Kinetic analysis of γ-glutamyltransferase reaction process for measuring activity via an integration strategy at low concentrations of γ-glutamyl p-nitroaniline     

Li ZR  Liu Y  Yang XL  Pu J  Liu BZ  Yuan YH  Xie YL  Liao F 《Journal of Zhejiang University. Science. B》2011,12(3):180-188

At 0.12 mmol/L γ-glutamyl p-nitroaniline (GGPNA), an improved integrated method was developed for kinetic analysis of γ-glutamyltransferase (GGT) reaction process and the integration with the classical initial rate method to measure serum GGT. For the improved integrated method, an integrated rate equation, which used the predictor variable of reaction time and considered inhibitions by both GGPNA and products, was nonlinearly fit to GGT reaction processes. For the integration strategy, classical initial rates were estimated when GGPNA consumption percentages were below 50%; otherwise, maximal reaction rates of GGT were estimated by the improved integrated method and converted into initial rates according to the differential rate equation at 0.11 mmol/L GGPNA. The integration strategy was validated using optimized GGT kinetic parameters and 10-s intervals to record reaction curves within 8.0 min. By the integration strategy, there was a linear response from 0.9 to 32.0 U/L GGT, coefficients of variation were below 3.5% for GGT from 8.0 to 32.0 U/L (n=5), and GGT activities in clinical sera responded linearly to their classical initial rates at 2.00 mmol/L GGPNA with an expected slope. Therefore, the integration strategy was successful in measuring GGT at 0.12 mmol/L GGPNA.  相似文献   

以情动人——谈版式设计中情感的传达     

刘晶晶 《河南职业技术师范学院学报(职业教育版)》2011,(7)

现代版式设计强调创意、突出个性、注重情感,具有情感化的版面在现代版式设计中越来越重要。文章探讨了如何使版面具有丰富的情感以及在版面中更好传达情感的编排方式。  相似文献   

现代汉语兼语结构的机器探测     

傅成宏 《合肥教育学院学报》2011,(6):52-56

采用统计与规则结合的方法对现代汉语兼语结构的机器探测方法展开研究,提出了用于自动探测兼语的＂兼语候选项特征＂与＂支持度＂的概念,同时设计出了相应的算法,并在此基础上,用Visual C＋＋编译出了相应的＂现代汉语兼语结构机器探测软件＂。通过对200万字的《人民日报》1998年1月语料进行机器探测试验和测试,发现：对于现代汉语兼语结构的探测,兼语候选项特征与支持度的计算起到了相互补充的作用。实验结果显示,探测达到80%左右的调和平均值,基本达到实用水平。  相似文献   

基于Masterean凹、凸曲面加工方式应用研究     

修辉平  吴金会 《九江职业技术学院学报》2011,(3):17-19,52

本文基于Mastercam曲面加工的特点,研究了凹、凸形曲面平行铣削、放射状、等高外形等典型的粗加工和精加工的加工方式,比较它们的加工路径、加工质量及加工效率,得出了高效、优质、省时的曲面加工方法。同时为其它自动编程软件的曲面加工的高效加工研究提供了借鉴。  相似文献   

“钢中锰含量的测定”实验的数据处理方法     

任庆云  王松涛  马丽娜  李文娟 《集宁师专学报》2011,33(4):38-41

本文采用Visual Basic 6.0语言结合最小二乘法直线拟合的方法开发了＂钢中锰含量的测定＂实验的数据处理软件,能够得到科学与准确的数据处理结果,并能够打印出图形。  相似文献   

青花瓷工艺     

陈其富 《闽江学院学报》2011,32(2):137-140

通过介绍青花瓷的历史成就与影响,揭示了青花瓷制作工艺的重要性,因此重点介绍青花瓷制作工艺的整个流程.其流程由青花瓷瓷土加工工艺—青花瓷制坯工艺—青花瓷的釉与料工艺—青花瓷装饰工艺—青花瓷烧成工艺等5个部分组成.  相似文献   

民族地区畜产品加工学实验教学中的问题与改革探索     

杨万根  陈雪花 《科教文汇》2020,(12):84-85,94

畜产品加工学是食品科学与工程类本科专业的选修课程之一,由理论课和实验课两部分构成,具有很强的应用性和实践性。本文以吉首大学为例,分析了民族地区高校在畜产品加工学实验教学中遇到的问题,并结合湘西地区畜产品加工产业情况对该课程实验教学部分进行了改革和调整,实现了丰富畜产品加工学实验教学内容、创新实践教学方式、培养高质量专业技术型人才的目标。  相似文献   

创业者社会网络、市场信息对创业绩效的影响研究     

赵文红  孙万清  王垚 《科学学研究》2013,31(8):1216-1223

 摘要： 本文探讨了社会网络和市场信息对创业企业绩效的影响,并以西安高新技术产业开发区154家创业企业的调研数据为依托,实证检验了个人网络和商业网络在这个过程中所起到的作用。研究结果显示,个人网络与商业网络对市场信息获取与利用的过程具有不同的作用,对于市场信息的获取,商业网络具有更加积极的作用,而对于市场信息的利用,个人网络则具有更加积极的作用。市场信息的获取正向影响市场信息的利用,而市场信息的利用对新创企业绩效有显著的正向影响。这些结论为创业实践中对于个人网络和商业网络作用的认识,以及市场信息获取与利用的关系提供了相应的管理启示。  相似文献   

基于图像处理的眼球自动对焦系统研究     

王萌  陈鸿 《中国科技信息》2013,(7):46-47

对眼球中心瞳孔清晰对焦是验光仪等现代眼科医疗仪器正常工作的重要前提,提出一种基于图像处理的眼球自动对焦法。该方法是对传统的登山随动聚焦法的改进,巧妙利用光斑,引入图像清晰度评价参数H,在搜索过程中先大步距缩小物距,然后计算出H后进行小步距精确对焦。实验表明：本方法对焦系统具备良好的准确性、实时性、抗干扰性和较强的鲁棒性,达到了理想的对焦效果。  相似文献   

Body learning: examining the processes of skill learning in dance     

Richard Bailey  Angela Pickard 《Sport, Education and Society》2013,18(3):367-382

This paper was stimulated by the authors' attempt to understand the process of skill learning in dance. Its stimulus was a period of fieldwork based at the Royal Ballet School in London, and subsequent discussions with the school's teachers and with academic colleagues about how it was that the young dancers developed their characteristic set of skills. The paper's focus and method is philosophical, although it draws on empirical data (albeit sparingly) where it is relevant to the discussion.

Many accounts of skill learning are premised on the presumption that learners come to know about their environment by representing it in the mind and that such representation is a result of a computational process involving information received through the senses. This is the dominant position of researchers in the field. In this paper, we discuss weaknesses in this information processing theory, and explore the elements of a more satisfactory approach. The account offered here locates dance skills within the context of development, in which skills are incorporated in learners through practice and training in dance environments. Dreyfus' theory of expertise and Bourdieu's theory of practice offer insights into the practical mastery young dancers carry in their bodies, which cannot be accurately formulated in terms of a system of representations. Dancers, from this perspective, do not passively follow instructions, but actively engage in tasks involving characteristic ways of moving that are situated in the nexus of relations between people and the distinctive contexts of dance. The paper suggests that skill learning in dance (or indeed any domain) is not a matter of processing information, but is imminent in the active, perceptual engagement of learner and context.  相似文献