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11.
Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow 总被引:1,自引:0,他引:1
Alessandro Polini Elisa Mele Anna Giovanna Sciancalepore Salvatore Girardo Adriana Biasco Andrea Camposeo Roberto Cingolani David A. Weitz Dario Pisignano 《Biomicrofluidics》2010,4(3)
Producing polymeric or hybrid microfluidic devices operating at high temperatures with reduced or no water evaporation is a challenge for many on-chip applications including polymerase chain reaction (PCR). We study sample evaporation in polymeric and hybrid devices, realized by glass microchannels for avoiding water diffusion toward the elastomer used for chip fabrication. The method dramatically reduces water evaporation in PCR devices that are found to exhibit optimal stability and effective operation under oscillating-flow. This approach maintains the flexibility, ease of fabrication, and low cost of disposable chips, and can be extended to other high-temperature microfluidic biochemical reactors. 相似文献
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Giovanna F. Miranda Francesca Gualtieri Paolo Coccia 《Medical reference services quarterly》2013,32(2):132-145
The new Web generations are influencing the minds and changing the habits of software developers and end users. Users, librarians, and information services professionals can interact more efficiently, creating additional information and content and generating knowledge. This new scenario is also changing the behavior of information providers and users in health sciences libraries. This article reviews the new Web environments and tools that give librarians opportunities to tailor their services better, and gives some examples of the advantages and disadvantages for them and their users. Librarians need to adapt to the new mindset of users, linking new technologies, information, and people. 相似文献
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Giovanna Lelli 《Al-Masaq: Islam & the Medieval Mediterranean》2014,26(2):196-215
This article analyses the presence of Neo-Platonic ideas in the poetics of Ibn Khaldūn's (1332–1406). It particularly focuses on the sixth part of the Muqaddima, in which Ibn Khaldūn presents the Arab-Islamic system of knowledge. I argue that Ibn Khaldūn analyses poetry in terms of a peculiar kind of knowledge and that his views on poetry are largely dominated by a Neo-Platonic paradigm, deriving from Avicenna's Psychology and Sufism. I focus on four topics: the “weak” rational position of poetics among the sciences of logic; the rhetorical norm of mu?ābaqa (“conformity” between “words” and “ideas”, and between “speech” and the “requirement of the situation”), the musical norm of talā?um (appropriateness of note combinations) and the notion of poetical “models” (uslūb; pl. asālīb). My conclusion is that the Muqaddima provides the modern reader with a precious longue-durée overall view of pre-modern Arab-Islamic poetics. 相似文献
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Although not much has been written about older adults' use of academic libraries, they experience challenges navigating these considerable physical and online library spaces. Members of a university lifelong learning community were recruited for a 10-week diary study. They were asked to record their real-time information seeking behaviors related to their study groups, and the challenges they faced looking for or using information. They were also asked to rate their enjoyment to determine whether the joy of engaging in community learning extends to the information seeking process. Findings from sixteen diarists show that they sought information primarily on a specific topic, person, or book. They used a variety of resources but found books and periodicals most useful. They struggled with trust, technology, time management, library anxiety, information overload and dissemination. With increased understanding, librarians can be better equipped to reach older adults that are doing scholarly research and contribute to their success. 相似文献
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Shanyan Lin Sofia Mastrokoukou Claudio Longobardi Paolo Bozzato Francesca Giovanna Maria Gastaldi Martina Berchiatti 《Higher Education Quarterly》2023,77(1):121-137
Transition into higher education (HE) has received increased interest in recent years, since it represents a challenging period for students. The aim of this study was to further understand the associations between self-efficacy, academic achievements, and regulation in first-year university students during their transition into HE. The convenience sample consisted of 374 first-year university students (230 females, 61.5%), aged from 18 to 33 (M = 19.86, SD = 1.51) and recruited from an Italian university. Self-efficacy was assessed using the Motivated Strategies for Learning Questionnaire; regulation strategies were assessed with the Inventory of Learning Patterns of Students; and a self-reported grade point average was taken as an indicator of each student's academic performance. Result shows that students' self-efficacy was positively associated with self-regulation and negatively associated with a lack of regulation. Students with higher self-efficacy and self-regulation strategies had better academic performance. Female students performed better in academic activities and adopted more external regulation strategies. The findings represent an opportunity for university institutions to consider the interventions they provide to first-year students in order to facilitate the successful transition from secondary school; they also provide researchers with further knowledge about the effect of self-efficacy, and regulation strategies on students' adjustment to HE. 相似文献
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Three experiments are reported assessing whether rats prefer controllable over uncontrollable aversive shock. In Experiment 1, subjects chose between escapable and inescapable shock while relative shock duration varied parametrically. In Experiment 2, subjects again chose between escapable and inescapable shock, but duration was held constant and equal. The final experiment gave subjects a choice between avoidable and unavoidable shock under several signaling conditions. Choice behavior proved sensitive to relative shock duration and to predictability of shock but not to controllability of shock. 相似文献
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Luana Porto Barbosa Luciana Quevedo Giovanna Del Grande da Silva Karen Jansen Ricardo Tavares Pinheiro Jerônimo Branco Diogo Lara Jean Oses Ricardo Azevedo da Silva 《Child abuse & neglect》2014
Suicide is among the main causes of death of people aged between 15 and 44 years old. Childhood trauma is an important risk factor for suicide. Hence, the objective of this study was to verify the relationship between childhood trauma and current suicide risk (suicidal behavior and ideation) in individuals aged 14–35 years, in the city of Pelotas, Brazil. This is a cross-sectional, population-based study. Sample selection was performed by clusters. Suicide risk was evaluated using the Mini International Neuropsychiatric Interview (MINI) and Childhood trauma was assessed with the Childhood Trauma Questionnaire (CTQ). Moreover, the participants responded to a questionnaire concerning socioeconomic status, work, and substance use. The sample was composed of 1,380 individuals. The prevalence of suicide risk was 11.5%. The prevalence figures of childhood trauma were 15.2% (emotional neglect), 13.5% (physical neglect), 7.6% (sexual abuse), 10.1% (physical abuse), and 13.8% (emotional abuse). Suicide risk was associated (p < .001) with gender, work, alcohol abuse, tobacco use, and all types of childhood trauma. The odds of suicide risk were higher in women (OR = 1.8), people who were not currently working (OR = 2.3), individuals who presented alcohol abuse (OR = 2.6), and among tobacco smokers (OR = 3.4). Moreover, suicide risk was increased in all types of trauma: emotional neglect (OR = 3.7), physical neglect (OR = 2.8), sexual abuse (OR = 3.4), physical abuse (OR = 3.1), and emotional abuse (OR = 6.6). Thus, preventing early trauma may reduce suicide risk in young individuals. 相似文献
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