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Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR drift, and primer-primer interactions. We present a new thermocycling method, termed thermal multiplexing, in which a single heat source is uniformly distributed and selectively modulated for independent temperature control of an array of PCR reactions. Thermal multiplexing allows amplification of multiple targets simultaneously—each reaction segregated and performed at optimal conditions. We demonstrate the method using a microfluidic system consisting of an infrared laser thermocycler, a polymer microchip featuring 1 μl, oil-encapsulated reactions, and closed-loop pulse-width modulation control. Heat transfer modeling is used to characterize thermal performance limitations of the system. We validate the model and perform two reactions simultaneously with widely varying annealing temperatures (48 °C and 68 °C), demonstrating excellent amplification. In addition, to demonstrate microfluidic infrared PCR using clinical specimens, we successfully amplified and detected both influenza A and B from human nasopharyngeal swabs. Thermal multiplexing is scalable and applicable to challenges such as pathogen detection where patients presenting non-specific symptoms need to be efficiently screened across a viral or bacterial panel.  相似文献   
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Reading and Writing - Argumentative writing has long been considered an essential skill for disciplinary learning. For researchers and curriculum developers to develop ecologically valid...  相似文献   
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The article outlines the life and work of Maryam Mirzakhani — the first and the only female winner of the Fields Medal since its inception in 1936. Mirzakhani is an inspiration to young women around the world, to believe in their own abilities and pursue their academic dreams.  相似文献   
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In this paper we suggest the need for research which addresses school choice as a global phenomenon. That is, a form of choice which extends beyond local politics and policymaking. We consider here the educational choices and choice making contexts of a burgeoning, mobile, post-national middle class who operate on a global scale. We also sketch the educational market within which these choices are considered and realised but the main focus is on demand side issues. The paper seeks to open a space for further research, in which to ask some old and some new questions about social class and social reproduction through schooling, and to make the case for choice researchers to attend more carefully to choice in a framework of mobility, globalisation and related new kinds of social class identities and interests.  相似文献   
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The current business landscape has created the impetus to develop management graduates with capabilities that foster responsible leadership and sustainability. Through the lens of Gitsham's 3C Model (Complexity, Context and Connection) of graduate capabilities, this paper discusses the experience of implementing the United Nations Principles for Responsible Management Education (UNPRME) at the Graduate School of Management, La Trobe University Australia. The case highlights that universities should use both a top-down and bottom-up approach, engaging students in the implementation process. The analysis also shows that educational institutions face similar challenges to business organizations in terms of resource limitations, inertia and resistance to change. While significant inroads have been made at the course and discipline level, more work needs to be done. The use of the UNPRME as a guide for delivering sustainability-focused management education has provided an opportunity to structure the change process and to provide support through partnerships.  相似文献   
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The purpose of this systematic review was to synthesise and evaluate the literature on the effects of classroom-based dynamic seating interventions on academic-related outcomes, among school-aged children and adolescents. A secondary aim was to examine the effects of interventions on students’ sedentary behaviour and physical activity levels. In September 2017, four electronic databases (PsycINFO, PubMed, SPORTDiscus, Web of Science) were searched and a total of 5138 titles and abstracts were reviewed. Studies that examined associations between a classroom-based dynamic seating intervention and at least one academic-related outcome in school-aged children or adolescents were included. A best-evidence synthesis and narrative approach was implemented to synthesise the evidence. Thirteen studies published between 2003 and 2017 were identified that met the inclusion criteria for the review. There is some evidence that classroom-based dynamic seating interventions could have positive effects on the in-seat behaviour, academic engagement and attention of school aged-children and adolescents, predominantly those with attention difficulties. It is currently unclear whether dynamic seating has positive effects on students’ on-task behaviour, disruptive behaviour, memory, concentration or academic achievement. No intervention was found to have a detrimental effect on academic-related outcomes. The findings come from low-quality to moderate-quality studies (M?=?60.62%; SD?=?10.44). Classroom-based dynamic seating could be a simple, effective health strategy to reduce students’ static sitting time without compromising student learning and academic performance. The current interventions need to be replicated with larger, adequately-powered RCT designs, valid and reliable outcome measures, and assessment of intervention fidelity.

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Nine faculty from 4 counselor education departments were interviewed regarding the evaluation criteria and decision‐making processes used during selection interviews. Ten characteristics were identified that participants looked for during interviews. There was a high degree of agreement among participants on the evaluation criteria they used. However, the same homogeneity was not found in decision‐making processes. Counselor educators seemed to use selection interviews to screen out applicants who were unsuitable rather than to select the most qualified applicants. Evaluation criteria and procedures should be refined to increase the benefits of using interviews in the selection process.  相似文献   
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The mathematics education community, in its call for reform, underscores the importance of mathematics instruction emphasizing the use of multiple representations in the presentation of concepts. The focus of the study was how teacher beliefs affect their ability to implement a multiple representations curriculum. The novice instructor's attitude remained neutral while the experienced instructor's attitude remained somewhat positive. Of the eight students in the study, six of them showed a more positive attitude towards technology use and multiple representations by the end of the semester. The students increased their calculator use in both classes, particularly in the experienced teacher's class. Implications for further research were that teacher training is essential if reform curricula are to be properly implemented.  相似文献   
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