低氧训练对大鼠脑组织Ngb、HIF-1α、Bax和Bcl-2的影响     

范锦勤  林文弢  翁锡全 《体育学刊》2012,(1):129-133

探讨常压下模拟低氧(氧体积分数13.6%)训练对大鼠脑组织Ngb、HIF-1α、Bax和Bcl-2的影响,为运动和低氧适应提供理论参考。将50只9周龄雄性SD大鼠随机分为低住安静组、低住低练组、高住安静组、高住低练组和高住高练低练组,每组10只。训练组大鼠进行跑台训练,强度为常氧下35 m/min、低氧下30 m/min,持续运动1 h/d,5 d/周,持续4周。实验结束后,采用ELISΑ试剂盒分别检测大鼠脑组织Ngb、HIF-1α、Bax和Bcl-2水平,并计算Bax与Bcl-2的比值。结果发现:(1)与低住安静组比较,低住低练组HIF-1α、Bax和Bax/Bcl-2均升高(P<0.05或P<0.01);高住安静、高住低练和高住高练低练组的Ngb、HIF-1α、Bax和Bcl-2均升高(P<0.05或P<0.01)。(2)与低住低练组相比,高住安静、高住低练和高住高练低练组的HIF-1α和Bcl-2均升高(P<0.01);高住低练和高住高练低练组的Bax/Bcl-2降低(P<0.01);高住高练低练组的Ngb升高(P<0.01)。(3)与高住安静组相比,高住低练组和高住高练低练组的HIF-1α、Bcl-2均升高(P<0.05或P<0.01);高住高练低练组的Ngb升高(P<0.01),Bax/Bcl-2降低(P<0.05)。(4)与高住低练组相比,高住高练低练组Bcl-2升高(P<0.01)。(5)Ngb表达和HIF-1α表达呈正相关(r=0.563,P<0.01);Ngb表达与Bax/Bcl-2变化呈正相关(r=0.486,P<0.01);HIF-1α表达与Bax/Bcl-2变化呈正相关(r=0.353,P<0.05)。结果表明:单纯训练刺激会引起大鼠脑组织HIF-1α升高,单纯低氧刺激会引起大鼠脑组织Ngb和HIF-1α升高,当训练和低氧这两种因素相互结合时,Ngb和HIF-1α的升高更明显。高住高练低练对大鼠脑组织Bcl-2的影响要大于高住低练。Ngb和HIF-1α的升高,使Bax/Bcl-2向着有利于神经元存活的方向发展,提示Ngb和HIF-1α参与了中枢神经系统的缺氧耐受和自我保护。  相似文献   

中药"强身液"的抗疲劳作用   总被引：3，自引：0，他引：3  

史亚丽  周世章  丁永玺 《体育学刊》2003,10(4):60-61

为观察以人参、黄芪为主要成分的“强身液”的抗疲劳作用,将强身液以0．1mL／d灌胃小鼠30d,测定小鼠耐缺氧能力、抗疲劳、疲劳恢复速度及安静时肌糖元、肝糖元含量。结果显示：实验组小鼠耐缺氧能力及运动能力明显提高,大强度运动后即刻血乳酸水平有下降趋势,运动后血乳酸恢复速度明显提高,安静时肝糖元、肌糖元水平明显升高。“强身液”可提高机体有氧代谢水平,提高机体运动能力,延缓疲劳及促进疲劳消除。  相似文献   

间歇性低氧训练对大鼠心肌、骨骼肌有氧代谢酶与运动能力的影响研究   总被引：10，自引：0，他引：10  

代毅  柯遵渝 《成都体育学院学报》2003,29(5):91-93

本实验借助于动物模型,探讨间歇性低氧训练后心肌和骨骼肌有氧代谢酶的变化与运动能力的关系。实验结果显示:经低氧训练后,大鼠心肌和骨骼肌的线粒体氧化酶SDH和CCO活性明显高于平原对照组和平原训练组,低氧训练后的大鼠运动时间延长。提示心肌和骨骼肌有氧代谢酶活性增高是提高有氧运动能力的重要物质基础。  相似文献   

玫瑰红景天的药理研究     

顾贤臣  常洁琴  崔广智 《河北北方学院学报(医学版)》1993,(2)

小鼠灌服玫瑰红景天水煎剂10g/kg,有加强硫贲妥纳的催眠作用,拮抗咖啡因的惊厥作用,抗疲劳、耐缺氧、耐饥饿,耐寒作用.小鼠皮下注射玫瑰红景天水煎剂5.0g/kg,可明显抑制皮下注射肾上腺素1.0mg/kg 后1h 引起的血糖升高。此外,玫瑰红景天水煎剂可使离体蛙心收缩幅度增加,使离体肠管收缩运动变得有节律.  相似文献   

浅析高原训练对人体生理机能的影响     

刘正荣  杨冰 《楚雄师范学院学报》2008,23(6)

随着竞技体育的不断发展和运动技术水平的提高,高原训练越来越受到国内外体育界的重视。高原训练的实践经验和基础理论的研亢得到了重视和完善。本文就高原训练对人体生理机能的影响等方面的研究进行综述。  相似文献   

实验性高海拔对犬SDH、LDH活性与骨骼肌超微结构的影响及其运动学意义     

贾磊  聂秀娟  方梅 《吉林体育学院学报》2010,26(1):60-62

通过对实验性不同海拔地区犬心肌、骨骼肌中SDH、LDH含量及其骨骼肌超微结构特点进行的比较研究,结果发现适度高原低氧环境诱导SDH活性显著增强,骨骼肌纤维糖原的积累和线粒体功能增强。并且对LDH酶的活性也有一定的促进作用。其运动学的意义在于适度的高原低氧环境训练对耐力运动项目及短距离的项目均有积极的意义。  相似文献   

Prioritizing natural-selection signals from the deep-sequencing genomic data suggests multi-variant adaptation in Tibetan highlanders     

Lian Deng  Chao Zhang  Kai Yuan  Yang Gao  Yuwen Pan  Xueling Ge  Yaoxi He  Yuan Yuan  Yan Lu  Xiaoxi Zhang  Hao Chen  Haiyi Lou  Xiaoji Wang  Dongsheng Lu  Jiaojiao Liu  Lei Tian  Qidi Feng  Asifullah Khan  Yajun Yang  Zi-Bing Jin  Jian Yang  Fan Lu  Jia Qu  Longli Kang  Bing Su  Shuhua Xu 《国家科学评论(英文版)》2019,6(6):1201

Human genetic adaptation to high altitudes (>2500 m) has been extensively studied over the last few years, but few functional adaptive genetic variants have been identified, largely owing to the lack of deep-genome sequencing data available to previous studies. Here, we build a list of putative adaptive variants, including 63 missense, 7 loss-of-function, 1,298 evolutionarily conserved variants and 509 expression quantitative traits loci. Notably, the top signal of selection is located in TMEM247, a transmembrane protein-coding gene. The Tibetan version of TMEM247 harbors one high-frequency (76.3%) missense variant, rs116983452 (c.248C > T; p.Ala83Val), with the T allele derived from archaic ancestry and carried by >94% of Tibetans but absent or in low frequencies (<3%) in non-Tibetan populations. The rs116983452-T is strongly and positively correlated with altitude and significantly associated with reduced hemoglobin concentration (p = 5.78 × 10⁻⁵), red blood cell count (p = 5.72 × 10⁻⁷) and hematocrit (p = 2.57 × 10⁻⁶). In particular, TMEM247-rs116983452 shows greater effect size and better predicts the phenotypic outcome than any EPAS1 variants in association with adaptive traits in Tibetans. Modeling the interaction between TMEM247-rs116983452 and EPAS1 variants indicates weak but statistically significant epistatic effects. Our results support that multiple variants may jointly deliver the fitness of the Tibetans on the plateau, where a complex model is needed to elucidate the adaptive evolution mechanism.  相似文献   

间歇性低氧训练对心理反应能力影响的实验研究   总被引：1，自引：0，他引：1  

陈耕春  黄东 《西安体育学院学报》2000,17(3):84-85,93

根据一些资料报道 ,间歇性低氧训练是一种利用低氧仪模拟高原训练的方法 ,它能够提高呼吸系统和循环系统的抗缺氧能力。为了研究它对心理反映能力的影响效果 ,本文作者对 15名女大学生进行了实验研究。结果显示 ,实验组被试除两人在两项心理测试中没有进步外 ,所有被试心理测试的成绩都取得较大的进步 ;实验组和控制组的心理测试结果存在较大的差异 ,实验组好于控制组。研究显示间歇性低氧训练对提高缺氧条件下的心理反应能力有明显的效果。  相似文献   

湖州地区鱼浮头强度指数的预报     

陈卫锋  林建华  杨咏钢  马传黎  向华 《科技通报》2005,21(2):153-156,170

对湖州市菱湖镇1991—2001年的鱼浮头观测资料进行统计,分析了鱼浮头与同期的气象因子的关系。将数值预报和模式预报相结合,通过指标归纳和逐步回归的方法建立鱼浮头强度指数预报方程。业务运用结果显示,该预报方法对鱼浮头有较强的预报能力。  相似文献   

Normobaric hypoxia increases the growth hormone response to maximal resistance exercise in trained men     

Dean Filopoulos  Stuart J. Cormack 《European Journal of Sport Science》2017,17(7):821-829

This study examined the effect of hypoxia on growth hormone (GH) release during an acute bout of high-intensity, low-volume resistance exercise. Using a single-blinded, randomised crossover design, 16 resistance-trained males completed two resistance exercise sessions in normobaric hypoxia (HYP; inspiratory oxygen fraction, (FiO₂) 0.12, arterial oxygen saturation (SpO₂) 82?±?2%) and normoxia (NOR; FiO₂ 0.21, SpO₂ 98?±?0%). Each session consisted of five sets of three repetitions of 45° leg press and bench press at 85% of one repetition maximum. Heart rate, SpO₂, and electromyographic activity (EMG) of the vastus lateralis muscle were measured throughout the protocol. Serum lactate and GH levels were determined pre-exposure, and at 5, 15, 30 and 60?min post-exercise. Differences in mean and integrated EMG between HYP and NOR treatments were unclear. However, there was an important increase in the peak levels and area under the curve of both lactate (HYP 5.8?±?1.8 v NOR 3.9?±?1.1?mmol.L^?1 and HYP 138.7?±?33.1 v NOR 105.8?±?20.8?min.mmol.L^?1) and GH (HYP 4.4?±?3.1 v NOR 2.1?±?2.5?ng.mL^?1 and HYP 117.7?±?86.9 v NOR 72.9?±?85.3?min.ng.mL^?1) in response to HYP. These results suggest that performing high-intensity resistance exercise in a hypoxic environment may provide a beneficial endocrine response without compromising the neuromuscular activation required for maximal strength development.  相似文献