Arachnoid cell involvement in the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage     

Zhao-liang Xin  Xiao-kang Wu  Jian-rong Xu  Xi Li 《Journal of Zhejiang University. Science. B》2010,11(7):516-523

Objective: To assess if arachnoid cells have the capability to present antigen and activate T-lymphocytes after stimulation by bloody cerebrospinal fluid (CSF), and to illuminate the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage (SAH). Methods: Arachnoid cells were cultured, characterized, and examined by immunofluorescence for the basal expression of human leukocyte antigen-DR (HLA-DR). Expression of HLA-DR, after co-culturing arachnoid cells in vitro with bloody CSF, was investigated by immunofluorescence and flow cytometry (FCM). The variation of arachnoid cells' ultrastructure was observed by transmission electron microscope (TEM). Arachnoid cells were co-cultured with peripheral blood mononuclear cells (PBMCs). The content of soluble interleukin-2 receptor (sIL-2r) in culture medium was detected by enzyme-linked immunosorbent assay (ELISA). Results: (1) Arachnoid cells were successfully cultured for many passages. The immunofluorescent staining was positive for HLA-DR in over 95% of the human arachnoid cells. The punctate HLA-DR was distributed in cytoplasm and not in the karyon. (2) After co-culturing arachnoid cells in vitro with bloody CSF, numerous particles with strong fluorescence appeared in the cytoplasm on Day 6. On Day 8, the quantity of particles and fluorescent intensity were maximal. FCM showed that the percentage of HLA-DR expressing cells was (2.5±0.4)% at the first 5 d, increasing to (60.8±3.6)% on Day 7. (3) After co-culturing arachnoid cells in vitro with bloody CSF, many lysosome and secondary lysosome particles were present in the cytoplasm. Hyperplasia of rough endoplasmic reticulum and enlarged cysts were observed, with numerous phagocytizing vesicles also observed at the edge of the arachnoid cells. (4) Arachnoid cells stimulated by bloody CSF were co-cultured in vitro with PBMCs. The content of sIL-2r in the culture medium, having been maintained at around 1.30 ng/ml during the first 3 d, had increased by Day 4. The content of sIL-2r peaked 7.53 ng/ml on Day 7 and then reduced gradually. Conclusions: (1) Basic HLA-DR expression is present in arachnoid cells. (2) After stimulation by bloody CSF, arachnoid cells have the potential to serve as antigen presenting cells (APCs) and the ability to activate T-lymphocytes, indicating that arachnoid cells are involved in the mechanism of coagulation-initiated inflammation in the subarachnoid space after SAH.  相似文献   

白术挥发油抑瘤作用研究进展   总被引：2，自引：0，他引：2  

徐玲玲  张耀斌 《西安文理学院学报》2010,13(1):59-61

白术及其挥发油作用于动物实体瘤后能有效抑制肿瘤生长,这种观点已被近年来的许多实验所证实．论述了白术挥发油的化学成分及其抗肿瘤方面的一些发现,并对近年来认为白术抑瘤几种相关机制的研究进展作了简要综述,最后指出了未来该领域研究的重要发展方向．  相似文献   

Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria   总被引：6，自引：0，他引：6  

Song Y  Li M  Li JC  Wei EQ 《Journal of Zhejiang University. Science. B》2006,7(9):749-756

Background： Edaravone had been validated to effectively protect against ischemic injuries. In this study, we investigated the protective effect of edaravone by observing the effects on anti-apoptosis, regulation of Bcl-2/Bax protein expression and recovering from damage to mitochondria after OGD （oxygen-glucose deprivation）-reperfusion. Methods： Viability of PC 12 cells which were injured at different time of OGD injury, was quantified by measuring MTT （2-（4,5-dimethylthia-zol-2-yl）-2,5-diphenyltetrazolium bromide） staining. In addition, PC 12 cells＇ viability was also quantified after their preincubation in different concentration of edaravone for 30 min followed by （OGD）. Furthermore, apoptotic population of PC 12 cells that reinsulted from OGD-reperfusion with or without preincubation with edaravone was determined by flow cytometer analysis, electron microscope and Hoechst/Pl staining. Finally, change of Bcl-2/Bax protein expression was detected by Western blot. Results： （1） The viability of PC12 cells decreased with time （1-12 h） after OGD. We regarded the model of OGD 2 h, then replacing DMEM （Dulbecco＇s Modified Eagle＇s Medium） for another 24 h as an OGD-reperfusion in this research. Furthermore, most PC 12 cells were in the state of apoptosis after OGD-reperfusion. （2） The viability of PC 12 cells preincubated with edaravone at high concentrations （1, 0.1, 0.01 μmol/L） increased significantly with edaravone protecting PC 12 cells from apoptosis after OGD-reperfusion injury. （3） Furthermore, edaravone attenuates the damage of OGD-reperfusion on mitochondria and regulated Bcl-2/Bax protein imbalance expression after OGD-reperfusion. Conclusion： Neuroprotective effects of edaravone on ischemic or other brain injuries may be partly mediated through inhibition of Bcl-2/Bax apoptotic pathways by recovering from the damage of mitochondria.  相似文献   

Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells     

Pan KY  Shen MP  Ye ZH  Dai XN  Shang SQ 《Journal of Zhejiang University. Science. B》2006,7(10):825-829

Objective： Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods： Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline （group B）, vitamin C （group C） and vitamin E （group D）. Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline （sample B）, vitamin C （sample C） and vitamin E （sample D）. Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results： In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion： Vitamin C can protect vascular endothelial cells from mannitol-induced injury.  相似文献   

退火处理对体异质结有机太阳能电池性能的影响     

木丽萍  郑晓虹 《大理师专学报》2013,(4):37-39

制备了结构为：ITO/PEDOT：PSS/P3HT＋PCBM/LiF/Al的有机太阳能电池,研究退火对电池性能的影响,实验发现：经过60 min 150℃退火处理后,器件开路电压（Vo）c为0.57 V,短路电流密度（Js）c达到6.32 mA.cm-2,填充因子（FF）达到0.55,光电转换效率（ηp）达到2.01%,器件性能明显提高。探讨了退火对电池性能影响的内在原因。  相似文献   

线粒体DNA的分子遗传学研究进展   总被引：5，自引：0，他引：5  

陈青  陈家琦 《天津体育学院学报》1997,12(3):16-20

有关线粒体ＤＮＡ（ｍｔＤＮＡ）的突变与人类疾病和衰老乃至肿瘤的关系日益受到人们的关注，研究表明，ｍｔＤＮＡ突变的积累与氧化损伤是人类线粒体遗传病与各种退行性疾病的分子基础。同时，在运动医学领域，研究人员也逐渐发现ｍｔＤＮＡ的遗传多态性是人类有氧耐力的一个有价值的遗传标记，其可作为不同反应群体的基因标志，成为科学选材的理想指标。  相似文献   

Hedgehog信号通路对成骨细胞分化和骨形成的影响及不同方式运动的调控     

陈祥和  李世昌  严伟良  孙朋  季浏  展志林 《北京体育大学学报》2015,38(11):59-64

摘要：目的：探讨Hedgehog信号通路在调控骨髓间充质干细胞(Bone marrow mesenchymal stem cells, BMSCs)增殖、成骨细胞(Osteoblast,OB)分化和成骨能力、骨形成中的生物学调控作用以及运动训练的影响。方法：48只4周龄C57BL/6雄性小鼠,适应性喂养1周后随机分为：对照组（C组）,游泳组（S组）和下坡跑组（D组）,每组16只。S组和D组分别进行游泳（45 min/d,5 d/周,共8周）和下坡跑（坡度-9°,45 min/d,5 d/周,共8周）训练。最后1次训练结束后,断颈椎处死小鼠,利用RT-PCR法检测骨中相关细胞因子mRNA表达;利用SRB染色检测BMSCs增殖能力;利用ALP染色和Von Kossa染色检测OB分化和矿化能力;利用Hologic Discovery A骨密度仪检测骨密度。结果：与C组相比,S组小鼠骨中Ihh(P＜0.05)和Shh(P＜0.05) mRNA表达均出现显著上调,第2 d(P＜0.05)和第4 d(P＜0.01) 的BMSCs数量、OB ALP活性(P＜0.01)均出现显著升高;与C组相比,D组小鼠骨中Ihh(P＜0.01)、Shh(P＜0.01)、Ptch(P＜0.01)和Smo(P＜0.01) mRNA表达均出现显著上调,第2 d(P＜0.01)和第4 d(P＜0.01) 的BMSCs数量、OB ALP活性(P＜0.01)、OB矿化能力(P＜0.01)和BMD(P＜0.01)均出现显著升高;与S组相比,D组小鼠骨中Ihh(P＜0.05)、Shh(P＜0.05)、Ptch(P＜0.05)和Smo(P＜0.05)mRNA表达均显著上调,第2 d(P＜0.01)和第4 d(P＜0.01) 的BMSCs数量、OB的ALP活性(P＜0.05)、OB矿化能力(P＜0.05)、BMD(P＜0.01)均出现显著升高。结论：运动干预可通过Hedgehog信号通路调控BMSCs增殖和OB分化及成骨能力从而促进骨形成,且下坡跑作用效果优于游泳。  相似文献   

NE对THP-1细胞IL-6 HMGB1和CD137表达的影响     

凌伯勋  陈光军  田英 《岳阳职业技术学院学报》2008,23(3):90-93

目的:探讨去甲肾上腺素对人THP-1细胞CD137、IL-6及HMGB1表达的影响.方法:加入101μmol/L的去甲肾上腺素处理THP-1细胞1小时.运用逆转录多聚酶链反应检测其CD137、IL-6及HMGB1mRNA的表达.结果:10μmol/L的去甲肾上腺素能上调THP-1细胞CD137及IL-6mRNA的表达,与对照组比较差异有显著性(P<0.01);但对HMGB1mRNA的表达没有影响.结论:去甲肾上腺素上调THP-1细胞CD137及IL-6mRNA水平.  相似文献   

亚硝化-反硝化固定化细胞捷径生物脱氮的研究   总被引：1，自引：0，他引：1  

廖雪义  蓝荣 《襄樊学院学报》2008,29(8)

选用聚乙烯醇(PVA)和海藻酸钠(CA)作为载体,添加适量SiO2粉末,将筛选出来的高效亚硝化细菌和反硝化细菌分别制成固定化细胞,联合进行脱氮实验.结果表明:按1:1的比例,亚硝化细菌和反硝化细菌固定化细胞接种量为15%(W/V),在温度为30℃,摇床转数为110rpm的条件下处理合成废水(NH4+-N=150mg/L,碱度(NaHCO3=1700mg/L,pH=8.0),27h,脱氮率为97.53%;处理富营养化水体(NH4+-N=34.26mg/L,pH=6.7),20h,脱氮率达到79.51%.  相似文献   

Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-α release of endothelial cells     

Yi-feng JIANG  Feng-di ZHAO  Xiao-bo LI  Yan-xia NING  Xiu-ling ZHI  Rui-zhe QIAN  Lian-hua YIN 《Journal of Zhejiang University. Science. B》2008,9(8)

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