被子植物精细胞分离和特性研究进展     

朱维红  张妍  崔彬彬 《保定学院学报》2020,(3):114-121

精细胞分离是研究雄性生殖发育的前提之一,近半个世纪以来,人们运用不断更新的生物实验技术,对被子植物精细胞开展了分离及细胞分子特征的研究。分析被子植物精细胞分离方法,总结精细胞二型性与倾向性、细胞融合方面的研究成果,并对其研究意义和前景进行讨论。  相似文献   

集训对舞龙队女大学生血常规及内皮祖细胞的影响     

孔明涯  任路遥 《体育科技文献通报》2020,(4):156-158

目的:分析舞龙运动对女大学生血常规及内皮祖细胞含量的影响。方法:选取2017级舞龙队女大学生10人为训练组,2017级普通女大学生10人作为对照组。舞龙队女大学生备战比赛进行连续4周,每周3次,每次90min的赛前集训,普通女大学生除日常活动外,没有额外进行任何其他运动队的集训。集训结束后,在受试者都处于空腹状态下对两组受试者进行静脉采血并进行血常规、早期内皮祖细胞和晚期内皮祖细胞的检测。所得数据进行统计学分析。结果:参加舞龙集训的女大学生的血常规指标与普通女大学生的血常规指标无显著差异,早期EPCs和晚期EPCs无显著性差异,但在数值上显示出不同的趋势。结论:参加舞龙集训的女大学生身体处于健康状态。参加舞龙集训有可能增强运动员血管的自我修复能力。  相似文献   

低负荷加压训练改善自发性高血压大鼠的血压效果及机制研究     

谭朝文  赵彦  俞莹莹  马晓缓  郑玉婵 《体育科学》2020,(3):46-53,63

目的:探究低负荷加压训练对自发性高血压大鼠的降压效果及其作用机制。方法:选取4周龄雄性自发性高血压大鼠,随机分为对照组(高血压安静组)、低负荷训练组、低负荷加压训练组和高负荷训练组。低负荷训练组进行35%~55%1RM递进式低负荷爬梯训练,低负荷加压训练组进行30%~40%血流受限结合35%~55%1RM递进式低负荷爬梯训练,高负荷训练组进行55%~75%1RM递进式高负荷爬梯训练,训练后测定血压、血液中内皮素-1、血管内皮生长因子、一氧化氮合成酶的表达和心肌组织中内皮型一氧化氮合成酶的表达。结果:1)与对照组、高负荷训练组相比,低负荷加压训练组收缩压、舒张压显著下降(P<0.05);与低负荷训练组相比,低负荷加压训练组舒张压显著下降(P<0.05)。2)与对照组相比,低负荷加压训练组血液中内皮素-1表达显著下调(P<0.05),血管内皮生长因子和一氧化氮合成酶表达显著上调(P<0.05);3)与对照组相比,低负荷加压训练组心肌中内皮型一氧化氮合成酶表达显著上调(P<0.05);4)在低负荷加压训练组中,收缩压与内皮素-1呈正相关,相关性分析具有统计学意义(P<0.05);收缩压与一氧化氮合成酶、血管内皮生长因子、内皮型一氧化氮合成酶均呈负相关,相关性分析具有统计学意义(P<0.05)。结论:1)低负荷加压训练降压效果优于高负荷训练;2)低负荷加压训练能够通过下调血液中内皮素-1的表达,上调血液中血管内皮生长因子和一氧化氮合成酶的表达,同时上调心肌中内皮型一氧化氮合成酶表达,改善内皮细胞功能,达到降压的效果。  相似文献   

血管内皮生长因子和白细胞介素-12在急性白血病中的表达及临床意义     

高丽君  夏薇  杜冰洋 《中国科学院研究生院学报》2007,24(3):368-371

目的 通过检测急性白血病（AL）患者静脉血清中血管内皮生长因子（VEGF）和 白细胞介素12（IL-12）的含量，探讨VEGF和IL-12在急性白血病中的含量及临床意义。方法 应用定量酶联免疫吸附实验测定10例初诊未治、10例缓解期、5例复发患者和9例正常对照血清中VEGF和IL-12的含量。结果 初诊未治组的VEGF含量（521.06±163.85pg/ml）明显高于缓解组（307.62±55.40pg/ml）及对照组（262.01±141.66pg/ml）（p均<0.05）。对照组的IL-12水平（58.96±38.11pg/ml）与初诊复发组（初诊未治组与复发组的合称32.51±14.58pg/ml）、缓解组（71.67±119.09pg/ml）之间均有显著性差异（p均<0.05）。正常对照组VEGF的含量与IL-12之间存在负相关性。结论 VEGF和IL-12与AL的病情变化有一定的关系。  相似文献   

循环流化床热水锅炉燃烧控制策略     

王文才  李志 《黑龙江科技信息》2007,(10S):42-42

介绍了循环流化床热水锅炉的技术特点及其在供热领域的应用。对其自动控刺系统特别是燃料控制系统，鼓风变频系统。引风变频系统进行分析研究，以期获得最佳控制方案。  相似文献   

Induction of diabetes by Streptozotocin in rats   总被引：1，自引：0，他引：1  

A. Akbarzadeh  D. Norouzian  M. R. Mehrabi  Sh. Jamshidi  A. Farhangi  A. Allah Verdi  S. M. A. Mofidian  B. Lame Rad 《Indian journal of clinical biochemistry : IJCB》2007,22(2):60-64

The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats, makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases.  相似文献   

亚硝化-反硝化固定化细胞捷径生物脱氮的研究   总被引：1，自引：0，他引：1  

廖雪义  蓝荣 《襄樊学院学报》2008,29(8)

选用聚乙烯醇(PVA)和海藻酸钠(CA)作为载体,添加适量SiO2粉末,将筛选出来的高效亚硝化细菌和反硝化细菌分别制成固定化细胞,联合进行脱氮实验.结果表明:按1:1的比例,亚硝化细菌和反硝化细菌固定化细胞接种量为15%(W/V),在温度为30℃,摇床转数为110rpm的条件下处理合成废水(NH4+-N=150mg/L,碱度(NaHCO3=1700mg/L,pH=8.0),27h,脱氮率为97.53%;处理富营养化水体(NH4+-N=34.26mg/L,pH=6.7),20h,脱氮率达到79.51%.  相似文献   

在EXCEL中使用VBA编程处理数据   总被引：4，自引：0，他引：4  

王强 《福建教育学院学报》2003,4(10):84-87

本文通过利用EXCEL中的VBA编写宏来自动生成全国等级考试机试安排表的实例 ,说明EXCEL中宏的应用和单元格引用的方法。  相似文献   

新生儿脐血细胞检测分析     

尹卫东  刘克芹  刘晨  胡晓燕  商万军  李红丽 《河北北方学院学报(医学版)》2001,18(6):3-5

目的探讨新生儿脐静脉血细胞的特点,为新生儿脐血细胞参考值调查及新生儿健康情况提供资料.方法用Sysmex K-4500全自动血细胞分析仪检测51例脐静脉血及50例成人静脉血,并作统计分析,性别间、组间比较采用均数的t检验.结果发现新生儿脐血细胞测定值不同于新生儿末梢血测定值,在男女间MCV、PLT差异有极显著性意义(P＜0.01),RDW差异有显著性意义(P＜0.05);此外,脐血细胞PDW、MPVR、及P-LCR在剖宫产组、胎吸组与顺产组的差异有极显著性意义(P＜0.01).结论新生儿脐血细胞参数是一组特殊资料,反映了新生儿在宫内的发育状况,并与不同分娩方式有关.  相似文献   

Edaravone protects PC12 cells from ischemic-like injury via attenuating the damage to mitochondria   总被引：6，自引：0，他引：6  

Song Y  Li M  Li JC  Wei EQ 《Journal of Zhejiang University. Science. B》2006,7(9):749-756

Background： Edaravone had been validated to effectively protect against ischemic injuries. In this study, we investigated the protective effect of edaravone by observing the effects on anti-apoptosis, regulation of Bcl-2/Bax protein expression and recovering from damage to mitochondria after OGD （oxygen-glucose deprivation）-reperfusion. Methods： Viability of PC 12 cells which were injured at different time of OGD injury, was quantified by measuring MTT （2-（4,5-dimethylthia-zol-2-yl）-2,5-diphenyltetrazolium bromide） staining. In addition, PC 12 cells＇ viability was also quantified after their preincubation in different concentration of edaravone for 30 min followed by （OGD）. Furthermore, apoptotic population of PC 12 cells that reinsulted from OGD-reperfusion with or without preincubation with edaravone was determined by flow cytometer analysis, electron microscope and Hoechst/Pl staining. Finally, change of Bcl-2/Bax protein expression was detected by Western blot. Results： （1） The viability of PC12 cells decreased with time （1-12 h） after OGD. We regarded the model of OGD 2 h, then replacing DMEM （Dulbecco＇s Modified Eagle＇s Medium） for another 24 h as an OGD-reperfusion in this research. Furthermore, most PC 12 cells were in the state of apoptosis after OGD-reperfusion. （2） The viability of PC 12 cells preincubated with edaravone at high concentrations （1, 0.1, 0.01 μmol/L） increased significantly with edaravone protecting PC 12 cells from apoptosis after OGD-reperfusion injury. （3） Furthermore, edaravone attenuates the damage of OGD-reperfusion on mitochondria and regulated Bcl-2/Bax protein imbalance expression after OGD-reperfusion. Conclusion： Neuroprotective effects of edaravone on ischemic or other brain injuries may be partly mediated through inhibition of Bcl-2/Bax apoptotic pathways by recovering from the damage of mitochondria.  相似文献