减少和抑制核HDAC5对骨骼肌细胞GLUT4基因表达的影响     

李良刚  陈槐卿  MCGEE SL 《体育科学》2007,27(12):20-26

验证收缩骨骼肌细胞葡萄糖转运蛋白4（GLUT4）基因的表达在转录水平上是否受转录抑制子Ⅱ型HDAC5蛋白（简写为HDAC5）的调节。研究运用运动模拟信号5-氨基-4-甲酰胺咪唑核糖核苷酸（AICAR）和HDACs抑制剂SCRIPTAID孵育骨骼肌细胞。用蛋白印迹技术测定HDAC5蛋白表达,定量RT-PCR法检测GLUT4 mRNA表达。与无AICAR的对照组相比,AICAR组骨骼肌细胞内HDAC5减少了29%,并伴随有124% GLUT4 mRNA的增加,但肌细胞内HDAC5的蛋白总量无变化;使用HDACs抑制剂SCRIPTAID刺激骨骼肌细胞能够明显增加核心组蛋白乙酰化水平和上调GLUT4基因的表达。这些结果提示,通过AICAR和SCRIPTAID分别减少或抑制核HDAC5均能上调骨骼肌细胞GLUT4基因的转录,HDAC5可能在转录水平上对肌肉收缩引起的GLUT4基因的表达起着重要的调节作用。  相似文献   

On a mode of preparing etching-ground for engravers     

Edmund Turrell 《Journal of The Franklin Institute》1826,2(2):83-86

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A longitudinal investigation of the dynamics of mental state talk in families   总被引：7，自引：0，他引：7  

Jenkins JM  Turrell SL  Kogushi Y  Lollis S  Ross HS 《Child development》2003,74(3):905-920

Thirty-seven mothers and fathers were observed in their homes interacting with their 2- and 4-year-old-children at Time 1 and 2 years later. Parental mental state talk to children varied as a function of children's age, the context in which talk occurred, and the gender of the parent. Four-year-old children, with an older sibling, produced and heard more cognitive talk and less desire talk than children without an older sibling. Cognitive and feeling talk by family members at Time 1 predicted change in younger children's cognitive and feeling talk (respectively) 2 years later, after controlling for initial levels of younger children's talk and general language ability. Findings are discussed in the context of theory of mind understanding and family talk about the mind.  相似文献