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Yan-xia Ning Shun-lin Ren Feng-di Zhao Lian-hua Yin 《Journal of Zhejiang University. Science. B》2010,11(5):350-356
Objective:To determine the effect of steroidogenic acute regulatory protein(StAR) overexpression on the levels of adenosine triphosphate(ATP)-binding cassette transporter A1(ABCA1) and ATP-binding cassette transporter G1(ABCG1) in an endothelial cell line(bEnd.3).Methods:The StAR gene was induced in bEnd.3 cells with adenovirus infection.The infection efficiency was detected by fluorescence activated cell sorter(FACS) and fluorescence microscopy.The expressions of StAR gene and protein levels were detected ... 相似文献
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摘要：目的：探讨葡萄糖转运蛋白1（Glucose Transporter 1, GLUT1）基因rs841853 G/T多态位点作为评估和筛选长跑运动能力遗传标记的可行性。方法：于2009-2010年选取78名中国女子专业长跑运动员,采用基质辅助激光解吸附电离飞行时间质谱检测技术解析GLUT1基因rs841853位点基因型,采用递增负荷跑台运动试验获取运动员的生理机能指标,并检索追溯运动员截止到2017年各主项的个人最好成绩。结果：中国女子专业长跑运动员rs841853 G/T多态位点的基因型GG、GT和TT的频率分布为65.4%、29.5%和5.1%,等位基因G和T的频率分布为80.1%和19.9%,符合哈温平衡定律。基因型频率和等位基因频率与北京汉族人群存在显著差异（P<0.05,P<0.05）。GT+TT基因型携带者的VO2AT/VO2max、VE和5000米项目个人最好成绩显著高于GG基因型携带者（P<0.05,P<0.05,P<0.05）。结论：GLUT1基因rs841853位点的T等位基因可能是评估优秀长跑运动能力的基因标记,有待于通过扩大样本量和功能学研究给予进一步验证。 相似文献
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观察分析在不同的运动强度诱导的"运动性酸中毒"环境中,大鼠肾小管上皮细胞刷状缘侧膜钠离子依赖性枸橼酸转运蛋白mRNA表达量的变化.结果表明:不同运动强度对枸橼酸转蛋白mRNA表达量的作用不一.A组运动强度较小,血乳酸的水平相对于对照组无显著性升高(P>0.05),转运蛋白mRNA表达量也无显著性差异(P>0.05).B组和C组运动强度较大,血乳酸水平相对于对照组显著性升高(P>0.05),转运蛋白mRNA表达量也呈现明显增加(P<0.05).C组较B组血乳酸水平显著性升高(P<0.05),但两组转运蛋白mRNA表达量却无显著性差异(P>0.05),提示可能存在酸碱度变化对转运蛋白mRNA表达量影响作用的阈值. 相似文献
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尤超尘 《山西师大体育学院学报》2002,17(3):66-67
葡萄糖载体（GLUT）是细胞膜上介导葡萄糖跨膜转运的蛋白质，有6种不同的亚型。骨骼肌中存在3种，即GLUT1、GLUT4和GLUT5.它们对骨骼肌的葡萄糖利用有重要意义。运动能促进GLUT的表达及合成，从而增强肌肉利用葡萄糖的能力。 相似文献
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硫酸氢钠催化合成柠檬酸三丁酯 总被引：2，自引：0，他引：2
以柠檬酸、正丁醇为原料，硫酸氢钠为催化剂，催化合成柠檬酸三丁酯．考察了影响产率的多种因素．确定了最佳反应条件． 相似文献
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Santi Esteva Pere Panisello Joan Ramon Torrella Teresa Pagés 《Journal of sports sciences》2013,31(6):633-640
Abstract We studied the effect of intermittent hypobaric hypoxia exposure on lactate dehydrogenase and citrate synthase activities, together with myoglobin content, of rat myocardium, tibialis anterior, and diaphragm muscles. The intermittent hypoxia exposure programme consisted of daily 4-h sessions in a hypobaric chamber (5000 m) over a period of 22 days. Samples were taken at the end of the programme, and 20 and 40 days later, and compared with those of control animals. In myocardium, lactate dehydrogenase activity was significantly depressed in animals 20 days post-exposure (314.6 ± 15.3 IU · g?1) compared with control animals (400 ± 14.3 IU · g?1), while citrate synthase activity and myoglobin concentration showed a significant stepwise increase from control animals (88.2 ± 3.6 IU · g?1 and 4.38 ± 0.13 μm · mg?1) to animals 20 days (104.7 ± 3.7 IU · g?1 and 5.01 ± 0.17 μm · mg?1) and 40 days post-exposure (108.8 ± 6.5 IU · g?1 and 5.11 ± 0.22 μm · mg?1). In contrast, no differences were found in diaphragm and tibialis anterior muscles. Our results show that intermittent hypobaric hypoxia exposure increased the oxidative character of myocardium even 20 days after the hypoxic stimulus has ceased, and that this effect lasts for more than 40 days for citrate synthase activity and myoglobin concentration. These findings support our previous results on skeletal and cardiac muscle capillarization after passive intermittent simulated altitude exposure, thus providing morphofunctional and biochemical evidence for increased cardiac aerobic efficiency. 相似文献