Effective extraction of elastase from Bacillus sp. fermentation broth using aqueous two-phase system   总被引：1，自引：0，他引：1  

Xu Y  He GQ  Li JJ 《Journal of Zhejiang University. Science. B》2005,6(11):1087-1094

INTRODUCTION Aqueous two-phase system (ATPS) is widely used in biochemistry and biotechnology for purifica- tion of proteins (Diamond and Hsu, 1992; Balasubramaniam et al., 2003), enzymes (Pan et al., 2001; Bim and Franco, 2000), amino acids (Li et al., 2002), antibiotics (Yang et al., 1994; Guan et al., 1994), nucleic acids (Ohlsson et al., 1978; Kimura, 2000), aroma compounds (Marco et al., 2000) and lactic acid (Planas et al., 1998). ATPS is mainly composed of two-incompatible p…  相似文献   

癌症的替代疗法     

Aulas  JJ 王世德 《科学中国》1997,(1):80-81

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欧洲科技发展战略探讨（二）     

张承友  曹增友 《科学学与科学技术管理》1989,10(4):30-31

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Relationship between hyperuricemia and metabolic syndrome   总被引：6，自引：0，他引：6  

Chen LY  Zhu WH  Chen ZW  Dai HL  Ren JJ  Chen JH  Chen LQ  Fang LZ 《Journal of Zhejiang University. Science. B》2007,8(8):593-598

Objective: To investigate the relationship between metabolic syndrome and hyperuricemia. Methods: A total of 2 374 subjects who received health examination in our hospital from Jan. 2004 to Dec. 2006 were enrolled in our study. Hyperuricemia is defined as ≥7 mg/dl (in men) or ≥6.0 mg/dl (in women). Metabolic syndrome was defined using AHA/NHLBI (American Heart Association/National Heart, Lung, and Blood Institute) criteria. Results: (1) The overall prevalence of hyperuricemia was 13.10%. The condition was more common in men than in women (19.07% vs 3.42%). (2) Among men, uric acid concentration is statistically significantly positively correlated with waist circumference, blood pressure, and triglyceride. Uric acid is negatively correlated with serum high-density lipoprotein-cholesterol (HDL-C). Uric acid concentration is most strongly correlated with serum triglyceride (r=0.379) and waist circumference (r=0.297). Among women, statistically significant positive correlations were noted for the serum uric acid concentrations with waist circumference, triglyceride and fasting plasma glucose. Serum triglyceride (r=0.329) and waist circumference (r=0.234) are most strongly correlated with uric acid concentrations. (3) Men with hyperuricemia had a 1.634-fold increased risk of metabolic syndrome as compared with those without hyperuricemia odds ratio (OR)=1.634, P=0.000. Women with hyperuricemia had a 1.626-fold increased risk of metabolic syndrome (OR=1.626, P=0.000) as compared with those without hyperuricemia. Conclusion: Hyperuricemia is prevalent among Chinese population. Additionally, serum uric acid is positively associated with metabolic syndrome.  相似文献   

Infant joint attention, temperament, and social competence in preschool children   总被引：2，自引：0，他引：2  

Vaughan Van Hecke A  Mundy PC  Acra CF  Block JJ  Delgado CE  Parlade MV  Meyer JA  Neal AR  Pomares YB 《Child development》2007,78(1):53-69

Infant joint attention has been observed to be related to social-emotional outcomes in at-risk children. To address whether this relation is also evident in typically developing children, 52 children were tested at 12, 15, 24, and 30 months to examine associations between infant joint attention and social outcomes. Twelve-month initiating and responding to joint attention were related to 30-month social competence and externalizing behavior, even when accounting for 15-month temperament ratings, 24-month cognition and language, and demographic variables. These results suggest that, in addition to associations with language and cognition, infant joint attention reflects robust aspects of development that are related to individual differences in the emergence of social and behavioral competence in childhood.  相似文献   

I like to do it, I'm able, and I know I am: longitudinal couplings between domain-specific achievement, self-concept, and interest     

Denissen JJ  Zarrett NR  Eccles JS 《Child development》2007,78(2):430-447

The longitudinal development of the intraindividual coupling between academic achievement, interest, and self-concept of ability (SCA) was analyzed in a sample of approximately 1,000 children between grades 1 and 12 (ages 6-17). Across all calculated indexes, the average level of coupling was positive. Individuals generally felt competent and interested in domains where they achieve well, and were interested in domains where they perceive their personal strengths. The degree of coupling was the highest between interest and SCA and the lowest between interest and achievement. For all indexes, evidence for an increase in coupling across time was found. Female gender was related to a lower level of coupling. There was evidence for a positive effect of conscientiousness on the amount of coupling.  相似文献   

Association of parent and peer support with adolescent physical activity     

Prochaska JJ  Rodgers MW  Sallis JF 《Research quarterly for exercise and sport》2002,73(2):206-210

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2009新春祝福     

JJ 《当代职校生》2009,(1)

牛年喜奏丰年乐；人世笑迎盛世春。走过了难忘的2008，经历了雪灾和令世人震撼的地震。经历了百年奥运，感受了国人的凝聚力和运动健儿的奥林匹克精神。2009年，新的高度、新的起点，崭新的一页将由你我共同书写。  相似文献   

Carbohydrate and carbohydrate + protein for cycling time-trial performance     

Osterberg KL  Zachwieja JJ  Smith JW 《Journal of sports sciences》2008,26(3):227-233

Carbohydrate intake during endurance exercise delays the onset of fatigue and improves performance. Two recent cycling studies have reported increased time to exhaustion when protein is ingested together with carbohydrate. The purpose of the present study was to test the hypothesis that ingestion of a carbohydrate + protein beverage will lead to significant improvements in cycling time-trial performance relative to placebo and carbohydrate alone. Thirteen cyclists completed 120 min of constant-load ergometer cycling. Thereafter, participants performed a time-trial in which they completed a set amount of work (7 kJ kg(-1)) as quickly as possible. Participants completed four experimental trials, the first for familiarization and then three randomized, double-blind treatments consisting of a placebo, carbohydrate, and carbohydrate + protein. Participants received 250 ml of beverage every 15 min during the constant-load ride. Time-trial performance for carbohydrate (37.1 min, s = 3.8) was significantly (P < 0.05) faster than placebo (39.7 min, s = 4.6). Time-trial performance for carbohydrate + protein (38.8 min, s = 5.5) was not significantly different from either placebo or carbohydrate. Ingestion of a carbohydrate beverage during two hours of constant-load cycling significantly enhanced subsequent time-trial performance compared with placebo. The carbohydrate + protein beverage provided no additional performance benefit.  相似文献   

Effect of three cooking methods on nutrient components and antioxidant capacities of bamboo shoot (Phyllostachys praecox C.D. Chu et C.S. Chao)     

Zhang JJ  Ji R  Hu YQ  Chen JC  Ye XQ 《Journal of Zhejiang University. Science. B》2011,12(9):752-759

Three cooking methods, namely boiling, steaming, and stir-frying for 5 to 10 min, were used to evaluate the effect on nutrient components, free amino acids, l-ascorbic acid, total phenolic contents, and antioxidant capacities of bamboo shoots (Phyllostachys praecox). Results showed that boiling and stir-frying had a great effect on the nutrient components and they decreased the contents of protein, soluble sugar, and ash, and caused a great loss in the total free amino acids (decreased by 38.35% and 34.86%, respectively). Significant differences (P<0.05) in free amino acids were observed in the samples cooked by different methods. Stir-fried bamboo shoots had a high fat content which increased by 528.57% because of the addition of edible oil. After boiling, the L-ascorbic acid and total phenolic contents were significantly reduced, while steaming increased total phenolic content by 3.98% and stir-frying well-preserved L-ascorbic acid (78.87% of its previous content). Results of the antioxidative property study showed that stir-frying could increase antioxidant capacities of bamboo shoots. It is concluded that stir-frying is more suitable for bamboo shoots because it could obtain the maximum retention of antioxidant capacities.  相似文献