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验证收缩骨骼肌细胞葡萄糖转运蛋白4（GLUT4）基因的表达在转录水平上是否受转录抑制子Ⅱ型HDAC5蛋白（简写为HDAC5）的调节。研究运用运动模拟信号5-氨基-4-甲酰胺咪唑核糖核苷酸（AICAR）和HDACs抑制剂SCRIPTAID孵育骨骼肌细胞。用蛋白印迹技术测定HDAC5蛋白表达,定量RT-PCR法检测GLUT4 mRNA表达。与无AICAR的对照组相比,AICAR组骨骼肌细胞内HDAC5减少了29%,并伴随有124% GLUT4 mRNA的增加,但肌细胞内HDAC5的蛋白总量无变化;使用HDACs抑制剂SCRIPTAID刺激骨骼肌细胞能够明显增加核心组蛋白乙酰化水平和上调GLUT4基因的表达。这些结果提示,通过AICAR和SCRIPTAID分别减少或抑制核HDAC5均能上调骨骼肌细胞GLUT4基因的转录,HDAC5可能在转录水平上对肌肉收缩引起的GLUT4基因的表达起着重要的调节作用。 相似文献
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Theory of Mind: Deaf and Hearing Children's Comprehension of Picture Stories and Judgments of Social Situations 总被引：2，自引：0，他引：2
We compared 20 prelingually profoundly deaf adolescents (age: 11-16 years) and 20 matched, hearing adolescents on a picture-sequencing task and on a social judgment test. In addition, we also tested 14 younger deaf children (age: 6-10 years) and compared their data with those from 20 hearing peers as well as those from the older deaf participants on the picture-sequencing task. The results from this study did not provide evidence for the hypothesis that deaf adolescents possess significantly poorer knowledge about social reasoning than age-matched hearing peers, but it did present further additional support for Peterson and Siegal's (1995) conversational hypothesis: a proposal that a deprivation in conversations about mental states leads to an impairment in the development of an awareness of mental states in the younger deaf children. 相似文献
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