Call for papers for a special edition (Journalism and Sexual Violence) for Journalism Practice (2020, Vol 14, No 2).     

Andrea Baker  Usha M. Rodrigues 《Journalism Practice》2020,14(1):125-126

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Serum adenosine deaminase, 5′ nucleotidase and malondialdehyde in acute infective hepatitis     

K. Pratibha  Usha Anand  Rajni Agarwal 《Indian journal of clinical biochemistry : IJCB》2004,19(2):128-131

Serum adenosine deaminase (ADA), 5′ nucleotidase (5′NT) and malondialdehyde (MDA) were estimated in patients with acute infective hepatitis (AIH) along with the routine parameters of liver disease. Present study is done to evaluate these special parameters in patients with clinical history of AIH and to assess the utility of these parameters as diagnostic/ prognostic indices of liver function and to correlate special parameters with routine live function tests (LFT). ADA, 5′NT and MDA along with routine LFT was estimated in 25 patients with AIH and 25 samples from healthy voluntary blood donars served as the control group. Routine LFT was estimated by standard clinical chemistry procedures on dade behring analyser and ADA, 5′NT and MDA were estimated by berthlot reaction, fiske and subbarao method and thiobarbituric acid method respectively. Statistical analysis showed that serum ADA, 5′NT and MDA were significantly higher in patients as compared with the controls. There was a significant positive correlation between ADA and total bilirubin and MDA and total bilirubin. Hence we can conclude that these tests would be more sensitive to diagnose the patients with AIH and that the raised bilirubin levels could be looked upon, as a protective mechanism which the liver has evolved in order to combat oxidative stress.  相似文献   

Immunoproteomic analysis of secretory proteins ofAspergillus fumigatus with specific IGE immunoreactivity     

Poonam Gautam  Taruna Madan  W. N. Gade  P. Usha Sarma 《Indian journal of clinical biochemistry : IJCB》2006,21(2):12-19

Allergenic/antigenic proteins are known to induce Type I and Type III hypersensitivity reactions leading to allergic bronchopulmonary aspergillosis (ABPA) in immunocompetent host. The common structural features or intrinsic properties of the allergens/antigens leading to allergenicity in a host are not well understood. In the current report, comparative analysis of proteins on two dimensional gel electrophoresis (2D-3) and specific IgE immunoblots ofA. fumigatus secretory proteins (1^st, 2^nd and 3^rd week culture filtrate proteins) was carried out. We observed a total of 159 proteins in 1^st, 2^nd and 3^rd week culture filtrates ofA. fumigatus. Specific IgE immunoreactivity was observed in 75 proteins with different intensity. Third week culture filtrate showed maximum number of proteins, 142, and specific IgE immunoreactive proteins, 65. MALDI-TOF analysis resulted in putative identification of two allergens as hypothetical protein YBL057c fromSaccharomyces cereviseae and unnamed protein product fromDebaryomyces hansenii (similar to IPF14568 ofCandida albicans). Identification of a repertoire of specific IgE immunoreactive proteins will facilitate the studies on structure-function relationship of these proteins relevant for diagnosis and pathogenesis.  相似文献   

Astudy on mycobacterial proteins in the serodiagnosis of tuberculosis using ELISA and western blot     

Ajay Kumar  T. A. Venkita Subramanian  Usha P. Sharma 《Indian journal of clinical biochemistry : IJCB》1992,7(2):165-168

Protein fraction termed P₂ was obtained from sonicates of Mycobacterium smegmatis, subjected to ammonium sulphate precipitation. P₂ fraction was further fractionated into 4 fractions (PeakI-IV) by DEAE cellulose ion-exchange chromatography. ELISA was performed on the sera of 104 tuberculous cases and 62 controls, using P₂ fractions Peak I as test antigens. Though the mean ELISA values in tuberculous cases were higher than the controls, no statistically significant difference was found between the two. Antigens P₂, Peak I and IV were tested on Western blots with pooled sera from tuberculous cases as well as controls. P₂ fraction, Peak-I and IV were separated on PAGE-SDS, electroblotted onto nitrocellulose Sheets and the blots were subjected to ELISA. Peak IV appeared as a single band (M.W. 55,000). P₂ fraction exhibited some discriminatory bands on development of blots.  相似文献   

Myeloperoxidase in Chronic Kidney Disease     

Madhusudhana Rao A  Anand U  Anand CV 《Indian journal of clinical biochemistry : IJCB》2011,26(1):28-31

Numerous lines of evidence implicate a role of myeloperoxidase (MPO) in the pathogenesis of cardiovascular disease (CVD). It is a well accepted fact that patients with chronic kidney disease (CKD) are at an increased risk for CVD. MPO is a pro-oxidant enzyme which could be involved in the increased susceptibility of these patients to CVD. Hence, the levels of plasma MPO was determined in healthy controls as well as in patients with CKD [stratified with the level of their kidney failure as CKD stages II–V (end stage renal disease)]. Plasma MPO was assayed by a spectrophotometric method. Serum urea and creatinine were estimated on a clinical chemistry analyzer using standard laboratory procedures. The mean plasma MPO levels were significantly lower with advancing stages of renal failure (P < 0.001). There was a positive correlation between MPO and GFR (r = +0.89, P < 0.001) and a negative correlation with urea (r = −0.85, P < 0.001) and creatinine (r = −0.82, P < 0.001). While an inverse association was observed between plasma MPO and urea in CKD patients, such an association was not observed in control subjects (P = 0.43). In conclusion, the decline in plasma MPO levels may be due to the inhibitory effect of uraemic toxins on the enzyme.  相似文献   

Application of Sigma Metrics for the Assessment of Quality Assurance in Clinical Biochemistry Laboratory in India: A Pilot Study     

Singh B  Goswami B  Gupta VK  Chawla R  Mallika V 《Indian journal of clinical biochemistry : IJCB》2011,26(2):131-135

Ensuring quality of laboratory services is the need of the hour in the field of health care. Keeping in mind the revolution ushered by six sigma concept in corporate world, health care sector may reap the benefits of the same. Six sigma provides a general methodology to describe performance on sigma scale. We aimed to gauge our laboratory performance by sigma metrics. Internal quality control (QC) data was analyzed retrospectively over a period of 6 months from July 2009 to December 2009. Laboratory mean, standard deviation and coefficient of variation were calculated for all the parameters. Sigma was calculated for both the levels of internal QC. Satisfactory sigma values (>6) were elicited for creatinine, triglycerides, SGOT, CPK-Total and Amylase. Blood urea performed poorly on the sigma scale with sigma <3. The findings of our exercise emphasize the need for detailed evaluation and adoption of ameliorative measures in order to effectuate six sigma standards for all the analytical processes.  相似文献   

The Status of Proving Among US Secondary Mathematics Teachers     

Usha Kotelawala 《International Journal of Science and Mathematics Education》2016,14(6):1113-1131

This report examines teachers’ self-espoused attitudes and beliefs on proving in the secondary mathematics classroom. Conclusions were based on a questionnaire of 78 US mathematics teachers who had completed at least 2 years of teaching mathematics at the secondary level. While these teachers placed importance on proving as a general mathematical skill, when they discuss their own classrooms, procedural skill consistently surpasses proof-related activities in importance for a majority of high school teachers. Furthermore, teachers labeling their own past experiences in proving as causing anxiety are predictably more likely to put less value on proving. Interestingly, the quantity of past college mathematics courses is a reverse predictor indicating that further study should consider how students perceptions of proving change as they pass through a mathematics major.  相似文献   

Chemical research of Sir Prafulla Chandra Rây     

Sreebrata Goswami  Samaresh Bhattacharya 《Resonance》2001,6(1):42-49

The above account reveals the versatility of Prafulla Chandra’s research activities. Most importantly, he initiated chemical research in modern India and was successful in developing the first research school of chemistry that in time spread far and wide. In his obituary notice,Nature (July 15, 1944) wrote “it was by the enthusiasm for research with which he inspired his students that he will best be remembered”.  相似文献   

Learning about Spelling Sequences: The Role of Onsets and Rimes in Analogies in Reading   总被引：2，自引：0，他引：2  

Usha Goswami 《Child development》1991,62(5):1110-1123

Children's phonological awareness, especially their awareness of rhyme, is known to be importnat for later reading development. However, the exact nature of the connection between phonological skills and reading is not yet known. One possibility is that when children analyze written words in reading, they find it relatively easy to learn about spelling sequences within these words that reflect phonological categorizations such as rhyme. This hypothesis implies that learning to read words is not a purely visual process. For example, spelling sequences that reflect the intrasyllabic linguistic units of onset (initial consonants) and rime (vowel and final consonants), and thus are connected to rhyme, may be easier to learn about than other spelling sequences. In Experiment 1, children learned more about shared consonant blends at the beginnings of words ( tr im- tr ap), which constituted the onset, than at the ends of words (wi nk -ta nk ), which broke up the rime. In contrast, Experiment 2 showed that when words shared a vowel as well as a consonant blend, more was learned about spelling sequences at the ends of words (w ink -p ink ), which now reflected the rime, than at the beginnings of words ( tr im- tr ip), where the vowel extended the onset. So intrasyllabic phonological knowledge does seem to play a role in learning about spelling sequences in reading.  相似文献   

Iron store and free radicals in thalassemia     

K. Goswami  S. Ghosh  M. Bandyopadhyay  K. L. Mukherjee 《Indian journal of clinical biochemistry : IJCB》2005,20(2):192-194

Hyper-ferrous state is the result of multiple transfusions, whereby the iron excess in such conditions may be toxic to the organism. Patients suffering from thalassemia receive multiple transfusions. In the eastern part of India, both β⁰-thalassemia and heterozygous Eβ-thalassemia occur. This paper expresses the hyper-ferrous state, leading to iron overload in these two conditions and also reports the increased generation of free radicals, consequent to the iron overload.  相似文献