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Linda Desbois Adrien Padirac Shohei Kaneda Anthony J. Genot Yannick Rondelez Didier Hober Dominique Collard Teruo Fujii 《Biomicrofluidics》2012,6(4)
Water-in-oil microdroplets offer microreactors for compartmentalized biochemical reactions with high throughput. Recently, the combination with a sol-gel switch ability, using agarose-in-oil microdroplets, has increased the range of possible applications, allowing for example the capture of amplicons in the gel phase for the preservation of monoclonality during a PCR reaction. Here, we report a new method for generating such agarose-in-oil microdroplets on a microfluidic device, with minimized inlet dead volume, on-chip cooling, and in situ monitoring of biochemical reactions within the gelified microbeads. We used a flow-focusing microchannel network and successfully generated agarose microdroplets at room temperature using the “push-pull” method. This method consists in pushing the oil continuous phase only, while suction is applied to the device outlet. The agarose phase present at the inlet is thus aspirated in the device, and segmented in microdroplets. The cooling system consists of two copper wires embedded in the microfluidic device. The transition from agarose microdroplets to microbeads provides additional stability and facilitated manipulation. We demonstrate the potential of this method by performing on-chip a temperature-triggered DNA isothermal amplification in agarose microbeads. Our device thus provides a new way to generate microbeads with high throughput and no dead volume for biochemical applications. 相似文献
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Optofluidic tweezer on a chip 总被引：1，自引：0，他引：1
A novel method to realize an optical tweezer involving optofluidic operation in a microchannel is proposed. To manipulate the optical tweezer, light from an optical fiber is passed through both PDMS (polydimethylsiloxane)-air surface lenses and an optofluidic region, which is located in a control channel. Two liquids with different refractive indices (RIs) are introduced into the control channel to form two different flow patterns (i.e., laminar and segmented flows), depending on the liquid compositions, the channel geometry, and the flow rates. By altering the shapes of the interface of the two liquids in the optofluidic region, we can continuously or intermittently control the optical paths of the light. To demonstrate the functionality of the proposed method, optical tweezer operations on a chip are performed. Changing the flow pattern of two liquids with different RIs in the optofluidic region results in successful trapping of a 25 μm diameter microsphere and its displacement by 15 μm. 相似文献
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Yeon Kyung Chee Kimberly R. Dash Shohei Noguchi Sue E. Levkoff 《Educational gerontology》2013,39(5):509-520
Despite the fact that Japan has developed many exemplary social policies for their expanding older population and programs to foster elderly workforce participation, shortage of personnel with a comprehensive understanding of gerontology has limited these efforts. In response to the need for greater gerontological education among the Japanese who will work in the allied health professions, we developed an intensive training program on “Successful and Productive Aging,” with two associate degree colleges in Japan. Emphasizing lessons learned from the United States experience, the three‐week program covered issues in health promotion/ disease prevention, health maintenance/independence, mechanisms of formal/ informal caregiving, and preparations for retirement. Instructional methods included didactic presentations by a multidisciplinary faculty, case‐based problem‐solving exercises, and field visits to various agencies and geriatric facilities in the greater Boston area. The educational program was field‐tested with 5 faculty from Japan who were asked to evaluate the program based on whether the learning objectives were met, perceptions of relevance of subject matters to students in Japan, and perceptions of teaching methods’ effectiveness. We present the results of this successful cross‐national collaboration, with emphasis on how information gathered is being integrated into teaching plans upon returning to Japan. 相似文献
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Satomi Izumi Taylor Cosby Steele Rogers Arleen Theresa Dodd Toshiko Kaneda Iku Nagasaki Yasuhiro Watanabe 《Journal of Early Childhood Teacher Education》2013,34(4):311-321
Abstract Early childhood teachers’ understandings of play were examined in a qualitative comparative study of 41 Japanese and 41 American participants. Qualitative analysis of the data yielded five themes: the power of living, the opportunity to learn and to develop, fun activities, child's work, and the opportunity to explore nature. Two themes emerged in regard to the meaning of play for adults: enjoyable and voluntary activities and child's play. Finally, we identified two themes regarding playfulness: state of heart and fun feelings. Teachers in both nations used rhetoric that is congruent with the current Zeitgeist of developmentally appropriate early education. One theme that differentiated the groups was the notion that play is children's work. While several Americans mentioned this slogan, this was not the case for the Japanese participants. In contrast, the theme of play as the power of living was more representative of Japanese teachers’ understandings of play, and this reflects the philosophy set forth by the Japanese Ministry of Education, Culture, Sports, Science, and Technology. 相似文献
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