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Shan-zhi Gu Xin-han Zhao Ling-xiao Zhang Li Li Zhi-yu Wang Min Meng Gai-li An 《Journal of Zhejiang University. Science. B》2009,10(3):159-167
Objective: To study the effects of the generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAMNEGFASODN) compound on the expressions of vascular endothelial growth factor (VEGF) and its mRNA of breast cancer cells and on the inhibition of vascular endothelial cells. Methods: We examined the morphology of G4PAMAM/VEGFASODN compound and its pH stability, in vitro transfection efficiency and toxicity, and the expressions of VEGF and its mRNA. Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on vascular endothelial cells. Results: The compound was about 10 nm in diameter and was homogeneously netlike. From pH 5 to 10, it showed quite a buffered ability. The 48-h transfection rate in the charge ratio of 1:40 was 98.76%, significantly higher than that of the liposome group (P<0.05). None of the transfection products showed obvious toxicity on the cells. The expressions of both VEGF protein and its mRNA after G4PAMAM/VEGFASODN transfection decreased markedly. Conclusion: With a low toxicity, high safety, and high transfection rate, G4PAMAMNEGFASODN could be a promising gene vector. Specifically, it inhibits VEGF gene expression efficiently, laying a basis for further in vivo animal studies. 相似文献
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《离骚》中的香花芳草具有多重喻指:"我"之美德修能、质变的人才、贤臣及君王。从修辞层面看,芳草意象与其深层喻指之间是一对多的关系;隐喻和转喻是芳草多重喻指的实现机制。从语义层面分析,芳草之芬芳暗示了其喻指对象之美好;而其短促易逝的特点,则隐含了诗人对时光短暂的深切忧虑。其中后者是潜藏在芳草意象背后最为深刻的语义蕴含。 相似文献
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Nuttikarn Nokkaew Podsawee Mongkolpathumrat Ruttanapong Junsiri Supawit Jindaluang Nichagron Tualamun Niya Manphatthanakan Nareumon Saleesee Marisa Intasang Jantira Sanit Punyanuch Adulyaritthikul Kantapich Kongpol Sarawut Kumphune Nitirut Nernpermpisooth 《Indian journal of clinical biochemistry : IJCB》2021,36(2):228
Microvascular and macrovascular diseases are the main causes of morbidity in type 2 diabetes patients through chronic hyperglycaemic condition via oxidative stress and inflammation. Reactive oxygen species (ROS) activate p38 MAPK phosphorylation and inflammation which enhances protein modification by carbonylation. The use of metformin and a p38 MAPK inhibitor is hypothesised to reduce ROS production and inflammation but effects of metformin and p38 MAPK inhibitor (SB203580) on ROS production and inflammation in vascular type 2 diabetes mellitus non-obese (T2DM) have not been investigated. The Goto-Kakizaki rat T2DM model was divided into three groups as T2DM, T2DM treated with 15 mg/kg bw metformin and T2DM treated with 2 mg/kg bw SB203580 for 4 weeks. Rat aortas were isolated and protein carbonyl (PC) contents were measured by spectrophotometric DNPH assay. Aortic IL-1ß level was determined by ELISA. Results showed that aortic PC contents in the T2DM group were significantly higher than in non-diabetic rats. Treatment with metformin or SB203580 significantly reduced PC contents while only metformin significantly reduced IL-1ß levels. Findings indicated that metformin reduced ROS production and inflammation in diabetic vessels and possibly reduce vascular complications in non-obese T2DM. 相似文献
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目的：评价注射用头孢唑肟钠制剂用药的安全性。方法：按照国家SFDA颁发的《化学药物刺激性、过敏性和溶血性研究技术指导原则》进行了局部血管刺激性、肌肉刺激性、溶血性和全身主动过敏性试验。结果：①注射用头孢唑肟钠耳缘静脉点滴给药浓度为0.04g/ml,给药容积为2ml/kg时,对试验兔血管无刺激性;②注射用头孢唑肟钠肌肉注射给药浓度为0.25g/ml,给药容积为0.5ml/只时,对试验兔肌肉无刺激性;③注射用头孢唑肟钠药物浓度为0.04g/ml,在体外试验条件下无溶血反应及红细胞凝聚反应;④注射用头孢唑肟钠致敏剂量为0.067和0.133g/kg,激发剂量为0.067和0.133g/kg时,对豚鼠无主动全身过敏性反应。结论：注射用头孢唑肟钠试验条件下无血管和肌肉刺激性、无溶血性及过敏性反应。 相似文献
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INTRODUCTIONHypertensionisalwaysaccompaniedbyin creasesinarterywallthickness,mainlycausedbyproliferation ,hypertrophy ,migrationandap optosisofvascularsmoothmusclecells(VSMC) ,andelevatedcontentofconnectivetissue .Thesestructuralchangesinbloodvesselsarekn… 相似文献
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以眼底图像为研究对象,从中获得信息：拱环（黄斑）、灌注区、亮点、血管芽及它们的属性,然后根据这些信息帮助眼科医生做出初步诊断。结合临床实际,对这些关键问题展开深入的研究,并将成果应用于医学临床分析和诊断。 相似文献
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当前,中老年人的健身锻炼方兴未艾,大多数人只根据兴趣、爱好和体力状况选择自身的锻炼方式,而由于缺乏对各种健身项目的了解及科学的指导,不少人不断改换锻炼项目,以至收效甚微,基于此,为更好地指导中老年人进行锻炼,就许多健身项目中,太极拳与健身慢跑对中老年人心血管功能、心肺功能的影响,作一比较. 相似文献
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承德自古以来是少数民族聚积区,它的地名多以动物,植物、山川河流,人物的蒙古语、满语命名。地名的产生和变化反映了少数民点生产、生活、社会的变迁。从地名的变迁可以推知少数民族的历史发展状况。 相似文献