长时间专项训练对男子摔跤运动员延迟性肌肉酸痛炎症反应的影响     

孙健 《沈阳体育学院学报》2004,23(3):372-374

观察15名男子古典摔跤运动员进行三周大强度专项训练对迟延性肌肉酸痛(DOMS)炎症反应的影响。第一周每日评定主观体力感觉等级(RPE)和肌肉酸痛程度,并分别在第一周的第一、三、五天和三周的第七天检测血清肌酸激酶(CK)及白细胞介素(IL 1β,6,8)水平。研究发现:第一周RPE从训练开始便出现显著升高,肌肉酸痛程度则第三、四、五天出现显著变化,第七天均恢复。CK、IL-6也均出现显著升高,IL-1β、IL 8则无显著变化。连续三周训练只有IL-6持续显著变化。提示:长时间大强度专项训练没有使DOMS症状加重,炎症反应也没有进一步恶化,机体已经对训练产生适应。  相似文献   

Walking and biologic ageing: Evidence based on NHANES telomere data     

Larry A. Tucker 《Journal of sports sciences》2020,38(9):1026-1035

ABSTRACT

The length of telomeres is an objective measure of biologic ageing. This study evaluated the extent minutes of walking per week are associated with leukocyte telomere length (LTL) in a random sample of 5,823 U.S. adults. The investigation was cross-sectional and data were obtained from the National Health and Nutrition Examination Survey (NHANES). LTL was measured by the quantitative polymerase chain reaction method. Walking minutes was calculated from walking frequency and duration measures. Results showed that for each year of chronological age, telomeres were 15.6 base pairs shorter (P < 0.0001). With walking minutes and LTL treated as continuous variables, the relationship was quadratic, not linear (F = 11.2, P = 0.0023). With walking time divided into three categories, adults who performed ≥ 150 minutes of walking per week had longer telomeres than those who did no regular walking, and those who did some, but less than the recommendation (F = 5.0, P = 0.0137). Regular walkers were estimated to have a biologic ageing advantage associated with 6.5–7.6 years less biologic ageing compared to non-walkers, after adjusting for covariates. Additional investigations designed to study causality and the mechanisms associated with the walking and LTL relationship are needed.  相似文献   

Long-term endurance training increases serum cathepsin S and decreases IL-6 and hsCRP levels     

Michael Sponder  Ioana-Alexandra Campean  Michael Emich  Monika Fritzer-Szekeres  Brigitte Litschauer  Jutta Bergler-Klein 《Journal of sports sciences》2017,35(21):2129-2134

Cathepsin S (CS) was shown to play a key role in cancer progression, atherosclerosis, heart valve disease, insulin resistance and diabetes mellitus. The present prospective study aimed to investigate the influence of sports on CS, interleukin-6 (Il-6) and high-sensitivity C-reactive protein (hsCRP) levels.

Ninety-eight of 109 participants completed the study. Ergometries were performed at baseline and after 8 months to evaluate/quantify the performance gain. Blood samples were taken at baseline and every 2 months. CS was measured by ELISA (enzyme-linked immunosorbent assay). Compared to the control group (mean performance gain ?3.41 ± 4.62%) we observed a significant physical-activity-induced increase of CS levels (3.45–3.73 ng · ml^?1; P = 0.027) and a significant decrease of Il-6 (2.43–1.91 pg · ml^?1; P = 0.031) and hsCRP-levels (0.11–0.09 mg · dl^?1; P = 0.001) in the intervention group (mean performance gain: 12.13 ± 6.32%). Furthermore, the tendency of the progression was significant for CS and Il-6 (P = 0.002/0.033).

We could show a significant sports-induced decrease of the classic inflammation parameters hsCRP/Il-6, probably expressing a downregulation of permanently prevalent inflammation processes. Simultaneously CS levels increased significantly. Our results show that increasing CS amounts are not simply to equal with an enhanced inflammation status and might even have beneficial effects on inflammation and angiogenesis.  相似文献   

Effects of 12 weeks of combined training without caloric restriction on inflammatory markers in overweight girls     

Wendell Arthur Lopes  Neiva Leite  Larissa Rosa da Silva  Diego Trevisan Brunelli  Arthur Fernandes Gáspari  Rosana Bento Radominski 《Journal of sports sciences》2016,34(20):1902-1912

The objective of the present study was to investigate the effects of combined training without caloric restriction on inflammatory markers in overweight girls. Thirty-three girls (13–17 years) were assigned into overweight training (n = 17) or overweight control (n = 16) groups. Additionally, a normal-weight group (n = 15) was used as control for the baseline values. The combined training programme consisted of six resistance exercises (three sets of 6–10 repetitions at 60–70% 1 RM) followed by 30 min of aerobic exercise (walking/running) at 50–80% VO_2peak, performed in the same 60 min session, 3 days/weeks, for 12 weeks. Body composition, dietary intake, aerobic fitness (VO_2peak), muscular strength (1 RM), glycaemia, insulinemia, lipid profile and inflammatory markers (C-reactive protein, interleukin-6, tumour necrosis factor-alpha, interleukin-10, leptin, resistin and adiponectin) were measured before and after intervention. There was a significant decrease in body fat (P < 0.01) and increase in fat-free mass (P < 0.01), VO_2peak (P < 0.01), 1 RM for leg press (P < 0.01) and bench press (P < 0.01) in the overweight training group. Concomitantly, this group presented significant decreases in serum concentrations of C-reactive protein (P < 0.05) and leptin (P < 0.05), as well as in insulin resistance (P < 0.05) after the experimental period. In conclusion, 12 weeks of combined training without caloric restriction reduced inflammatory markers associated with obesity in overweight girls.  相似文献   

Effect of repeat-sprint training in hypoxia on post-exercise interleukin-6 and F2-isoprostanes     

Paul S. R. Goods  Brian Dawson  Grant J. Landers  Christopher J. Gore  Kevin Croft  Peter Peeling 《European Journal of Sport Science》2016,16(8):1047-1054

This investigation examined the oxidative stress (F₂-Isoprostane; F₂-IsoP) and inflammatory (interleukin-6; IL-6) responses to repeat-sprint training in hypoxia (RSH). Ten trained male team sport athletes performed 3(sets)*9(repetitions)*5?s cycling sprints in simulated altitude (3000?m) and sea-level conditions. Mean and peak sprint power output (MPO and PPO) were recorded, and blood samples were collected pre-exercise, and again at 8 and 60?min post-exercise. Both MPO and PPO were significantly reduced in hypoxia (compared to sea-level) in the second (MPO: 855?±?89 vs. 739?±?95?W, p?=?.006; PPO: 1024?±?114 vs. 895?±?112?W, p?=?.010) and third (MPO: 819?±?105 vs. 686?±?83?W, p?=?.008; PPO: 985?±?125 vs. 834?±?99?W, p?=?.008) sets, respectively. IL-6 was significantly increased from pre- to 1?h post-exercise in both hypoxia (0.7?±?0.2 vs. 2.4?±?1.4?pg/mL, p?=?.004) and sea-level conditions (0.7?±?0.2 vs. 1.6?±?0.3?pg/mL, p?d?=?0.80) suggesting higher IL-6 levels of post-hypoxia. F₂-IsoP was significantly lower 1?h post-exercise in both the hypoxic (p?=?.005) and sea-level (p?=?.002) conditions, with no differences between trials. While hypoxia can impact on exercise intensity and may result in greater post-exercise inflammation, it appears to have little effect on oxidative stress. These results indicate that team sport organisations with ready access to hypoxic training facilities could confidently administer RSH without significantly increasing the post-exercise inflammatory or oxidative stress response.  相似文献   

不同剂量郑氏三七散动物肾细胞毒理学研究     

曹雪 《成都体育学院学报》2015,(4):118-121

目的：通过对大鼠服用不同剂量的郑氏三七散进行干预实验,以此了解郑氏三七散的毒理学性质。方法：实验选取SD雄性大鼠48只,随机分为四组：空白对照组、常规剂量组,中等剂量组和大剂量组,每组12只。干预实验持续3周。实验后抽取各组大鼠的血液检测,称取肾脏重量,留取肾组织标本观察。结果：（1）大鼠给药三周后,不同剂量组以及对照组的大鼠外观特征和行为活动表现正常,各组大鼠体重变化正常;（2）肾脏病理切片显示服用不同剂量郑氏三七散各组大鼠与对照组肾脏被膜完整,肾细胞结构完整,肾组织细胞未见坏死等异常现象出现;（3）各组大鼠肾脏重量及系数均无显著差异;（4）各组大鼠血液中尿素氮（BUN）和肌酐（Cr）无显著差异。结论：本实验条件下,三组不同剂量郑氏三七散使用时均比较安全,没有副作用,适合临床使用。  相似文献   

大剂量补充亮氨酸对抗阻运动所致骨骼肌损伤的影响     

夏志  赵艳  孟思进  史仍飞  尚画雨  王良峡  刘娟  苏全生 《北京体育大学学报》2017,40(10):56-62

摘要：目的：探讨补充亮氨酸对无训练史被试进行抗阻运动所致骨骼肌损伤的影响。方法：16名青年男性大学生据体重随机分为补充亮氨酸组与安慰剂对照组，亮氨酸和安慰剂组被试从测试日深蹲抗阻运动前3周直至运动后4 d分别以每千克体重150 mg剂量摄入150 mL亮氨酸或糊精溶液。测试日各组被试以50% 1RM负荷完成5组深蹲运动，每组20次，组间间歇2 min。深蹲运动前于肘正中静脉采集血样，测量大腿围、纵跳高度、膝关节活动幅度与肌肉酸痛程度，并于运动后即刻、24 h、48 h、72 h和96 h重复测试。ELISA法检测血清CK-MM、Mb与血浆IL-6，胶乳增强免疫比浊法检测血清CRP。结果：与安慰剂组被试相比，补充亮氨酸显著降低了亮氨酸组被试运动后延迟性肌肉酸痛水平、大腿中段周长及血清CK-MM、Mb、CRP与血浆IL-6含量，而深蹲1-RM值、纵跳高度及膝关节ROM组间差异则并无统计学意义。结论：1）每千克体重150 mg剂量亮氨酸能够削弱抗阻运动所导致的骨骼肌损伤与疼痛，该效应可能与对炎症反应的抑制有关，且推测亮氨酸对骨骼肌蛋白质代谢平衡的调节作用在其中亦有贡献；2）该剂量亮氨酸不能明显促进工作肌机能恢复。  相似文献   

Effect of supplementation of vitamin E, vitamin C and reduced glutathione on copper ion induced lipoprotein oxidation in renal diseased patients—Anin vitro study     

C. S. Parameswari  B. Vijayageetha  R. Vijayakumar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):131-136

The oxidative modification of lipoprotein especially low density lipoprotein (LDL) plays a key role in the initiation and progression of atherosclerosis. Serum apolipoproteinB (apoB) level is found to be an important marker for atherosclerosis. The present paper focuses on the measurement of serum apoB levels and the effect of Vitamin E, Vitamin C and reduced glutathione on the copper ion induced oxidation of LDL + VLDL (VLDL-Very Low Density Lipoprotein) lipoprotein fraction isolated from the serum of chronic renal failure (CRF) and renal transplanted patients. The level of apoB is significantly higher in the serum of transplanted patients when compared to CRF patients and normal subjects. The level of TBARS formed in oxidized LDL + VLDL lipoprotein fraction of CRF and renal transplanted patients are significantly increased than normal even in the presence of antioxidants but the level of TBARS produced, with antioxidants is comparatively lesser than that produced by oxidized lipoprotein fraction without antioxidants. It may be concluded than that produced by oxidized lipoprotein fraction without antioxidants. It may be concluded that oxidation of LDL can be prevented at an earlier stage by Vitamin E supplementation. The supplementation with Glutathione serves as the best method of preventing the lipoprotein oxidation among the renal diseased patients.  相似文献   

Enzymuria pattern in early post renal transplant period: Diagnostic usefulness in graft dysfunction     

Banibrata Mukhopadhyay  Shashikant Chinchole  Valentine Lobo  Sishir Gang  Mohan Rajapurkar 《Indian journal of clinical biochemistry : IJCB》2004,19(2):14-19

Serum creatinine does not distinguish between various causes of graft dysfunction. Serial assay of proximal tubular enzymes N-Acetyl-D-glucosaminidase (NAG), Alanine aminopeptidase (AAP) and Gamma glutamyl transferase (GGT) in urine was done to assess their usefulness in distinguishing various causes of graft dysfunction. Daily serum creatinine and enzymuria were measured in 32 consecutive renal allograft recipients for first 15 postoperative days. Graft dysfunction was defined as >20% increase in serum creatinine and >100% increase in enzymuria over the baseline. The diagnosis of graft dysfunction was based upon clinical criteria, ultrasonography, cyclosporin trough level, allograft biopsy, response to anti-rejection therapy and alteration of cyclosporin dosage. Fifteen episodes of graft dysfunction were identified in 15 patients. The sensitivity and specificity of the enzymes (NAG, AAP and GGT) for predicting graft dysfunction were 87.5%, 86.9%, 88.5% and 98.2%, 98.2%, 97.9% respectively. There was a significant increase in enzymuria during acute tubular necrosis (ATN) and acute rejection episode compared to cyclosporin nephrotoxicity (p<0.01). Enzymuria assay provides a simple, reliable and noninvasive method to distinguish cyclosporin nephrotoxicity from acute tubular necrosis and acute rejection in renal allograft recipients.  相似文献   

TLR4信号通路参与有氧运动对ApoE基因敲除小鼠血管炎性损伤的修复     

陈嘉勤  柳维林  张川湘  王茂材  李林 《体育科学》2011,31(8)

目的:通过中小强度有氧运动和辛伐他汀药物干预,探讨AS血管炎性损伤的修复及机制。方法:应用ApoE基因敲除和高脂高胆固醇饲料诱发小鼠AS,分为动脉硬化组、运动组、用药组;ELISA测定血清炎性因子及激素水平;免疫组化法阳性标记TLR4、NF-κb、EGF、TGF-β;RT-PCR测定CRP、MCP-1 mRNA表达。结果:12周动脉硬化组ApoE基因敲除小鼠血管可见明显斑块组织;用药组内皮表层细胞肿胀呈泡沫样,运动组则未见以上组织改变;运动和用药组小鼠血清IL-6、TNF-a含量和血管组织TLR4、NF-κb、CRP、MCP-1表达均降低(P<0.05),而血清IL-10、ET1、AngⅡ和内皮细胞EGF、TGF-βmRNA表达均升高(P<0.05)。结论:有氧运动有效防治AS小鼠血管炎性损伤,其可能机制是介导了Toll样受体通路并恢复血管激素分泌与促进内皮细胞再生。  相似文献