Inhibitive effects of anti-oxidative vitamins on mannitol-induced apoptosis of vascular endothelial cells     

Pan KY  Shen MP  Ye ZH  Dai XN  Shang SQ 《Journal of Zhejiang University. Science. B》2006,7(10):825-829

Objective： Study blood vessel injury and gene expression indicating vascular endothelial cell apoptosis induced by mannitol with and without administration of anti-oxidative vitamins. Methods： Healthy rabbits were randomly divided into four groups. Mannitol was injected into the vein of the rabbit ear in each animal. Pre-treatment prior to mannitol injection was per- formed with normal saline （group B）, vitamin C （group C） and vitamin E （group D）. Blood vessel injury was assessed under electron and light microscopy. In a second experiment, cell culture specimen of human umbilical vein endothelial cells were treated with mannitol. Pre-treatment was done with normal saline （sample B）, vitamin C （sample C） and vitamin E （sample D）. Total RNA was extracted with the original single step procedure, followed by hybridisation and analysis of gene expression. Results： In the animal experiment, serious blood vessel injury was seen in group A and group B. Group D showed light injury only, and normal tissue without pathological changes was seen in group C. Of all 330 apoptosis-related genes analysed in human cell culture specimen, no significant difference was seen after pre-treatment with normal saline, compared with the gene chip without pre-treatment. On the gene chip pre-treated with vitamin C, 45 apoptosis genes were down-regulated and 34 anti-apoptosis genes were up-regulated. Pre-treatment with vitamin E resulted in the down-regulation of 3 apoptosis genes. Conclusion： Vitamin C can protect vascular endothelial cells from mannitol-induced injury.  相似文献   

Optimal design of pressure vessel using an improved genetic algorithm     

Peng-fei Liu  Ping Xu  Shu-xin Han  Jin-yang Zheng 《浙江大学学报(A卷英文版)》2008,9(9):1264-1269

As the idea of simulated annealing （SA） is introduced into the fitness function, an improved genetic algorithm （GA） is proposed to perform the optimal design of a pressure vessel which aims to attain the minimum weight under burst pressure constraint. The actual burst pressure is calculated using the arc-length and restart analysis in finite element analysis （FEA）. A penalty function in the fitness function is proposed to deal with the constrained problem. The effects of the population size and the number of generations in the GA on the weight and burst pressure of the vessel are explored. The optimization results using the proposed GA are also compared with those using the simple GA and the conventional Monte Carlo method.  相似文献   

Successful treatment of refractory pure red cell aplasia with eltrombopag after ABO-incompatible allogeneic hematopoietic stem cell transplantation     

Gao  Yang  Gao  Fei  Shi  Jimin  Fu  Huarui  Huang  He  Zhao  Yanmin 《Journal of Zhejiang University. Science. B》2021,22(8):695-700

Journal of Zhejiang University-SCIENCE B -...  相似文献   

Urinary donor-derived cell-free DNA as a non-invasive biomarker for BK polyomavirus-associated nephropathy     

Shen  Jia  Guo  Luying  Lei  Wenhua  Liu  Shuaihui  Yan  Pengpeng  Liu  Haitao  Zhou  Jingyi  Zhou  Qin  Liu  Feng  Jiang  Tingya  Wang  Huiping  Wu  Jianyong  Chen  Jianghua  Wang  Rending 《Journal of Zhejiang University. Science. B》2021,22(11):917-928

Journal of Zhejiang University-SCIENCE B - BK polyomavirus-associated nephropathy (BKPyVAN) is a common cause of allograft failure. However, differentiation between BKPyVAN and type I T...  相似文献   

TGF-β1 treated murine dendritic cells are maturation resistant and down-regulate Toll-like receptor 4 expression     

牟海波  林茂芳  岑洪  俞静  孟筱坚 《浙江大学学报(A卷英文版)》2004,5(10):1239-1244

  相似文献   

应用双剪理论计算钢管混凝土需注意的几点问题     

王福建  邱佩璜  徐彬轩 《科技通报》2006,22(4):531-534

针对钢管混凝土的复杂受力特性及目前计算钢管混凝土承载力时双剪统一理论的应用条件,通过对构件受压过程的理论分析得出应用该理论时常被工程忽视的几点关键问题。本文的分析有助于对钢管混凝土受力行为的深入理解,并为后续工作的展开提供指导。  相似文献   

大力发展科技咨询业 促进浙江经济发展     

陈明欣  许品琴 《科技通报》1996,12(5):312-316

蓬勃发展的科技咨询业是实现决策科学化，民主化的重要环节，是提高企事业单位及各类工程的经济效益与社会效益的重要途径，是科技商品化，市场化的纽带，在实施浙江省“科教兴省”的宏伟战略目标中起着越来越重要的作用，如何促进科技咨询业的迅速发展，对于推推动科学与经济结合，优化资源配置，促进市场经济有序发展，实现浙江经济腾飞，具有深远的意义。  相似文献   

Sensory exotropia subsequent to senile cataract     

楼定华  许叶圣  李毓敏 《Journal of Zhejiang University. Science. B》2005,6(12):1220-1222

INTRODUCTION Sensory exotropia is a condition of unilateral divergence as a sequela to loss of vision or long-standing poor vision in one eye,which was caused by refractive errors,unilateral aphakia or other organic reasons.Convergence insufficiency or disruption is possible.The most important aspect of the manage-ment is to find and/or eliminate and/or reverse a treatable cause of the exotropia.In clinic,the sensory exotropia secondary to senile cataract was often ob-served.Prospectively…  相似文献   

Comparison of epidemiological and clinical characteristics of COVID-19 patients with and without Wuhan exposure     

Lian  Jiang-shan  Cai  Huan  Hao  Shao-rui  Jin  Xi  Zhang  Xiao-li  Zheng  Lin  Jia  Hong-yu  Hu  Jian-hua  Zhang  Shan-yan  Yu  Guo-dong  Gu  Jue-qing  Ye  Chan-yuan  Jin  Ci-liang  Lu  Ying-feng  Sheng  Ji-fang  Yang  Yi-da 《Journal of Zhejiang University. Science. B》2020,21(5):369-377

Journal of Zhejiang University-SCIENCE B - A novel coronavirus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first identified in Wuhan, China, has been rapidly spreading...  相似文献   

Intrathymic inoculation of donor liver specific antigen alleviates rejection of liver allotransplantation     

贾长库  郑树森  章爱斌 《浙江大学学报(A卷英文版)》2003,4(4):485-490

Use and effects of liver specific antigen in orthotopic liver transplantations were researched in this study. Group I: syngeneic control (Wistar-to-Wistar); Group II: acute rejection (SD-to-Wistar); Group III: Thymic inoculation of SD rat LSA day 7 before transplantation. The observation of common situation and survival time, rejection grades, NF-κB activity of splenocytes and IL-2mRNA expression of grafted liver were used to analyze acute rejection severity and immune state of animals in different groups. The common situation of group I was very well after transplantation and no signs of rejection were found. Recipients of group II lost body weight progressively All dead within day 9 to day 13 posttransplantation; median survival time was 10.7 ±0.51 days. It was an optimal acute rejection control. As for group III, 5 out of 6 recipients survived for a long time and common situation was remarkably better than that of group II. Its rejection grades were significantly lower than that of group II(P<0.05). NF-κB activity was only detected in group I at day 5 and day 7 after transplantation, whereas high activity of NF-κB was detected at all time points in groupII and the low NF-κB activity detected in group III was significantly lower than that of group II (P<0.05). No IL-2mRNA expression was detected at any time point in group I, whereas high level expression was detected at all time points in group II and the low level expression only detected at day 3 in group III was significantly lower than that of group II (P<0.05). Conclusion: LSA is an important transplantation antigen which is involved directly in the immunorejection of liver transplantation. We report here for the first time that intrathymic inoculation of LSA can alleviate the rejection of liver allotransplantation; and that grafts can survive for a long time thereby, thus leading to a novel way to achieve liver transplantation immunotolerance. Project (NO. 011106206) supported by the Science and Technology Bureau of Zhejiang Province Foundation  相似文献