Reduced glutathione: Importance of specimen collection     

Sarita Chavan  Laxmayya Sava  Vishal Saxena  Sandhya Pillai  Alka Sontakke  Digamber Ingole 《Indian journal of clinical biochemistry : IJCB》2005,20(1):150-152

Glutathione, the dominant intracellular thiol, plays an important protective role against oxidative stress. The accidental findings of increased reduced glutathione level postprandially as compared to post absorptive level prompted the design of present study. Reduced Glutathione levels were estimated in 50 healthy individuals in post absorptive and postprandial phase by taking whole blood in ACD bulb. Mean postprandial reduced Glutathione (9.60±3.39 μmole/gm of Hb) is significantly increased than mean postabsorptive level (5.53±0.88 μmole/gm of Hb; p<0.001). It also shows positive correlation (r=0.65) between these two GSH levels. So present study suggests that post absorptive specimen collection is preferable over random or postprandial as the former reflects the true basal level of reduced glutathione.  相似文献   

Acute and chronic toxicity studies on partially purified hypoglycemic preparation from water extract of bark ofFicus bengalensis     

S. Gupta  R. Shukla  K. M. Prabhu  S. Aggrawal  U. Rusia  P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》2002,17(1):58-63

Acute and chronic toxicity studies were conducted to assess toxicity of a partially purified preparation from the water extract of the bark ofFicus bengalensis, which was demonstrated in our earlier studies to have significant hypoglycemic and hypocholesteroiemic effect on alloxan induced, mild and severe diabetes in rabbits. LD₅₀ of this preparation was found to be ∼1 gm/kg in rats when given orally. For chronic toxicity studies 3 doses of aqueous preparation were given to 3 groups of rats. First group received 5 times ED₅₀ (50 mg/kg), second group 10 times ED₅₀ (100 mg/kg) and the third group 15 times ED₅₀ (150 mg/kg) for 3 months. Fourth group which served as control was given water. After three months, blood was collected for studying biochemical and hematological parameters. Blood glucose, serum cholesterol, liver and kidney function tests, haemoglobin, total and differential leukocyte count were determined. Animals were sacrificed and histopathological examination of liver, heart and kidneys was carried out. Results of the study showed that partially purified preparation fromFicus bengalensis is not toxic by all the above mentioned parameters.  相似文献   

Glycosylated proteins and serum lipid profile in complicated and uncomplicated NIDDM patients     

Bhalla Kapil  Shukla Rimi  V. P. Gupta  S. Pugazhenthi  K. M. Prabhu 《Indian journal of clinical biochemistry : IJCB》1995,10(2):57-61

In the present study, 58 diabetic patients (type II) comprising uncomplicated (27) and complicated (31) cases along with 38 healthy matched controls were investigated for glycosylated hemoglobin (HbA₁), glycosylated serum proteins (GSP) and serum lipid profile viz. triacylglycerol, total cholesterol, HDL-cholesterol (HDL-C), VLDL-C, LDL-C and their ratios. HbA₁ and GSP were significantly elevated in diabetic group as a whole when compared with controls (p<0.01), but comparison of mean values of complicated and uncomplicated group revealed (though higher in complicated) that the difference between two groups was not statistically significant (p>0.05). Follow-up studies showed that while GSP levels reflect variations in blood glucose over a shorter period of 15 days, those of HbA₁ do so for about 3 months. HbA₁ correlated with fasting blood glucose (p<0.01), whereas GSP showed correlation with both fasting (p<0.01) and postprandial (p<0.05) blood glucose. Hypercholesterolemia and hypertriacylglycerolemia were observed (p<0.01) in both the groups. However no significant change was seen in HDL-C (p>0.05) even after accounting for sex in both the groups. Interestingly TC:HDL-C was elevated significantly (p<0.01) in the above groups. These findings seem to suggest that at the levels of hyperglycemia, hypercholesterolemia and hypertriacylglycerolemia observed, some patients are prone to develop complications earlier.  相似文献   

Serodiagnosis of tuberculosis using two ELISA systems     

Swati Banerjee  Sonika Gupta  Niraj Shende  Satish Kumar  Bhaskar C. Harinath 《Indian journal of clinical biochemistry : IJCB》2003,18(2):48-53

Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated for detection of mycobacterial antibodies in pulmonary and extra pulmonary tuberculosis. The two test assays explored were ERBA LISA (TB IgG) test (Anda Biologicals) which uses A60 antigen complex found in the cytosol of typical and atypical mycobacteria, and SEVA TB (IgG) ELISA, which uses a 31 kDa, glycoprotein antigen purified fromM. tb H₃₇Ra culture filtrate. Sera from 98 proven tuberculosis [pulmonary TB (48), tuberculous lymphadenopathy (30), tuberculous meningitis (15) & genitourinary TB (5)] were studied along with 32 healthy controls. The overall positivity obtained using ERBA LISA (TB IgG) test and SEVA TB (IgG) ELISA test was 72.9% and 91.6% in pulmonary tuberculosis, 43.3% and 76.6% in tuberculous lymphadenopathy respectively. The sensitivity of ERBA LISA test in tuberculous meningitis and genito-urinary TB was significantly low (26.6% & 40% respectively) compared to sensitivity obtained using SEVA TB ELISA (86.6% & 60% respectively) with overall specificity of 60% and 87.5%. Thus SEVA TB IgG ELISA test was found to be more sensitive than ERBA LISA in detecting IgG antibodies in tuberculous sera, in particular in extra pulmonary tuberculosis cases.  相似文献   

Heterogeneous middle-class and disparate educational advantage: parental investment in their children’s schooling in Dehradun,India     

Achala Gupta 《British Journal of Sociology of Education》2020,41(1):48-63

Abstract

The heterogeneity of the contemporary Indian middle-class has been discussed widely. However, the effect of its internal differences on the distribution of educational resources needs to be examined systematically. Drawing upon in-depth interviews with parents in 53 middle-class families in Dehradun, India, this paper explores three aspects of the home-school relationship: how socioeconomic transformations shape parents’ aspirations for their children’s future, educational decisions parents make to realise those aspirations, and mothers’ engagement in their children’s everyday schooling. The tripartite analysis reveals that despite sharing common educational goals and strategies with the population in general, middle-class families in India use their class privilege to gain valuable educational resources. The paper argues that the discrepancy in the mobilisation of accumulated resources in the heterogeneous middle-class results in disparate educational advantages across families. It critiques the binary construction of social classes when explaining the processes of social reproduction in contemporary Indian society.  相似文献   

Learning Efficiency Versus Low IQ and/or Teachers’ Ratings as Predictors of Reading Ability of ‘Mentally Defective’ Children: a longitudinal study 1     

R. M. Gupta 《Educational studies》1985,11(2):109-118

The major thrust of this study was to test the hypothesis that some of the conventional assessment procedures are not necessarily valid indexes of learning efficiency of children whose tested abilities fall in the ‘mentally defective’ range. The sample of the longitudinal study consisted of 27 second year Junior children, of which six were English and twenty one Asian children. All these children were given the Coloured Progressive Matrices, Draw‐a‐Man test, Schonell Graded Word Recognition Test and the author designed Learning Efficiency Test Battery. The results quite clearly suggest that the information about these children's learning efficiency as measured by the Learning Efficiency Test Battery is more useful for predicting reading and definitely better for predicting changes in reading levels over a period of time than the conventinal type of assessment. It is concluded that the low IQ, should not be deemed as an index of poor learning ability.

  相似文献   

Teaching-for-Learning (TFL): A Model for Faculty to Advance Student Learning     

Clifton F. Conrad  Jason Johnson  Divya Malik Gupta 《Innovative Higher Education》2007,32(3):153-165

In light of the widespread recognition of the enduring challenge of enhancing the learning of all students—including a growing number of students representing diverse racial, ethnic, and socioeconomic backgrounds—there has been an explosion of literature on teaching, learning, and assessment in higher education. Notwithstanding scores of promising new ideas, individual faculty in higher education need a dynamic and inclusive model to help them engage in a systematic and continuous process of exploring and testing various teaching and assessment practices to ensure the learning of their students. This paper introduces a model—Teaching-for-Learning (TFL)—developed to meet this need. Clifton F. Conrad received his bachelor’s degree in History and his master’s degree in Political Science from the University of Kansas and his Ph.D. in Higher Education from the University of Michigan. He is Professor of Higher Education at the University of Wisconsin-Madison; and his research focus is on college and university curricula with particular emphases on program quality, liberal education, and teaching and learning. Jason Johnson received his bachelor’s degree in Comparative History of Ideas and his master’s degree in Educational Leadership and Policy Studies at the University of Washington. He is nearing completion of his Ph.D. and working as a Teaching Assistant in Higher Education at the University of Wisconsin-Madison, and his research focuses on rhetoric in higher education. Divya Malik Gupta received her bachelor’s and master’s degrees in Human Development and Family Studies from Maharaja Sayajirao University in Gujarat, India. She is currently a Ph.D. student at the University of Wisconsin-Madison.  相似文献   

(2) A note on resource sharing in India     

R. K. Das Gupta 《International Information and Library Review》2013,45(3):291-294

  相似文献   

Metal complex dyes for dye-sensitized solar cells: Recent developments     

N. Sekar  Vishal Y. Gehlot 《Resonance》2010,15(9):819-831

Ruthenium complexes are widely used as sensitizers in Dye-Sensitized Solar Cells. Structural diversities of these complexes, their synthesis, and other properties are discussed in this article.  相似文献   

Technology-supported learning environments in science classrooms in India     

Adit Gupta  Darrell Fisher 《Learning Environments Research》2012,15(2):195-216

The adoption of technology has created a major impact in the field of education at all levels. Technology-supported classroom learning environments, involving modern information and communication technologies, are also entering the Indian educational system in general and the schools in Jammu region (Jammu & Kashmir State, India) in particular. This study, which is the first of its kind in India, reports the use of a modified form of Technology-Rich Outcomes-Focused Learning Environment Inventory (TROFLEI) for assessing students?? perceptions of their learning environments in technology-supported science classrooms. Analysis of data from 705 students from 15 classes provided evidence for the reliability and validity of the questionnaire in Indian science classroom settings. The same data also were used for studying gender differences and associations between students?? perceptions of their technology-supported learning environments and three learner outcomes (attitude towards science, academic efficacy and academic achievement).  相似文献