排序方式： 共有111条查询结果，搜索用时 31 毫秒
11.
目的：探讨转化生长因子β1（TGF-β1）、半胱氨酸天冬氨酸蛋白酶3（Caspase-3）及肾母细胞瘤抑癌基因蛋白（WT1）在肾活检肾小球肾炎组织中的表达及其意义。方法：采用Envision法对40例肾小球肾炎组织及7例对照组肾组织进行TGF-β1、Caspase-3和WT1免疫组织化学染色标记、分析。结果：TGF-β1、Caspase-3、WT1在各种肾炎组织中均有表达,各实验组与对照组差异有统计学意义（P〈0.05）。TGF-β1在系膜增生性肾小球肾炎组的表达明显低于膜性肾病组（t=-4.131,P=0.002）;Caspase-3在各组别中其表达差异无统计学意义;WT1在大量高蛋白尿组表达低于非大量蛋白尿组（t=-2.130,P=0.046）;其余组别差异无统计学意义。WT1的低表达与TGF-β1、Caspase-3的高表达相关。结论：TGF-β1、Caspase-3表达的上调及WT1表达的下调与肾病理改变密切相关,蛋白尿和足细胞减少可能是肾脏病进展的预测因子及肾小球硬化的原因。 相似文献
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文章研究旨在优化前期筛选到的米曲霉HML366产β-葡萄糖苷酶的培养基条件。通过单因素实验和响应面设计对米曲霉HML366产β-葡萄糖苷酶的培养基条件进行了优化组合,确定最佳的发酵培养基配方为:甘蔗渣3.49%,豆饼粉0.375%,KH2PO4 0.255%。在此培养条件下得到β-葡萄糖苷酶酶活力达92.64 U/g,是未优化前酶活71.12 U/g的1.30倍。 相似文献
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Cristina Tringali 《Journal of sports sciences》2013,31(5):452-461
AbstractExercise performed at a competitive level could deeply modify the immune system and the cytokine response of athletes. In this report, we demonstrated that young elite female artistic gymnasts (n = 16; age: 9–15 years) showed an increase of interleukin 6 (IL-6) and tumour necrosis factor alpha (TNF-α) mRNA expression in blood mononuclear cells (PBMCs), in comparison to girls performing the same sport at a recreational level (n = 16; age: 10–15 years). The increase of IL-6 and TNF-α mRNAs appeared to be directly linked to the intensity and duration of the training. Moreover, in elite athletes engaged in artistic gymnastics or in synchronised swimming (n =34; age: 9–15 years), IL-6 gene expression appeared to be modulated by the levels of circulating oestrogens: pre-pubertal athletes (n = 20; age: 11 ± 1 years) revealed a higher increase in IL-6 than pubertal athletes (n = 14; age: 14 ± 1.6 years). In pre-pubertal athletes, body mass index (BMI) percentile was inversely correlated with the increase of both IL-6 and TNF-α. The consequence of these events was the shift of the cytokine profile towards a pro-inflammatory status. These modifications, induced by training performed at an elite level, might negatively affect the growth of female children athletes. 相似文献
14.
Building muscle: nutrition to maximize bulk and strength adaptations to resistance exercise training
《European Journal of Sport Science》2013,13(2):67-76
Abstract Several nutritional strategies can optimize muscle bulk and strength adaptations and enhance recovery from heavy training sessions. Adequate energy intake to meet the needs of training and carbohydrate intake sufficient to maintain glycogen stores (>7 g carbohydrate·kg?1·day?1 for women; >8 g carbohydrate·kg?1·day?1 for men) are important. Dietary protein intake for top sport athletes should include some foods with high biological value, with a maximum requirement of approximately 1.7 g·kg?1·day?1 being easily met with an energy sufficient diet. The early provision of carbohydrate (>1 g·kg?1) and protein (>10 g) early after an exercise session will enhance protein balance and optimize glycogen repletion. Creatine monohydrate supplementation over several days increases body mass through water retention and can increase high-intensity repetitive ergometer performance. Creatine supplementation can enhance total body and lean fat free mass gains during resistance exercise training; however, strength gains do not appear to be enhanced versus an optimal nutritional strategy (immediate post-exercise protein and carbohydrate). Some studies have suggested that β-OH-methyl butyric acid (β-HMB) can enhance gains made through resistance exercise training; however, it has not been compared “head to head” with optimal nutritional practices. Overall, the most effective way to increase strength and bulk is to perform sport-specific resistance exercise training with the provision of adequate energy, carbohydrate, and protein. Creatine monohydrate and β-HMB supplementation may enhance the strength gains made through training by a small margin but the trade-off is likely to be greater bulk, which may be ergolytic for any athlete participating in a weight-supported activity. 相似文献
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以L-丝氨酸为手性源,经过酯化,缩合,还原氨化,关环,格氏反应,成功地得到了一种新的手性β-氨基醇（6）,其结构经过IR,1H NMR,13C NMR,HRMS表征,并首次将其应用于催化二乙基锌对苯甲醛的不对称加成反应中,得到了较高的产率（82.8%）和较好的ee值（76.1%）. 相似文献
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阮鸣 《南京晓庄学院学报》2012,(3):80-83
以异黄芪皂苷含量为指标,高效液相色谱-蒸发光散射检测器（HPLC-ELSD）为检测方法,系统研究了D101大孔吸附树脂分离纯化异黄芪甲苷的吸附性能和洗脱参数.当上样液浓度为1 g.mL-1,上样体积（mL）∶树脂量（g）为3∶1时,D101大孔吸附树脂可100%吸附异黄芪甲苷;流速为5.0BV/h,4BV体积的蒸馏水、30%乙醇、50%乙醇和70%乙醇梯度洗脱时,70%乙醇洗脱液中异黄芪皂苷洗脱率达93.14%.实验结果表明D101大孔吸附树脂对异黄芪甲苷有较好的吸附和解吸性能,适合于异黄芪皂苷的分离纯化,可为大规模制备异黄芪甲苷提供参考. 相似文献
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微生物β-呋喃果糖苷酶在功能性低聚糖的工业中有广泛的应用前景,不同微生物来源的β-呋喃果糖苷酶特性和作用方式均不同,因而在不同的功能性低聚糖生产中有不同的应用特点。本文综述了微生物β-呋喃果糖苷酶的产生、特性及其在功能性低聚糖生产中的应用。 相似文献
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鄂永胜 《辽宁科技学院学报》2009,11(3):23-24
摘要：文章简要介绍了目前β-甲基萘分离精制的工艺研究现状,在此基础上经过多次实验,提出一种新的β-甲基蔡生产方法,即把工业甲基萘经两次酸洗后,一次精馏就得到含量大于96％的产品,收率可达60％,高于国内现有生产水平。 相似文献
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大量研究发现AD患者脑神经纤维tau蛋白的过度磷酸化形成NFTs,和Aβ在脑内的沉积形成老年斑,是导致AD发病的重要机制。在查阅了大量国内外有关tau蛋白和Aβ及运动对二者影响的文献的基础上,对导致tau蛋白异常过度磷酸化和Aβ产生的机制进行阐述,并就运动训练对tau蛋白过度磷酸化和Aβ的影响及防治AD的可能机制进行探讨。研究发现运动训练抑制了AD实验动物脑内tau蛋白的异常过度磷酸化和Aβ的沉积,在运动人体科学领域为体育运动防治AD的研究提供了可行性依据,也为AD的防治提供了除药物治疗外的新方法。 相似文献
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β3肾上腺素能受体在脂肪代谢中起着非常重要的作用。β3-AIR基因第64位密码子变异导致色氨酸被精氨酸取代,该变异与肥胖、胰岛素抵抗及2型糖尿病发病提早密切相关。相关研究表明,β3-AR基因Trp64Arg变异可能会干扰肥胖者的运动疗法。提示为肥胖患者制定运动处方时应考虑该变异的影响。 相似文献