetetrahydrofolate reductase (MTHFR) or deficiency of the B vitamins namely folic acid, B₁₂, B₆ can lead to hyperhomocysteinemia. In the present study, we have investigated the incidence of the (C677T) MTHFR polymorphism in the North Indian males. 141 angiographically proven coronary artery disease (CAD) patients and 55 age and sex matched healthy volunteers were examined for the association between MTHFR gene polymorphism and CAD. The MTHFR genotyping was performed using polymerase chain reaction (PCR) followed by restriction-isotyping with Hinf 1 endonuclease. A trend for higher ‘T’ allele frequency (0.19) was observed in patients than in controls (0.16). However no significant association was found between C677T mutation and CAD severity. The lack of statistical significance could be due to the small sample size studied. Hence a larger study including various ethnic groups is warranted.  相似文献   

Stress proteins     

K. K. Srivastava  Ganju Lilly 《Indian journal of clinical biochemistry : IJCB》1992,7(1):11-14

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Preparing for the inclusive classroom: changing teachers’ attitudes and knowledge     

Meenakshi Srivastava  Anke A. de Boer  Sip Jan Pijl 《Teacher Development》2017,21(4):561-579

India’s educational policy on inclusive education for students with disabilities is in sync with general global developments. Especially in the last couple of years there have been significant legislative achievements towards this endeavour. However, the implementation of inclusive education remains elusive. Besides this, teachers’ preparedness for inclusion has received little attention. This survey study had two aims. Firstly, we aimed at finding or constructing a reliable instrument to measure three aspects: the attitudes, knowledge about disability and inclusive teaching methods of regular schoolteachers. Secondly, we aimed to describe the outcomes of the three aspects. For attitudes and teaching methods we found a reliable instrument, which was selected for the usage in this study. We constructed and assessed the reliability of a scale in measuring knowledge about four disabilities: attention deficit hyperactivity disorder, dyslexia, intellectual disability and autistic spectrum disorder in pilot and main studies. The results revealed that teachers hold neutral attitudes towards inclusive education. Their knowledge about disabilities, however, was low, but knowledge about inclusive teaching methods was acceptable, which was a significant finding. The practical implications for preparing teachers to work towards inclusion are also discussed.  相似文献   

Kinetic analysis of LDL oxidation in IHD and IHD risk subjects in Indian population     

Yashika Gupta  V. Mallika  D. K. Srivastava 《Indian journal of clinical biochemistry : IJCB》2003,18(1):35-45

High plasma concentration of low-density lipoprotein (LDL) is associated with increased risk of atherosclerosis. Modified forms of LDL, especially oxidized LDL play a major role in its pathogenesis. This article gives detailed insight into the kinetics ofin vitro LDL oxidation by copper at different concentrations in normal and high-risk group subjects. Basal level of oxidatively modified LDL was significantly higher in ischaemic heart disease (IHD) and IHD hyperlipidemic subjects compared to normolipidemic and, hyperlipidemic control subjects, respectively. Derivatization of amino groups of apo-lipoprotein as monitored by estimating free amino groups concentration, was significantly higher in high-risk group and established IHD cases. Kinetics of oxidation was studied with two different concentrations of CuSO₄ (2.5 mM and 7.5 mM). thiobarbituric acid reactive substances (TBARS) level increases with time, and up to 95% oxidation was observed in 8 hr. About 60–65% less free amino groups were observed in native-LDL isolated from IHD patients compared to normal subjects. Study also showed an increase in two oxidative products studied, 20α-OH-cholesterol and 4-cholesten-3-one with oxidation time accompanied by corresponding decrease in LDL cholesterol. Increase in oxidative species was more evident in high-risk group and IHD patient. Basal level of oxidatively modified LDL measured in terms of TBARS was significantly higher in present study, strongly support that the extent of LDL oxidation monitored as TBARS and FAG level in circulating-LDL could be used as risk marker for high risk group.  相似文献   

Cadmium, carcinogen, co-carcinogen and anti carcinogen     

Ramesh Chandra  Sujata K. Dass  Priyanka Tomar  Manisha Tiwari 《Indian journal of clinical biochemistry : IJCB》2001,16(2):145-152

As a stress agent, inducing apoptosis and blocking it, Cd can have both helpful and harmful effects. The atmosphere is a thin envelope which makes the worid a global village. Cd is the most toxic metal in air. As both the first and second messenger of the stress response, it is synergistically toxic with all other stressors, including many other carcinogens. Elimination of Pb and its replacement with added benzene in gasoline appears to have increased the toxicity of atmospheric Cd. With scientific understanding of the molecular basis of Cd's role in carcinogenesis and anti-carcinogenesis, primary cancer prevention can be practiced by reducing Cd and chemical air pollution and educating the public on smoke cessation, healthy eating habits and stress reduction. Using the existing information on Cd and its effects, determinations could be made on established cancers so that individualized treatment protocols can be developed to improve patient care.  相似文献