＂柯氏模式＂在教学评价中的运用     

时广军 《番禺职业技术学院学报》2014,(3):15-20

教学评价是教育与培训领域的一个重要环节。在借鉴以往教学评价模式的基础上,“柯氏模式”从“体验一所获一运用一产出”的思路出发,由外到内,渐渐深入,综合多方面的意见,是一种更为系统的评价模式。该模式认为要树立高级到低级的评估理念、建立信息资料库,加强各方合作、重视行为评价、形成“一体化”的评价流程,能够对当今的教学评价起到一定的启示作用。  相似文献   

Gay–straight alliance (GSA) members’ engagement with sex education in Canadian high schools     

Alicia Lapointe 《Sex education》2014,14(6):707-717

This paper offers an examination of gay–straight alliance (GSA) members’ engagement with sex education, sexual health, and prejudice and discrimination in Canadian public high schools. It explores how five students’ (four straight and one gay-identifying) participation in GSAs served as a springboard for learning about and challenging stereotypes; prejudice; and discrimination directed at lesbian, gay, bisexual, trans, and queer/questioning (LGBTQ) people. Queer theory provided the theoretical underpinnings of the study, offering a lens through which to examine the heteronormative underpinnings of education, and a means to interpret how homophobic discourses circulate in school and society. Empirical data were obtained via observational notes from visits to nine GSAs and semi-structured interviews with the five GSA members. Findings suggest that straight allies can use their heterosexual privilege to address LGBTQ issues with their peers. Through GSA involvement, participants learned to interrogate and combat stereotypes about LGBTQ people and HIV-related myths, as well as to engage in queer discussion and political action.  相似文献   

Deregulation of microRNA expression in thyroid tumors简     

Zi-ming YUAN  Zhi-li YANG  Qi ZHENG 《Journal of Zhejiang University. Science. B》2014,15(3):212-224

MicroRNAs (miRNAs or miRs) are endogenous non-coding RNAs that negatively regulate gene expression by binding to the 3′ non-coding regions of target mRNAs, resulting in their cleavage or blocking their translation. miRNAs may have an impact on cell differentiation, proliferation, and survival, and their deregulation can be inclined to diseases and cancers, including thyroid tumors. The purpose of this review is to summarize the existing findings of deregulated miRNAs in different types of thyroid tumors and to exhibit their potential target genes, especially to demonstrate those involved in tumor invasion and metastasis. In addition, new findings of circulating miRNA expression profiles, single nucleotide polymorphism (SNP) in thyroid tumors, and the correlation of somatic mutations with deregulated miRNA expression in thyroid tumors were all included in this review.  相似文献   

Influence of casein phosphopeptide-amorphous calcium phosphate application,smear layer removal,and storage time on resin-dentin bonding     

Jun Lin  Wei-ying Zheng  Peng-ruo-feng Liu  Ning Zhang  Hui-ping Lin  Yi-jing Fan  Xin-hua Gu  Oliver Vollrath  Christian Mehl 《Journal of Zhejiang University. Science. B》2014,15(7):649-660

The aim of this study is to evaluate the influence of Tooth Mousse (TM) application, smear layer removal, and storage time on resin-dentin microtensile bond strength (μTBS). Dentin specimens were divided into two groups: (1) smear layer covered; (2) smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive (Clearfil SE Bond (CSE); Kuraray Medical, Tokyo, Japan), an all-in-one adhesive (G-Bond (GB); GC Corp, Tokyo, Japan), and a total-etch adhesive (Adper Single Bond 2 (SB); 3M ESPE, St. Paul, MN, USA), the specimens were stored for 3 d or 6 months in deionized water at 37 °C, and μTBS was tested and analyzed. With the exception of SB (no TM application) and GB, the μTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage (P≤0.001). Bond strength of GB decreased significantly when using EDTA (3 d storage, P<0.05). TM application only increased the μTBS of GB (no EDTA) and SB (with EDTA) after 3 d (P≤0.02). Comparing the adhesives after 3 d of storage, CSE exhibited the greatest μTBS values followed by GB and SB (P≤0.02). The factors of adhesive, EDTA, and TM did not show any significant impact on μTBS when specimens were stored for 6 months (P>0.05). The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on μTBS of dentin bonds after a period of 6 months.  相似文献   

The polyadenylation code: a unified model for the regulation of mRNA alternative polyadenylation   总被引：3，自引：2，他引：1  

Ryan DAVIS  Yongsheng SHI 《Journal of Zhejiang University. Science. B》2014,15(5):429-437

The majority of eukaryotic genes produce multiple mRNA isoforms with distinct 3′ ends through a process called mRNA alternative polyadenylation (APA). Recent studies have demonstrated that APA is dynamically regulated during development and in response to environmental stimuli. A number of mechanisms have been described for APA regulation. In this review, we attempt to integrate all the known mechanisms into a unified model. This model not only explains most of previous results, but also provides testable predictions that will improve our understanding of the mechanistic details of APA regulation. Finally, we briefly discuss the known and putative functions of APA regulation.  相似文献   

非参数项目反应理论在小规模测验中的运用     

张军 《考试研究》2014,(1):56-61

单调匀质模型是非参数项目反应理论中使用最广泛的模型,它有三个基本假设,适用于小规模测验的分析。本研究使用MHM分析北京语言大学汉语进修学院某次测验,结果表明测验满足弱单维性假设与弱局部独立性假设,67个项目中有9个项目的量表适宜性系数低于0.3,需要修改或删除,删除后测验为中等强度的Mokken量表。另外,有2个项目违反了单调性假设,不符合Mokken量表的要求。  相似文献   

辅助生殖技术学龄前单双胎子代的认知发展存在差异么?(英文)简   总被引：1，自引：0，他引：1  

Lan-feng XING  Yu-li QIAN  Lu-ting CHEN  Fan-hong ZHANG  Xin-fen XU  Fan QU  Yi-min ZHU 《Journal of Zhejiang University. Science. B》2014,15(1):51-57

Objective： To explore whether there exist differences in cognitive development between singletons and twins born after in vitro fertilization （IVF） or intracytoplasmic sperm injection （ICSI）. Methods： A total of 566 children were recruited for the study, including 388 children （singletons, n=175; twins, n=213） born after IVF and 178 children （singletons, n=87; twins, n=91 ） born after ICSI. The cognitive development was assessed using the Chinese-Wechsler Intelligence Scale for Children （C-WISC）. Results： For all pre-term offspring, all the intelligence quotient （IQ） items between singletons and twins showed no significant differences no matter if they were born after IVF or ICSI. There was a significant difference in the cognitive development of IVF-conceived full-term singletons and twins. The twins born after IVF obtained significantly lower scores than the singletons in verbal IQ （containing information, picture ＆ vocabulary, arithmetic, picture completion, comprehension, and language）, performance IQ （containing maze, visual analysis, object assembly, and performance）, and full scale IQ （P〈0.05）. The cognitive development of full-term sin- gletons and twins born after ICSI did not show any significant differences. There was no significant difference between the parents of the singletons and twins in their characteristics where data were collected, including the age of the mothers, the current employment status, the educational backgrounds, and areas of residence. There were also no consistent differences in the duration of pregnancy, sex composition of the children, age, and height between sin- gletons and twins at the time of our study although there existed significant differences between the two groups in the sex composition of the full-term children born after ICSI （P〈0.05）. Conclusions： Compared to the full-term singletons born after IVF, the full-term twins have lower cognitive development. The cognitive development of full-term singletons and twins born after ICSI did not show any significant differences. For all pre-term offspring, singletons and twins born after IVF or ICSI, the results of the cognitive development showed no significant differences.  相似文献   

In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel oftea (Camellia sinensis L.)简   总被引：1，自引：0，他引：1  

Yue-fei Wang  Jie Wang  Jing Wu  Ping Xu  Yi-qi Wang  Jun-jie Gao  Danielle Hochstetter 《Journal of Zhejiang University. Science. B》2014,15(2):173-180

The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied. Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM). Under the optimized conditions, the yield of TFPP was predicted to be 4.98%. The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated. We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions. They had not only remarkable scavenging activity on 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro. Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.  相似文献   

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp简     

Tossapon Wongtangprasert  Wirongrong Natakuathung  Umaporn Pimpitak  Anumart Buakeaw  Tanapat Palaga  Kittinan Komolpis  Nanthika Khongchareonporn 《Journal of Zhejiang University. Science. B》2014,15(2):165-172

A novel monoclonal antibody (MAb) against oxytetracycline (OTC) was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay (ELISA)-based detection system. An OTC-bovine serum albumin (BSA) conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2–4F, 7–3G, and 11–11A. An indirect competitive ELISA (icELISA) was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration (IC50) and percentage of cross-reactivity, respectively. MAb 2–4F exhibited the highest sensitivity, with an IC₅₀ of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no cross-reactivity to other unrelated antibiotics. When MAb 2–4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%–118% for an intra-assay and 96%–113% for an inter-assay. The coefficients of variation of the assays were 3.9%–13.9% and 5.5%–14.9%, respectively.  相似文献   

Proteins induced by telomere dysfunction are associated with human IgA nephropathy     

Ying-ying LU  Xian YANG  Wen-qing CHEN  Zhen-yu JU  Zhang-fei SHOU  Juan JIN  Xiao-hui ZHANG  Jiang-hua CHEN  Hong JIANG 《Journal of Zhejiang University. Science. B》2014,15(6):566-574

Aging is one of the contributing risk factors for kidney diseases. Accumulating evidence prompts the view that telomere length in kidney tissue cells is an indicator for organismal aging. Previously identified aging markers (cathelin-related antimicrobial peptide (CRAMP), stathmin, elongation factor-1α (EF-1α), and chitinase) were associated not only with telomere driven aging in mice but also with human aging and chronic diseases. This study focuses on the relationship between these biomarkers and IgA nephropathy (IgAN) progression in the Chinese population. For 260 individuals, the four markers are determined in blind datasets using direct enzyme-linked immunosorbent assay (ELISA) and immunofluorescence staining. The expression levels of CRAMP and chitinase increased in blood plasma, urine, and kidney tissues during human IgAN progression. And for the other nephropathy, such as systemic lupus erythematosus (SLE), diabetic nephropathy (DN), and focal segmental glomerulosclerosis (FSGS), there is no protein upregulation with telomere shortening. Moreover, a combination of CRAMP and chitinase can distinguish patients with IgAN from healthy individuals with 88.2%/92.5% (plasma) and 74.3%/84.2% (urine) sensitivity/specificity. These data provide the experimental evidence that telomere shortening and related inflammatory proteins are associated with human IgAN, and it could be a new direction for the disease progression study.  相似文献