抗人双特异性磷酸酶DUSP18单克隆抗体的制备及鉴定     

彭建柳  陈小芳 《广东教育学院学报》2008,28(5):73-78

双特异性磷酸酶（Dual Specificity Phosphatase）是酪氨酸磷酸酶家族中的一员,这个家族中的成员既能对磷酸化酪氨酸去磷酸化,也能对磷酸化丝／苏氨酸去磷酸化．此外,它们还在有丝分裂原激活蛋白磷酸酶信号途径（MAPK）中起重要生理功能．为了制备抗人双特异性磷酸酶的单克隆抗体（mAb）,以DUSP18重组蛋白为抗原免疫BALB／e小鼠,通过B淋巴细胞杂交瘤技术制备抗相应磷酸酶的mAb．用Western blot检测mAb对重组蛋白和细胞中天然磷酸酶的反应性,共获得6株可稳定分泌抗磷酸酶mAb的杂交瘤细胞株,为进一步研究双特异性磷酸酶的去磷酸化作用机理以及其在有丝分裂原激活蛋白磷酸酶信号途径（MAPK）中的信号转导提供强有力的工具．  相似文献   

用Auto CAD标注符合国标的标高符号     

欧阳红  柯昌胜 《长江工程职业技术学院学报》2007,24(2):50-51

用Auto CAD来绘制土木工程图,用户必须解决标高符号的标注问题.本文介绍Auto CAD中用带有属性的图块,把标高符号数值定义为可变属性进行插入来标注标高符号的方法,完全符合国家标准.  相似文献   

Evaluation of Serum Vitamin B12 Levels and Its Correlation with Anti-Thyroperoxidase Antibody in Patients with Autoimmune Thyroid Disorders     

S. Jaya Kumari  Ganapathy Bantwal  Anitha Devanath  Vageesh Aiyyar  Madhuri Patil 《Indian journal of clinical biochemistry : IJCB》2015,30(2):217-220

Vitamin B12 deficiency has been reported in patients with Autoimmune thyroid disorders. However there is limited data on exact prevalence of low B12 and its correlation with anti-thyroperoxidase antibody (anti-TPO) levels in these patients. The aim of our study was to estimate serum vitamin B12 levels in autoimmune thyroid disorders and to correlate B12 levels with anti-TPO. 350 patients were selected by convenient sampling. Vitamin B12 levels and thyroid parameters were estimated using fully automated chemiluminescence method on Access 2. Results of our study shows that using the manufacturer’s cut-off of 145 pg/mL, the prevalence of low serum vitamin B12 was found to be 45.50 %. Higher prevalence (55 %) was seen based on the published cut-off of 200 pg/mL The study however did not demonstrate any significant correlation between vitamin B12 levels and anti-TPO (r = −0.11 and p value of 0.30).

Electronic supplementary material

The online version of this article (doi:10.1007/s12291-014-0418-4) contains supplementary material, which is available to authorized users.  相似文献   

英国汽车维修职业教育模式分析与借鉴   总被引：2，自引：1，他引：1  

曾鑫 《黄冈职业技术学院学报》2010,12(2):22-24

本文通过对英国汽车维修专业职业教育的特点进行分析,找出可供我们借鉴和学习的地方,提出目前我们可以进一步开展的交流与合作内容。  相似文献   

“教学工厂式”汽车维修技术实训基地建设的探讨     

李献龙 《大众科技》2012,(8):186-187,183

中职"教学工厂式汽车维修技术实训基地"新模式突破了传统实训基地模式的局限,文章阐述了与该模式相适合的教学环境、培养相适应的教师队伍、开发相适用的项目课程、探索相适当的教学方式以及引进相适宜的校企项目等,为中职学校汽车维修实训基地的建设提供了一种值得借鉴的模式。  相似文献   

Serum thyroglobulin antibody levels within or near to the reference range may interfere with thyroglobulin measurement     

Zoltán L?csei  István Szabolcs  Károly Rácz  Gábor L. Kovács  Dóra Horváth  Erzsébet Toldy 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2012,22(3):365-370

High concentration of thyroglobulin antibodies (TgAb) is a major limiting factor of thyroglobulin measurements in patients with differentiated thyroid cancer. We investigated whether thyroglobulin antibody added to serum samples could interfere with the thyroglobulin assay. Thyroglobulin levels in serum samples with different concentrations of thyroglobulin were measured by electrochemiluminescence immunoassay before and after the addition of increasing concentrations of thyroglobulin antibody using the secondary calibrator solution of the thyroglobulin assay kit containing sheep thyroglobulin antibody to reach thyroglobulin antibody levels within or near to the reference range. Thyroglobulin and thyroglobulin antibody concentrations were also measured in 134 serum samples from 27 patients after thyroid ablation. There was a strong negative association (slope = −1.179) between thyroglobulin antibody and thyroglobulin concentrations in samples with added thyroglobulin antibody (beta = −0.86; P < 0.001). Changes in thyroglobulin concentrations were described mathematically as loss of thyroglobulin% = −0.2408 × Ln(thyroglobulin antibody IU/ml) + 0.1944. Thyroglobulin concentrations were significantly lower than those calculated from experiments with added thyroglobulin antibody in 26/134 samples from patients after thyroid ablation. We conclude that if the same TgAb interference exists in the presence of naturally occurring human TgAb, our observation may prove to be useful during follow-up of patients with differentiated thyroid cancer. However, further studies are needed to explore the clinical relevance of thyroglobulin antibody levels within or near to the reference range in monitoring these patients.  相似文献   

基于反序词典的中文逆向最大匹配分词系统设计*   总被引：6，自引：0，他引：6  

张李义  李亚子 《现代图书情报技术》2006,1(8):42-45

介绍几种常见的分词算法，在改进传统的反序词典、优化逆向最大匹配算法的基础上，设计并实现基于逆向最大匹配的中文分词系统，试验证明速度和精度都有显著提高。  相似文献   

中国股票市场与干散货航运市场的动态相关性——基于DCC-MGARCH和VAR模型的实证分析     

唐韵捷  曲林迟 《上海海事大学学报》2015,36(1):38-45

为探求中国股票市场与干散货航运市场的动态相关性,运用DCC-MGARCH模型和向量自回归(Vector Auto-Regressive,VAR)模型对上证综指和波罗的海干散货指数(Baltic Dry Index,BDI)进行分析,发现这两个市场之间存在信息溢出现象,具有较强的动态相关性.作为重要纽带的上证综指与BDI之间的动态相关性是制定海运运价不可或缺的因素,也可以作为金融资产定价的重要因素.  相似文献   

遥控方便桌的设计     

王清昀 《中国科技纵横》2014,(17):25-26

本遥控方便桌属机械电子类,病人不能下床搬桌,通过遥控装置方便灵活移动与升降桌子。该项目已获实用新型专利证书,专利号：ZL201220436108.1。①该桌配备红外线遥控器控制功能,感应器反应快速。病人操作简单,使用方便。②桌面电动升降,安全可靠。根据病人医治、看书和上网所需,手动或自动遥控桌子进退,左右转及桌面升降。③利用单片机根据程序使桌子有自动行进,病人只按遥控器中一个按键,桌子即自动进退转弯和停止。④轻巧牢固,节约空间,方便携带,可叠放。桌子整体重约5kg。  相似文献   

自动检测系统中的接地抗干扰技术     

刘兆平 《九江职业技术学院学报》2006,(2):23-24

为了消除自动检测系统中的一些无用信号，需采取相应的措施抑制干扰。本文主要介绍抑制干扰的最常用的、行之有效的接地抗干扰技术。  相似文献